ViewGene
Locus Name:
GeneID:
Location:

GeneID:77
TrivialName: PA14_60370
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60370
PAO1 Ortholog Locus:PA4560
Sequence Length:2832
Protein Length:943
Start: 5382246
Stop: 5379415
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07948.1|AE004869_12 (AE004869) isoleucyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
Identities = 940/943 (99%), Positives = 942/943 (99%)
Sequence:
ATGACCGACTACAAAGCGACGCTAAACCTTCCGGAAACCGCCTTCCCAATGAAAGCCGGCCTGCCCCAGCGC
GAGCCGGAAACCCTGAAGTTCTGGAACGACATCGGTCTGTACCAGAAGCTGCGGGCCATTGGCGGCGACCGT
CCGAAGTTCGTCCTGCACGATGGCCCTCCGTACGCCAACGGCAGCATTCACATCGGTCACGCGGTCAACAAG
ATTCTCAAGGACATCATTGTCCGCTCCAAGACCCTGGCGGGCTACGACGCGCCCTATGTGCCGGGCTGGGAT
TGCCACGGCCTGCCCATCGAGCACAAGGTCGAGACCACCCACGGCAAGAATCTTCCGGCGGACAAGACCCGC
GAACTGTGCCGCGAATACGCAGCCGAGCAGATCGAAGGGCAGAAGGCCGACTTCATCCGCCTGGGCGTGCTC
GGCGAGTGGGACAACCCCTACAAGACCATGAATTTCGCCAACGAGGCGAACGAAATCCGCGCCCTCGCGGAA
ATGGTCAAGCAGGACTTCGTGTTCAAGGGCCTGAAGCCGGTCAACTGGTGCTTCGACTGTGGTTCGGCGCTG
GCCGAGGCCGAGGTCGAGTACGCCGACAAGAAGTCGCCGACCATCGACGTCGGCTTCCCGGTGGCGGATGCC
GACAAGCTGGCCGCGGCCTTCGGCCTGGCGGCCCTGGACAAGCCGGCGCAGATCGTCATCTGGACCACCACG
CCCTGGACCATCCCGGCCAACCAGGCGCTGAACGTGCATCCGGAGATCGACTACGCGCTGGTCGATGCCGGC
GATCGCTACCTGGTGCTGGCCGAGGCCCTGGTCGAGTCCTGCCTGGCGCGCTACCAGCGCGAGGGCAAGGTG
GTCGCCACCGCCAAGGGCGAGGCGCTGGAACTGATCAACTTCCGCCATCCGTTCTACGAGCGCCTGTCGCCG
GTCTACCTGGCCGACTACGTGGCCCTCGATGCCGGCACCGGTATCGTCCACTCGTCGCCGGCCTACGGCGAA
GACGACTTCTATACCTGCAAGCGCTACGGGATGAGCAACGACGACATCCTCAGCCCGGTGCAGAGCAACGGC
GTCTACGTCGACTCGTTGCCGTTCTTCGGCGGCCAGTTCATCTGGAAGGCCAACCCTAACGTGGTGGCCAAG
CTGGAAGAGGTCGGCAGCCTGCTGGCCCACGAAACCATCAATCACAGCTACATGCACTGCTGGCGGCACAAG
ACGCCGCTGATCTATCGCGCCACCGCGCAGTGGTTCGTCGGCATGGACAAGCAGCCCAGGCAGGGTGCTTCC
CTGCGCGAGCGAGCGCTGGAAGCCATCACCCAGACCGAATTCGTCCCGGGCTGGGGCCAGGCGCGCCTGCAC
GGAATGATCGCCGGGCGTCCCGACTGGTGCATCTCGCGCCAGCGCAACTGGGGCGTACCGATCCCGTTCTTC
TTGCACAAGGCCAGCGGCGAACTGCATCCACGCACCGTCGAGCTGATGGAAGAGGTCGCGCAGCGAGTCGAG
AAGGAAGGCATCGAAGCCTGGTTCAAGCTCGACGCCGCCGAGTTGCTCGGCGAGGAGGCCGCGCAGTACGAC
AAGATCAACGACACCCTGGACGTCTGGTTCGACTCCGGCACCACCCACTGGCACGTGCTGCGTGGCTCCCAC
CGGATCGGCCATGCCAGCGGCCCGGTCGCCGATCTCTACCTGGAAGGTTCCGACCAGCACCGCGGCTGGTTC
CACTCCTCGCTGCTGACCGGCTGCGCGATCGACAACCATGCGCCGTACCGCCAGTTGCTGACCCACGGTTTC
ACCGTCGACGAGTCCGGCCGCAAGATGTCCAAGTCACTGGGCAATACCGTGGTGCCGCAGACCGTCATCGAT
