ViewGene
Locus Name:
GeneID:
Location:

GeneID:783
TrivialName: PA14_16530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16530
PAO1 Ortholog Locus:PA3700
Sequence Length:1506
Protein Length:501
Start: 1415600
Stop: 1417105
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07088.1|AE004789_8 (AE004789) lysyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 499/501 (99%), Positives = 501/501 (100%)
Sequence:
ATGAGCGACCAACAACTCGACCAGCACGAACTGCAACAGGAAGAAAACAAGCTGATCGCCCAGCGCAAGGAA
AAACTTGCCGCCGTGCGCGAAGCCCGGGCCATCGCCTTCCCCAACGACTTCCGCCGCGACGCCTACTTCGCG
GACCTGCAGAAACAGTACGCCGACAAGACCAAGGAAGAGCTGGAAGCCGCAGCGATCCCGGTCAAGGTGGCC
GGTCGGATCATGCTCAACCGCGGTTCCTTCATCGTCCTCCAGGACAGCAGCGAGCGCCTGCAGGTCTACGTC
AACCGCAAGACCCTGCCGGAAGAAACCCTGGCCGAGATCAAGACCTGGGACCTGGGCGACATCATCGGCGCC
GAAGGCGTGCTGGCCCGCTCGGGCAAGGGCGACCTGTACGTCGACATGACCAGCGTGCGCCTGCTGACCAAG
TCGCTGCGTCCGCTGCCGGACAAGCACCATGGCCTGACCGACACCGAGCAGCGCTACCGCCAGCGCTACGTC
GACCTGATGGTCAACGAGGAAACCCGCCACACCTTCCGCGTGCGCTCCCAGGTCATCGCCCACATCCGCCGT
TTCCTCAGCGAGCGCGGTTTCCTCGAAGTGGAGACCCCGATGCTGCAGACCATCCCCGGCGGTGCCGCGGCC
AAGCCGTTCGAGACCCATCACAACGCGCTGGACATGGCCATGTTCCTGCGCATCGCCCCGGAGCTGTACCTC
AAGCGCCTGGTGGTCGGCGGTTTCGAGAAGGTCTTCGAGATCAACCGCAACTTCCGTAACGAAGGCGTCTCG
ACCCGGCACAACCCCGAGTTCACCATGCTCGAGTTCTACCAGGCCTACGCCGACTACGAAGACAACATGGAC
CTGACCGAGGAACTGTTCCGCGAACTGGCCCAGGCGGTACTCGGTACCACCGACGTGCCCTACGGCGACAAG
GTCTTCCACTTCGGCGAGCCGTTCGTGCGCCTGTCGGTGTTCGATTCGATCCTCAAGTACAACCCGGAGATC
AGCGCCGCCGACCTCAACGACGTCGAGAAGGCGCGGGCCATCGCCAAGAAGGCCGGCGCCAAGGTCCTCGGC
CACGAAGGCCTGGGCAAGCTGCAGGTGATGATTTTCGAGGAACTGGTGGAGCACAAGCTGGAGCAGCCGCAC
TTCATCACCCGCTACCCCTTCGAGGTTTCGCCGCTGGCCCGCCGCAACGACGAAGACCCGAGCGTCACCGAC
CGCTTCGAGCTGTTCATCGGCGGCCGCGAGATCGCCAATGCCTATTCCGAGCTGAACGACGCCGAGGACCAG
GCCGAGCGCTTCATGTTGCAGGTCAAGGAGAAGGACGCCGGCGACGACGAGGCGATGCACTACGACGCCGAC
TTCATCAATGCCCTGGAATACGGCATGCCGCCGACCGCCGGCGAAGGCATCGGCATCGACCGCCTGGTGATG
CTGCTGACCAACTCGCCGTCGATCCGCGACGTGATCCTGTTCCCGCACATGCGCCCGCAGGCCTGA
Translation:
MSDQQLDQHELQQEENKLIAQRKEKLAAVREARAIAFPNDFRRDAYFADLQKQYADKTKEELEAAAIPVKVA
GRIMLNRGSFIVLQDSSERLQVYVNRKTLPEETLAEIKTWDLGDIIGAEGVLARSGKGDLYVDMTSVRLLTK
SLRPLPDKHHGLTDTEQRYRQRYVDLMVNEETRHTFRVRSQVIAHIRRFLSERGFLEVETPMLQTIPGGAAA
KPFETHHNALDMAMFLRIAPELYLKRLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMD
LTEELFRELAQAVLGTTDVPYGDKVFHFGEPFVRLSVFDSILKYNPEISAADLNDVEKARAIAKKAGAKVLG
HEGLGKLQVMIFEELVEHKLEQPHFITRYPFEVSPLARRNDEDPSVTDRFELFIGGREIANAYSELNDAEDQ
AERFMLQVKEKDAGDDEAMHYDADFINALEYGMPPTAGEGIGIDRLVMLLTNSPSIRDVILFPHMRPQA*
AnnotationID:792GeneID:783
AnnotatorUID: diggins
Modification Date/Time: 2005-04-21 12:12:09
Gene Name:lysS
Confidence Code:2
GeneProduct:lysyl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1190,KOG1885,pfam00152
Secondary Functional Category(ies):
EC Number:6.1.1.6
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67117.1|AE016340_8 (AE016779) lysyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 500

 Score =  899 bits (2323), Expect = 0.0
 Identities = 449/501 (89%), Positives = 474/501 (94%), Gaps = 1/501 (0%)

>gb|AAN81917.1|AE016766_5 (AE016766) Lysyl-tRNA synthetase [Escherichia coli CFT073]
          Length = 505

