ViewGene
Locus Name:
GeneID:
Location:

GeneID:775
TrivialName: PA14_18010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18010
PAO1 Ortholog Locus:PA3579
Sequence Length:1485
Protein Length:494
Start: 1546003
Stop: 1547487
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06967.1|AE004778_11 (AE004778) probable carbohydrate kinase [Pseudomonas aeruginosa PAO1]
Identities = 491/494 (99%), Positives = 492/494 (99%)
Sequence:
ATGTCCCACTATCTCCTCGCCATCGACCAGGGCACCACCAGCTCCCGCGCCATCGTGTTCAGTGCCCAGGGC
TTGCCGGTGACGAGCTGCCAGCAGGAATTCAAGCAGTACTTCCCGAAGGACGGCTGGGTGGAGCACGACGGC
GAGGAAATCTGGCTGACCACCCTCCAGGTCTGTCGCGACGCGCTCGCTCGCAAGGGCTTGCGCGCGGGCGAC
ATCGCCGCCATCGGCATCACCAACCAGCGCGAGACCACGCTGGTCTGGGACGCCGCCAGCGGCGACCTGATC
CACCCGGCCATCGTCTGGCAGGATCGTCGTACCGCCGACTACTGTGCCGAACTCAAGGCCGCCGGCCACGAG
GCGAACGTCAGCGCGCGCACCGGGCTGCTGATCGATCCGTATTTCTCCGCCACCAAGCTGCGCTGGATCCTC
GACAACGTGCCCGGCGCCCGCCAGCGCGCCGAACGCGGCGAGCTGCGCTTCGGCACCGTCGACTGCTTCCTG
CTCTGGCGCCTGACCGGCGGCCGCTCCCATCGCACCGACGCGACCAACGCTTCGCGCACCCTGCTGTTCAAC
ATCCACAGCCAGGACTGGGACGAGGAATTGCTGGCGCTGTTCGAGATCCCGCGCAGCCTGCTGCCGGAGGTA
CTCGACTGCGCGGCGGAGTTCGGCGTCAGCGAGCCGTCGCTGCTTGGCGCGGCGATCCCGGTGCTGGGCATG
GCCGGCGACCAGCAGGCCGCGCTGATCGGCCAGGCCTGCTTCCAGCCGGGCATGGTGAAGAGCACCTACGGC
ACCGGCTGCTTCATGATCCAGAACACTGGCGAGCAGCCGGTGACCTCGAAGAACCGCCTGCTCACCACGGTT
GGCTACCGTCTGGACGGCAAGGTCAGCTACGCGGTGGAGGGCAGCATCTTCGTCGCCGGCGCCGCGGTGCAA
TGGCTGCGCGACGGCATCAAGCTGATCGACCACGCCCACGAGAGCGAAGCCCTGGCGATCCAGGCCGGCGAC
TCCAACGGCGTCTACCTGGTGCCGGCCTTTACCGGCCTCGGCGCGCCCTACTGGGACCCGAAGGCGCGCGGC
GCGATCTTCGGCCTGACCCGCGACACCGGAATCAAGGAGATCGTCACCGCCGGTCTGCAATCGGTCTGCTAC
CAGACCCGCGACCTGCTCGAAGCGATGCGCCAGGACGGCACGCCGCCCAGCGCGCTGCGGGTGGACGGCGGG
ATGGTGGTGAACAACTGGGTGATGCAATTTCTCACCGACATCCTTGGTGTCACCGTGGAGCGCCCGGAAGTC
ACCGAGACCACCGCCCTCGGCGTGGCCTACATGGCCGGGCTGAAGGCAGGCTTCTATCGCGACCTGGACGAC
ATCGCCAGCCACTGGCACCTGCAGCGCCGCTTCGCCGCACACATGGCCGAGGAGCGCCGCGGCGAACTCTAC
GCCGGCTGGCAGAACGCGGTGCGACGGGTGCGCAGCGAGGCCTGA
Translation:
MSHYLLAIDQGTTSSRAIVFSAQGLPVTSCQQEFKQYFPKDGWVEHDGEEIWLTTLQVCRDALARKGLRAGD
IAAIGITNQRETTLVWDAASGDLIHPAIVWQDRRTADYCAELKAAGHEANVSARTGLLIDPYFSATKLRWIL
DNVPGARQRAERGELRFGTVDCFLLWRLTGGRSHRTDATNASRTLLFNIHSQDWDEELLALFEIPRSLLPEV
LDCAAEFGVSEPSLLGAAIPVLGMAGDQQAALIGQACFQPGMVKSTYGTGCFMIQNTGEQPVTSKNRLLTTV
GYRLDGKVSYAVEGSIFVAGAAVQWLRDGIKLIDHAHESEALAIQAGDSNGVYLVPAFTGLGAPYWDPKARG
AIFGLTRDTGIKEIVTAGLQSVCYQTRDLLEAMRQDGTPPSALRVDGGMVVNNWVMQFLTDILGVTVERPEV
TETTALGVAYMAGLKAGFYRDLDDIASHWHLQRRFAAHMAEERRGELYAGWQNAVRRVRSEA*
AnnotationID:784GeneID:775
AnnotatorUID: diggins
Modification Date/Time: 2005-07-05 05:05:17
Gene Name:
Confidence Code:3
GeneProduct:putative glycerol kinase
Cell Localization:()
Synonyms:glpK
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0554,KOG2517,COG1070
Secondary Functional Category(ies):energy metabolism
EC Number:2.7.1.30
Status:ACTIVE
Pathway:
Glycerolipid metabolism.
Glycerol utilization; rate-limiting step.
Homology:
>emb|CAC49535.1| (AL603646) probable glycerol kinase, similar to sugar kinases
           protein [Sinorhizobium meliloti]
          Length = 497

