ViewGene
Locus Name:
GeneID:
Location:

GeneID:79
TrivialName: PA14_66270
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66270
PAO1 Ortholog Locus:PA5014
Sequence Length:2949
Protein Length:982
Start: 5907271
Stop: 5904323
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08399.1|AE004913_14 (AE004913) glutamate-ammonia-ligase adenylyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 978/982 (99%), Positives = 979/982 (99%)
Sequence:
ATGAGCCTGCCTTCGCTGGCGAATCTGCCGGCCACCCTGCTGCCCGCCGCCGAACGCGCCGGGACTGCCCTC
CGCTCCGCTGTCGCCGCCCTCGATGCCGCCGCCCTGGCCCGCCTGGAGGCCTGGCCCGAGGAGCGCCTGGAG
GATTTCCGACGGGTCGCTGCGGCCAGCGATTTCGTCGCCGAGCAGGCAGTGCGCGACCCGGCGATGCTGCTG
GATCTGGCCGAACGCGGCGAGCTGGAGAACCCCCACGCGCCCGGCGAATTGCGCAGCCAGTTGCAGGCGCGG
CTGGAGGATTGCGCCGACGAGGACGAACTCGGTCGGCGCCTGCGACGTTTCCGCACACGCCAGCAACTGCGC
ATCATCTGGCGCGACCTGACCCGCCGCGCCGCGCTGGCGGAAACCTGTCGCGACCTGTCGGGCCTGGCCGAC
GCCTGCATCGACCTGGCTTGCGAGTGGCTCCATCGGCGCCAGTGCGAGCAGTTCGGCACGCCGATCGGCCGG
CGCTCCGGCGAACCGCAGCGGATGGTGGTCCTCGGCATGGGCAAGCTGGGCGCGGTGGAGCTCAACCTGTCG
TCGGACATCGACCTGATCTTCGGTTACCCGGAGGGCGGCGAGACCGAGGGGGCGAAGCGTTCGCTGGACAAC
CAGGAGTTCTTCACCCGCCTGGGGCAGAAGCTGATCAAGGCGCTGGATGCGATCACCGTCGACGGCTTCGTC
TTCCGCGTCGACATGCGCCTGCGTCCCTACGGCTCCAGCGGACCGCTGGTCTACAGCTTCGCCGCGCTGGAG
CAGTACTACCAGGACCAGGGGCGCGACTGGGAGCGCTACGCGATGATCAAGGCGCGGGTGGTCGGCGGCGAC
CAGCAGGCAGGCGAGCAACTGCTCGGCATGCTGCGGCCGTTCGTCTATCGGCGCTACCTGGACTTCTCCGCC
ATCGAGGCGCTGCGCACCATGAAGCAGCTGATCCAGCAGGAGGTCCGGCGCAAGGGTATGTCGGAAAACATC
AAGCTGGGCGAAGGCGGTATCCGCGAGGTGGAGTTCATCGCCCAGGCCTTCCAGCTGATCCATGGCGGGCGC
GACCTGAGCTTGCAGCAACGGCCGCTGCTGAAGGTGCTGGCGACCCTGGAGGGGCAGGGCTACCTGCCGCCG
GCGGTGGTCGAGGAGCTGCGCGGCGGCTACGAGTTCCTGCGCTACGCCGAGCATGCCATCCAGGCGCTGGCC
GACCGGCAGACGCAAATGCTGCCCAGCGACGAATACGACCGCATCCGCGTGGCCTTCATCATGGGCTTCGCC
AGTTGGGCCGCATTCCACGAGCGCCTGAGCCACTGGCGGGCGCGCATCGACTGGCACTTCCGCCAGGTGATC
GCCGACCCGGACGAGGACGAAAGCGGCGAGGCGGCCTGCGGCAGCGTCGGCGCCGAGTGGATCCCGCTGTGG
GAGGAGGCGCTGGACGAGGAGTCGGCCAGTCGGCAACTGGCCGACGCCGGCTTCGTCGATGCCGAGGCGGCC
TGGAAGCGTCTCAGCGATCTGCGCCACGGTCCCCAGGTACGAGCGATGCAGCGTCTCGGCCGGGAACGCCTG
GATGCCTTCGTTCCGCGCCTGCTGGCGATGACCGTGGAAAACCCCCAGCCGGACCTGGTGCTGGAGCGGGTT
CTGCCGCTGGTGGAAGCGGTGGCGCGGCGTTCCGCCTACCTGGTGTTGCTGACCGAGAACCCCGGCGCACTG
GAGCGCCTGCTGACCCTCTGCGCGGCCAGCCCGATGGTTGCCGAGCAGATCGCGCGGTTCCCGATCCTGCTC
GACGAACTGCTCAACGAGGGGCGCCTGTTCCGTCCTCCACAGGCCGCCGAGCTGGCCGCCGAGCTGCGCGAA
CGGCTGATGCGGATTCCCGAGGACGACCTCGAGCAGCAGATGGAGACCCTTCGTCACTTCAAGCTGGCCCAC
GGGCTGCGGGTGGCGGCCTCGGAGATCGCCGGCACGCTGCCGTTGATGAAGGTCAGCGACTACCTGACCTGG
