ViewGene
Locus Name:
GeneID:
Location:

GeneID:782
TrivialName: PA14_70690
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70690
PAO1 Ortholog Locus:PA5355
Sequence Length:1500
Protein Length:499
Start: 6296621
Stop: 6295122
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08740.1|AE004947_10 (AE004947) glycolate oxidase subunit GlcD [Pseudomonas aeruginosa PAO1]
Identities = 498/499 (99%), Positives = 498/499 (99%)
Sequence:
ATGAACATCCTCTACGACGAGCGTCTGGACGGTCCGCTGCCGCAGGTGGACAAGGATGGCCTGCTGGCCGAG
TTGCGCCTCCGCCTGCCCGACCTCGAACTGCTGCACGCCGCGGAAGACCTGCGTCCCTACGAGTGCGACGGA
CTCTCGGCGTATCGCTGCACGCCGTTGCTGGTGGCATTGCCGGAACGCATCGAACAGGTTCAGGGCCTGCTC
GCCCTCTGCCATCGGCTGAAGGTGCCGGTCGTGGCGCGCGGTGCCGGCACCGGCCTGTCCGGCGGCGCGCTG
CCGCTGGAGAACGGCGTGCTGCTGGTGATGGCGCGTTTCCGGCGGATCCTCGAGATCGATCCGCTGGGTCGT
TTCGCCCGGGTCCAGCCTGGCGTGCGCAACCTGGCGATCTCCCAGGCCGCTGCCCCTCATGGTCTGTACTAC
GCACCGGACCCGTCCTCGCAGATCGCCTGCTCCATCGGCGGCAATGTCGCGGAGAACGCCGGCGGCGTGCAT
TGCCTGAAGTACGGCCTGACCGTGCACAACCTGTTGCAGGTGGACATCGTGACCCTGGAAGGCGAGCGCCTG
AGCCTGGGGTCCAGCGCCCTGGACAGCGCCGGCTTCGACCTGCTGGCGCTGTTCACCGGCTCCGAAGGGCTG
CTCGGCGTGGTGGTGGAGGTCACCGTCAGGCTGTTGCCGCGCCCGCCGGTGGCGAAGGTCCTGCTGGCCAGC
TTCGACGATGTGGAAAGCGCCGGGCGGGCGGTGGCCGACCTGATCGGTGCCGGCATCGTCCCGGCCGGGCTG
GAAATGATGGACAACCTGTCGATCCGCGCCGCCGAGGACTTCATCCATGCCGGCTATCCGGTCGACGCGGCG
GCGATCCTGCTCTGCGAACTGGATGGCGTGGAAGCCGACGTGCACGAGGATTGCGAGCGGGTCCGCGAGCTG
TTCGAGGCGGCTGGCGCCACATCGGTGCGACAGGCCCAAGACGAGGCCGAGCGCCAGCGCTTCTGGGCCGGG
CGCAAGAATGCGTTTCCGGCGGTCGGGCGGATTTCCCCCGATTACTACTGCATGGACGGCAGCATCCCGCGC
CGCGAGCTGCCGCGGGTGCTGCATGGCATCGCCGAGCTTTCGCGCGAATACGGCCTGCGGGTGGCCAACGTA
TTCCACGCCGGCGACGGCAACATGCACCCGCTGATCCTCTTCGACGCCAACCTGCCGGGCGAGCTGGAGCGC
GCCGAAGCGCTGGGCGGCAGGATCCTCGAGTTGTGCGTGGCGGTAGGCGGCAGCATCACCGGCGAACATGGC
GTCGGCCGCGAGAAGATCAACCAGATGTGCGCTCAGTTCAATGCCGACGAGCTGACCCTGTTCCACGCCGTG
AAGGCGGCGTTCGACCCGGCCGGGCTGCTCAATCCCGGCAAGAACGTGCCCACCCTGCATCGCTGCGCCGAG
TTCGGCGCCATGCACGTCCACCACGGTCGACTGCCGTTCCCCGAACTGGAGCGTTTCTGA
Translation:
MNILYDERLDGPLPQVDKDGLLAELRLRLPDLELLHAAEDLRPYECDGLSAYRCTPLLVALPERIEQVQGLL
ALCHRLKVPVVARGAGTGLSGGALPLENGVLLVMARFRRILEIDPLGRFARVQPGVRNLAISQAAAPHGLYY
APDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLQVDIVTLEGERLSLGSSALDSAGFDLLALFTGSEGL
LGVVVEVTVRLLPRPPVAKVLLASFDDVESAGRAVADLIGAGIVPAGLEMMDNLSIRAAEDFIHAGYPVDAA
AILLCELDGVEADVHEDCERVRELFEAAGATSVRQAQDEAERQRFWAGRKNAFPAVGRISPDYYCMDGSIPR
RELPRVLHGIAELSREYGLRVANVFHAGDGNMHPLILFDANLPGELERAEALGGRILELCVAVGGSITGEHG
VGREKINQMCAQFNADELTLFHAVKAAFDPAGLLNPGKNVPTLHRCAEFGAMHVHHGRLPFPELERF*
AnnotationID:791GeneID:782
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 07:07:17
Gene Name:glcD
Confidence Code:2
GeneProduct:glycolate oxidase subunit GlcD
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0277,pfam02913,pfam01565
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69340.1|AE016571_6 (AE016788) glycolate oxidase, subunit GlcD [Pseudomonas putida
           KT2440]
          Length = 499

