ViewGene
Locus Name:
GeneID:
Location:

GeneID:78
TrivialName: PA14_62530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62530
PAO1 Ortholog Locus:PA4725
Sequence Length:2952
Protein Length:983
Start: 5575942
Stop: 5578893
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08111.1|AE004886_3 (AE004886) two-component sensor CbrA [Pseudomonas aeruginosa PAO1]
Identities = 982/983 (99%), Positives = 982/983 (99%)
Sequence:
ATGCTGACGAGCTTTAGCCTGACCCAGCTGATCCTGATCAGCGTCGCCTACCTCTCCACCCTCTTCGGCATC
GCCTGGATCACCGAGCGCGGCTACGTGCCGCGCCGGCTGGTACGCCACCCGCTGGTCTACACCCTGTCGTTG
GGCGTCTACGCCAGTGCCTGGGCGTTCTACGGCACGGTAGGCCTGGCCTACCAGTACGGCTACGGGTTCCTC
GCCATCTACCTGGGGATTTCCGGGGCCTTCCTGCTCGCCCCGGTGCTGCTCTATCCGATCCTGCGGGTGACA
CGCGCCTACCAGCTGTCCTCGCTGGCCGACCTCTTCGCCTTCCGTTTCCGCAGTACCTGGGCCGGCGCCCTG
ACCACGCTGTTCATGCTGATCGGCGTGCTGCCGATGCTGGCCCTGCAGATCCAGGCGGTCACCGACTCGATC
AGCATCCTCACCCGCGACCCGGCCCAGGAGCGGGTCGCGTTCGTCTTCTGCACCCTGATCACCCTGTTCGCG
ATCCTCTTCGGCGCTCGCCACATCGCCACCCGCGAACGCCACGAGGGACTGGTGTTCGCCATCGCCTTCGAG
TCGCTGGTCAAGCTGGTGATGCTCGGCAGTATCGGCCTCTACGCCCTGTACGGCGTCTTCGGCGGGCCGGAA
GGCCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCGCTGACCACCCTGCACACGCCGCTGGCCGAGGGTCCA
TGGCGAACGCTGCTGCTGGTGTTCTTCGCCGCGGCCATCGTGATGCCGCACATGTTCCACATGATCTTCACC
GAGAACCTCAACCCGCGCGCGCTGATTTCCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTT
GCGGTACCGCCGATCCTCTGGGCCGGGCTGCGCCTCGGCGCCTCGACCACACCGGAGTACTACACCATCGGC
CTCGGCCTGGCGGTGGACAGCCCGGCCCTGGCCCTGGCCGCCTTCATCGGCGGCATCTCCGCCGCCAGCGGG
CTGACTATCGTGATGACCCTGGCGCTCTCGGGAATGGTCCTCAACCACCTGGTGCTGCCGCTCTACCAGCCG
CCGGCGCAGGGCAACATCTACCGCTGGCTGAAATGGACCCGGCGCCTGCTGATCGTCGCCATCATCATGGCC
AGCTACGCCTTCTACCTGCTGCTGGGCGCCGAACAGGACCTCTCCAACCTGGGCATAGTGTCCTTCGTCGCC
ACCCTGCAATTCCTTCCCGGCGCCCTCTCGGTGCTGTACTGGCCGACCGCCAACCGCCGCGGCTTCATCTCC
GGGCTGATCGCCGGCATGCTGGTCTGGGCGATCACCATGCTGCTGCCCCTGATGGGGAACGTACAGGGCCTG
TACCTGCCGCTGTTCGACGTGATCTACGTGCTGGACGACTCCAACTGGCACCTGGCGGCGCTGTCCTCGCTG
GCGGCCAACGTGCTGGTATTCACCCTGGTCTCGCTGTTCACCGAGGCCAGCGACGAGGAAAAAGGCGCCGCC
GAGGCCTGCGCGGTGGACAACGTACGCCGTCCGCAACGCCGCGAACTGTTCGCCGGCTCGCCGCAGGAGTTC
GCCAGCCAGCTGGCCAAGCCGCTGGGCGCCAAGACCGCGCAGAAGGAGGTCGAGCAGGCCCTGCGCGATCTC
CACCTGCCCTTCGACGAACGTCGCCCCTATGCCCTGCGTCGCTTGCGCGACCGTATCGAGGCGAACCTTTCC
GGGCTGATGGGTCCCAGTGTCGCCCAGGACATCGTGGAGACCTTCCTTCCCTACAAGACCAGCGAAGAAAGC
TATGTCACCGAGGACATCCACTTCATCGAGAGCCGCCTGGAGGACTATCACTCCCGGCTGACCGGCCTCGCC
GCCGAACTCGACACCCTGCGCCGCTACCACCGGCAGACCCTGCAAGACCTGCCGATGGGCGTCTGCTCCCTG
GCCAAGGACCAGGAAGTGCTGATGTGGAACCGCGCCATCGAGGAACTCACCGGGGTCGGCGCGCAGAAGGTC
