ViewGene
Locus Name:
GeneID:
Location:

GeneID:757
TrivialName: PA14_68140
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_68140
PAO1 Ortholog Locus:PA5160
Sequence Length:1530
Protein Length:509
Start: 6080538
Stop: 6082067
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08545.1|AE004928_11 (AE004928) drug efflux transporter [Pseudomonas aeruginosa PAO1]
Identities = 505/509 (99%), Positives = 508/509 (99%)
Sequence:
ATGAGCGAAAGCAGCAGCTTCTCCCCGCCCAGCCTGGTGATGGCGACCATCGGCCTGTCCCTGGCGACTTTC
ATGCAGGTGCTCGATACCACCATCGCCAACGTCGCCCTGCCGACCATCTCCGGTAACCTCGGGGTGAGTTCG
GAACAGGGCACCTGGGTGATCACCTCGTTCGCCGTGAGCAACGCCATCGCCCTGCCGCTGACCGGCTGGCTG
GCCCGGCGGGTCGGCGAGGTGCGCCTGTTCATCGCCGCCGCGCTGCTGTTCGTACTCGCCTCGTTCCTCTGC
GGCATCGCCCAGTCGATGCCTTCTCTGGTCGGCTTCCGCGCCTTGCAGGGCTTCGTCGCCGGGCCGCTGTAT
CCGATCACCCAGACCCTGCTGATCTCCATCTACCCACCGGCGAAGCGCGGCATGGCCCTGGCCCTGCTGGCG
ATGGTCACGGTGGTGGCGCCGATCGCCGGGCCGATCCTCGGCGGCTGGATCACCGACGACTACAGCTGGCCG
TGGATCTTCTTCATCAACGTGCCGGTCGGCCTGTTCGCCGCTTTCGTCGTCTACCAGCAACTGAAGGCACGC
CCGGTGGTGATCAAGAAGGCGCCGATGGACTACGTCGGCCTGATCGCCCTGGTGATCGGCGTCGGCGCCCTG
CAGATCGTCCTCGACAAGGGCAACGACCTGGACTGGTTCGAGTCGAACTTCATCGTCGGCGGCGCGCTGATC
GCGGCGATCGCCCTGGCCTTCTTCATCATCTGGGAATTCACCGACCGCCATCCGATCGTCAACCTGCGCCTG
TTCGCCCACCGCAACTTCGCCGCCGGCACCCTGGCGCTGGTGCTGGGCTATGCCGCATTCTTCGGCATCAAC
CTGCTGCTCCCGCAATGGCTACAGACCCAGATGGGCTACACCGCCACCTGGGCCGGGCTGGCCGCCGCGCCA
ATCGGCATCCTCCCGGTGTTCCTCTCGCCGCTGGTCGGCCGCTACGCCAACCATTTCGACCTGCGCATACTC
GCCGGCCTGTCGTTCCTGGCGATGGCCATCACCTGCTTCATGCGCGCCAACTTCACCACCGAGGTCGACTAC
CAGCACATCGCCATCGTGCAGTTGATCATGGGGCTCGGCGTGGCGTTCTTCTTCATGCCGATCCTGAGCATC
CTGCTCTCCGACCTGCCCCCCGACCAGATCGCCGACGGCTCCGGACTGGCCACCTTCCTGCGCACCCTCGGC
GGCAGCTTCGCCGCCTCGCTGACCACCTGGATCTGGAACCGCCGCGCCACCCAGCACCACGCCTACCTCAGC
GAAAACATCAGCCTCTACGACCCGGCCACCCACGAAACCCTCGCCCAGCTCGGCGGCAACACCCAGGCGAAC
GCGGCGCTGCTCGACCGGATGGTGCAGAGCCAGGCCTACATGATGTCCACCATCGACTACTTCACCATGCTC
GGCTGGCTGTTCCTCGCCCTGCTGGTGATCATCTGGCTGGCCCGCCCGCCGTTCGGCGCCAAGCCCGGCGCG
GCCGCCTCCGGGCACTGA
Translation:
MSESSSFSPPSLVMATIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAVSNAIALPLTGWL
ARRVGEVRLFIAAALLFVLASFLCGIAQSMPSLVGFRALQGFVAGPLYPITQTLLISIYPPAKRGMALALLA
MVTVVAPIAGPILGGWITDDYSWPWIFFINVPVGLFAAFVVYQQLKARPVVIKKAPMDYVGLIALVIGVGAL
QIVLDKGNDLDWFESNFIVGGALIAAIALAFFIIWEFTDRHPIVNLRLFAHRNFAAGTLALVLGYAAFFGIN
LLLPQWLQTQMGYTATWAGLAAAPIGILPVFLSPLVGRYANHFDLRILAGLSFLAMAITCFMRANFTTEVDY
QHIAIVQLIMGLGVAFFFMPILSILLSDLPPDQIADGSGLATFLRTLGGSFAASLTTWIWNRRATQHHAYLS
ENISLYDPATHETLAQLGGNTQANAALLDRMVQSQAYMMSTIDYFTMLGWLFLALLVIIWLARPPFGAKPGA
AASGH*
AnnotationID:766GeneID:757
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 18:06:00
Gene Name:
Confidence Code:2
GeneProduct:drug efflux transporter
Cell Localization:()
Synonyms:PA5160, pmrB, emrB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:multidrug resistant protein
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69149.1|AE016548_8 (AE016787) drug resistance transporter, EmrB/QacA family
           [Pseudomonas putida KT2440]
          Length = 511

