ViewGene
Locus Name:
GeneID:
Location:

GeneID:80
TrivialName: PA14_14440
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14440
PAO1 Ortholog Locus:PA3834
Sequence Length:2853
Protein Length:950
Start: 1234433
Stop: 1231581
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07221.1|AE004800_10 (AE004800) valyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 945/950 (99%), Positives = 947/950 (99%)
Sequence:
ATGGACAAGACTTACCAGCCGCACGCCATCGAGACTTCCTGGTACGAGACCTGGGAGTCCAACGACTACTTC
GCTCCGAGCGGCGAAGGCCAGCCCTACACCATCATGATCCCGCCGCCGAACGTGACCGGCAGCCTGCACATG
GGCCATGGCTTCAACAACGCGATAATGGACGCGCTGATCCGCTACCGCCGCATGCAGGGCCGCAACACCCTG
TGGCAGCCGGGCACCGATCACGCCGGCATCGCCACCCAGATGGTGGTGGAGCGCCAGCTCGGCGCCCAGGGC
GTGAGCCGCCACGACCTCGGCCGGGAGAAGTTCCTGGAGAAGGTCTGGGAATGGAAGGAACAGTCCGGCGGC
AACATCACCCGGCAGATTCGTCGCCTGGGCTCCTCGGTGGACTGGTCGCGCGAGCGCTTCACCATGGACGAC
GGCCTCTCCGAAGCGGTCAAGGAAGCCTTCGTGCGCCTGCATGAAGACGGCCTGATCTACCGCGGCAAACGC
CTGGTGAACTGGGACACCAAGCTGCACACCGCGATTTCCGACCTGGAAGTGGAAAACCACGACGAGAAGGGC
CACCTCTGGCACCTGCGCTACCCGCTGGTGAAAGGCGCGAAGACCAGCGAAGGCCTGGACTACCTGGTGGTC
GCCACTACCCGTCCGGAAACCCTGCTGGGCGACGCCGCGGTCGCGGTGCACCCGGAAGACGAGCGCTACGCC
AAGCTGATCGGCCAGTTCGCCGAACTGCCGATCGTCGGCCGGCGCATCCCGATCGTCGCCGACGAGTACGTC
GACCGCGAATTCGGCACCGGCTGCGTGAAGATCACCCCGGCCCACGACTTCAACGACTACGAAGTGGGCAAG
CGCCACGACCTGCCGCTGATCAACATCTTCGACAAGAACGCCGCGGTGCTGGCCCAGGCCCAGGTGTTCCAC
CTCGACGGCAGCGTCAACCCGAACCTCGACCCCAGCCTGCCGCAGAGCTACGCCGGCATGGACCGCTTCGCC
GCGCGCAAGGCCATCGTCGCCGAGTTCGAGGCCATGGGCCTGCTCGAGAAGGTCGACGACCACGCACTGAAA
GTACCGAAGGGCGACCGCTCCGGCACCGTCATCGAACCCTGGCTGACCGACCAGTGGTACGTCAGCACCAAG
CCGCTGGCCGAAGACGCCATCGCCGCCGTGGAAGACGGCCGTATCCAGTTCGTGCCCAAGCAGTACGAGAAC
ATGTACTTCTCCTGGATGCGCGACATCCAGGACTGGTGCATCAGCCGCCAGCTCTGGTGGGGCCACCGCATC
CCGGCCTGGTACGACGAGGCCGGCAACGTCTACGTCGGCCGCGACGAGGTCGAGGTGCGCACCAAGCACAAG
CTCGGCAACGAGGTCGAGTTGCGCCAGGACGAAGACGTTCTCGACACCTGGTTCAGCTCCGGCCTGTGGACC
TTCTCCACCCTCGGCTGGCCGCAGCAGACCGAGTTCCTCAAGACCTTCCACCCCACCGACGTGCTGGTCACC
GGCTTCGACATCATCTTCTTCTGGGTCGCGCGGATGATCATGCTCACCATGCACCTGGTGAAGAACCCCGAC
GGCACCCCGCAGATCCCGTTCAAGACCGTCTATGTCCACGGCCTGGTGCGCGACGGCCAGGGCCAGAAGATG
TCCAAGTCCAAGGGCAACGTACTCGACCCGCTGGACATCGTCGACGGCATCGACCTCGATACCCTGCTGCAG
AAACGCACCAGCGGCATGATGCAGCCGAAGCTGGCGGAGAAGATCGCCAAGCAGACCCGCGCCGAATTCCCC
GAAGGCATCGCCAGCTACGGCACCGACGCCCTGCGCTTCACCTTCTGCTCGCTGGCCTCCACCGGCCGCGAC
ATCAAGTTCGACATGGGCCGCGTCGAAGGCTTCCGCAACTTCTGCAACAAGATCTGGAACGCCGCCAACTTC