 Score =  607 bits (1566), Expect = e-173
 Identities = 300/492 (60%), Positives = 367/492 (74%), Gaps = 3/492 (0%)

>dbj|BAA97790.1| (AB039932) glpK gene homolog [Rhizobium rhizogenes] [Agrobacterium
           rhizogenes]
          Length = 500

 Score =  571 bits (1471), Expect = e-162
 Identities = 287/497 (57%), Positives = 356/497 (71%), Gaps = 3/497 (0%)
Structural Features:
COG0554, GlpK, Glycerol kinase [Energy production and conversion]
CD-Length = 499 residues,  99.2% aligned
Score =  778 bits (2012), Expect = 0.0
Identities = 310/495 (62%), Positives = 377/495 (76%), Gaps = 1/495 (0%)

KOG2517, Ribulose kinase and related carbohydrate kinases 
[Carbohydrate transport and metabolism]
CD-Length = 516 residues,  96.3% aligned
Score =  380 bits (976), Expect = 6e-106
Identities = 199/499 (39%), Positives = 277/499 (55%), Gaps = 17/499 (3%)

COG1070, XylB, Sugar (pentulose and hexulose) kinases 
[Carbohydrate transport and metabolism]
CD-Length = 502 residues,  97.8% aligned
Score =  262 bits (671), Expect = 1e-70
Identities = 158/503 (31%), Positives = 229/503 (45%), Gaps = 23/503 (4%)
Genomic Context:






   
Comment:


















    ReferenceID:1553




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18695




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31963




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type: (text/plain)







    
Data:775_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:775
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1379
                           PA3579_tr
                           translation of PA3579
                           494
                           99.39
                           99.59
                           0
                           2569.0
     

     
     
50698
                           gb|AAG06967.1|AE004778_11
                           (AE004778) probable carbohydrate kinase [Pseudomonas aeruginosa PAO1]
                           494
                           99.39
                           99.59
                           0
                           2569.0
     

     
     
50699
                           dbj|BAC46675.1|
                           (AP005940) glycerol kinase [Bradyrhizobium japonicum USDA 110]
                           501
                           61.87
                           74.25
                           8
                           1615.0
     

     
     
50701
                           emb|CAC49535.1|
                           (AL603646) probable glycerol kinase, similar to sugar kinases protein [Sinorhizobium meliloti]
                           497
                           60.56
                           74.04
                           3
                           1568.0
     

     
     
