ViewGene
Locus Name:
GeneID:
Location:

GeneID:805
TrivialName: PA14_67320
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67320
PAO1 Ortholog Locus:PA5098
Sequence Length:1530
Protein Length:509
Start: 6013685
Stop: 6012156
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08483.1|AE004922_8 (AE004922) histidine ammonia-lyase [Pseudomonas aeruginosa PAO1]
Identities = 508/509 (99%), Positives = 509/509 (100%)
Sequence:
ATGAGCCTGCATCTGAAACCCGGCCAGCTGACCCTGGCCGACCTGCGCCAAGCCTACCTGGCCCCCGTGCGC
CTGAGCCTGGATCCCAGCGCCGACGCGCCGGTCGCCGCCAGCGTCGCCTGTGTCGAGAACATCATCGCCGAG
GGCCGCACCGCCTACGGCATCAATACCGGTTTCGGCCTGCTGGCCTCGACGCGGATCTCCCCGGCCGACCTG
GAGAAGCTCCAGCGCTCCATCGTCCTGTCCCATGCCGCCGGCGTCGGCGAGGCGCTGGACGACGCCATGGTG
CGGCTGGTCATGCTGCTCAAGGTGAACAGCCTGGCTCGCGGGTTCTCCGGCATCCGCCGCAAGGTGATCGAC
GCGCTGATCGCGCTGATCAACGCCGAGGTCTATCCGCACATCCCGCTGAAAGGCTCGGTGGGCGCCTCCGGC
GACCTGGCGCCGCTGGCGCACATGTCGCTGGTGCTGATCGGCGAAAGCCGGGCGCGCCATCGCGGTGAGTGG
CTGCCGGCCGCCGAGGCGCTGGCGGTGGCCGGGCTGGAGCCGCTGACCCTGGCGGCGAAGGAAGGCCTGGCC
CTGCTCAATGGTACCCAGGTGTCCACCGCCTACGCGCTGCGCGGGTTGTTCGAGGCCGAGGACCTGTTCGCC
GCCGCCACCGTCTGCGGCGGGCTCAGCGTCGAGGCCATGCTCGGTTCGCGGGCGCCGTTCGATGCGCGCATC
CACGCCGCGCGCGGCCAGCGTGGGCAGATCGACGTGGCCGCGGCCTATCGCGACCTGCTCACCGCCAGCAGC
GAGGTGGCGCGTTCCCATGAGAAGTGCGACAAGGTCCAGGACCCCTATTCGCTGCGTTGCCAGCCGCAGGTG
ATGGGCGCCTGCCTGACCCAGATGCGCCAGGCCGCCGAGGTGCTGGAGATCGAAGCCAACGCGGTGTCCGAC
AACCCGCTGGTATTCGCCGCCGAGGGCGACGTGATCTCCGGCGGCAACTTCCACGCCGAACCGGTGGCGATG
GCCGCCGACAACCTGGCCCTGGCGTTGGCCGAGATCGGTTCGCTGTCGGAACGGCGCATCTCGCTGATGATG
GACATGCACATGTCGCAGTTGCCGCCGTTCCTGGTGGCCAACGGCGGGGTCAACTCCGGCTTCATGATCGCC
CAGGTCACCGCCGCCGCGCTGGCCAGCGACAACAAGGCCCTGGCCCATCCGGCCAGCGTCGACAGCCTGCCG
ACCTCGGCCAACCAGGAAGACCACGTGTCGATGGCGCCGAACGCCGGCAAACGGCTCTGGGCGATGGCCGAG
AACGTCCGCGGGATTCTCGCCGTGGAATGGCTGGGCGCCTGCCAGGGCCTGGACTTCCGTGAGGGCCTGAAG
AGTTCGCCGAAGCTGGAGCAGGCTCGCCGCCTGCTGCGCGACAAGGTGCCGTACTACCAGGAGGATCGCTTC
TTCGCCCCTGACATCGAGGCCGCCAGCCAGTTGCTCGCCAGCGGTTGCCTGAACGCGCTGCTGCCGGCCCGG
CTGTTGCCGAGCCTTTGA
Translation:
MSLHLKPGQLTLADLRQAYLAPVRLSLDPSADAPVAASVACVENIIAEGRTAYGINTGFGLLASTRISPADL
EKLQRSIVLSHAAGVGEALDDAMVRLVMLLKVNSLARGFSGIRRKVIDALIALINAEVYPHIPLKGSVGASG
DLAPLAHMSLVLIGESRARHRGEWLPAAEALAVAGLEPLTLAAKEGLALLNGTQVSTAYALRGLFEAEDLFA
AATVCGGLSVEAMLGSRAPFDARIHAARGQRGQIDVAAAYRDLLTASSEVARSHEKCDKVQDPYSLRCQPQV
MGACLTQMRQAAEVLEIEANAVSDNPLVFAAEGDVISGGNFHAEPVAMAADNLALALAEIGSLSERRISLMM
DMHMSQLPPFLVANGGVNSGFMIAQVTAAALASDNKALAHPASVDSLPTSANQEDHVSMAPNAGKRLWAMAE
NVRGILAVEWLGACQGLDFREGLKSSPKLEQARRLLRDKVPYYQEDRFFAPDIEAASQLLASGCLNALLPAR
LLPSL*
AnnotationID:814GeneID:805
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 17:05:16
Gene Name:hutH
Confidence Code:2
GeneProduct:histidine ammonia-lyase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd01441,pfam00221,cd00332,cd01442
Secondary Functional Category(ies):
EC Number:4.3.1.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58701.1| (AE016875) histidine ammonia-lyase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 515