GTCATCGAGAACACCGATGGCCAGGACACCGGGGTCAACGGCGAGCCGGTCGAGCTGTCCTCGGTCGACCGC
TGGATCATCTCCCAGTTGCAGCGCACCGAGCAGGAAGTCACCCGCCAGCTCGACGCCTTCCGCTTCGACCTG
GCCGCCCAGGCCCTCTATGAGTTCATCTGGGACGAGTACTGCGCCTGGTACCTGGAGCTGGTCAAGCCGGTG
CTGTGGGACGAGAACGCCCCCATCGAGCGCCAGCGCGGCACTCGTCGCACCCTGATCCGCGTGCTGGAGACG
GCCCTGCGCCTGGCGCACCCGTTCATGCCGTTCATCACCGAGGAAATCTGGCAGCGCATCAAGGGCCAGGCC
GGCAAGGAAGGTCCGACCCTGATGCTGCAACCCTGGCCGGTGGCCGACGAAGGCAGGATCGACGCCGCCGCC
GAAGGCGACATCGAATGGGTCAAGGCGCTGATGCTCGGCGTACGGCAGATCCGCGGCGAGATGAACATCTCC
ATGGCCAAGCGCATCGACATCATCCTCAGGAACGCCTCGCCCAGCGACCACCGCCGCCTGGCCGACAACGAG
CCGCTGCTGATGAAACTGGCCAAGCTGGAAAGCATCCGCGTGCTGGAAGCCGGCGAGGAAGCGCCGATGTCC
GCCACCGCCCTGGTCGGCGACATGGAAGTATTGGTGCCGATGGCCGGCCTGATCGACAAGAGCGCCGAACTG
GGGCGCCTGGACAAGGAGATCCAGCGCCTGGAAGGCGAGGTCAAGCGCGTCGGCGGCAAGCTGTCCAACGAA
GGCTTCGTCGCCAAGGCCCCGGCCGACGTGATCGAGAAGGAACGCGCCAAGCTAGCCGAGGCCGAACAGGCC
CTGGCCAAGCTGGCCGAGCAACGGCAGAAGATCGCCGCGCTCTAA
Translation:
MDKTYQPHAIETSWYETWESNDYFAPSGEGQPYTIMIPPPNVTGSLHMGHGFNNAIMDALIRYRRMQGRNTL
WQPGTDHAGIATQMVVERQLGAQGVSRHDLGREKFLEKVWEWKEQSGGNITRQIRRLGSSVDWSRERFTMDD
GLSEAVKEAFVRLHEDGLIYRGKRLVNWDTKLHTAISDLEVENHDEKGHLWHLRYPLVKGAKTSEGLDYLVV
ATTRPETLLGDAAVAVHPEDERYAKLIGQFAELPIVGRRIPIVADEYVDREFGTGCVKITPAHDFNDYEVGK
RHDLPLINIFDKNAAVLAQAQVFHLDGSVNPNLDPSLPQSYAGMDRFAARKAIVAEFEAMGLLEKVDDHALK
VPKGDRSGTVIEPWLTDQWYVSTKPLAEDAIAAVEDGRIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRI
PAWYDEAGNVYVGRDEVEVRTKHKLGNEVELRQDEDVLDTWFSSGLWTFSTLGWPQQTEFLKTFHPTDVLVT
GFDIIFFWVARMIMLTMHLVKNPDGTPQIPFKTVYVHGLVRDGQGQKMSKSKGNVLDPLDIVDGIDLDTLLQ
KRTSGMMQPKLAEKIAKQTRAEFPEGIASYGTDALRFTFCSLASTGRDIKFDMGRVEGFRNFCNKIWNAANF
VIENTDGQDTGVNGEPVELSSVDRWIISQLQRTEQEVTRQLDAFRFDLAAQALYEFIWDEYCAWYLELVKPV
LWDENAPIERQRGTRRTLIRVLETALRLAHPFMPFITEEIWQRIKGQAGKEGPTLMLQPWPVADEGRIDAAA
EGDIEWVKALMLGVRQIRGEMNISMAKRIDIILRNASPSDHRRLADNEPLLMKLAKLESIRVLEAGEEAPMS
ATALVGDMEVLVPMAGLIDKSAELGRLDKEIQRLEGEVKRVGGKLSNEGFVAKAPADVIEKERAKLAEAEQA
LAKLAEQRQKIAAL*
AnnotationID:90GeneID:80
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 22:10:17
Gene Name:valS
Confidence Code:2
GeneProduct: valyl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:KOG0432,COG0525,pfam00133
Secondary Functional Category(ies):
EC Number:6.1.1.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66602.1|AE016288_1 (AE016777) valyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 948