ACCCTGGGCGCCGACATCCTGCGCCTGTGGGTAGCCTCGACCGATTACTCGGGCGAGATCGCGGTATCCCAG
CAGATTCTCCAGCGCAGCGCCGATGCCTACCGGCGGATCCGCAATACCACGCGCTTCCTGCTGTCCAACCTG
AACGGTTTCGATGCGGCCACTGATCTGCTGCCGCCCCAGGAAATGCTGGCCCTGGATCGCTGGGCGGTCGAT
CGTGCCCTGCTCTTGCAGCGCGAGATCGAAGAGGCGTACCGCGAGTACCGCTTCTGGAACGTCTATTCCAAG
GTGCACAACTTCTGCGTACAGGAGCTGGGCGGGTTCTACCTGGACATCATCAAGGACCGCCAGTACACCACC
GGCGCCAACAGCGTCGCCCGGCGCTCCTGCCAGACCGCACTGTTCCACATCGCCGAGGCCCTCGTACGCTGG
ATCGCCCCGATCCTGGCGTTCACCGCCGAGGAAGTCTGGAAGTTCCTGCCCGGCGAGCGCGCCGAGTCGGTA
ATGCTCGCCACCTGGTACGACGGCCTCAGCGAACTGCCGGCCGACGTGACGCTGAATCGCCAGTACTGGGAG
CAGGTCATGGCGGTGAAGGCCGCGGTCAACAAGGAGCTGGAGAACCAGCGCGCGGCCAAGGCCGTGGGCGGC
AACCTGCAGGCCGAAGTCACCCTGTATGCGGAAGACGCGCTGCAGGCCCAATTGGCCAAGCTGGGCAACGAG
CTACGCTTCGTCCTGATCACCTCGACCGCGACCCTGGCGCCGCTGTCGGCGGCTCCGGCCGACGCGGTGGAC
AGCGAAGTCGCCGGCCTCAAGCTGAAAGTGGTCAAGTCGACCCACGCCAAGTGCGGTCGTTGCTGGCACCAT
CGCGAGGACGTCGGTCAGCATGCTGCGCATCCTGACCTGTGTGGTCGCTGCATCGAGAACATCGAAGGCAGC
GGCGAGGTTCGCCACTATGCCTGA
Translation:
MTDYKATLNLPETAFPMKAGLPQREPETLKFWNDIGLYQKLRAIGGDRPKFVLHDGPPYANGSIHIGHAVNK
ILKDIIVRSKTLAGYDAPYVPGWDCHGLPIEHKVETTHGKNLPADKTRELCREYAAEQIEGQKADFIRLGVL
GEWDNPYKTMNFANEANEIRALAEMVKQDFVFKGLKPVNWCFDCGSALAEAEVEYADKKSPTIDVGFPVADA
DKLAAAFGLAALDKPAQIVIWTTTPWTIPANQALNVHPEIDYALVDAGDRYLVLAEALVESCLARYQREGKV
VATAKGEALELINFRHPFYERLSPVYLADYVALDAGTGIVHSSPAYGEDDFYTCKRYGMSNDDILSPVQSNG
VYVDSLPFFGGQFIWKANPNVVAKLEEVGSLLAHETINHSYMHCWRHKTPLIYRATAQWFVGMDKQPRQGAS
LRERALEAITQTEFVPGWGQARLHGMIAGRPDWCISRQRNWGVPIPFFLHKASGELHPRTVELMEEVAQRVE
KEGIEAWFKLDAAELLGEEAAQYDKINDTLDVWFDSGTTHWHVLRGSHRIGHASGPVADLYLEGSDQHRGWF
HSSLLTGCAIDNHAPYRQLLTHGFTVDESGRKMSKSLGNTVVPQTVIDTLGADILRLWVASTDYSGEIAVSQ
QILQRSADAYRRIRNTTRFLLSNLNGFDAATDLLPPQEMLALDRWAVDRALLLQREIEEAYREYRFWNVYSK
VHNFCVQELGGFYLDIIKDRQYTTGANSVARRSCQTALFHIAEALVRWIAPILAFTAEEVWKFLPGERAESV
MLATWYDGLSELPADVTLNRQYWEQVMAVKAAVNKELENQRAAKAVGGNLQAEVTLYAEDALQAQLAKLGNE
LRFVLITSTATLAPLSAAPADAVDSEVAGLKLKVVKSTHAKCGRCWHHREDVGQHAAHPDLCGRCIENIEGS
GEVRHYA*
AnnotationID:87GeneID:77
AnnotatorUID: diggins
Modification Date/Time: 2005-05-05 06:06:45
Gene Name:ileS
Confidence Code:2
GeneProduct:isoleucyl-tRNA synthetase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:KOG0433,COG0060
Secondary Functional Category(ies):
EC Number:6.1.1.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66229.1|AE016251_5 (AE016776) isoleucyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 943

