ViewGene
Locus Name:
GeneID:
Location:

GeneID:804
TrivialName: PA14_34360
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_34360
PAO1 Ortholog Locus:PA2342
Sequence Length:1476
Protein Length:491
Start: 3058156
Stop: 3056681
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05730.1|AE004660_6 (AE004660) mannitol dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 486/491 (98%), Positives = 486/491 (98%)
Sequence:
ATGAAACTCAACCGGCAGCACCTCCCCCTGCTGGCGACCGCTGTCGCCCGCCCGTCCTACGACCCCGCGCAA
CTGCGCCAGGGCATCGTCCACATCGGCGTCGGCGGCTTCCACCGCGCGCACCAGGCGGCCTACACCGACGCC
CTGATGAACCGCGGCGAAGCCCTCGACTGGGCGATCTGCGGCGCCGGCCTGCGCAGCGACGACCGCGCCATG
CGCGACGCCCTGGCCGCCCAGGACTACCTCTACACCCTCTACGAGCTGGGCGACCAACCGGACACCGAGGTT
CGCGTGATCGGCGCCATCAGCGGCATGCTGCTCGCCGAGGACGGCGCCGAGGCGCTGCTGGAAAAGCTTGCC
GAGCCGGCCATCCGCATCGTCTCGCTGACCATCACCGAAGGCGGCTACTGCATCGACGATGGCAGCGGCGAG
TTCCTCGCCGAGTTGCCGCTGGTGCGCCACGACCTGGCCAACCCGCGGACCCCGCGCGGGGTCTTCGGCTTT
CTCTGCGAGGCTCTGCGGCGGCGCCGCGACACAGGCGTGCCGGCGTTCACCGTGATGTCCTGCGACAACCTC
CCGCACAACGGCGAGGTGGCGCGCAAGGCGCTGCTGGCCTTCGCCGAACGGCTCGACCCGGGGCTGGCGCGC
TGGATCGCGACGCACGTCAGCTTTCCCAACGCCATGGTCGACCGCATCACCCCGATGACCAGCCCCGCCCAC
CGCCGCCAACTGGCCCAACGGCACGGCGTGGAAGACGCCTGGCCGGTGGTCTGCGAACCCTTCGTGCAATGG
GTGCTGGAAGATCATTTCAGCGCTGGGCGCCCGGCCTGGGAAAAGGTCGGCGTGCAGTTCACCGACGACGTC
ACGCCCTACGAGGAGATGAAGATCGGCCTGCTCAACGGCAGCCACCTGGCGCTCACCTACCTGGGCTTCCTG
CGCGGCTACCGCTTCGTCCACGAGACCCTCGGCGACCCGCTGCTGCGCCGCTACGTGCGGACCTTCATGGAC
CGCGACGTGGCCCCACTGCTGGCGCCGGTACCGGGGATCGACCTGGAGCGCTACAAGGACAGCCTGGTCGAG
CGCTTCGCCAACCGCGCCATCGCCGACCAACTGGAACGGGTCTGCTCGGACGGCTCGTCGAAGTTTCCCAAG
TTCATCGTCCCCACCGCCAATCGGCTGATCGCCGCCGGCCGCCCACTGGAACGGGTGGCCCTGGTGGTGGCG
GCCTGGGCGCTGTACCTGGGCGGGGTCGACGAGCATGGCGAGCACTACCCGATTCCCGATCCGCGCGCCGCC
GAATGCCAGGCGCTGGTTGCCGAACGCCACGGCCTGGGCCGGCGCCTGCTCGGCGTGGAGACCGTGTTCGGC
CCGGCGATCCCGGCCTCGGCGGCCTTCGTCGAGGCCTTCGAGCGCCTCTACGACAGCCTGCGCCGACACGGC
GTCAGCGAAACCCTGCGCCAGGTACTCGGCGAATGA
Translation:
MKLNRQHLPLLATAVARPSYDPAQLRQGIVHIGVGGFHRAHQAAYTDALMNRGEALDWAICGAGLRSDDRAM
RDALAAQDYLYTLYELGDQPDTEVRVIGAISGMLLAEDGAEALLEKLAEPAIRIVSLTITEGGYCIDDGSGE
FLAELPLVRHDLANPRTPRGVFGFLCEALRRRRDTGVPAFTVMSCDNLPHNGEVARKALLAFAERLDPGLAR
WIATHVSFPNAMVDRITPMTSPAHRRQLAQRHGVEDAWPVVCEPFVQWVLEDHFSAGRPAWEKVGVQFTDDV
TPYEEMKIGLLNGSHLALTYLGFLRGYRFVHETLGDPLLRRYVRTFMDRDVAPLLAPVPGIDLERYKDSLVE
RFANRAIADQLERVCSDGSSKFPKFIVPTANRLIAAGRPLERVALVVAAWALYLGGVDEHGEHYPIPDPRAA
ECQALVAERHGLGRRLLGVETVFGPAIPASAAFVEAFERLYDSLRRHGVSETLRQVLGE*
AnnotationID:813GeneID:804
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 07:07:38
Gene Name:mtlD
Confidence Code:2
GeneProduct:mannitol dehydrogenase
Cell Localization:()
Synonyms:uxuB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:D-mannonate oxidoreductase
Functional Category Confidence Code:5
COGs:COG0246,pfam01232
Secondary Functional Category(ies):
EC Number:1.1.1.17
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC04472.1| (AF007800) mannitol dehydrogenase [Pseudomonas fluorescens]
          Length = 493

