ViewGene
Locus Name:
GeneID:
Location:

GeneID:76
TrivialName: PA14_56890
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56890
PAO1 Ortholog Locus:PA4375
Sequence Length:3057
Protein Length:1018
Start: 5068199
Stop: 5071255
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07763.1|AE004853_6 (AE004853) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
Identities = 1017/1018 (99%), Positives = 1018/1018 (100%)
Sequence:
ATGGCTTTTACCGATCCGTTCATCCGTCGTCCGGTCCTGGCGAGCGTGGTCAGCCTGCTGATCGTCCTGCTC
GGCATGCAGGCCTTCAGCAAGCTGGTGATCCGCGAGTATCCGCAAATGGAGAACGCGCTGATCACGGTGACC
ACGCTCTACGCCGGAGCCAACGCGGAGACCATCCAGGGCTACATCACCCAGCCGCTGCAGCAGAGCCTGGCC
AGCGCCGAAGGCATCGACTACATGACCTCGGTGAGCCGGCAGAACTATTCGACCATCTCCATCTACGCGCGG
ATCGGCGCCAATACCGATCGCCTGGTCACCGAGCTGCTGGCCAAGTCCAACGAAGTGAAGAGCCAACTGCCG
CCGGACGCCGAGGACCCGGTGCTGCAGAAGGAGGCCGCGGATGCCTCGGCGCTGATGTACATCAGCTTCTAC
AGCGAGCAGATGAACAACCCGCAGATCACCGACTACCTGTCGCGGGTGATCCAGCCCAAGCTGGCGACCCTG
CCCGGTATCGCCGAGGCGGAGATCCTCGGCAACCAGGTGTTCGCCATGCGCCTGTGGCTGGACCCGGTGAAG
ATGGCCGCGTTCGGCGTCACCGCCGGCGAGATCAACCAGGCGGTGCAGCAGTACAACTTCCTCGCCGCCGCC
GGCGAGGTGAAGGGCCAGTTGGTGGTCACCAGCGTCAATGCTTCCACCGACCTCAAGTCGCCCCAGGCCTTC
GCCGCCATCCCGGTGAAGACCGACGGCGACCGCCGGGTGCTGATGGGCGATGTCGCACGGGTCGAACTGGGC
GCCGCCAGCTACGACGCGATCAGTTCGTTCAATGGGATTCCCTCGGTCTACATCGGCATCAAGGGCACGCCC
AGCGCCAACCCGCTGGACGTGATCAAGGAAGTGCGGGCGAAGATGCCCGAACTGGAAGAGCAATTGCCGCCC
AACCTCAAGGTGTCCATCGCCTACGACGCCACGCGCTTCATCCAGGCCTCCATCGACGAAGTGGTGAAGACC
CTCGGCGAGGCGGTGCTGATCGTCATCGTGGTGGTGTTCCTGTTCCTCGGCGCGTTCCGTTCGGTACTGATC
CCGGTGGTGACCATTCCGCTGTCGATGATCGGCGTGTTGTTCTTCATGCAGGCCATGGGCTACTCGATCAAC
CTGCTGACCCTGCTGGCGATGGTCCTGGCCATCGGCCTGGTGGTGGACGACGCGATCGTGGTGGTGGAAAAC
ATCCACCGCCACATCGAGGAGGGCAAGCCGCCCTTCGAGGCCGCCCTGGAGGGCGCGCGGGAGATCGCCGTG
CCGGTGGTCAGCATGACCATCACCCTCGCCGCGGTCTACGCGCCGATCGGCTTTCTCACCGGCCTCACCGGC
GCCCTGTTCAAGGAATTCGCCTTCACCCTGGCCGGCGCGGTGATCATTTCCGGGATCGTCGCCCTGACCCTG
TCGCCGATGATGTGCTCGCGCCTGTTGCGCCACGAGGAGAATCCCTCGGGCCTGGCGCATCGCCTCGACCTG
ATCTTCGAGGGCCTGAAGCGACGCTACCAGCGCGCCCTCCACGGCACCCTGGACACCCGTCCGGTGGTCCTG
GTGTTCGCCGTGCTGGTACTGGCGCTGATCCCGGTACTGCTGATGTTCACCAAGAAGGAGCTGGCGCCGGAA
GAGGACCAGGGCATCGTGTTCCTCATGACCAACTCGCCGCAGACCGCCAACCTCGACTACCTGAATCGCTAT
ACCGCCGAGTTCGAAGGCATCTTCCGCAGCTTCCCGGAGTATTACTCGGCGTTCCAGATCAACGGCTACAAC
GGCGTCCAGGCCGGCATCGGCGGCATGCTGCTCAAGCCCTGGGACGAACGCGAAAAGAGCCAGATGGAATTG
CTCCATGCGGTGCAGGCGAAGCTCAACGAGATTCCCGGCGTGCAGATCTTCGCCTTCAACCTGCCGTCGCTA
CCGGGCACCGGCGAGGGCCTGCCATTCCAGTTCGTGCTCAACACCGCCAACGACTACGAGTCGCTGCTGCAA
