ViewGene
Locus Name:
GeneID:
Location:

GeneID:810
TrivialName: PA14_39330
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_39330
PAO1 Ortholog Locus:PA1947
Sequence Length:1533
Protein Length:510
Start: 3502376
Stop: 3500844
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05335.1|AE004621_6 (AE004621) ribose transport protein RbsA [Pseudomonas aeruginosa PAO1]
Identities = 509/510 (99%), Positives = 510/510 (100%)
Sequence:
ATGTCTAGCGACGCATTGCCGCTGCTGAGCATCCGCGGGGTCGGCAAGAccTACGCGCAGCCGGTGCTGGCG
GAGATCGACCTGCAACTGTTCGGCGGCGAGGTGCTGGCCCTGACCGGCGAGAACGGCGCCGGCAAGAGCACC
CTGTCGAAGATCGTCGGCGGCCTGGAACGTCCCGGCGCCGGCAGCCTGGAGCTCCTCGGCCGGCCTTACGCC
CCCGCTAGCCGCCGCGAGGCCGAGGCGCTGGGCGTGCGCATGGTGATGCAGGAACTGAACCTGCTGCCGACC
CTGTCGGTGGCGGAGAACCTGTTCCTCCACGACCTGCCCCGGCGCGCCGGCTGGATCGACCGTCGGCGCCTG
CGCGCGGCGGCCCGCGAGGCGATGGCCCAGGTCGGCCTGGAGGCGATCGATCCCGACACGCTGGTCGGCGAC
CTCGGTATCGGCCACCAGCAGATGGTCGAGATCGCCCGCAACCTGATCGGCGACTGCCGCTTGCTGATCCTC
GACGAACCCACGGCGATGCTTACCGCGCGCGAAGTGGACATGCTCTTCGAGCAGGTCGAGCGGCTGCGCGAA
CGCGGCGTGGCGATCGTCTACATCTCCCATCGACTGGAGGAACTGGCGCGGATTTCGCAGCGCATCGCGGTG
TTGCGCGATGGCCGCCTGGTCTGCGTCGAGCCGATCGAGCGCTACGACGCCGACCAACTGGTGACCCTGATG
GTCGGCCGCGAGCTGGGCGAGCGCTTCGACCTGGGCCCACGGCAGACCGGTGCGCCGCTGCTGCGGGTGGAG
CGGTTGAGCCGGCGCGGCAAGGTGCATGAGGTGTCGTTCGAGGTGCGCGCGGGGGAGATCTTCGGCATTTCC
GGACTGATCGGCTCCGGGCGCACCGAATTGTTGCGCCTGATCTACGGCGCCGACCGCGCCGACGGTGGGCAG
GTCCTGCTCGGCGATCCGCCGCAGCGCCTGAGCCTGCGTTCGCCGGCCGACTCGGTGCGCCAGGGGGTGGCG
CTGATCACCGAGGACCGCAAGGGCGAGGGCCTGCTGCTGGACCAGTCGATCAGCGCCAACCTGGCCCTGGGC
AACCTGCCGGCGCTGGCGCGCCACGGGGTGATCGACCGGCGCCGCGAGGAAGCCCTGGCGCGGCGCCAGGTG
GAGGCGCTGCGGGTGCGCTGCGCGGACACCGCGCAGGCGGTGGGCGAACTGTCCGGCGGCAACCAGCAGAAG
GTGGTGATCGGCCGCTGGCTGGAACGCGACTGCCAGGTGCTGCTGTTCGACGAGCCGACCCGGGGCATCGAC
GTCGGCGCCAAGTTCGACATCTATGCCTTGCTCGCCGAACTGACCCGCCGGGGCAAGGCGCTGGTGGTGGTG
TCCAGCGACCTGCGCGAGCTGATGCTGATCTGCGATCGCATCGGCGTGCTCTCGGCGGGACGTATGGTGGAC
ACCTTCGAGCGCGATGCCTGGACCCAGGACGCGCTGCTGGCGGCGGCCTTCGCCGGTTACAAGAAACGCGAC
GCGCTGCTGGCGACGTCGTGA
Translation:
MSSDALPLLSIRGVGKTYAQPVLAEIDLQLFGGEVLALTGENGAGKSTLSKIVGGLERPGAGSLELLGRPYA
PASRREAEALGVRMVMQELNLLPTLSVAENLFLHDLPRRAGWIDRRRLRAAAREAMAQVGLEAIDPDTLVGD
LGIGHQQMVEIARNLIGDCRLLILDEPTAMLTAREVDMLFEQVERLRERGVAIVYISHRLEELARISQRIAV
LRDGRLVCVEPIERYDADQLVTLMVGRELGERFDLGPRQTGAPLLRVERLSRRGKVHEVSFEVRAGEIFGIS
GLIGSGRTELLRLIYGADRADGGQVLLGDPPQRLSLRSPADSVRQGVALITEDRKGEGLLLDQSISANLALG
NLPALARHGVIDRRREEALARRQVEALRVRCADTAQAVGELSGGNQQKVVIGRWLERDCQVLLFDEPTRGID
VGAKFDIYALLAELTRRGKALVVVSSDLRELMLICDRIGVLSAGRMVDTFERDAWTQDALLAAAFAGYKKRD
ALLATS*
AnnotationID:819GeneID:810
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 16:04:23
Gene Name:rbsA
Confidence Code:2
GeneProduct:ribose ABC transporter, ATP-binding protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1129,COG3845
Secondary Functional Category(ies):carbohydrate transport and metabolism
EC Number:3.6.3.17
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55879.1| (AE016864) ribose ABC transporter, ATP-binding protein [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 517