CTGGCCGAGGCGATCCTCGTCGAGGTCCTCGAACTGGCCTGGCGGCAGTTGGTGCAGCGTCACGGCCGGCCG
TTGCGCGCCGACGGCACGCCGTGCGATCCGGACTTCGTCATCGTCGGCTACGGCAAGGTCGGCGGGCTGGAG
TTCGGTCATGGCTCGGACCTCGACCTGGTGTTTATCCATGACGGCGACCCCCACTGCGAGACCGATGGCGGC
AAATCCATCGATGGCGCGCAGTTCTTTACCCGCCTGGGGCAGAAGATCATCCATTTCCTCACCGCGCAGACG
CCATCCGGCACCCTCTACGAGGTCGACATGCGCCTGCGTCCGAGCGGCGCGGCCGGCCTGCTGGTGAGTTCG
CTGGGCGCCTTCCAGCGCTACCAGGAGCAGGAGGCCTGGACCTGGGAGCACCAGGCGTTGGTCCGCGCCCGG
GTGCTGGCCGGTTGCCGGCGGGTGCAGGCGAGCTTCGAGGCGGTACGTGCCGAGGTGCTGGCGAGGCCGCGC
GATCTCGACGCGTTGCGCACCGAAGTCAGCGAAATGCGCGCCAAGATGCGCGACAACCTGGGAACCCGCGCG
ACCGCCGCCGGCACCGCCTCGAATGCCTTCGAGGCCACGGCAGCCTTCGATCTCAAGCACGATGCCGGTGGT
ATCGTCGATATCGAATTTATGGTGCAATATGCGGTTCTAGCTTGGTCCGGAGAACACCCGGCGCTGCTCGAA
TTCACCGATAACATCCGCATTCTGGAAGGACTGGAACGGGCTGGCCTGATCGCCAGCGAGGACGTCCGTCTC
CTGCAGGAGGCCTACAAGGCCTATCGCGCAGCCGCACACCGCCTGGCACTACAGAAGGAGGCCGGGGTCGTG
AGCGGCGAGCACTTCCAGACGGAGCGGCGAGAAGTGATCAGGATCTGGCGCGAGCTGCGGCTCGGCTGA
Translation:
MSLPSLANLPATLLPAAERAGTALRSAVAALDAAALARLEAWPEERLEDFRRVAAASDFVAEQAVRDPAMLL
DLAERGELENPHAPGELRSQLQARLEDCADEDELGRRLRRFRTRQQLRIIWRDLTRRAALAETCRDLSGLAD
ACIDLACEWLHRRQCEQFGTPIGRRSGEPQRMVVLGMGKLGAVELNLSSDIDLIFGYPEGGETEGAKRSLDN
QEFFTRLGQKLIKALDAITVDGFVFRVDMRLRPYGSSGPLVYSFAALEQYYQDQGRDWERYAMIKARVVGGD
QQAGEQLLGMLRPFVYRRYLDFSAIEALRTMKQLIQQEVRRKGMSENIKLGEGGIREVEFIAQAFQLIHGGR
DLSLQQRPLLKVLATLEGQGYLPPAVVEELRGGYEFLRYAEHAIQALADRQTQMLPSDEYDRIRVAFIMGFA
SWAAFHERLSHWRARIDWHFRQVIADPDEDESGEAACGSVGAEWIPLWEEALDEESASRQLADAGFVDAEAA
WKRLSDLRHGPQVRAMQRLGRERLDAFVPRLLAMTVENPQPDLVLERVLPLVEAVARRSAYLVLLTENPGAL
ERLLTLCAASPMVAEQIARFPILLDELLNEGRLFRPPQAAELAAELRERLMRIPEDDLEQQMETLRHFKLAH
GLRVAASEIAGTLPLMKVSDYLTWLAEAILVEVLELAWRQLVQRHGRPLRADGTPCDPDFVIVGYGKVGGLE
FGHGSDLDLVFIHDGDPHCETDGGKSIDGAQFFTRLGQKIIHFLTAQTPSGTLYEVDMRLRPSGAAGLLVSS
LGAFQRYQEQEAWTWEHQALVRARVLAGCRRVQASFEAVRAEVLARPRDLDALRTEVSEMRAKMRDNLGTRA
TAAGTASNAFEATAAFDLKHDAGGIVDIEFMVQYAVLAWSGEHPALLEFTDNIRILEGLERAGLIASEDVRL
LQEAYKAYRAAAHRLALQKEAGVVSGEHFQTERREVIRIWRELRLG*
AnnotationID:89GeneID:79
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 09:09:15
Gene Name:glnE
Confidence Code:2
GeneProduct:glutamate-ammonia-ligase adenylyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1391,pfam03710
Secondary Functional Category(ies):signal transduction mechanism
EC Number:2.7.7.42
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58432.1| (AE016874) glutamate-ammonia-ligase adenylyltransferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 985