GTGGCGCAACGGGTGAAGCAGCGCGCCAGCGAGTCCGGCAAGTTCGCCTTCCTCGATCTCGACCTGGCGTTC
GACAAACCCGAGCTGGTGGTCGACATCGACCGCGAGAAGGCCGCGCAGATGGGCGTCTCCATGCAGGACCTG
GGGGTGGCGCTGGCCAGCCTGCTCGGCGAGGGCGAGATCAACCGCTTCACCATCGACGGGCGCAGCTACAAG
GTCATCGCCCAGGTCGAACGCCCCTATCGCGACAATCCCGGCTGGCTCGGCAGCTACTACGTGAAGAGCCGC
AACGGCCAACTGGTCGCCCTCTCGACCCTGATCGAGACCCACGAACGGGCACGGCCGCGGCAACTGAACCAG
TTCCAGCAGCTCAACTCGGCGATCATCTCCGGCTTCCCCATCGTCAGCATGGGCGAGGCCATCGAGACGGTT
CAGCAGATCGCCCGCGAGGAGGCCCCGCGCGGCTTCGCCGTCGACTACGCCGGCGCTTCCCGGCAATACGTG
CAGGAAGGCAGCGCATTGCTGGTCACCTTCGGACTGGCCCTGGCGATCATCTTCCTGGTCCTCGCCGCACAG
TTCGAAAGCTTCCGCGATCCGCTGGTGATCATGGTCACCGTGCCGCTGTCGATCTGCGGTGCGCTGATACCG
CTGTTCCTCGGCGTGTCGAGCCTGAACATCTATACCCAGGTCGGCCTGGTGACACTGATCGGCCTGATCAGC
AAACACGGCATCCTGATCGTCGAGTTCGCCAACCAGTTGCGCCACGAACAGGGGCTGGGGCGTCGCGAGGCC
ATCGAGCAGGCCGCCGCGATCCGCCTGCGGCCGGTACTGATGACCACCGCGGCGATGGTGCTCGGGGTGATC
CCACTGATCCTCGCCACCGGCGCCGGGGCGGTCAGTCGCTTCGACATCGGCATCGTGATCGCTACCGGCATG
TCGGTGGGCACCCTCTTCACCCTGTTCGTCCTGCCGTGCATCTACACCCTGGTGGCCAGGCCGGATGCCCCG
CCAgGCGTGACGCAAGCGGCAAACGCACACTGA
Translation:
MAFTDPFIRRPVLASVVSLLIVLLGMQAFSKLVIREYPQMENALITVTTLYAGANAETIQGYITQPLQQSLA
SAEGIDYMTSVSRQNYSTISIYARIGANTDRLVTELLAKSNEVKSQLPPDAEDPVLQKEAADASALMYISFY
SEQMNNPQITDYLSRVIQPKLATLPGIAEAEILGNQVFAMRLWLDPVKMAAFGVTAGEINQAVQQYNFLAAA
GEVKGQLVVTSVNASTDLKSPQAFAAIPVKTDGDRRVLMGDVARVELGAASYDAISSFNGIPSVYIGIKGTP
SANPLDVIKEVRAKMPELEEQLPPNLKVSIAYDATRFIQASIDEVVKTLGEAVLIVIVVVFLFLGAFRSVLI
PVVTIPLSMIGVLFFMQAMGYSINLLTLLAMVLAIGLVVDDAIVVVENIHRHIEEGKPPFEAALEGAREIAV
PVVSMTITLAAVYAPIGFLTGLTGALFKEFAFTLAGAVIISGIVALTLSPMMCSRLLRHEENPSGLAHRLDL
IFEGLKRRYQRALHGTLDTRPVVLVFAVLVLALIPVLLMFTKKELAPEEDQGIVFLMTNSPQTANLDYLNRY
TAEFEGIFRSFPEYYSAFQINGYNGVQAGIGGMLLKPWDEREKSQMELLHAVQAKLNEIPGVQIFAFNLPSL
PGTGEGLPFQFVLNTANDYESLLQVAQRVKQRASESGKFAFLDLDLAFDKPELVVDIDREKAAQMGVSMQDL
GVALASLLGEGEINRFTIDGRSYKVIAQVERPYRDNPGWLGSYYVKSRNGQLVALSTLIETHERARPRQLNQ
FQQLNSAIISGFPIVSMGEAIETVQQIAREEAPRGFAVDYAGASRQYVQEGSALLVTFGLALAIIFLVLAAQ
FESFRDPLVIMVTVPLSICGALIPLFLGVSSLNIYTQVGLVTLIGLISKHGILIVEFANQLRHEQGLGRREA
IEQAAAIRLRPVLMTTAAMVLGVIPLILATGAGAVSRFDIGIVIATGMSVGTLFTLFVLPCIYTLVARPDAP
PGVTQAANAH*
AnnotationID:86GeneID:76
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 07:07:27
Gene Name:
Confidence Code:3
GeneProduct:putative RND efflux transporter
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00873,COG0841,COG3696
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66531.1|AE016281_12 (AE016777) multidrug efflux RND transporter, putative [Pseudomonas
            putida KT2440]
          Length = 1014

