ViewGene

Locus Name:
GeneID:
Location:

GeneID: 768

TrivialName: PA14_18120

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_18120

PAO1 Ortholog Locus: PA3570

Sequence Length: 1494

Protein Length: 497

Start: 1554529

Stop: 1556022

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG06958.1|AE004778_2 (AE004778) methylmalonate-semialdehyde dehydrogenase [Pseudomonas aeruginosa PAO1] Identities = 496/497 (99%), Positives = 497/497 (100%)

Sequence:

ATGTCAGTCCCAGTCCGCCACCTCATCGCGGGAGCCTTCGTCGAAGGTCTCGGCGCCCAGCGCATCCCGGTC
AGCAATCCCCTCGACAACAGCACCCTGGCGGAGATCGCCTGCGCCAGCGCGGAGCAGATCGAGCAGGCGGTG
GCCAGTGCCCGCGAAACCTTCGCCAGCTGGAAGGAAACCCCGGTTTCCGAGCGCGCGCGGGTGATGCTGCGT
TACCAGGCGTTGCTCAAGGAACACCACGACGAACTGGCGAAGATCGTCTCCAGCGAGCTGGGCAAGACTTTC
GAGGACGCCAAGGGCGACGTCTGGCGCGGTATCGAGGTGGTCGAGCACGCCTGCAACGTGCCCTCGCTGCTG
ATGGGCGAGACGGTGGAGAACGTCGCCCGCAACATCGACACCTACAGCATCACCCAGCCGCTGGGCGTGTGC
GTTGGCATCACCCCGTTCAACTTCCCGGCGATGATCCCGCTGTGGATGTTCCCGCTGGCCATCGCCTGCGGC
AACGCCTTCATCCTCAAGCCTTCCGAGCAGGTGCCGCTGACCAGCGTGCGGCTCGCCGAGCTGTTCCTCGAG
GCCGGTGCGCCGAAGGGCGTGCTGCAGGTGGTGCATGGCGGCAAGGAACAGGTCGACCAGTTGCTCAAGCAC
CCGCAGGTGAAGGCGGTGTCCTTCGTCGGCTCGGTCGCCGTCGGCCAGTACGTCTACCACACCGGCACCGCG
CACAATAAGCGCGTGCAGAGCTTCGCCGGGGCGAAGAACCACATGGTGATCATGCCCGACGCGGACAAGGCA
CAGGTCATCAGCAACCTGGTCGGTGCCTCGGTGGGCGCGGCCGGGCAGCGCTGCATGGCGATCTCGGTGGCG
GTGCTGGTGGGGGCCGCCCGCGAGTGGATTCCGGAAATCCGCGACGCGCTGGCCAAGGTCCGCCCCGGCCCA
TGGGACGACAGCGGCGCCAGCTACGGCCCGGTGATCAACCCGCAGGCCAAGGCGCGCATCGAGCGTCTGATC
GGCCAGGGCGTGGAGGAGGGCGCGCAACTGCTGCTGGATGGCCGCGGCTACAAGGTCGAGGGATACCCGGAC
GGCAACTGGGTCGGCCCGACGCTGTTCGCCGGGGTGCGCCCGGACATGGCGATCTACCGCGAAGAGGTGTTC
GGCCCGGTGCTCTGCCTGGCCGAGGTCGACAGCCTGGAGCAGGCGATCCGCCTGATCAACGAAAGCCCCTAC
GGCAACGGCACCTCGATCTTCACCAGCTCCGGCGCCGCGGCGCGGACCTTCCAGCACCACATCGAGGTCGGC
CAGGTCGGCATCAACATCCCGATTCCGGTACCGCTGCCGTTCTTCTCCTTCACCGGTTGGAAGGGCTCGTTC
TACGGCGACCTGCACGCCTACGGCAAGCAGGGCGTGCGCTTCTACACCGAGACCAAGACCGTGACCGCGCGC
TGGTTCGACAGCGATAGCGTGGCCGGGACCAACTTCTCGATCCAGATGCGCTGA

Translation:

