ViewGene
Locus Name:
GeneID:
Location:

GeneID:759
TrivialName: PA14_16250
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16250
PAO1 Ortholog Locus:PA3724
Sequence Length:1497
Protein Length:498
Start: 1387521
Stop: 1389017
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07111.1|AE004791_8 (AE004791) elastase LasB [Pseudomonas aeruginosa PAO1]
Identities = 493/498 (98%), Positives = 495/498 (99%)
Sequence:
ATGAAGAAGGTTTCTACGCTTGACCTGTTGTTCGTTGCGATCATGGGTGTTTCGCCGGCCGCTTTTGCCGCC
GACCTGATCGACGTGTCCAAACTCCCCAGCAAGGCTGCCCAGGGCGCGCCCGGCCCGGTCACCTTGCAAGCC
GCGGTCGGCGCCGGCGGTGCCGACGAACTGAAAGCGATCCGCAGCACGACCCTGCCCAACGGCAAGCAGGTC
ACCCGCTACGAGCAATTCCACAACGGCGTACGGGTGGTCGGCGAAGCCATCACCGAAGTCAAGGGTCCCGGC
AAGAGCGTGGCGGCGCGGCGCAGCGGCCATTTCGTCGCCAACATCGCCGCCGACCTGCCGGGCAGCACCACC
GCGGCGGTATCCGCCGAGCAGGTGCTGGCCCAGGCCAAGAGCCTGAAGGCCCAGGGCCGCAAGACCGAGAAT
GACAAAGTGGAACTGGTGATCCGCCTGGGCGAGAACAACATCGCCCAACTGGTCTACAACGTCTCCTACCTG
ATTCCCGGCGAGGGACTGTCGCGGCCGCATTTCGTCATCGACGCCAAGACCGGTGAAGTGCTCGATCAGTGG
GAAGGCCTGGCCCACGCCGAGGCGGGCGGCCCCGGTGGCAACCAGAAGATCGGCAAGTACACCTACGGTAGC
GACTACGGTCCGCTGATCGTCAACGACCGCTGCGAGATGGACGACGGCAACGTCATCACCGTCGACATGAAC
GGCAGCACCAACGACAGCAAGACCACGCCGTTCCGCTTCGCCTGCCCGACCAACACCTACAAGCAGGTCAAC
GGCGCTTATTCGCCACTGAACGACGCGCATTTCTTCGGCGGCGTGGTGTTCAACCTGTACCGGGACTGGTTC
GGCACCAGCCCGCTGACCCACAAGCTGTACATGAAGGTGCACTACGGGCGCAGCGTGGAGAACGCCTACTGG
GACGGCACGGCGATGCTCTTCGGCGACGGCGCCACCATGTTCTATCCGCTGGTGTCGCTGGACGTGGCGGCC
CACGAGGTCAGCCACGGCTTCACCGAGCAGAACTCCGGGCTGATCTACCGCGGGCAATCCGGCGGAATGAAC
GAGGCGTTCTCCGACATGGCCGGCGAGGCCGCCGAGTTCTACATGCGCGGCAAGAACGACTTCCTGATTGGC
TACGACATCAAGAAGGGCAGCGGTGCGTTGCGCTACATGGACCAGCCCAGCCGCGACGGGCGATCCATCGAC
AACGCCTCGCAGTACTACAACGGTATCGACGTGCACCACTCCAGCGGCGTGTACAACCGTGCGTTCTACCTG
CTGGCCAACTCGCCGGGCTGGGATACCCGCAAGGCCTTCGAGGTGTTCGTCGACGCCAACCGCTACTACTGG
ACCGCCACCAGCAACTACAACAGCGGTGCCTGTGGAGTGATTAGCTCGGCGCAGAACCGCAACTACTCGGCG
GCTGACGTCACCCGGGCGTTCAGCACCGTCGGCGTGACCTGCCCGAGCGCGTTGTAA
Translation:
MKKVSTLDLLFVAIMGVSPAAFAADLIDVSKLPSKAAQGAPGPVTLQAAVGAGGADELKAIRSTTLPNGKQV
TRYEQFHNGVRVVGEAITEVKGPGKSVAARRSGHFVANIAADLPGSTTAAVSAEQVLAQAKSLKAQGRKTEN
DKVELVIRLGENNIAQLVYNVSYLIPGEGLSRPHFVIDAKTGEVLDQWEGLAHAEAGGPGGNQKIGKYTYGS
DYGPLIVNDRCEMDDGNVITVDMNGSTNDSKTTPFRFACPTNTYKQVNGAYSPLNDAHFFGGVVFNLYRDWF
GTSPLTHKLYMKVHYGRSVENAYWDGTAMLFGDGATMFYPLVSLDVAAHEVSHGFTEQNSGLIYRGQSGGMN
EAFSDMAGEAAEFYMRGKNDFLIGYDIKKGSGALRYMDQPSRDGRSIDNASQYYNGIDVHHSSGVYNRAFYL
LANSPGWDTRKAFEVFVDANRYYWTATSNYNSGACGVISSAQNRNYSAADVTRAFSTVGVTCPSAL*
AnnotationID:768GeneID:759
AnnotatorUID: diggins
Modification Date/Time: 2005-04-22 08:08:06
Gene Name:lasB
Confidence Code:1
GeneProduct: elastase LasB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:metalloprotease
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3227
Secondary Functional Category(ies):
EC Number:3.4.24.26
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN78225.1| (AY161300) class 4 metalloprotease [Chromobacterium violaceum]
          Length = 489