 Score = 1618 bits (4191), Expect = 0.0
 Identities = 828/1003 (82%), Positives = 921/1003 (91%)

>gb|AAO90606.1| (AE016963) transporter, AcrB/AcrD/AcrF family [Coxiella burnetii RSA
            493]
          Length = 1019

 Score = 1051 bits (2718), Expect = 0.0
 Identities = 540/1008 (53%), Positives = 730/1008 (72%), Gaps = 2/1008 (0%)

>emb|CAE35827.1| (BX640448) integral membrane component of multidrug efflux system
            [Bordetella bronchiseptica]
          Length = 1037

 Score =  996 bits (2574), Expect = 0.0
 Identities = 523/1017 (51%), Positives = 718/1017 (70%), Gaps = 3/1017 (0%)






 
Structural Features:
pfam00873, ACR_tran, AcrB/AcrD/AcrF family.
CD-Length = 1017 residues, 100.0% aligned
Score =  823 bits (2128), Expect = 0.0
Identities = 393/1018 (38%), Positives = 589/1018 (57%), Gaps = 18/1018 (1%)

COG0841, AcrB, Cation/multidrug efflux pump [Defense mechanisms]
CD-Length = 1009 residues,  99.8% aligned
Score =  921 bits (2382), Expect = 0.0
Identities = 416/1011 (41%), Positives = 626/1011 (61%), Gaps = 13/1011 (1%)

COG3696, Putative silver efflux pump 
[Inorganic ion transport and metabolism]
CD-Length = 1027 residues,  98.9% aligned
Score =  463 bits (1194), Expect = 7e-131
Identities = 283/1037 (27%), Positives = 502/1037 (48%), Gaps = 56/1037 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:155




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17133




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:76_cdd.html


    		




 
URL:

















    ReferenceID:17297




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:76
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
152
                           PA4375_tr
                           translation of PA4375
                           1018
                           99.90
                           99.99
                           0
                           5037.0
     

     
     
13504
                           gb|AAG07763.1|AE004853_6
                           (AE004853) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
                           1018
                           99.90
                           99.99
                           0
                           5037.0
     