 Score = 1459 bits (3777), Expect = 0.0
 Identities = 748/984 (76%), Positives = 810/984 (82%), Gaps = 3/984 (0%)

>gb|AAN65971.1|AE016225_4 (AE016775) glutamate-ammonia-ligase adenylyltransferase
           [Pseudomonas putida KT2440]
          Length = 977

 Score = 1435 bits (3715), Expect = 0.0
 Identities = 736/984 (74%), Positives = 815/984 (82%), Gaps = 11/984 (1%)

>gb|AAF95580.1| (AE004313) glutamate-ammonia-ligase adenylyltransferase [Vibrio
           cholerae]
          Length = 948

 Score =  805 bits (2079), Expect = 0.0
 Identities = 454/976 (46%), Positives = 598/976 (61%), Gaps = 37/976 (3%)








 
Structural Features:
COG1391, GlnE, Glutamine synthetase adenylyltransferase 
[Posttranslational modification, protein turnover, chaperones / 
Signal transduction mechanisms]
CD-Length = 963 residues,  99.3% aligned
Score = 1046 bits (2707), Expect = 0.0
Identities = 510/987 (51%), Positives = 617/987 (62%), Gaps = 36/987 (3%)

pfam03710, GlnE, Glutamate-ammonia ligase adenylyltransferase.
CD-Length = 222 residues, 100.0% aligned
Score =  269 bits (689), Expect = 1e-72
Identities = 113/223 (50%), Positives = 149/223 (66%), Gaps = 3/223 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:161




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17303




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35077




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:79_cdd.html


    		




 
URL:

















    ReferenceID:35868




  
Author/Investigator(s):
        van Heeswijk WC, Rabenberg M, Westerhoff HV, Kahn D.





  
Title:
        The genes of the glutamine synthetase adenylylation cascade are not regulated by nitrogen in Escherichia coli.




  
PubMed:
        8412694


MedLine:
        




    
Source:
Mol Microbiol. 1993 Aug;9(3):443-57.