GTCGGTTCGCGCCTGTCGGCCTTGCCCGAACCCTGGAAGGGCCTGCTGGAACGTTTCATCGACGCCCCCGAC
GAGCACCTGCACAAGCAGCGCCTGGTCTACGACGGCCACACCCGCTGGCTGAACCTGCACAAGGCGGCCATC
GAGGAACCCCTGGCGCCGGGTAACAGCGGCCTGGTGCTACTGGTGGAAGACCTCACCGAAACCCAACTGCTG
GAAGACAAGCTGGTCCACTCCGAACGCCTGGCTTCCATCGGCCGCCTTGCCGCCGGGGTAGCCCACGAGATC
GGCAATCCGATCACCGGCATCGCCTGCCTGGCGCAGAACCTGCGCGAGGAGCGCGAAGGCGACGGCGAGCTG
ACCGAGATTAGCGAGCAGATCCTCGACCAGACCAAGCGCGTATCGCGCATCGTCCAGTCGTTGATGAGCTTC
GCCCACTCCGGTAGCCACCTGCAGGCCCTGGAACCGGTGTGCCTGAGCGAAGTCGCCCAGGAGGCCATCGGA
CTGCTGTCGCTGAACCGGCGTAGCGTCGAAGTAGAGTTCTTCAACCTCTGCGATCCGGCGCACTGGGTCGAG
GGCGACTCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAATGCTCGCGACGCCAGCCCGCCGGGC
GGCGCGATCCGGGTCCGCAGCGAAGCCTCGGAACATACCGTGGACCTGGTCGTCGAGGACGAGGGCAGCGGC
ATCCCGAAGGCGATCATGGACCAGTTGTTCGAACCGTTCTTCACCACCAAGGACCCCGGTAAGGGGACCGGG
CTCGGCCTCGCACTGGTCTATTCGATCGTGGAAGAGCATTATGGACAGATAACCATCGAGAGCCCGACGGAT
CACCAGCGAGAAGGCGGCACCCGCTTCCGCGTGACCCTGCCGCGGCACCCTGGCCCGACGGCCGAGCTGTAG
Translation:
MLTSFSLTQLILISVAYLSTLFGIAWITERGYVPRRLVRHPLVYTLSLGVYASAWAFYGTVGLAYQYGYGFL
AIYLGISGAFLLAPVLLYPILRVTRAYQLSSLADLFAFRFRSTWAGALTTLFMLIGVLPMLALQIQAVTDSI
SILTRDPAQERVAFVFCTLITLFAILFGARHIATRERHEGLVFAIAFESLVKLVMLGSIGLYALYGVFGGPE
GLEVWLLQNQTALTTLHTPLAEGPWRTLLLVFFAAAIVMPHMFHMIFTENLNPRALISASWGLPLFLLLMSL
AVPPILWAGLRLGASTTPEYYTIGLGLAVDSPALALAAFIGGISAASGLTIVMTLALSGMVLNHLVLPLYQP
PAQGNIYRWLKWTRRLLIVAIIMASYAFYLLLGAEQDLSNLGIVSFVATLQFLPGALSVLYWPTANRRGFIS
GLIAGMLVWAITMLLPLMGNVQGLYLPLFDVIYVLDDSNWHLAALSSLAANVLVFTLVSLFTEASDEEKGAA
EACAVDNVRRPQRRELFAGSPQEFASQLAKPLGAKTAQKEVEQALRDLHLPFDERRPYALRRLRDRIEANLS
GLMGPSVAQDIVETFLPYKTSEESYVTEDIHFIESRLEDYHSRLTGLAAELDTLRRYHRQTLQDLPMGVCSL
AKDQEVLMWNRAIEELTGVGAQKVVGSRLSALPEPWKGLLERFIDAPDEHLHKQRLVYDGHTRWLNLHKAAI
EEPLAPGNSGLVLLVEDLTETQLLEDKLVHSERLASIGRLAAGVAHEIGNPITGIACLAQNLREEREGDGEL
TEISEQILDQTKRVSRIVQSLMSFAHSGSHLQALEPVCLSEVAQEAIGLLSLNRRSVEVEFFNLCDPAHWVE
GDSQRLAQVLINLLSNARDASPPGGAIRVRSEASEHTVDLVVEDEGSGIPKAIMDQLFEPFFTTKDPGKGTG
LGLALVYSIVEEHYGQITIESPTDHQREGGTRFRVTLPRHPGPTAEL*
AnnotationID:88GeneID:78
AnnotatorUID: diggins
Modification Date/Time: 2005-03-09 07:07:54
Gene Name:cbrA
Confidence Code:1
GeneProduct:two-component sensor CbrA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:signal sensor and protein histidine kinase
Functional Category Confidence Code:5
COGs:COG0591,smart00387,pfam02518,cd00075
Secondary Functional Category(ies):signal transduction mechanisms
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70268.1|AE016667_1 (AE016791) sensory box histidine kinase [Pseudomonas putida KT2440]
          Length = 991