 Score = 1626 bits (4211), Expect = 0.0
 Identities = 788/950 (82%), Positives = 853/950 (89%), Gaps = 2/950 (0%)

>gb|AAG59457.1|AE005657_17 (AE005657) valine tRNA synthetase [Escherichia coli O157:H7 EDL933]
          Length = 951

 Score = 1238 bits (3204), Expect = 0.0
 Identities = 599/954 (62%), Positives = 730/954 (76%), Gaps = 7/954 (0%)








 
Structural Features:
KOG0432, Valyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 995 residues,  95.8% aligned
Score =  987 bits (2552), Expect = 0.0
Identities = 438/985 (44%), Positives = 594/985 (60%), Gaps = 67/985 (6%)

COG0525, ValS, Valyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 877 residues, 100.0% aligned
Score = 1238 bits (3205), Expect = 0.0
Identities = 518/953 (54%), Positives = 639/953 (67%), Gaps = 80/953 (8%)

pfam00133, tRNA-synt_1, tRNA synthetases class I (I, L, M and V).
CD-Length = 603 residues,  99.8% aligned
Score =  606 bits (1563), Expect = 1e-173
Identities = 252/666 (37%), Positives = 334/666 (50%), Gaps = 115/666 (17%)






   
Genomic Context:






   
Comment:


















    ReferenceID:163




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17305




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31724




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:80_cdd.html


    		




 
URL:

















    ReferenceID:34320




  
Author/Investigator(s):
        Hartlein M, Frank R, Madern D.





  
Title:
        Nucleotide sequence of Escherichia coli valyl-tRNA synthetase gene valS.




  
PubMed:
        3317277


MedLine:
        




    
Source:
Nucleotide sequence of Escherichia coli valyl-tRNA synthetase gene valS.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3317277

















    ReferenceID:34321




  
Author/Investigator(s):
        Taylor BV, Toy J, Sit TL, Bognar AL.





  
Title:
        Cloning and sequence determination of the valS gene, encoding valyl-tRNA synthetase in Lactobacillus casei.