 Score =  826 bits (2133), Expect = 0.0
 Identities = 424/508 (83%), Positives = 460/508 (90%)

>emb|CAD19072.1| (AJ421809) putative histidine ammonia lyase [Pseudomonas
           fluorescens]
          Length = 514

 Score =  824 bits (2129), Expect = 0.0
 Identities = 423/508 (83%), Positives = 458/508 (90%)

>gb|AAM87581.1|AE014005_3 (AE014005) putative histidine ammonia-lyase [Yersinia pestis KIM]
          Length = 510

 Score =  772 bits (1994), Expect = 0.0
 Identities = 395/508 (77%), Positives = 443/508 (87%)






 
Structural Features:
cd01441, HAL, Histidine ammonia-lyase (HAL) catalyzes the first step in the 
degradation of histidine to glutamate.
CD-Length = 486 residues, 100.0% aligned
Score =  666 bits (1721), Expect = 0.0

pfam00221, PAL, Phenylalanine and histidine ammonia-lyase.
CD-Length = 487 residues,  99.6% aligned
Score =  553 bits (1425), Expect = 3e-158
Identities = 238/485 (49%), Positives = 312/485 (64%), Gaps = 11/485 (2%)

cd00332, PAL-HAL, Histidine ammonia-lyase (HAL) and phenylalanine 
ammonia-lyase (PAL). 
CD-Length = 508 residues, 100.0% aligned
Score =  524 bits (1350), Expect = 1e-149

cd01442, PAL, Phenylalanine ammonia-lyase (PAL)
CD-Length = 510 residues,  99.4% aligned
Score =  328 bits (843), Expect = 7e-91






   
Genomic Context:






   
Comment:


















    ReferenceID:1613




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18755




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34970




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:805_cdd.html


    		




 
URL:

















    ReferenceID:35813




  
Author/Investigator(s):
        Consevage MW, Phillips AT.





  
Title:
        Sequence analysis of the hutH gene encoding histidine ammonia-lyase in Pseudomonas putida.




  
PubMed:
        2332400


MedLine:
        




    
Source:
J Bacteriol. 1990 May;172(5):2224-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2332400

















    ReferenceID:35814




  
Author/Investigator(s):
        Wu PC, Kroening TA, White PJ, Kendrick KE.





  
Title:
        Purification of histidase from Streptomyces griseus and nucleotide sequence of the hutH structural gene.




  
PubMed:
        1537807


MedLine:
        




    
Source:
J Bacteriol. 1992 Mar;174(5):1647-55.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1537807









           
Homologs By Global Alignment 

           

       



     
Gene ID:805
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1436
                           PA5098_tr
                           translation of PA5098
                           509
                           99.80
                           99.99
                           0
                           2532.0
     

     
     
52693
                           gb|AAG08483.1|AE004922_8
                           (AE004922) histidine ammonia-lyase [Pseudomonas aeruginosa PAO1]
                           509
                           99.80
                           99.99
                           0
                           2532.0
     

     
     
52694
                           gb|AAO58701.1|
                           (AE016875) histidine ammonia-lyase [Pseudomonas syringae pv. tomato str. DC3000]
                           515
                           82.33
                           89.32
                           6
                           2132.0
     

     
     