 Score =  747 bits (1928), Expect = 0.0
 Identities = 376/500 (75%), Positives = 437/500 (87%)

>gb|AAN68067.1|AE016439_2 (AE016783) ribose ABC transporter, ATP-binding protein [Pseudomonas
           putida KT2440]
          Length = 524

 Score =  738 bits (1906), Expect = 0.0
 Identities = 380/500 (76%), Positives = 426/500 (85%)

>emb|CAD74539.1| (BX294143) ribose transport ATP-binding protein RbsA [Pirellula
           sp.]
          Length = 522

 Score =  411 bits (1057), Expect = e-114
 Identities = 237/506 (46%), Positives = 316/506 (62%), Gaps = 16/506 (3%)








 
Structural Features:
COG1129, MglA, ABC-type sugar transport system, ATPase component 
[Carbohydrate transport and metabolism]
CD-Length = 500 residues, 100.0% aligned
Score =  566 bits (1461), Expect = 2e-162
Identities = 259/503 (51%), Positives = 348/503 (69%), Gaps = 7/503 (1%)

COG3845, COG3845, ABC-type uncharacterized transport systems, ATPase components 
CD-Length = 501 residues,  98.4% aligned
Score =  405 bits (1043), Expect = 5e-114
Identities = 212/495 (42%), Positives = 296/495 (59%), Gaps = 11/495 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1623




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18765




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32772




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:810_cdd.html


    		




 
URL:

















    ReferenceID:34351




  
Author/Investigator(s):
        Bell AW, Buckel SD, Groarke JM, Hope JN, Kingsley DH, Hermodson MA.





  
Title:
        The nucleotide sequences of the rbsD, rbsA, and rbsC genes of Escherichia coli K12.




  
PubMed:
        3011793


MedLine:
        




    
Source:
J Biol Chem. 1986 Jun 15;261(17):7652-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3011793

















    ReferenceID:34352




  
Author/Investigator(s):
        Iida A, Harayama S, Iino T, Hazelbauer GL.





  
Title:
        Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12.




  
PubMed:
        6327617


MedLine:
        




    
Source:
J Bacteriol. 1984 May;158(2):674-82.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6327617

















    ReferenceID:34353




  
Author/Investigator(s):
        Iida A, Harayama S, Iino T, Hazelbauer GL.





  
Title:
        Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12.




  
PubMed:
        6327617


MedLine:
        




    
Source:
J Bacteriol. 1984 May;158(2):674-82.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6327617

















    ReferenceID:34354




  
Author/Investigator(s):
        Barroga CF, Zhang H, Wajih N, Bouyer JH, Hermodson MA.





  
Title:
        The proteins encoded by the rbs operon of Escherichia coli: I. Overproduction, purification, characterization, and functional analysis of RbsA.




  
PubMed:
        8762140


MedLine:
        




    
Source:
Protein Sci. 1996 Jun;5(6):1093-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8762140









           
Homologs By Global Alignment 

           

       



     
Gene ID:810
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1453
                           PA1947_tr
                           translation of PA1947
                           510
                           99.80
                           99.99
                           0
                           2527.0
     

     
     
53039
                           gb|AAG05335.1|AE004621_6
                           (AE004621) ribose transport protein RbsA [Pseudomonas aeruginosa PAO1]
                           510
                           99.80
                           99.99
                           0
                           2527.0
     

     
     
53040
                           gb|AAO55879.1|
                           (AE016864) ribose ABC transporter, ATP-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           517
                           73.30
                           85.49
                           7
                           1928.0
     