MSVPVRHLIAGAFVEGLGAQRIPVSNPLDNSTLAEIACASAEQIEQAVASARETFASWKETPVSERARVMLR
YQALLKEHHDELAKIVSSELGKTFEDAKGDVWRGIEVVEHACNVPSLLMGETVENVARNIDTYSITQPLGVC
VGITPFNFPAMIPLWMFPLAIACGNAFILKPSEQVPLTSVRLAELFLEAGAPKGVLQVVHGGKEQVDQLLKH
PQVKAVSFVGSVAVGQYVYHTGTAHNKRVQSFAGAKNHMVIMPDADKAQVISNLVGASVGAAGQRCMAISVA
VLVGAAREWIPEIRDALAKVRPGPWDDSGASYGPVINPQAKARIERLIGQGVEEGAQLLLDGRGYKVEGYPD
GNWVGPTLFAGVRPDMAIYREEVFGPVLCLAEVDSLEQAIRLINESPYGNGTSIFTSSGAAARTFQHHIEVG
QVGINIPIPVPLPFFSFTGWKGSFYGDLHAYGKQGVRFYTETKTVTARWFDSDSVAGTNFSIQMR*

AnnotationID:777 GeneID:768

AnnotatorUID: diggins

Modification Date/Time: 2004-04-30 13:01:11

Gene Name: mmsA

Confidence Code: 1

GeneProduct: methylmalonate-semialdehyde dehydrogenase

Cell Localization: ()

Synonyms:

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (2) Amino acid biosynthesis and metabolism

Alternate Gene Product Name: Aldehyde dehydrogenase

Functional Category Confidence Code: 5

COGs: pfam00171,COG1012,KOG2450

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Homology:

>gb|AAN54733.1|AE015613_10 (AE015613) methylmalonate-semialdehyde dehydrogenase [Shewanella
           oneidensis MR-1]
          Length = 499

 Score =  750 bits (1937), Expect = 0.0
 Identities = 366/499 (73%), Positives = 415/499 (83%), Gaps = 2/499 (0%)

>dbj|BAC61964.1| (AP005086) methylmalonate-semialdehyde dehydrogenase [Vibrio
           parahaemolyticus]
          Length = 497

 Score =  688 bits (1776), Expect = 0.0
 Identities = 329/489 (67%), Positives = 398/489 (81%)

Structural Features:

pfam00171, Aldedh, Aldehyde dehydrogenase family.

COG1012, PutA, NAD-dependent aldehyde dehydrogenases [Energy production and conversion]

KOG2450, Aldehyde dehydrogenase [Energy production and conversion]

Genomic Context:

Comment:

ReferenceID:1539

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:18681

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:31970

Author/Investigator(s):

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: Misc (text/plain)

Data: 768_cdd.html

URL:

ReferenceID:34155

Author/Investigator(s): Steele MI, Lorenz D, Hatter K, Park A, Sokatch JR.

Title: Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase.

PubMed: 1339433

MedLine:

Source:

J Biol Chem. 1992 Jul 5;267(19):13585-92.

Reference Type: Journal

Data:

URL:	`http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1339433`

Homologs By Global Alignment

Gene ID:768

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

1349 PA3570_tr translation of PA3570 497 99.79 99.99 0 2574.0

49844 gb|AAG06958.1|AE004778_2 (AE004778) methylmalonate-semialdehyde dehydrogenase [Pseudomonas aeruginosa PAO1] 497 99.79 99.99 0 2574.0

49845 gb|AAA25891.1| (M84911) methylmalonate semialdehyde dehydrogenase [Pseudomonas aeruginosa] 497 99.79 99.99 0 2574.0

49846 gb|AAN54733.1|AE015613_10 (AE015613) methylmalonate-semialdehyde dehydrogenase [Shewanella oneidensis MR-1] 499 73.34 83.16 2 1939.0