 Score =  850 bits (2195), Expect = 0.0
 Identities = 417/499 (83%), Positives = 453/499 (90%)

>gb|AAO57030.1| (AE016868) glycolate oxidase, subunit GlcD [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 499

 Score =  838 bits (2165), Expect = 0.0
 Identities = 410/499 (82%), Positives = 449/499 (89%)

>gb|AAN82153.1|AE016766_241 (AE016766) Glycolate oxidase subunit glcD [Escherichia coli CFT073]
          Length = 499

 Score =  818 bits (2114), Expect = 0.0
 Identities = 399/499 (79%), Positives = 441/499 (88%)






 
Structural Features:
COG0277, GlcD, FAD/FMN-containing dehydrogenases [Energy production and conversion]
CD-Length = 459 residues, 100.0% aligned
Score =  335 bits (859), Expect = 7e-93
Identities = 199/462 (43%), Positives = 253/462 (54%), Gaps = 13/462 (2%)

pfam02913, FAD-oxidase_C, FAD linked oxidases, C-terminal domain.
CD-Length = 247 residues,  99.6% aligned
Score =  212 bits (542), Expect = 5e-56
Identities = 111/246 (45%), Positives = 144/246 (58%), Gaps = 5/246 (2%)

pfam01565, FAD_binding_4, FAD binding domain.
CD-Length = 158 residues,  99.4% aligned
Score =  141 bits (358), Expect = 1e-34
Identities = 64/160 (40%), Positives = 89/160 (55%), Gaps = 3/160 (1%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1567




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18709




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34760




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:782_cdd.html


    		




 
URL:

















    ReferenceID:35682




  
Author/Investigator(s):
        Pellicer MT, Badia J, Aguilar J, Baldoma L.





  
Title:
        glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein.




  
PubMed:
        8606183


MedLine:
        




    
Source:
J Bacteriol. 1996 Apr;178(7):2051-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8606183









           
Homologs By Global Alignment 

           

       



     
Gene ID:782
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1394
                           PA5355_tr
                           translation of PA5355
                           499
                           99.79
                           99.79
                           0
                           2564.0
     

     
     
50973
                           gb|AAG08740.1|AE004947_10
                           (AE004947) glycolate oxidase subunit GlcD [Pseudomonas aeruginosa PAO1]
                           499
                           99.79
                           99.79
                           0
                           2564.0
     

     
     
50974
                           gb|AAN69340.1|AE016571_6
                           (AE016788) glycolate oxidase, subunit GlcD [Pseudomonas putida KT2440]
                           499
                           83.56
                           90.78
                           0
                           2195.0
     

     
     
50975
                           gb|AAO57030.1|
                           (AE016868) glycolate oxidase, subunit GlcD [Pseudomonas syringae pv. tomato str. DC3000]
                           499
                           82.16
                           89.97
                           0
                           2165.0
     