  
PubMed:
        8468307


MedLine:
        




    
Source:
J Bacteriol. 1993 Apr;175(8):2475-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8468307









           
Homologs By Global Alignment 

           

       



     
Gene ID:80
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
157
                           PA3834_tr
                           translation of PA3834
                           950
                           99.47
                           99.68
                           0
                           4986.0
     

     
     
13663
                           gb|AAG07221.1|AE004800_10
                           (AE004800) valyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           950
                           99.47
                           99.68
                           0
                           4986.0
     

     
     
13664
                           gb|AAN66602.1|AE016288_1
                           (AE016777) valyl-tRNA synthetase [Pseudomonas putida KT2440]
                           950
                           82.94
                           89.78
                           2
                           4213.0
     

     
     
13665
                           gb|AAO54793.1|
                           (AE016860) valyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           950
                           82.10
                           89.89
                           2
                           4185.0
     

     
     
13671
                           dbj|BAC95674.1|
                           (AP005341) valyl-tRNA synthetase [Vibrio vulnificus YJ016]
                           954
                           64.67
                           76.62
                           7
                           3283.0
     

     






      

          
GC ORFID: 43082How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3834Glimmer Score: 

          
Start: 3719505Stop: 3722357

          
Length: 2853


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3834 translation of PA3834Homolog Bit Score 1925.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4974 (ORF: BLASTX 410.0), t_PA3834 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGACAAGACTTACCAGCCGCACGCCATCGAGACTTCCTGGTACGAGACCTGGGAGTCCAACGACTACTTC 
GCTCCGAGCGGCGAAGGCCAGCCCTACACCATCATGATCCCGCCGCCGAACGTGACCGGCAGCCTGCACATG 
GGCCATGGCTTCAACAACGCGATAATGGACGCGCTGATCCGCTACCGCCGCATGCAGGGCCGCAACACCCTG 
TGGCAGCCGGGCACCGATCACGCCGGCATCGCCACCCAGATGGTGGTGGAGCGCCAGCTCGGCGCCCAGGGC 
GTGAGCCGCCACGACCTCGGCCGGGAGAAGTTCCTGGAGAAGGTCTGGGAATGGAAGGAACAGTCCGGCGGC 