1350 PA0747_tr translation of PA0747 502 68.52 80.67 5 1834.0

GC ORFID: `43770`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA3570`	Glimmer Score:
Start: `3399407`	Stop: `3397914`
Length: `1494`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA3570 translation of PA3570`	Homolog Bit Score `996.0`
Other Homologs: `t_PA0747 (ORF: BLASTX 709.0), psyr_15may02_Scaffold2_revised_gene3514 (ORF: BLASTX 577.0), t_PA0130 (ORF: BLASTX 499.0), psyr_15may02_Scaffold2_revised_gene3505 (ORF: BLASTX 492.0), psyr_15may02_Scaffold2_revised_gene3039 (ORF: BLASTX 486.0), t_PA3570 (ORF: GLIMMER)`
GC ORF Sequence
ATGTCAGTCCCAGTCCGCCACCTCATCGCGGGAGCCTTCGTCGAAGGTCTCGGCGCCCAGCGCATCCCGGTC AGCAATCCCCTCGACAACAGCACCCTGGCGGAGATCGCCTGCGCCAGCGCGGAGCAGATCGAGCAGGCGGTG GCCAGTGCCCGCGAAACCTTCGCCAGCTGGAAGGAAACCCCGGTTTCCGAGCGCGCGCGGGTGATGCTGCGT TACCAGGCGTTGCTCAAGGAACACCACGACGAACTGGCGAAGATCGTCTCCAGCGAGCTGGGCAAGACTTTC GAGGACGCCAAGGGCGACGTCTGGCGCGGTATCGAGGTGGTCGAGCACGCCTGCAACGTGCCCTCGCTGCTG ATGGGCGAGACGGTGGAGAACGTCGCCCGCAACATCGACACCTACAGCATCACCCAGCCGCTGGGCGTGTGC GTTGGCATCACCCCGTTCAACTTCCCGGCGATGATCCCGCTGTGGATGTTCCCGCTGGCCATCGCCTGCGGC AACGCCTTCATCCTCAAGCCTTCCGAGCAGGTGCCGCTGACCAGCGTGCGGCTCGCCGAGCTGTTCCTCGAG GCCGGTGCGCCGAAGGGCGTGCTGCAGGTGGTGCATGGCGGCAAGGAACAGGTCGACCAGTTGCTCAAGCAC CCGCAGGTGAAGGCGGTGTCCTTCGTCGGCTCGGTCGCCGTCGGCCAGTACGTCTACCACACCGGCACCGCG CACAATAAGCGCGTGCAGAGCTTCGCCGGGGCGAAGAACCACATGGTGATCATGCCCGACGCGGACAAGGCA CAGGTCATCAGCAACCTGGTCGGTGCCTCGGTGGGCGCGGCCGGGCAGCGCTGCATGGCGATCTCGGTGGCG GTGCTGGTGGGGGCCGCCCGCGAGTGGATTCCGGAAATCCGCGACGCGCTGGCCAAGGTCCGCCCCGGCCCA TGGGACGACAGCGGCGCCAGCTACGGCCCGGTGATCAACCCGCAGGCCAAGGCGCGCATCGAGCGTCTGATC GGCCAGGGCGTGGAGGAGGGCGCGCAACTGCTGCTGGATGGCCGCGGCTACAAGGTCGAGGGATACCCGGAC GGCAACTGGGTCGGCCCGACGCTGTTCGCCGGGGTGCGCCCGGACATGGCGATCTACCGCGAAGAGGTGTTC GGCCCGGTGCTCTGCCTGGCCGAGGTCGACAGCCTGGAGCAGGCGATCCGCCTGATCAACGAAAGCCCCTAC GGCAACGGCACCTCGATCTTCACCAGCTCCGGCGCCGCGGCGCGGACCTTCCAGCACCACATCGAGGTCGGC CAGGTCGGCATCAACATCCCGATTCCGGTACCGCTGCCGTTCTTCTCCTTCACCGGTTGGAAGGGCTCGTTC TACGGCGACCTGCACGCCTACGGCAAGCAGGGCGTGCGCTTCTACACCGAGACCAAGACCGTGACCGCGCGC TGGTTCGACAGCGATAGCGTGGCCGGGACCAACTTCTCGATCCAGATGCGCTGA