 Score = 1625 bits (4209), Expect = 0.0
 Identities = 803/977 (82%), Positives = 885/977 (90%), Gaps = 1/977 (0%)

>gb|AAO54499.1| (AE016859) sensor histidine kinase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 982

 Score = 1615 bits (4182), Expect = 0.0
 Identities = 792/978 (80%), Positives = 891/978 (91%)








 
Structural Features:
COG0591, PutP, Na+/proline symporter [Amino acid transport and metabolism]
CD-Length = 493 residues,  99.8% aligned
Score =  230 bits (588), Expect = 6e-61
Identities = 118/513 (23%), Positives = 196/513 (38%), Gaps = 30/513 (5%)

smart00387, HATPase_c, Histidine kinase-like ATPases
CD-Length = 112 residues,  97.3% aligned
Score =  113 bits (283), Expect = 1e-25
Identities = 53/113 (46%), Positives = 68/113 (60%), Gaps = 7/113 (6%)

pfam02518, HATPase_c, Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase.
CD-Length = 111 residues,  98.2% aligned
Score =  106 bits (266), Expect = 1e-23
Identities = 56/113 (49%), Positives = 68/113 (60%), Gaps = 7/113 (6%)

cd00075, HATPase_c, Histidine kinase-like ATPases
CD-Length = 103 residues, 100.0% aligned
Score = 97.7 bits (243), Expect = 6e-21
Identities = 46/107 (42%), Positives = 65/107 (60%), Gaps = 7/107 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:159




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17301




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37654




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:78_cdd.html


    		




 
URL:

















    ReferenceID:37742




  
Author/Investigator(s):
        Nishijyo T, Haas D, Itoh Y.





  
Title:
        The CbrA-CbrB two-component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosa.