AACATCACCCGGCAGATTCGTCGCCTGGGCTCCTCGGTGGACTGGTCGCGCGAGCGCTTCACCATGGACGAC 
GGCCTCTCCGAAGCGGTCAAGGAAGCCTTCGTGCGCCTGCATGAAGACGGCCTGATCTACCGCGGCAAACGC 
CTGGTGAACTGGGACACCAAGCTGCACACCGCGATTTCCGACCTGGAAGTGGAAAACCACGACGAGAAGGGC 
CACCTCTGGCACCTGCGCTACCCGCTGGTGAAAGGCGCGAAGACCAGCGAAGGCCTGGACTACCTGGTGGTC 
GCCACTACCCGTCCGGAAACCCTGCTGGGCGACGCCGCGGTCGCGGTGCACCCGGAAGACGAGCGCTACGCC 
AAGCTGATCGGCCAGTTCGCCGAACTGCCGATCGTCGGCCGGCGCATCCCGATCGTCGCCGACGAGTACGTC 
GACCGCGAATTCGGCACCGGCTGCGTGAAGATCACCCCGGCCCACGACTTCAACGACTACGAAGTGGGCAAG 
CGCCACGACCTGCCGCTGATCAACATCTTCGACAAGAACGCCGCGGTGCTGGCCCAGGCCCAGGTGTTCCAC 
CTCGACGGCAGCGTCAACCCGAACCTCGACCCCAGCCTGCCGCAGAGCTACGCCGGCATGGACCGCTTCGCC 
GCGCGCAAGGCCATCGTCGCCGAGTTCGAGGCCATGGGCCTGCTCGAGAAGGTCGACGACCACGCACTGAAA 
GTACCGAAGGGCGACCGCTCCGGCACCGTCATCGAACCCTGGCTGACCGACCAGTGGTACGTCAGCACCAAG 
CCGCTGGCCGAAGACGCCATCGCCGCCGTGGAAGACGGCCGTATCCAGTTCGTGCCCAAGCAGTACGAGAAC 
ATGTACTTCTCCTGGATGCGCGACATCCAGGACTGGTGCATCAGCCGCCAGCTCTGGTGGGGCCACCGCATC 
CCGGCCTGGTACGACGAGGCCGGCAACGTCTACGTCGGCCGCGACGAGGTCGAGGTGCGCACCAAGCACAAG 
CTCGGCAACGAGGTCGAGTTGCGCCAGGACGAAGACGTTCTCGACACCTGGTTCAGCTCCGGCCTGTGGACC 
TTCTCCACCCTCGGCTGGCCGCAGCAGACCGAGTTCCTCAAGACCTTCCACCCCACCGACGTGCTGGTCACC 
GGCTTCGACATCATCTTCTTCTGGGTCGCGCGGATGATCATGCTCACCATGCACCTGGTGAAGAACCCCGAC 
GGCACCCCGCAGATCCCGTTCAAGACCGTCTATGTCCACGGCCTGGTGCGCGACGGCCAGGGCCAGAAGATG 
TCCAAGTCCAAGGGCAACGTACTCGACCCGCTGGACATCGTCGACGGCATCGACCTCGATACCCTGCTGCAG 
AAACGCACCAGCGGCATGATGCAGCCGAAGCTGGCGGAGAAGATCGCCAAGCAGACCCGCGCCGAATTCCCC 
GAAGGCATCGCCAGCTACGGCACCGACGCCCTGCGCTTCACCTTCTGCTCGCTGGCCTCCACCGGCCGCGAC 
ATCAAGTTCGACATGGGCCGCGTCGAAGGCTTCCGCAACTTCTGCAACAAGATCTGGAACGCCGCCAACTTC 
GTCATCGAGAACACCGATGGCCAGGACACCGGGGTCAACGGCGAGCCGGTCGAGCTGTCCTCGGTCGACCGC 
TGGATCATCTCCCAGTTGCAGCGCACCGAGCAGGAAGTCACCCGCCAGCTCGACGCCTTCCGCTTCGACCTG 
GCCGCCCAGGCCCTCTATGAGTTCATCTGGGACGAGTACTGCGCCTGGTACCTGGAGCTGGTCAAGCCGGTG 
CTGTGGGACGAGAACGCCCCCATCGAGCGCCAGCGCGGCACTCGTCGCACCCTGATCCGCGTGCTGGAGACG 
GCCCTGCGCCTGGCGCACCCGTTCATGCCGTTCATCACCGAGGAAATCTGGCAGCGCATCAAGGGCCAGGCC 
GGCAAGGAAGGTCCGACCCTGATGCTGCAACCCTGGCCGGTGGCCGACGAAGGCAGGATCGACGCCGCCGCC 
GAAGGCGACATCGAATGGGTCAAGGCGCTGATGCTCGGCGTACGGCAGATCCGCGGCGAGATGAACATCTCC 
ATGGCCAAGCGCATCGACATCATCCTCAGGAACGCCTCGCCCAGCGACCACCGCCGCCTGGCCGACAACGAG 
CCGCTGCTGATGAAACTGGCCAAGCTGGAAAGCATCCGCGTGCTGGAAGCCGGCGAGGAAGCGCCGATGTCC 
GCCACCGCCCTGGTCGGCGACATGGAAGTATTGGTGCCGATGGCCGGCCTGATCGACAAGAGCGCCGAACTG 
GGGCGCCTGGACAAGGAGATCCAGCGCCTGGAAGGCGAGGTCAAGCGCGTCGGCGGCAAGCTGTCCAACGAA 
GGCTTCGTCGCCAAGGCCCCGGCCGACGTGATCGAGAAGGAACGCGCCAAGCTAGCCGAGGCCGAACAGGCC 
CTGGCCAAGCTGGCCGAGCAACGGCAGAAGATCGCCGCGCTCTAA