 Score = 1644 bits (4258), Expect = 0.0
 Identities = 783/943 (83%), Positives = 853/943 (90%)

>gb|AAN78530.1|AE016755_30 (AE016755) Isoleucyl-tRNA synthetase [Escherichia coli CFT073]
          Length = 938

 Score = 1092 bits (2825), Expect = 0.0
 Identities = 541/949 (57%), Positives = 690/949 (72%), Gaps = 17/949 (1%)








 
Structural Features:
KOG0433, Isoleucyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 937 residues,  97.5% aligned
Score =  705 bits (1820), Expect = 0.0
Identities = 316/957 (33%), Positives = 470/957 (49%), Gaps = 72/957 (7%)

COG0060, IleS, Isoleucyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 933 residues, 100.0% aligned
Score = 1244 bits (3221), Expect = 0.0
Identities = 470/955 (49%), Positives = 612/955 (64%), Gaps = 41/955 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:157




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17299




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31653




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:77_cdd.html


    		




 
URL:

















    ReferenceID:33020




  
Author/Investigator(s):
        Yanagisawa T, Lee JT, Wu HC, Kawakami M.





  
Title:
        Relationship of protein structure of isoleucyl-tRNA synthetase with pseudomonic acid resistance of Escherichia coli. A proposed mode of action of pseudomonic acid as an inhibitor of isoleucyl-tRNA synthetase.




  
PubMed:
        7929087


MedLine:
        




    
Source:
J Biol Chem. 1994 Sep 30;269(39):24304-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7929087

















    ReferenceID:33021




  
Author/Investigator(s):
        Chalker AF, Ward JM, Fosberry AP, Hodgson JE.





  
Title:
        Analysis and toxic overexpression in Escherichia coli of a staphylococcal gene encoding isoleucyl-tRNA synthetase.




  
PubMed:
        8163160


MedLine:
        




    
Source:
Gene. 1994 Apr 8;141(1):103-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8163160

















    ReferenceID:33022




  
Author/Investigator(s):
        Masuda Y, Tsuchimoto S, Nishimura A, Ohtsubo E.





  
Title:
        Isolation of temperature-sensitive aminoacyl-tRNA synthetase mutants from an Escherichia coli strain harboring the pemK plasmid.




  
PubMed:
        8479423


MedLine:
        




    
Source:
Mol Gen Genet. 1993 Apr;238(1-2):169-76.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8479423

















    ReferenceID:33023




  
Author/Investigator(s):
        Miller KW, Bouvier J, Stragier P, Wu HC.





  
Title:
        Identification of the genes in the Escherichia coli ileS-lsp operon. Analysis of multiple polycistronic mRNAs made in vivo.




  
PubMed:
        3294831


MedLine:
        




    
Source:
J Biol Chem. 1987 May 25;262(15):7391-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3294831

















    ReferenceID:33024




  
Author/Investigator(s):
        Miller KW, Wu HC.





  
Title:
        Cotranscription of the Escherichia coli isoleucyl-tRNA synthetase (ileS) and prolipoprotein signal peptidase (lsp) genes. Fine-structure mapping of the lsp internal promoter.