  
PubMed:
        11401699


MedLine:
        




    
Source:
Mol Microbiol. 2001 May;40(4):917-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11401699









           
Homologs By Global Alignment 

           

       



     
Gene ID:78
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
155
                           PA4725_tr
                           translation of PA4725
                           983
                           99.89
                           99.89
                           0
                           4992.0
     

     
     
13629
                           gb|AAG08111.1|AE004886_3
                           (AE004886) two-component sensor CbrA [Pseudomonas aeruginosa PAO1]
                           983
                           99.89
                           99.89
                           0
                           4992.0
     

     
     
13630
                           dbj|BAB20866.1|
                           (AB045630) CbrA [Pseudomonas aeruginosa]
                           983
                           99.89
                           99.89
                           0
                           4992.0
     

     
     
13631
                           gb|AAN70268.1|AE016667_1
                           (AE016791) sensory box histidine kinase [Pseudomonas putida KT2440]
                           991
                           81.23
                           89.70
                           8
                           4208.0
     

     
     
13632
                           gb|AAO54499.1|
                           (AE016859) sensor histidine kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           984
                           80.48
                           90.54
                           3
                           4179.0
     

     






      

          
GC ORFID: 43080How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4725Glimmer Score: 

          
Start: 4839622Stop: 4842573

          
Length: 2952


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4725 translation of PA4725Homolog Bit Score 1927.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2515 (ORF: BLASTX 1613.0), t_PA4725 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGACGAGCTTTAGCCTGACCCAGCTGATCCTGATCAGCGTCGCCTACCTCTCCACCCTCTTCGGCATC 
GCCTGGATCACCGAGCGCGGCTACGTGCCGCGCCGGCTGGTACGCCACCCGCTGGTCTACACCCTGTCGTTG 
GGCGTCTACGCCAGTGCCTGGGCGTTCTACGGCACGGTAGGCCTGGCCTACCAGTACGGCTACGGGTTCCTC 
GCCATCTACCTGGGGATTTCCGGGGCCTTCCTGCTCGCCCCGGTGCTGCTCTATCCGATCCTGCGGGTGACA 
CGCGCCTACCAGCTGTCCTCGCTGGCCGACCTCTTCGCCTTCCGTTTCCGCAGTACCTGGGCCGGCGCCCTG 