 Score =  600 bits (1548), Expect = e-171
 Identities = 308/504 (61%), Positives = 378/504 (75%), Gaps = 6/504 (1%)






 
Structural Features:
COG1190, LysU, Lysyl-tRNA synthetase (class II) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 502 residues, 100.0% aligned
Score =  709 bits (1832), Expect = 0.0
Identities = 313/503 (62%), Positives = 378/503 (75%), Gaps = 8/503 (1%)

KOG1885, Lysyl-tRNA synthetase (class II) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 560 residues,  88.8% aligned
Score =  480 bits (1236), Expect = 4e-136
Identities = 216/498 (43%), Positives = 288/498 (57%), Gaps = 20/498 (4%)

pfam00152, tRNA-synt_2, tRNA synthetases class II (D, K and N).
CD-Length = 339 residues, 100.0% aligned
Score =  400 bits (1029), Expect = 5e-112
Identities = 187/346 (54%), Positives = 230/346 (66%), Gaps = 11/346 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1569




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18711




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31850




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:783_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:783
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1395
                           PA3700_tr
                           translation of PA3700
                           501
                           99.60
                           99.99
                           0
                           2561.0
     

     
     
51009
                           gb|AAG07088.1|AE004789_8
                           (AE004789) lysyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           501
                           99.60
                           99.99
                           0
                           2561.0
     

     
     
51010
                           gb|AAN67117.1|AE016340_8
                           (AE016779) lysyl-tRNA synthetase [Pseudomonas putida KT2440]
                           501
                           89.62
                           94.61
                           1
                           2325.0
     

     
     
51011
                           gb|AAO55021.1|
                           (AE016861) lysyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           501
                           89.22
                           93.81
                           1
                           2302.0
     

     
     
51012
                           emb|CAA86924.1|
                           (Z46863) lysyl-tRNA-synthase [Acinetobacter sp. ADP1]
                           518
                           62.54
                           76.06
                           26
                           1658.0
     

     






      

          
GC ORFID: 43785How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3700Glimmer Score: 

          
Start: 3538337Stop: 3536832

          
Length: 1506


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3700 translation of PA3700Homolog Bit Score 991.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4590 (ORF: BLASTX 891.0), t_PA3700 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGACCAACAACTCGACCAGCACGAACTGCAACAGGAAGAAAACAAGCTGATCGCCCAGCGCAAGGAA 
AAACTTGCCGCCGTGCGCGAAGCCCGGGCCATCGCCTTCCCCAACGACTTCCGCCGCGACGCCTACTTCGCG 
GACCTGCAGAAACAGTACGCCGACAAGACCAAGGAAGAGCTGGAAGCCGCAGCGATCCCGGTCAAGGTGGCC 
GGTCGGATCATGCTCAACCGCGGTTCCTTCATCGTCCTCCAGGACAGCAGCGAGCGCCTGCAGGTCTACGTC 
AACCGCAAGACCCTGCCGGAAGAAACCCTGGCCGAGATCAAGACCTGGGACCTGGGCGACATCATCGGCGCC 
GAAGGCGTGCTGGCCCGCTCGGGCAAGGGCGACCTGTACGTCGACATGACCAGCGTGCGCCTGCTGACCAAG 
TCGCTGCGTCCGCTGCCGGACAAGCACCATGGCCTGACCGACACCGAGCAGCGCTACCGCCAGCGCTACGTC 
GACCTGATGGTCAACGAGGAAACCCGCCACACCTTCCGCGTGCGCTCCCAGGTCATCGCCCACATCCGCCGT 
TTCCTCAGCGAGCGCGGTTTCCTCGAAGTGGAGACCCCGATGCTGCAGACCATCCCCGGCGGTGCCGCGGCC 
AAGCCGTTCGAGACCCATCACAACGCGCTGGACATGGCCATGTTCCTGCGCATCGCCCCGGAGCTGTACCTC 
AAGCGCCTGGTGGTCGGCGGTTTCGAGAAGGTCTTCGAGATCAACCGCAACTTCCGTAACGAAGGCGTCTCG 
ACCCGGCACAACCCCGAGTTCACCATGCTCGAGTTCTACCAGGCCTACGCCGACTACGAAGACAACATGGAC 
CTGACCGAGGAACTGTTCCGCGAACTGGCCCAGGCGGTACTCGGTACCACCGACGTGCCCTACGGCGACAAG 
GTCTTCCACTTCGGCGAGCCGTTCGTGCGCCTGTCGGTGTTCGATTCGATCCTCAAGTACAACCCGGAGATC 
AGCGCCGCCGACCTCAACGACGTCGAGAAGGCGCGGGCCATCGCCAAGAAGGCCGGCGCCAAGGTCCTCGGC 
CACGAAGGCCTGGGCAAGCTGCAGGTGATGATTTTCGAGGAACTGGTGGAGCACAAGCTGGAGCAGCCGCAC 
TTCATCACCCGCTACCCCTTCGAGGTTTCGCCGCTGGCCCGCCGCAACGACGAAGACCCGAGCGTCACCGAC 
CGCTTCGAGCTGTTCATCGGCGGCCGCGAGATCGCCAATGCCTATTCCGAGCTGAACGACGCCGAGGACCAG 
GCCGAGCGCTTCATGTTGCAGGTCAAGGAGAAGGACGCCGGCGACGACGAGGCGATGCACTACGACGCCGAC 
TTCATCAATGCCCTGGAATACGGCATGCCGCCGACCGCCGGCGAAGGCATCGGCATCGACCGCCTGGTGATG 
CTGCTGACCAACTCGCCGTCGATCCGCGACGTGATCCTGTTCCCGCACATGCGCCCGCAGGCCTGA