    
Reference Type: 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=94018640&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:79
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
156
                           PA5014_tr
                           translation of PA5014
                           982
                           99.59
                           99.69
                           0
                           4991.0
     

     
     
13633
                           gb|AAG08399.1|AE004913_14
                           (AE004913) glutamate-ammonia-ligase adenylyltransferase [Pseudomonas aeruginosa PAO1]
                           982
                           99.59
                           99.69
                           0
                           4991.0
     

     
     
13634
                           gb|AAO58432.1|
                           (AE016874) glutamate-ammonia-ligase adenylyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           985
                           75.93
                           82.23
                           3
                           3780.0
     

     
     
13635
                           gb|AAN65971.1|AE016225_4
                           (AE016775) glutamate-ammonia-ligase adenylyltransferase [Pseudomonas putida KT2440]
                           985
                           74.72
                           82.74
                           11
                           3726.0
     

     
     
13636
                           gb|AAC33171.1|
                           (U63816) unknown [Pseudomonas aeruginosa]
                           982
                           51.42
                           51.42
                           476
                           2563.0
     

     






      

          
GC ORFID: 43081How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5014Glimmer Score: 

          
Start: 5170954Stop: 5168006

          
Length: 2949


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5014 translation of PA5014Homolog Bit Score 1927.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3377 (ORF: BLASTX 1472.0), t_PA5014 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCTGCCTTCGCTGGCGAATCTGCCGGCCACCCTGCTGCCCGCCGCCGAACGCGCCGGGACTGCCCTC 
CGCTCCGCTGTCGCCGCCCTCGATGCCGCCGCCCTGGCCCGCCTGGAGGCCTGGCCCGAGGAGCGCCTGGAG 
GATTTCCGACGGGTCGCTGCGGCCAGCGATTTCGTCGCCGAGCAGGCAGTGCGCGACCCGGCGATGCTGCTG 
GATCTGGCCGAACGCGGCGAGCTGGAGAACCCCCACGCGCCCGGCGAATTGCGCAGCCAGTTGCAGGCGCGG 
CTGGAGGATTGCGCCGACGAGGACGAACTCGGTCGGCGCCTGCGACGTTTCCGCACACGCCAGCAACTGCGC 
ATCATCTGGCGCGACCTGACCCGCCGCGCCGCGCTGGCGGAAACCTGTCGCGACCTGTCGGGCCTGGCCGAC 