 Score =  808 bits (2088), Expect = 0.0
 Identities = 386/506 (76%), Positives = 446/506 (88%)

>gb|AAM36317.1| (AE011776) multidrug resistance membrane translocase [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 526

 Score =  665 bits (1715), Expect = 0.0
 Identities = 327/509 (64%), Positives = 403/509 (79%), Gaps = 3/509 (0%)

>gb|AAM84503.1|AE013695_5 (AE013695) putative membrane translocase [Yersinia pestis KIM]
          Length = 511

 Score =  516 bits (1330), Expect = e-145
 Identities = 257/500 (51%), Positives = 350/500 (70%), Gaps = 4/500 (0%)








 
Structural Features:






   
Genomic Context:






   
Comment:


















    ReferenceID:1517




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18659




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34913




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:757_cdd.html


    		




 
URL:

















    ReferenceID:35776




  
Author/Investigator(s):
        Lomovskaya O, Lewis K.





  
Title:
        Emr, an Escherichia coli locus for multidrug resistance.




  
PubMed:
        1409590


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):8938-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1409590









           
Homologs By Global Alignment 

           

       



     
Gene ID:757
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1331
                           PA5160_tr
                           translation of PA5160
                           509
                           99.21
                           99.80
                           0
                           2584.0
     

     
     
49633
                           gb|AAG08545.1|AE004928_11
                           (AE004928) drug efflux transporter [Pseudomonas aeruginosa PAO1]
                           509
                           99.21
                           99.80
                           0
                           2584.0
     

     
     
49634
                           gb|AAC31388.1|
                           (AF047693) multidrug resistance efflux pump homolog PmrB [Pseudomonas aeruginosa]
                           509
                           99.21
                           99.80
                           0
                           2584.0
     

     
     
49635
                           gb|AAN69149.1|AE016548_8
                           (AE016787) drug resistance transporter, EmrB/QacA family [Pseudomonas putida KT2440]
                           511
                           75.92
                           87.86
                           2
                           2087.0
     

     
     
49636
                           gb|AAO57216.1|
                           (AE016869) drug resistance transporter, EmrB/QacA family [Pseudomonas syringae pv. tomato str. DC3000]
                           512
                           75.39
                           87.50
                           3
                           2078.0
     