ACCACGCTGTTCATGCTGATCGGCGTGCTGCCGATGCTGGCCCTGCAGATCCAGGCGGTCACCGACTCGATC 
AGCATCCTCACCCGCGACCCGGCCCAGGAGCGGGTCGCGTTCGTCTTCTGCACCCTGATCACCCTGTTCGCG 
ATCCTCTTCGGCGCTCGCCACATCGCCACCCGCGAACGCCACGAGGGACTGGTGTTCGCCATCGCCTTCGAG 
TCGCTGGTCAAGCTGGTGATGCTCGGCAGTATCGGCCTCTACGCCCTGTACGGCGTCTTCGGCGGGCCGGAA 
GGCCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCGCTGACCACCCTGCACACGCCGCTGGCCGAGGGTCCA 
TGGCGAACGCTGCTGCTGGTGTTCTTCGCCGCGGCCATCGTGATGCCGCACATGTTCCACATGATCTTCACC 
GAGAACCTCAACCCGCGCGCGCTGATTTCCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTT 
GCGGTACCGCCGATCCTCTGGGCCGGGCTGCGCCTCGGCGCCTCGACCACACCGGAGTACTACACCATCGGC 
CTCGGCCTGGCGGTGGACAGCCCGGCCCTGGCCCTGGCCGCCTTCATCGGCGGCATCTCCGCCGCCAGCGGG 
CTGACTATCGTGATGACCCTGGCGCTCTCGGGAATGGTCCTCAACCACCTGGTGCTGCCGCTCTACCAGCCG 
CCGGCGCAGGGCAACATCTACCGCTGGCTGAAATGGACCCGGCGCCTGCTGATCGTCGCCATCATCATGGCC 
AGCTACGCCTTCTACCTGCTGCTGGGCGCCGAACAGGACCTCTCCAACCTGGGCATAGTGTCCTTCGTCGCC 
ACCCTGCAATTCCTTCCCGGCGCCCTCTCGGTGCTGTACTGGCCGACCGCCAACCGCCGCGGCTTCATCTCC 
GGGCTGATCGCCGGCATGCTGGTCTGGGCGATCACCATGCTGCTGCCCCTGATGGGGAACGTACAGGGCCTG 
TACCTGCCGCTGTTCGACGTGATCTACGTGCTGGACGACTCCAACTGGCACCTGGCGGCGCTGTCCTCGCTG 
GCGGCCAACGTGCTGGTATTCACCCTGGTCTCGCTGTTCACCGAGGCCAGCGACGAGGAAAAAGGCGCCGCC 
GAGGCCTGCGCGGTGGACAACGTACGCCGTCCGCAACGCCGCGAACTGTTCGCCGGCTCGCCGCAGGAGTTC 
GCCAGCCAGCTGGCCAAGCCGCTGGGCGCCAAGACCGCGCAGAAGGAGGTCGAGCAGGCCCTGCGCGATCTC 
CACCTGCCCTTCGACGAACGTCGCCCCTATGCCCTGCGTCGCTTGCGCGACCGTATCGAGGCGAACCTTTCC 
GGGCTGATGGGTCCCAGTGTCGCCCAGGACATCGTGGAGACCTTCCTTCCCTACAAGACCAGCGAAGAAAGC 
TATGTCACCGAGGACATCCACTTCATCGAGAGCCGCCTGGAGGACTATCACTCCCGGCTGACCGGCCTCGCC 
GCCGAACTCGACACCCTGCGCCGCTACCACCGGCAGACCCTGCAAGACCTGCCGATGGGCGTCTGCTCCCTG 
GCCAAGGACCAGGAAGTGCTGATGTGGAACCGCGCCATCGAGGAACTCACCGGGGTCGGCGCGCAGAAGGTC 
GTCGGTTCGCGCCTGTCGGCCTTGCCCGAACCCTGGAAGGGCCTGCTGGAACGTTTCATCGACGCCCCCGAC 
GAGCACCTGCACAAGCAGCGCCTGGTCTACGACGGCCACACCCGCTGGCTGAACCTGCACAAGGCGGCCATC 
GAGGAACCCCTGGCGCCGGGTAACAGCGGCCTGGTGCTACTGGTGGAAGACCTCACCGAAACCCAACTGCTG 
GAAGACAAGCTGGTCCACTCCGAACGCCTGGCTTCCATCGGCCGCCTTGCCGCCGGGGTAGCCCACGAGATC 
GGCAATCCGATCACCGGCATCGCCTGCCTGGCGCAGAACCTGCGCGAGGAGCGCGAAGGCGACGGCGAGCTG 
ACCGAGATTAGCGAGCAGATCCTCGACCAGACCAAGCGCGTATCGCGCATCGTCCAGTCGTTGATGAGCTTC 
GCCCACTCCGGTAGCCACCTGCAGGCCCTGGAACCGGTGTGCCTGAGCGAAGTCGCCCAGGAGGCCATCGGA 
CTGCTGTCGCTGAACCGGCGTAGCGTCGAAGTAGAGTTCTTCAACCTCTGCGATCCGGCGCACTGGGTCGAG 
GGCGACTCGCAGCGCCTGGCCCAGGTGCTGATCAACCTGCTCTCCAATGCTCGCGACGCCAGCCCGCCGGGC 
GGCGCGATCCGGGTCCGCAGCGAAGCCTCGGAACATACCGTGGACCTGGTCGTCGAGGACGAGGGCAGCGGC 
ATCCCGAAGGCGATCATGGACCAGTTGTTCGAACCGTTCTTCACCACCAAGGACCCCGGTAAGGGGACCGGG 
CTCGGCCTCGCACTGGTCTATTCGATCGTGGAAGAGCATTATGGACAGATAACCATCGAGAGCCCGACGGAT 
CACCAGCGAGAAGGCGGCACCCGCTTCCGCGTGACCCTGCCGCGGCACCCTGGCCCGACGGCCGAGCTGTAG