 Score =  714 bits (1844), Expect = 0.0
 Identities = 355/489 (72%), Positives = 396/489 (80%)

>gb|AAO56205.1| (AE016865) D-mannonate oxidoreductase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 493

 Score =  690 bits (1781), Expect = 0.0
 Identities = 342/489 (69%), Positives = 387/489 (79%)

>dbj|BAA02591.1| (D13329) Mannonate oxidoreductase [Escherichia coli]
          Length = 486

 Score =  379 bits (972), Expect = e-104
 Identities = 204/473 (43%), Positives = 278/473 (58%), Gaps = 6/473 (1%)






 
Structural Features:
COG0246, MtlD, Mannitol-1-phosphate/altronate dehydrogenases 
[Carbohydrate transport and metabolism]
CD-Length = 473 residues, 100.0% aligned
Score =  536 bits (1382), Expect = 2e-153
Identities = 234/480 (48%), Positives = 299/480 (62%), Gaps = 10/480 (2%)

pfam01232, Mannitol_dh, Mannitol dehydrogenase.
CD-Length = 384 residues,  99.7% aligned
Score =  440 bits (1133), Expect = 2e-124
Identities = 187/418 (44%), Positives = 241/418 (57%), Gaps = 36/418 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1611




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18753




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32456




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:804_cdd.html


    		




 
URL:

















    ReferenceID:34471




  
Author/Investigator(s):
        Davis T, Yamada M, Elgort M, Saier MH Jr.





  
Title:
        Nucleotide sequence of the mannitol (mtl) operon in Escherichia coli.




  
PubMed:
        3135464


MedLine:
        




    
Source:
Mol Microbiol. 1988 May;2(3):405-12.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3135464

















    ReferenceID:34472




  
Author/Investigator(s):
        Jiang W, Wu LF, Tomich J, Saier MH Jr, Niehaus WG.





  
Title:
        Corrected sequence of the mannitol (mtl) operon in Escherichia coli.




  
PubMed:
        1964486


MedLine:
        




    
Source:
Mol Microbiol. 1990 Nov;4(11):2003-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1964486

















    ReferenceID:34473




  
Author/Investigator(s):
        Fischer R, von Strandmann RP, Hengstenberg W.





  
Title:
        Mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system of Enterococcus faecalis: molecular cloning and nucleotide sequences of the enzyme IIIMtl gene and the mannitol-1-phosphate dehydrogenase gene, expression in Escherichia coli, and c




  
PubMed:
        1904856


MedLine:
        




    
Source:
J Bacteriol. 1991 Jun;173(12):3709-15.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1904856

















    ReferenceID:34474




  
Author/Investigator(s):
        Honeyman AL, Curtiss R 3rd.





  
Title:
        Isolation, characterization, and nucleotide sequence of the Streptococcus mutans mannitol-phosphate dehydrogenase gene and the mannitol-specific factor III gene of the phosphoenolpyruvate phosphotransferase system.




  
PubMed:
        1322373


MedLine:
        




    
Source:
Infect Immun. 1992 Aug;60(8):3369-75.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1322373

















    ReferenceID:34475




  
Author/Investigator(s):
        Henstra SA, Tolner B, ten Hoeve Duurkens RH, Konings WN, Robillard GT.





  
Title:
        Cloning, expression, and isolation of the mannitol transport protein from the thermophilic bacterium Bacillus stearothermophilus.




  
PubMed:
        8824601


MedLine:
        




    
Source:
J Bacteriol. 1996 Oct;178(19):5586-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8824601

















    ReferenceID:34476




  
Author/Investigator(s):
        Brunker P, Altenbuchner J, Kulbe KD, Mattes R.





  
Title:
        Cloning, nucleotide sequence and expression of a mannitol dehydrogenase gene from Pseudomonas fluorescens DSM 50106 in Escherichia coli.




  
PubMed:
        9116029


MedLine:
        




    
Source:
Biochim Biophys Acta. 1997 Mar 20;1351(1-2):157-67.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9116029

















    ReferenceID:34477




  
Author/Investigator(s):
        Otte S, Lengeler JW.





  
Title:
        The mtl genes and the mannitol-1-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026.