50700
                           emb|CAE29146.1|
                           (BX572604) glycerol kinase [Rhodopseudomonas palustris CGA009]
                           501
                           60.27
                           72.65
                           8
                           1581.0
     

     






      

          
GC ORFID: 43777How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3579Glimmer Score: 

          
Start: 3407933Stop: 3406449

          
Length: 1485


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3579 translation of PA3579Homolog Bit Score 994.0

          
Other Homologs:  t_PA3582 (ORF: BLASTX 547.0), psyr_15may02_Scaffold5_revised_gene6126 (ORF: BLASTX 537.0), t_PA3579 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCCACTATCTCCTCGCCATCGACCAGGGCACCACCAGCTCCCGCGCCATCGTGTTCAGTGCCCAGGGC 
TTGCCGGTGACGAGCTGCCAGCAGGAATTCAAGCAGTACTTCCCGAAGGACGGCTGGGTGGAGCACGACGGC 
GAGGAAATCTGGCTGACCACCCTCCAGGTCTGTCGCGACGCGCTCGCTCGCAAGGGCTTGCGCGCGGGCGAC 
ATCGCCGCCATCGGCATCACCAACCAGCGCGAGACCACGCTGGTCTGGGACGCCGCCAGCGGCGACCTGATC 
CACCCGGCCATCGTCTGGCAGGATCGTCGTACCGCCGACTACTGTGCCGAACTCAAGGCCGCCGGCCACGAG 
GCGAACGTCAGCGCGCGCACCGGGCTGCTGATCGATCCGTATTTCTCCGCCACCAAGCTGCGCTGGATCCTC 
GACAACGTGCCCGGCGCCCGCCAGCGCGCCGAACGCGGCGAGCTGCGCTTCGGCACCGTCGACTGCTTCCTG 
CTCTGGCGCCTGACCGGCGGCCGCTCCCATCGCACCGACGCGACCAACGCTTCGCGCACCCTGCTGTTCAAC 
ATCCACAGCCAGGACTGGGACGAGGAATTGCTGGCGCTGTTCGAGATCCCGCGCAGCCTGCTGCCGGAGGTA 
CTCGACTGCGCGGCGGAGTTCGGCGTCAGCGAGCCGTCGCTGCTTGGCGCGGCGATCCCGGTGCTGGGCATG 
GCCGGCGACCAGCAGGCCGCGCTGATCGGCCAGGCCTGCTTCCAGCCGGGCATGGTGAAGAGCACCTACGGC 
ACCGGCTGCTTCATGATCCAGAACACTGGCGAGCAGCCGGTGACCTCGAAGAACCGCCTGCTCACCACGGTT 
GGCTACCGTCTGGACGGCAAGGTCAGCTACGCGGTGGAGGGCAGCATCTTCGTCGCCGGCGCCGCGGTGCAA 
TGGCTGCGCGACGGCATCAAGCTGATCGACCACGCCCACGAGAGCGAAGCCCTGGCGATCCAGGCCGGCGAC 
TCCAACGGCGTCTACCTGGTGCCGGCCTTTACCGGCCTCGGCGCGCCCTACTGGGACCCGAAGGCGCGCGGC 
GCGATCTTCGGCCTGACCCGCGACACCGGAATCAAGGAGATCGTCACCGCCGGTCTGCAATCGGTCTGCTAC 
CAGACCCGCGACCTGCTCGAAGCGATGCGCCAGGACGGCACGCCGCCCAGCGCGCTGCGGGTGGACGGCGGG 
ATGGTGGTGAACAACTGGGTGATGCAATTTCTCACCGACATCCTTGGTGTCACCGTGGAGCGCCCGGAAGTC 
ACCGAGACCACCGCCCTCGGCGTGGCCTACATGGCCGGGCTGAAGGCAGGCTTCTATCGCGACCTGGACGAC 
ATCGCCAGCCACTGGCACCTGCAGCGCCGCTTCGCCGCACACATGGCCGAGGAGCGCCGCGGCGAACTCTAC 
GCCGGCTGGCAGAACGCGGTGCGACGGGTGCGCAGCGAGGCCTGA