52695
                           emb|CAD19072.1|
                           (AJ421809) putative histidine ammonia lyase [Pseudomonas fluorescens]
                           514
                           82.29
                           89.10
                           5
                           2128.0
     

     
     
52696
                           gb|AAN70597.1|AE016702_6
                           (AE016793) histidine ammonia-lyase [Pseudomonas putida KT2440]
                           510
                           81.56
                           88.62
                           1
                           2104.0
     

     






      

          
GC ORFID: 43807How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5098Glimmer Score: 

          
Start: 5277370Stop: 5275841

          
Length: 1530


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5098 translation of PA5098Homolog Bit Score 979.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6903 (ORF: BLASTX 826.0), t_PA5093 (ORF: BLASTX 327.0), psyr_15may02_Scaffold6_revised_gene6902 (ORF: BLASTX 322.0), t_PA5098 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCTGCATCTGAAACCCGGCCAGCTGACCCTGGCCGACCTGCGCCAAGCCTACCTGGCCCCCGTGCGC 
CTGAGCCTGGATCCCAGCGCCGACGCGCCGGTCGCCGCCAGCGTCGCCTGTGTCGAGAACATCATCGCCGAG 
GGCCGCACCGCCTACGGCATCAATACCGGTTTCGGCCTGCTGGCCTCGACGCGGATCTCCCCGGCCGACCTG 
GAGAAGCTCCAGCGCTCCATCGTCCTGTCCCATGCCGCCGGCGTCGGCGAGGCGCTGGACGACGCCATGGTG 
CGGCTGGTCATGCTGCTCAAGGTGAACAGCCTGGCTCGCGGGTTCTCCGGCATCCGCCGCAAGGTGATCGAC 
GCGCTGATCGCGCTGATCAACGCCGAGGTCTATCCGCACATCCCGCTGAAAGGCTCGGTGGGCGCCTCCGGC 
GACCTGGCGCCGCTGGCGCACATGTCGCTGGTGCTGATCGGCGAAAGCCGGGCGCGCCATCGCGGTGAGTGG 
CTGCCGGCCGCCGAGGCGCTGGCGGTGGCCGGGCTGGAGCCGCTGACCCTGGCGGCGAAGGAAGGCCTGGCC 
CTGCTCAATGGTACCCAGGTGTCCACCGCCTACGCGCTGCGCGGGTTGTTCGAGGCCGAGGACCTGTTCGCC 
GCCGCCACCGTCTGCGGCGGGCTCAGCGTCGAGGCCATGCTCGGTTCGCGGGCGCCGTTCGATGCGCGCATC 
CACGCCGCGCGCGGCCAGCGTGGGCAGATCGACGTGGCCGCGGCCTATCGCGACCTGCTCACCGCCAGCAGC 
GAGGTGGCGCGTTCCCATGAGAAGTGCGACAAGGTCCAGGACCCCTATTCGCTGCGTTGCCAGCCGCAGGTG 
ATGGGCGCCTGCCTGACCCAGATGCGCCAGGCCGCCGAGGTGCTGGAGATCGAAGCCAACGCGGTGTCCGAC 
AACCCGCTGGTATTCGCCGCCGAGGGCGACGTGATCTCCGGCGGCAACTTCCACGCCGAACCGGTGGCGATG 
GCCGCCGACAACCTGGCCCTGGCGTTGGCCGAGATCGGTTCGCTGTCGGAACGGCGCATCTCGCTGATGATG 
GACATGCACATGTCGCAGTTGCCGCCGTTCCTGGTGGCCAACGGCGGGGTCAACTCCGGCTTCATGATCGCC 
CAGGTCACCGCCGCCGCGCTGGCCAGCGACAACAAGGCCCTGGCCCATCCGGCCAGCGTCGACAGCCTGCCG 
ACCTCGGCCAACCAGGAAGACCACGTGTCGATGGCGCCGAACGCCGGCAAACGGCTCTGGGCGATGGCCGAG 
AACGTCCGCGGGATTCTCGCCGTGGAATGGCTGGGCGCCTGCCAGGGCCTGGACTTCCGTGAGGGCCTGAAG 
AGTTCGCCGAAGCTGGAGCAGGCTCGCCGCCTGCTGCGCGACAAGGTGCCGTACTACCAGGAGGATCGCTTC 
TTCGCCCCTGACATCGAGGCCGCCAGCCAGTTGCTCGCCAGCGGTTGCCTGAACGCGCTGCTGCCGGCCCGG 
CTGTTGCCGAGCCTTTGA