     
     
53041
                           gb|AAN68067.1|AE016439_2
                           (AE016783) ribose ABC transporter, ATP-binding protein [Pseudomonas putida KT2440]
                           524
                           72.90
                           82.06
                           14
                           1902.0
     

     
     
53042
                           emb|CAD14718.1|
                           (AL646062) PROBABLE ATP-BINDING ABC TRANSPORTER PROTEIN [Ralstonia solanacearum]
                           526
                           59.69
                           72.05
                           19
                           1509.0
     

     






      

          
GC ORFID: 43812How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1947Glimmer Score: 

          
Start: 1451535Stop: 1453067

          
Length: 1533


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1947 translation of PA1947Homolog Bit Score 978.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene101 (ORF: BLASTX 319.0), t_PA0136 (ORF: BLASTX 276.0), psyr_15may02_Scaffold2_revised_gene3857 (ORF: BLASTX 228.0), t_PA1947 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTAGCGACGCATTGCCGCTGCTGAGCATCCGCGGGGTCGGCAAGAccTACGCGCAGCCGGTGCTGGCG 
GAGATCGACCTGCAACTGTTCGGCGGCGAGGTGCTGGCCCTGACCGGCGAGAACGGCGCCGGCAAGAGCACC 
CTGTCGAAGATCGTCGGCGGCCTGGAACGTCCCGGCGCCGGCAGCCTGGAGCTCCTCGGCCGGCCTTACGCC 
CCCGCTAGCCGCCGCGAGGCCGAGGCGCTGGGCGTGCGCATGGTGATGCAGGAACTGAACCTGCTGCCGACC 
CTGTCGGTGGCGGAGAACCTGTTCCTCCACGACCTGCCCCGGCGCGCCGGCTGGATCGACCGTCGGCGCCTG 
CGCGCGGCGGCCCGCGAGGCGATGGCCCAGGTCGGCCTGGAGGCGATCGATCCCGACACGCTGGTCGGCGAC 
CTCGGTATCGGCCACCAGCAGATGGTCGAGATCGCCCGCAACCTGATCGGCGACTGCCGCTTGCTGATCCTC 
GACGAACCCACGGCGATGCTTACCGCGCGCGAAGTGGACATGCTCTTCGAGCAGGTCGAGCGGCTGCGCGAA 
CGCGGCGTGGCGATCGTCTACATCTCCCATCGACTGGAGGAACTGGCGCGGATTTCGCAGCGCATCGCGGTG 
TTGCGCGATGGCCGCCTGGTCTGCGTCGAGCCGATCGAGCGCTACGACGCCGACCAACTGGTGACCCTGATG 
GTCGGCCGCGAGCTGGGCGAGCGCTTCGACCTGGGCCCACGGCAGACCGGTGCGCCGCTGCTGCGGGTGGAG 
CGGTTGAGCCGGCGCGGCAAGGTGCATGAGGTGTCGTTCGAGGTGCGCGCGGGGGAGATCTTCGGCATTTCC 
GGACTGATCGGCTCCGGGCGCACCGAATTGTTGCGCCTGATCTACGGCGCCGACCGCGCCGACGGTGGGCAG 
GTCCTGCTCGGCGATCCGCCGCAGCGCCTGAGCCTGCGTTCGCCGGCCGACTCGGTGCGCCAGGGGGTGGCG 
CTGATCACCGAGGACCGCAAGGGCGAGGGCCTGCTGCTGGACCAGTCGATCAGCGCCAACCTGGCCCTGGGC 
AACCTGCCGGCGCTGGCGCGCCACGGGGTGATCGACCGGCGCCGCGAGGAAGCCCTGGCGCGGCGCCAGGTG 
GAGGCGCTGCGGGTGCGCTGCGCGGACACCGCGCAGGCGGTGGGCGAACTGTCCGGCGGCAACCAGCAGAAG 
GTGGTGATCGGCCGCTGGCTGGAACGCGACTGCCAGGTGCTGCTGTTCGACGAGCCGACCCGGGGCATCGAC 
GTCGGCGCCAAGTTCGACATCTATGCCTTGCTCGCCGAACTGACCCGCCGGGGCAAGGCGCTGGTGGTGGTG 
TCCAGCGACCTGCGCGAGCTGATGCTGATCTGCGATCGCATCGGCGTGCTCTCGGCGGGACGTATGGTGGAC 
ACCTTCGAGCGCGATGCCTGGACCCAGGACGCGCTGCTGGCGGCGGCCTTCGCCGGTTACAAGAAACGCGAC 
GCGCTGCTGGCGACGTCGTGA