 Score =  612 bits (1579), Expect = e-174
 Identities = 303/488 (62%), Positives = 367/488 (75%), Gaps = 12/488 (2%)

>gb|AAC72410.1| (AF102028) metalloprotease [Vibrio vulnificus]
          Length = 609

 Score =  478 bits (1231), Expect = e-134
 Identities = 251/501 (50%), Positives = 339/501 (67%), Gaps = 26/501 (5%)






 
Structural Features:
COG3227, LasB, Zinc metalloprotease (elastase) 
[Amino acid transport and metabolism]
CD-Length = 507 residues,  99.8% aligned
Score =  496 bits (1279), Expect = 2e-141
Identities = 184/521 (35%), Positives = 253/521 (48%), Gaps = 42/521 (8%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1521




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18663




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31831




  
Author/Investigator(s):
        





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type: 







    
Data:


    		




 
URL:

















    ReferenceID:31832




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:759_cdd.html


    		




 
URL:

















    ReferenceID:34252




  
Author/Investigator(s):
        Bever RA, Iglewski BH.





  
Title:
        Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene.




  
PubMed:
        2842313


MedLine:
        




    
Source:
J Bacteriol. 1988 Sep;170(9):4309-14.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2842313

















    ReferenceID:34253




  
Author/Investigator(s):
        Rust L, Pesci EC, Iglewski BH.





  
Title:
        Analysis of the Pseudomonas aeruginosa elastase (lasB) regulatory region.




  
PubMed:
        8576049


MedLine:
        




    
Source:
J Bacteriol. 1996 Feb;178(4):1134-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8576049









           
Homologs By Global Alignment 

           

       



     
Gene ID:759
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1338
                           PA3724_tr
                           translation of PA3724
                           498
                           98.99
                           99.39
                           0
                           2581.0
     

     
     
49712
                           gb|AAG07111.1|AE004791_8
                           (AE004791) elastase LasB [Pseudomonas aeruginosa PAO1]
                           498
                           98.99
                           99.39
                           0
                           2581.0
     

     
     
49713
                           dbj|BAB79621.1|
                           (AB029328) PST-01 protease (pseudolysin) precursor [Pseudomonas aeruginosa]
                           498
                           98.99
                           99.39
                           0
                           2581.0
     

     
     
49714
                           gb|AAB36615.1|
                           (M19472) elastase precursor [Pseudomonas aeruginosa]
                           498
                           98.99
                           99.39
                           0
                           2581.0
     