     
     
13505
                           gb|AAN66531.1|AE016281_12
                           (AE016777) multidrug efflux RND transporter, putative [Pseudomonas putida KT2440]
                           1020
                           81.27
                           90.58
                           8
                           4187.0
     

     
     
13506
                           dbj|BAC48126.1|
                           (AP005945) blr2861 [Bradyrhizobium japonicum USDA 110]
                           1038
                           54.14
                           72.54
                           27
                           2812.0
     

     
     
13507
                           emb|CAE29216.1|
                           (BX572605) putative RND efflux transporter [Rhodopseudomonas palustris CGA009]
                           1042
                           53.45
                           71.49
                           32
                           2797.0
     

     






      

          
GC ORFID: 43078How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4375Glimmer Score: 

          
Start: 4331875Stop: 4334931

          
Length: 3057


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4375 translation of PA4375Homolog Bit Score 1944.0

          
Other Homologs:  t_PA4207 (ORF: BLASTX 984.0), t_PA4375 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTTTTACCGATCCGTTCATCCGTCGTCCGGTCCTGGCGAGCGTGGTCAGCCTGCTGATCGTCCTGCTC 
GGCATGCAGGCCTTCAGCAAGCTGGTGATCCGCGAGTATCCGCAAATGGAGAACGCGCTGATCACGGTGACC 
ACGCTCTACGCCGGAGCCAACGCGGAGACCATCCAGGGCTACATCACCCAGCCGCTGCAGCAGAGCCTGGCC 
AGCGCCGAAGGCATCGACTACATGACCTCGGTGAGCCGGCAGAACTATTCGACCATCTCCATCTACGCGCGG 
ATCGGCGCCAATACCGATCGCCTGGTCACCGAGCTGCTGGCCAAGTCCAACGAAGTGAAGAGCCAACTGCCG 
CCGGACGCCGAGGACCCGGTGCTGCAGAAGGAGGCCGCGGATGCCTCGGCGCTGATGTACATCAGCTTCTAC 
AGCGAGCAGATGAACAACCCGCAGATCACCGACTACCTGTCGCGGGTGATCCAGCCCAAGCTGGCGACCCTG 
CCCGGTATCGCCGAGGCGGAGATCCTCGGCAACCAGGTGTTCGCCATGCGCCTGTGGCTGGACCCGGTGAAG 
ATGGCCGCGTTCGGCGTCACCGCCGGCGAGATCAACCAGGCGGTGCAGCAGTACAACTTCCTCGCCGCCGCC 
GGCGAGGTGAAGGGCCAGTTGGTGGTCACCAGCGTCAATGCTTCCACCGACCTCAAGTCGCCCCAGGCCTTC 
GCCGCCATCCCGGTGAAGACCGACGGCGACCGCCGGGTGCTGATGGGCGATGTCGCACGGGTCGAACTGGGC 
GCCGCCAGCTACGACGCGATCAGTTCGTTCAATGGGATTCCCTCGGTCTACATCGGCATCAAGGGCACGCCC 