GCCTGCATCGACCTGGCTTGCGAGTGGCTCCATCGGCGCCAGTGCGAGCAGTTCGGCACGCCGATCGGCCGG 
CGCTCCGGCGAACCGCAGCGGATGGTGGTCCTCGGCATGGGCAAGCTGGGCGCGGTGGAGCTCAACCTGTCG 
TCGGACATCGACCTGATCTTCGGTTACCCGGAGGGCGGCGAGACCGAGGGGGCGAAGCGTTCGCTGGACAAC 
CAGGAGTTCTTCACCCGCCTGGGGCAGAAGCTGATCAAGGCGCTGGATGCGATCACCGTCGACGGCTTCGTC 
TTCCGCGTCGACATGCGCCTGCGTCCCTACGGCTCCAGCGGACCGCTGGTCTACAGCTTCGCCGCGCTGGAG 
CAGTACTACCAGGACCAGGGGCGCGACTGGGAGCGCTACGCGATGATCAAGGCGCGGGTGGTCGGCGGCGAC 
CAGCAGGCAGGCGAGCAACTGCTCGGCATGCTGCGGCCGTTCGTCTATCGGCGCTACCTGGACTTCTCCGCC 
ATCGAGGCGCTGCGCACCATGAAGCAGCTGATCCAGCAGGAGGTCCGGCGCAAGGGTATGTCGGAAAACATC 
AAGCTGGGCGAAGGCGGTATCCGCGAGGTGGAGTTCATCGCCCAGGCCTTCCAGCTGATCCATGGCGGGCGC 
GACCTGAGCTTGCAGCAACGGCCGCTGCTGAAGGTGCTGGCGACCCTGGAGGGGCAGGGCTACCTGCCGCCG 
GCGGTGGTCGAGGAGCTGCGCGGCGGCTACGAGTTCCTGCGCTACGCCGAGCATGCCATCCAGGCGCTGGCC 
GACCGGCAGACGCAAATGCTGCCCAGCGACGAATACGACCGCATCCGCGTGGCCTTCATCATGGGCTTCGCC 
AGTTGGGCCGCATTCCACGAGCGCCTGAGCCACTGGCGGGCGCGCATCGACTGGCACTTCCGCCAGGTGATC 
GCCGACCCGGACGAGGACGAAAGCGGCGAGGCGGCCTGCGGCAGCGTCGGCGCCGAGTGGATCCCGCTGTGG 
GAGGAGGCGCTGGACGAGGAGTCGGCCAGTCGGCAACTGGCCGACGCCGGCTTCGTCGATGCCGAGGCGGCC 
TGGAAGCGTCTCAGCGATCTGCGCCACGGTCCCCAGGTACGAGCGATGCAGCGTCTCGGCCGGGAACGCCTG 
GATGCCTTCGTTCCGCGCCTGCTGGCGATGACCGTGGAAAACCCCCAGCCGGACCTGGTGCTGGAGCGGGTT 
CTGCCGCTGGTGGAAGCGGTGGCGCGGCGTTCCGCCTACCTGGTGTTGCTGACCGAGAACCCCGGCGCACTG 
GAGCGCCTGCTGACCCTCTGCGCGGCCAGCCCGATGGTTGCCGAGCAGATCGCGCGGTTCCCGATCCTGCTC 
GACGAACTGCTCAACGAGGGGCGCCTGTTCCGTCCTCCACAGGCCGCCGAGCTGGCCGCCGAGCTGCGCGAA 
CGGCTGATGCGGATTCCCGAGGACGACCTCGAGCAGCAGATGGAGACCCTTCGTCACTTCAAGCTGGCCCAC 
GGGCTGCGGGTGGCGGCCTCGGAGATCGCCGGCACGCTGCCGTTGATGAAGGTCAGCGACTACCTGACCTGG 
CTGGCCGAGGCGATCCTCGTCGAGGTCCTCGAACTGGCCTGGCGGCAGTTGGTGCAGCGTCACGGCCGGCCG 
TTGCGCGCCGACGGCACGCCGTGCGATCCGGACTTCGTCATCGTCGGCTACGGCAAGGTCGGCGGGCTGGAG 
TTCGGTCATGGCTCGGACCTCGACCTGGTGTTTATCCATGACGGCGACCCCCACTGCGAGACCGATGGCGGC 
AAATCCATCGATGGCGCGCAGTTCTTTACCCGCCTGGGGCAGAAGATCATCCATTTCCTCACCGCGCAGACG 
CCATCCGGCACCCTCTACGAGGTCGACATGCGCCTGCGTCCGAGCGGCGCGGCCGGCCTGCTGGTGAGTTCG 
CTGGGCGCCTTCCAGCGCTACCAGGAGCAGGAGGCCTGGACCTGGGAGCACCAGGCGTTGGTCCGCGCCCGG 
GTGCTGGCCGGTTGCCGGCGGGTGCAGGCGAGCTTCGAGGCGGTACGTGCCGAGGTGCTGGCGAGGCCGCGC 
GATCTCGACGCGTTGCGCACCGAAGTCAGCGAAATGCGCGCCAAGATGCGCGACAACCTGGGAACCCGCGCG 
ACCGCCGCCGGCACCGCCTCGAATGCCTTCGAGGCCACGGCAGCCTTCGATCTCAAGCACGATGCCGGTGGT 
ATCGTCGATATCGAATTTATGGTGCAATATGCGGTTCTAGCTTGGTCCGGAGAACACCCGGCGCTGCTCGAA 
TTCACCGATAACATCCGCATTCTGGAAGGACTGGAACGGGCTGGCCTGATCGCCAGCGAGGACGTCCGTCTC 
CTGCAGGAGGCCTACAAGGCCTATCGCGCAGCCGCACACCGCCTGGCACTACAGAAGGAGGCCGGGGTCGTG 
AGCGGCGAGCACTTCCAGACGGAGCGGCGAGAAGTGATCAGGATCTGGCGCGAGCTGCGGCTCGGCTGA