     
     
49715
                           gb|AAA25811.1|
                           (M24531) elastase precursor (EC 3.4.24.4) [Pseudomonas aeruginosa]
                           498
                           98.79
                           99.39
                           0
                           2577.0
     

     






      

          
GC ORFID: 43761How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3724Glimmer Score: 

          
Start: 3566416Stop: 3564920

          
Length: 1497


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3724 translation of PA3724Homolog Bit Score 998.0

          
Other Homologs:  t_PA3724 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGAAGGTTTCTACGCTTGACCTGTTGTTCGTTGCGATCATGGGTGTTTCGCCGGCCGCTTTTGCCGCC 
GACCTGATCGACGTGTCCAAACTCCCCAGCAAGGCTGCCCAGGGCGCGCCCGGCCCGGTCACCTTGCAAGCC 
GCGGTCGGCGCCGGCGGTGCCGACGAACTGAAAGCGATCCGCAGCACGACCCTGCCCAACGGCAAGCAGGTC 
ACCCGCTACGAGCAATTCCACAACGGCGTACGGGTGGTCGGCGAAGCCATCACCGAAGTCAAGGGTCCCGGC 
AAGAGCGTGGCGGCGCGGCGCAGCGGCCATTTCGTCGCCAACATCGCCGCCGACCTGCCGGGCAGCACCACC 
GCGGCGGTATCCGCCGAGCAGGTGCTGGCCCAGGCCAAGAGCCTGAAGGCCCAGGGCCGCAAGACCGAGAAT 
GACAAAGTGGAACTGGTGATCCGCCTGGGCGAGAACAACATCGCCCAACTGGTCTACAACGTCTCCTACCTG 
ATTCCCGGCGAGGGACTGTCGCGGCCGCATTTCGTCATCGACGCCAAGACCGGTGAAGTGCTCGATCAGTGG 
GAAGGCCTGGCCCACGCCGAGGCGGGCGGCCCCGGTGGCAACCAGAAGATCGGCAAGTACACCTACGGTAGC 
GACTACGGTCCGCTGATCGTCAACGACCGCTGCGAGATGGACGACGGCAACGTCATCACCGTCGACATGAAC 
GGCAGCACCAACGACAGCAAGACCACGCCGTTCCGCTTCGCCTGCCCGACCAACACCTACAAGCAGGTCAAC 
GGCGCTTATTCGCCACTGAACGACGCGCATTTCTTCGGCGGCGTGGTGTTCAACCTGTACCGGGACTGGTTC 
GGCACCAGCCCGCTGACCCACAAGCTGTACATGAAGGTGCACTACGGGCGCAGCGTGGAGAACGCCTACTGG 
GACGGCACGGCGATGCTCTTCGGCGACGGCGCCACCATGTTCTATCCGCTGGTGTCGCTGGACGTGGCGGCC 
CACGAGGTCAGCCACGGCTTCACCGAGCAGAACTCCGGGCTGATCTACCGCGGGCAATCCGGCGGAATGAAC 
GAGGCGTTCTCCGACATGGCCGGCGAGGCCGCCGAGTTCTACATGCGCGGCAAGAACGACTTCCTGATTGGC 
TACGACATCAAGAAGGGCAGCGGTGCGTTGCGCTACATGGACCAGCCCAGCCGCGACGGGCGATCCATCGAC 
AACGCCTCGCAGTACTACAACGGTATCGACGTGCACCACTCCAGCGGCGTGTACAACCGTGCGTTCTACCTG 
CTGGCCAACTCGCCGGGCTGGGATACCCGCAAGGCCTTCGAGGTGTTCGTCGACGCCAACCGCTACTACTGG 
ACCGCCACCAGCAACTACAACAGCGGTGCCTGTGGAGTGATTAGCTCGGCGCAGAACCGCAACTACTCGGCG 
GCTGACGTCACCCGGGCGTTCAGCACCGTCGGCGTGACCTGCCCGAGCGCGTTGTAA