AGCGCCAACCCGCTGGACGTGATCAAGGAAGTGCGGGCGAAGATGCCCGAACTGGAAGAGCAATTGCCGCCC 
AACCTCAAGGTGTCCATCGCCTACGACGCCACGCGCTTCATCCAGGCCTCCATCGACGAAGTGGTGAAGACC 
CTCGGCGAGGCGGTGCTGATCGTCATCGTGGTGGTGTTCCTGTTCCTCGGCGCGTTCCGTTCGGTACTGATC 
CCGGTGGTGACCATTCCGCTGTCGATGATCGGCGTGTTGTTCTTCATGCAGGCCATGGGCTACTCGATCAAC 
CTGCTGACCCTGCTGGCGATGGTCCTGGCCATCGGCCTGGTGGTGGACGACGCGATCGTGGTGGTGGAAAAC 
ATCCACCGCCACATCGAGGAGGGCAAGCCGCCCTTCGAGGCCGCCCTGGAGGGCGCGCGGGAGATCGCCGTG 
CCGGTGGTCAGCATGACCATCACCCTCGCCGCGGTCTACGCGCCGATCGGCTTTCTCACCGGCCTCACCGGC 
GCCCTGTTCAAGGAATTCGCCTTCACCCTGGCCGGCGCGGTGATCATTTCCGGGATCGTCGCCCTGACCCTG 
TCGCCGATGATGTGCTCGCGCCTGTTGCGCCACGAGGAGAATCCCTCGGGCCTGGCGCATCGCCTCGACCTG 
ATCTTCGAGGGCCTGAAGCGACGCTACCAGCGCGCCCTCCACGGCACCCTGGACACCCGTCCGGTGGTCCTG 
GTGTTCGCCGTGCTGGTACTGGCGCTGATCCCGGTACTGCTGATGTTCACCAAGAAGGAGCTGGCGCCGGAA 
GAGGACCAGGGCATCGTGTTCCTCATGACCAACTCGCCGCAGACCGCCAACCTCGACTACCTGAATCGCTAT 
ACCGCCGAGTTCGAAGGCATCTTCCGCAGCTTCCCGGAGTATTACTCGGCGTTCCAGATCAACGGCTACAAC 
GGCGTCCAGGCCGGCATCGGCGGCATGCTGCTCAAGCCCTGGGACGAACGCGAAAAGAGCCAGATGGAATTG 
CTCCATGCGGTGCAGGCGAAGCTCAACGAGATTCCCGGCGTGCAGATCTTCGCCTTCAACCTGCCGTCGCTA 
CCGGGCACCGGCGAGGGCCTGCCATTCCAGTTCGTGCTCAACACCGCCAACGACTACGAGTCGCTGCTGCAA 
GTGGCGCAACGGGTGAAGCAGCGCGCCAGCGAGTCCGGCAAGTTCGCCTTCCTCGATCTCGACCTGGCGTTC 
GACAAACCCGAGCTGGTGGTCGACATCGACCGCGAGAAGGCCGCGCAGATGGGCGTCTCCATGCAGGACCTG 
GGGGTGGCGCTGGCCAGCCTGCTCGGCGAGGGCGAGATCAACCGCTTCACCATCGACGGGCGCAGCTACAAG 
GTCATCGCCCAGGTCGAACGCCCCTATCGCGACAATCCCGGCTGGCTCGGCAGCTACTACGTGAAGAGCCGC 
AACGGCCAACTGGTCGCCCTCTCGACCCTGATCGAGACCCACGAACGGGCACGGCCGCGGCAACTGAACCAG 
TTCCAGCAGCTCAACTCGGCGATCATCTCCGGCTTCCCCATCGTCAGCATGGGCGAGGCCATCGAGACGGTT 
CAGCAGATCGCCCGCGAGGAGGCCCCGCGCGGCTTCGCCGTCGACTACGCCGGCGCTTCCCGGCAATACGTG 
CAGGAAGGCAGCGCATTGCTGGTCACCTTCGGACTGGCCCTGGCGATCATCTTCCTGGTCCTCGCCGCACAG 
TTCGAAAGCTTCCGCGATCCGCTGGTGATCATGGTCACCGTGCCGCTGTCGATCTGCGGTGCGCTGATACCG 
CTGTTCCTCGGCGTGTCGAGCCTGAACATCTATACCCAGGTCGGCCTGGTGACACTGATCGGCCTGATCAGC 
AAACACGGCATCCTGATCGTCGAGTTCGCCAACCAGTTGCGCCACGAACAGGGGCTGGGGCGTCGCGAGGCC 
ATCGAGCAGGCCGCCGCGATCCGCCTGCGGCCGGTACTGATGACCACCGCGGCGATGGTGCTCGGGGTGATC 
CCACTGATCCTCGCCACCGGCGCCGGGGCGGTCAGTCGCTTCGACATCGGCATCGTGATCGCTACCGGCATG 
TCGGTGGGCACCCTCTTCACCCTGTTCGTCCTGCCGTGCATCTACACCCTGGTGGCCAGGCCGGATGCCCCG 
CCAgGCGTGACGCAAGCGGCAAACGCACACTGA