  
PubMed:
        2432063


MedLine:
        




    
Source:
J Biol Chem. 1987 Jan 5;262(1):389-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2432063

















    ReferenceID:33025




  
Author/Investigator(s):
        Kamio Y, Lin CK, Regue M, Wu HC.





  
Title:
        Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon.




  
PubMed:
        2985604


MedLine:
        




    
Source:
J Biol Chem. 1985 May 10;260(9):5616-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2985604









           
Homologs By Global Alignment 

           

       



     
Gene ID:77
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
154
                           PA4560_tr
                           translation of PA4560
                           943
                           99.68
                           99.89
                           0
                           4997.0
     

     
     
13529
                           gb|AAG07948.1|AE004869_12
                           (AE004869) isoleucyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           943
                           99.68
                           99.89
                           0
                           4997.0
     

     
     
13530
                           gb|AAN66229.1|AE016251_5
                           (AE016776) isoleucyl-tRNA synthetase [Pseudomonas putida KT2440]
                           943
                           83.03
                           90.45
                           0
                           4258.0
     

     
     
13531
                           gb|AAO54348.1|
                           (AE016858) isoleucyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           943
                           81.65
                           90.56
                           0
                           4199.0
     

     
     
13532
                           emb|CAA56431.1|
                           (X80132) isoleucine-tRNA ligase [Pseudomonas fluorescens]
                           943
                           80.69
                           88.65
                           0
                           4112.0
     

     






      

          
GC ORFID: 43079How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4560Glimmer Score: 