     






      

          
GC ORFID: 43759How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5160Glimmer Score: 

          
Start: 5344224Stop: 5345753

          
Length: 1530


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5160 translation of PA5160Homolog Bit Score 999.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene759 (ORF: BLASTX 804.0), t_PA5160 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGAAAGCAGCAGCTTCTCCCCGCCCAGCCTGGTGATGGCGACCATCGGCCTGTCCCTGGCGACTTTC 
ATGCAGGTGCTCGATACCACCATCGCCAACGTCGCCCTGCCGACCATCTCCGGTAACCTCGGGGTGAGTTCG 
GAACAGGGCACCTGGGTGATCACCTCGTTCGCCGTGAGCAACGCCATCGCCCTGCCGCTGACCGGCTGGCTG 
GCCCGGCGGGTCGGCGAGGTGCGCCTGTTCATCGCCGCCGCGCTGCTGTTCGTACTCGCCTCGTTCCTCTGC 
GGCATCGCCCAGTCGATGCCTTCTCTGGTCGGCTTCCGCGCCTTGCAGGGCTTCGTCGCCGGGCCGCTGTAT 
CCGATCACCCAGACCCTGCTGATCTCCATCTACCCACCGGCGAAGCGCGGCATGGCCCTGGCCCTGCTGGCG 
ATGGTCACGGTGGTGGCGCCGATCGCCGGGCCGATCCTCGGCGGCTGGATCACCGACGACTACAGCTGGCCG 
TGGATCTTCTTCATCAACGTGCCGGTCGGCCTGTTCGCCGCTTTCGTCGTCTACCAGCAACTGAAGGCACGC 
CCGGTGGTGATCAAGAAGGCGCCGATGGACTACGTCGGCCTGATCGCCCTGGTGATCGGCGTCGGCGCCCTG 
CAGATCGTCCTCGACAAGGGCAACGACCTGGACTGGTTCGAGTCGAACTTCATCGTCGGCGGCGCGCTGATC 
GCGGCGATCGCCCTGGCCTTCTTCATCATCTGGGAATTCACCGACCGCCATCCGATCGTCAACCTGCGCCTG 
TTCGCCCACCGCAACTTCGCCGCCGGCACCCTGGCGCTGGTGCTGGGCTATGCCGCATTCTTCGGCATCAAC 
CTGCTGCTCCCGCAATGGCTACAGACCCAGATGGGCTACACCGCCACCTGGGCCGGGCTGGCCGCCGCGCCA 
ATCGGCATCCTCCCGGTGTTCCTCTCGCCGCTGGTCGGCCGCTACGCCAACCATTTCGACCTGCGCATACTC 
GCCGGCCTGTCGTTCCTGGCGATGGCCATCACCTGCTTCATGCGCGCCAACTTCACCACCGAGGTCGACTAC 
CAGCACATCGCCATCGTGCAGTTGATCATGGGGCTCGGCGTGGCGTTCTTCTTCATGCCGATCCTGAGCATC 
CTGCTCTCCGACCTGCCCCCCGACCAGATCGCCGACGGCTCCGGACTGGCCACCTTCCTGCGCACCCTCGGC 
GGCAGCTTCGCCGCCTCGCTGACCACCTGGATCTGGAACCGCCGCGCCACCCAGCACCACGCCTACCTCAGC 
GAAAACATCAGCCTCTACGACCCGGCCACCCACGAAACCCTCGCCCAGCTCGGCGGCAACACCCAGGCGAAC 
GCGGCGCTGCTCGACCGGATGGTGCAGAGCCAGGCCTACATGATGTCCACCATCGACTACTTCACCATGCTC 
GGCTGGCTGTTCCTCGCCCTGCTGGTGATCATCTGGCTGGCCCGCCCGCCGTTCGGCGCCAAGCCCGGCGCG 
GCCGCCTCCGGGCACTGA