     
     
50976
                           gb|AAN82153.1|AE016766_241
                           (AE016766) Glycolate oxidase subunit glcD [Escherichia coli CFT073]
                           499
                           79.95
                           88.37
                           0
                           2114.0
     

     






      

          
GC ORFID: 43784How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5355Glimmer Score: 

          
Start: 5560310Stop: 5558811

          
Length: 1500


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5355 translation of PA5355Homolog Bit Score 992.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8165 (ORF: BLASTX 843.0), t_PA5355 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACATCCTCTACGACGAGCGTCTGGACGGTCCGCTGCCGCAGGTGGACAAGGATGGCCTGCTGGCCGAG 
TTGCGCCTCCGCCTGCCCGACCTCGAACTGCTGCACGCCGCGGAAGACCTGCGTCCCTACGAGTGCGACGGA 
CTCTCGGCGTATCGCTGCACGCCGTTGCTGGTGGCATTGCCGGAACGCATCGAACAGGTTCAGGGCCTGCTC 
GCCCTCTGCCATCGGCTGAAGGTGCCGGTCGTGGCGCGCGGTGCCGGCACCGGCCTGTCCGGCGGCGCGCTG 
CCGCTGGAGAACGGCGTGCTGCTGGTGATGGCGCGTTTCCGGCGGATCCTCGAGATCGATCCGCTGGGTCGT 
TTCGCCCGGGTCCAGCCTGGCGTGCGCAACCTGGCGATCTCCCAGGCCGCTGCCCCTCATGGTCTGTACTAC 
GCACCGGACCCGTCCTCGCAGATCGCCTGCTCCATCGGCGGCAATGTCGCGGAGAACGCCGGCGGCGTGCAT 
TGCCTGAAGTACGGCCTGACCGTGCACAACCTGTTGCAGGTGGACATCGTGACCCTGGAAGGCGAGCGCCTG 
AGCCTGGGGTCCAGCGCCCTGGACAGCGCCGGCTTCGACCTGCTGGCGCTGTTCACCGGCTCCGAAGGGCTG 
CTCGGCGTGGTGGTGGAGGTCACCGTCAGGCTGTTGCCGCGCCCGCCGGTGGCGAAGGTCCTGCTGGCCAGC 
TTCGACGATGTGGAAAGCGCCGGGCGGGCGGTGGCCGACCTGATCGGTGCCGGCATCGTCCCGGCCGGGCTG 
GAAATGATGGACAACCTGTCGATCCGCGCCGCCGAGGACTTCATCCATGCCGGCTATCCGGTCGACGCGGCG 
GCGATCCTGCTCTGCGAACTGGATGGCGTGGAAGCCGACGTGCACGAGGATTGCGAGCGGGTCCGCGAGCTG 
TTCGAGGCGGCTGGCGCCACATCGGTGCGACAGGCCCAAGACGAGGCCGAGCGCCAGCGCTTCTGGGCCGGG 
CGCAAGAATGCGTTTCCGGCGGTCGGGCGGATTTCCCCCGATTACTACTGCATGGACGGCAGCATCCCGCGC 
CGCGAGCTGCCGCGGGTGCTGCATGGCATCGCCGAGCTTTCGCGCGAATACGGCCTGCGGGTGGCCAACGTA 
TTCCACGCCGGCGACGGCAACATGCACCCGCTGATCCTCTTCGACGCCAACCTGCCGGGCGAGCTGGAGCGC 
GCCGAAGCGCTGGGCGGCAGGATCCTCGAGTTGTGCGTGGCGGTAGGCGGCAGCATCACCGGCGAACATGGC 
GTCGGCCGCGAGAAGATCAACCAGATGTGCGCTCAGTTCAATGCCGACGAGCTGACCCTGTTCCACGCCGTG 
AAGGCGGCGTTCGACCCGGCCGGGCTGCTCAATCCCGGCAAGAACGTGCCCACCCTGCATCGCTGCGCCGAG 
TTCGGCGCCATGCACGTCCACCACGGTCGACTGCCGTTCCCCGAACTGGAGCGTTTCTGA