          
Start: 4645925Stop: 4643094

          
Length: 2832


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4560 translation of PA4560Homolog Bit Score 1929.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3534 (ORF: BLASTX 1625.0), t_PA4560 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGACTACAAAGCGACGCTAAACCTTCCGGAAACCGCCTTCCCAATGAAAGCCGGCCTGCCCCAGCGC 
GAGCCGGAAACCCTGAAGTTCTGGAACGACATCGGTCTGTACCAGAAGCTGCGGGCCATTGGCGGCGACCGT 
CCGAAGTTCGTCCTGCACGATGGCCCTCCGTACGCCAACGGCAGCATTCACATCGGTCACGCGGTCAACAAG 
ATTCTCAAGGACATCATTGTCCGCTCCAAGACCCTGGCGGGCTACGACGCGCCCTATGTGCCGGGCTGGGAT 
TGCCACGGCCTGCCCATCGAGCACAAGGTCGAGACCACCCACGGCAAGAATCTTCCGGCGGACAAGACCCGC 
GAACTGTGCCGCGAATACGCAGCCGAGCAGATCGAAGGGCAGAAGGCCGACTTCATCCGCCTGGGCGTGCTC 
GGCGAGTGGGACAACCCCTACAAGACCATGAATTTCGCCAACGAGGCGAACGAAATCCGCGCCCTCGCGGAA 
ATGGTCAAGCAGGACTTCGTGTTCAAGGGCCTGAAGCCGGTCAACTGGTGCTTCGACTGTGGTTCGGCGCTG 
GCCGAGGCCGAGGTCGAGTACGCCGACAAGAAGTCGCCGACCATCGACGTCGGCTTCCCGGTGGCGGATGCC 
GACAAGCTGGCCGCGGCCTTCGGCCTGGCGGCCCTGGACAAGCCGGCGCAGATCGTCATCTGGACCACCACG 
CCCTGGACCATCCCGGCCAACCAGGCGCTGAACGTGCATCCGGAGATCGACTACGCGCTGGTCGATGCCGGC 
GATCGCTACCTGGTGCTGGCCGAGGCCCTGGTCGAGTCCTGCCTGGCGCGCTACCAGCGCGAGGGCAAGGTG 
GTCGCCACCGCCAAGGGCGAGGCGCTGGAACTGATCAACTTCCGCCATCCGTTCTACGAGCGCCTGTCGCCG 
GTCTACCTGGCCGACTACGTGGCCCTCGATGCCGGCACCGGTATCGTCCACTCGTCGCCGGCCTACGGCGAA 
GACGACTTCTATACCTGCAAGCGCTACGGGATGAGCAACGACGACATCCTCAGCCCGGTGCAGAGCAACGGC 
GTCTACGTCGACTCGTTGCCGTTCTTCGGCGGCCAGTTCATCTGGAAGGCCAACCCTAACGTGGTGGCCAAG 
CTGGAAGAGGTCGGCAGCCTGCTGGCCCACGAAACCATCAATCACAGCTACATGCACTGCTGGCGGCACAAG 
ACGCCGCTGATCTATCGCGCCACCGCGCAGTGGTTCGTCGGCATGGACAAGCAGCCCAGGCAGGGTGCTTCC 
CTGCGCGAGCGAGCGCTGGAAGCCATCACCCAGACCGAATTCGTCCCGGGCTGGGGCCAGGCGCGCCTGCAC 
GGAATGATCGCCGGGCGTCCCGACTGGTGCATCTCGCGCCAGCGCAACTGGGGCGTACCGATCCCGTTCTTC 
TTGCACAAGGCCAGCGGCGAACTGCATCCACGCACCGTCGAGCTGATGGAAGAGGTCGCGCAGCGAGTCGAG 
AAGGAAGGCATCGAAGCCTGGTTCAAGCTCGACGCCGCCGAGTTGCTCGGCGAGGAGGCCGCGCAGTACGAC 
AAGATCAACGACACCCTGGACGTCTGGTTCGACTCCGGCACCACCCACTGGCACGTGCTGCGTGGCTCCCAC 
CGGATCGGCCATGCCAGCGGCCCGGTCGCCGATCTCTACCTGGAAGGTTCCGACCAGCACCGCGGCTGGTTC 
CACTCCTCGCTGCTGACCGGCTGCGCGATCGACAACCATGCGCCGTACCGCCAGTTGCTGACCCACGGTTTC 
ACCGTCGACGAGTCCGGCCGCAAGATGTCCAAGTCACTGGGCAATACCGTGGTGCCGCAGACCGTCATCGAT 
ACCCTGGGCGCCGACATCCTGCGCCTGTGGGTAGCCTCGACCGATTACTCGGGCGAGATCGCGGTATCCCAG 
CAGATTCTCCAGCGCAGCGCCGATGCCTACCGGCGGATCCGCAATACCACGCGCTTCCTGCTGTCCAACCTG 
AACGGTTTCGATGCGGCCACTGATCTGCTGCCGCCCCAGGAAATGCTGGCCCTGGATCGCTGGGCGGTCGAT 
CGTGCCCTGCTCTTGCAGCGCGAGATCGAAGAGGCGTACCGCGAGTACCGCTTCTGGAACGTCTATTCCAAG 
GTGCACAACTTCTGCGTACAGGAGCTGGGCGGGTTCTACCTGGACATCATCAAGGACCGCCAGTACACCACC 
GGCGCCAACAGCGTCGCCCGGCGCTCCTGCCAGACCGCACTGTTCCACATCGCCGAGGCCCTCGTACGCTGG 
ATCGCCCCGATCCTGGCGTTCACCGCCGAGGAAGTCTGGAAGTTCCTGCCCGGCGAGCGCGCCGAGTCGGTA 
ATGCTCGCCACCTGGTACGACGGCCTCAGCGAACTGCCGGCCGACGTGACGCTGAATCGCCAGTACTGGGAG 
CAGGTCATGGCGGTGAAGGCCGCGGTCAACAAGGAGCTGGAGAACCAGCGCGCGGCCAAGGCCGTGGGCGGC 
AACCTGCAGGCCGAAGTCACCCTGTATGCGGAAGACGCGCTGCAGGCCCAATTGGCCAAGCTGGGCAACGAG 
CTACGCTTCGTCCTGATCACCTCGACCGCGACCCTGGCGCCGCTGTCGGCGGCTCCGGCCGACGCGGTGGAC 
AGCGAAGTCGCCGGCCTCAAGCTGAAAGTGGTCAAGTCGACCCACGCCAAGTGCGGTCGTTGCTGGCACCAT 
CGCGAGGACGTCGGTCAGCATGCTGCGCATCCTGACCTGTGTGGTCGCTGCATCGAGAACATCGAAGGCAGC 
GGCGAGGTTCGCCACTATGCCTGA