  
PubMed:
        11164312


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2001 Jan 15;194(2):221-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11164312









           
Homologs By Global Alignment 

           

       



     
Gene ID:804
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1435
                           PA2342_tr
                           translation of PA2342
                           491
                           98.98
                           98.98
                           0
                           2531.0
     

     
     
52635
                           gb|AAG05730.1|AE004660_6
                           (AE004660) mannitol dehydrogenase [Pseudomonas aeruginosa PAO1]
                           491
                           98.98
                           98.98
                           0
                           2531.0
     

     
     
52636
                           gb|AAC04472.1|
                           (AF007800) mannitol dehydrogenase [Pseudomonas fluorescens]
                           493
                           72.00
                           80.52
                           2
                           1843.0
     

     
     
52637
                           gb|AAO56205.1|
                           (AE016865) D-mannonate oxidoreductase [Pseudomonas syringae pv. tomato str. DC3000]
                           493
                           69.37
                           78.49
                           2
                           1781.0
     

     
     
52638
                           emb|CAD72610.1|
                           (BX294137) D-mannonate oxidoreductase [Pirellula sp.] [Pirellula sp. 1]
                           503
                           45.92
                           60.63
                           13
                           1134.0
     

     






      

          
GC ORFID: 43806How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2342Glimmer Score: 

          
Start: 1895760Stop: 1897235

          
Length: 1476


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2342 translation of PA2342Homolog Bit Score 979.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3069 (ORF: BLASTX 694.0), t_PA2342 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAACTCAACCGGCAGCACCTCCCCCTGCTGGCGACCGCTGTCGCCCGCCCGTCCTACGACCCCGCGCAA 
CTGCGCCAGGGCATCGTCCACATCGGCGTCGGCGGCTTCCACCGCGCGCACCAGGCGGCCTACACCGACGCC 
CTGATGAACCGCGGCGAAGCCCTCGACTGGGCGATCTGCGGCGCCGGCCTGCGCAGCGACGACCGCGCCATG 
CGCGACGCCCTGGCCGCCCAGGACTACCTCTACACCCTCTACGAGCTGGGCGACCAACCGGACACCGAGGTT 
CGCGTGATCGGCGCCATCAGCGGCATGCTGCTCGCCGAGGACGGCGCCGAGGCGCTGCTGGAAAAGCTTGCC 
GAGCCGGCCATCCGCATCGTCTCGCTGACCATCACCGAAGGCGGCTACTGCATCGACGATGGCAGCGGCGAG 
TTCCTCGCCGAGTTGCCGCTGGTGCGCCACGACCTGGCCAACCCGCGGACCCCGCGCGGGGTCTTCGGCTTT 
CTCTGCGAGGCTCTGCGGCGGCGCCGCGACACAGGCGTGCCGGCGTTCACCGTGATGTCCTGCGACAACCTC 
CCGCACAACGGCGAGGTGGCGCGCAAGGCGCTGCTGGCCTTCGCCGAACGGCTCGACCCGGGGCTGGCGCGC 
TGGATCGCGACGCACGTCAGCTTTCCCAACGCCATGGTCGACCGCATCACCCCGATGACCAGCCCCGCCCAC 
CGCCGCCAACTGGCCCAACGGCACGGCGTGGAAGACGCCTGGCCGGTGGTCTGCGAACCCTTCGTGCAATGG 
GTGCTGGAAGATCATTTCAGCGCTGGGCGCCCGGCCTGGGAAAAGGTCGGCGTGCAGTTCACCGACGACGTC 
ACGCCCTACGAGGAGATGAAGATCGGCCTGCTCAACGGCAGCCACCTGGCGCTCACCTACCTGGGCTTCCTG 
CGCGGCTACCGCTTCGTCCACGAGACCCTCGGCGACCCGCTGCTGCGCCGCTACGTGCGGACCTTCATGGAC 
CGCGACGTGGCCCCACTGCTGGCGCCGGTACCGGGGATCGACCTGGAGCGCTACAAGGACAGCCTGGTCGAG 
CGCTTCGCCAACCGCGCCATCGCCGACCAACTGGAACGGGTCTGCTCGGACGGCTCGTCGAAGTTTCCCAAG 
TTCATCGTCCCCACCGCCAATCGGCTGATCGCCGCCGGCCGCCCACTGGAACGGGTGGCCCTGGTGGTGGCG 
GCCTGGGCGCTGTACCTGGGCGGGGTCGACGAGCATGGCGAGCACTACCCGATTCCCGATCCGCGCGCCGCC 
GAATGCCAGGCGCTGGTTGCCGAACGCCACGGCCTGGGCCGGCGCCTGCTCGGCGTGGAGACCGTGTTCGGC 
CCGGCGATCCCGGCCTCGGCGGCCTTCGTCGAGGCCTTCGAGCGCCTCTACGACAGCCTGCGCCGACACGGC 
GTCAGCGAAACCCTGCGCCAGGTACTCGGCGAATGA