ViewGene
Locus Name:
GeneID:
Location:

GeneID:752
TrivialName: PA14_04320
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04320
PAO1 Ortholog Locus:PA0331
Sequence Length:1515
Protein Length:504
Start: 386260
Stop: 387774
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03720.1|AE004471_3 (AE004471) threonine dehydratase, biosynthetic [Pseudomonas aeruginosa PAO1]
Identities = 503/504 (99%), Positives = 504/504 (100%)
Sequence:
ATGCTCGAACAATACGTCAAGAAGATCCTCACCTCGCGCGTCTATGACGTCGCGGTGGAAACGCCCCTGCAA
CCCGCCCGCCAGTTGTCCGAACGCCTCGGCAACCAGGTCCTGCTCAAGCGCGAGGACCTGCAGCCGGTGTTT
TCCTTCAAGATCCGCGGCGCCTACAACAAGGTCGCGCAGCTCACCGAGGAGGAGAAGGCCCGCGGGGTGATC
GCCGCCTCGGCCGGCAACCACGCCCAGGGCCTGGCCCTGGCGGCCAAGCGGCAGGGGATCAGGGCGGTCATC
GTGATGCCCAAGACCACCCCCGAGATCAAGGTCCAGGCGGTGCGCGCCCACGGCGCCAAGGCGGTGCTGCAC
GGCGACGCCTTCCCCGAGGCGCTGGCCCACGCGCTGAAGCTGGTCGACGAGAAGGGCTACACCTTCGTCCAT
CCCTACGACGATCCGGACACCATCGCCGGCCAGGGCACCGTGGCCATGGAGATCCTCCGCCAGCAGCCCGGC
CGCCTCGATGCGATCTTCGTCCCGGTGGGCGGTGGCGGATTGGTCGCCGGCATCGCCGCCTACGTCAAGTAC
CTGCGTCCGGAGATCAAGGTGATCGGCGTCGAGCCGGACGAGTCGAACTGCCTGCAGGCGGCCATGGCCGCC
GGCGAGCGGGTGATGCTGGGCCAGGTCGGGCTGTTCGCCGACGGCGTGGCGGTGGCGCAGATCGGCCAGCAC
ACCTTCGACATCTGCAAGGACCACGTCGACGAAGTGATCACCGTCAGCACCGACGAGATCTGTGCGGCGATC
AAGGACATCTACGACGACACCCGCTCGATCACCGAGCCGGCCGGCGCGCTGGCGGTGGCCGGGATCAAGAAG
TACGTTGAGCGCGAGCGCGCCGAGGGGCAGACCCTGGTGGCGATCGACTCCGGCGCCAACGTCAACTTCGAT
CGCCTGCGGCATGTCGCCGAACGCGCCGAACTGGGCGAGCGCCGCGAAGCGATCATCGCGGTGACCATTCCC
GAGCGGCCGGGCAGCTTCAAGGCCTTCTGCGAGGCGGTCGGCAAGCGCCAGATCACCGAGTTCAACTATCGC
TACCACAGCGGCAGCGAGGCGCACATCTTCGTCGGCGTGCAGACCCACCCGGAGAACGACCCGCGCGAGGCG
CTGGTGGCCTATCTGCGCGAGAAGGGCTTCCCGGTCCTCGACCTGACCGACAACGAACTGGCCAAGCTGCAC
ATCCGCCACATGGTTGGCGGCCATGCAGTGAAAGTCAGCGACGAGATGGTGTTCCGCTTCGAATTCCCCGAG
CGGCCCGGCGCGCTGTTCAACTTCCTCACCAAGCTCGGCGGGCGCTGGAACATCTCGATGTTCCACTACCGC
AACCATGGCGCGGCCGACGGCCGGGTGGTGGCCGGGTTGCAGGTGCCGGAAGACGAGCGCCACCTGATCCCG
CAGACCCTGGAGGCGATCGGCTATCCGTACTGGGACGAGACCGCCAACCCGGCCTACCAGCTGTTCCTCGGC
TGA
Translation:
MLEQYVKKILTSRVYDVAVETPLQPARQLSERLGNQVLLKREDLQPVFSFKIRGAYNKVAQLTEEEKARGVI
AASAGNHAQGLALAAKRQGIRAVIVMPKTTPEIKVQAVRAHGAKAVLHGDAFPEALAHALKLVDEKGYTFVH
PYDDPDTIAGQGTVAMEILRQQPGRLDAIFVPVGGGGLVAGIAAYVKYLRPEIKVIGVEPDESNCLQAAMAA
GERVMLGQVGLFADGVAVAQIGQHTFDICKDHVDEVITVSTDEICAAIKDIYDDTRSITEPAGALAVAGIKK
YVERERAEGQTLVAIDSGANVNFDRLRHVAERAELGERREAIIAVTIPERPGSFKAFCEAVGKRQITEFNYR
YHSGSEAHIFVGVQTHPENDPREALVAYLREKGFPVLDLTDNELAKLHIRHMVGGHAVKVSDEMVFRFEFPE
RPGALFNFLTKLGGRWNISMFHYRNHGAADGRVVAGLQVPEDERHLIPQTLEAIGYPYWDETANPAYQLFLG
*
AnnotationID:761GeneID:752
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 14:02:40
Gene Name:ilvA1
Confidence Code:2
GeneProduct:threonine dehydratase, biosynthetic
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1171,pfam00291
Secondary Functional Category(ies):
EC Number:4.2.1.16
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70714.1|AE016715_3 (AE016793) threonine dehydratase, biosynthetic [Pseudomonas putida
           KT2440]
          Length = 504

 Score =  882 bits (2280), Expect = 0.0
 Identities = 436/504 (86%), Positives = 463/504 (91%)

>emb|CAE31766.1| (BX640440) threonine dehydratase biosynthetic [Bordetella
           bronchiseptica]
          Length = 526

 Score =  777 bits (2006), Expect = 0.0
 Identities = 379/503 (75%), Positives = 439/503 (87%)








 
Structural Features:
COG1171, IlvA, Threonine dehydratase [Amino acid transport and metabolism] 
CD-Length = 347 residues,  99.1% aligned
Score =  438 bits (1129), Expect = 6e-124
Identities = 193/345 (55%), Positives = 254/345 (73%), Gaps = 5/345 (1%)

pfam00291, PALP, Pyridoxal-phosphate dependent enzyme. 
CD-Length = 298 residues, 100.0% aligned
Score =  241 bits (617), Expect = 1e-64
Identities = 112/303 (36%), Positives = 163/303 (53%), Gaps = 14/303 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1507




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18649




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33676




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:752_cdd.html


    		




 
URL:

















    ReferenceID:35423




  
Author/Investigator(s):
        Cox JL, Cox BJ, Fidanza V, Calhoun DH.





  
Title:
        The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12.




  
PubMed:
        3315862


MedLine:
        




    
Source:
Gene. 1987;56(2-3):185-98.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3315862









           
Homologs By Global Alignment 

           

       



     
Gene ID:752
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1324
                           PA0331_tr
                           translation of PA0331
                           504
                           99.80
                           99.99
                           0
                           2586.0
     

     
     
49541
                           gb|AAG03720.1|AE004471_3
                           (AE004471) threonine dehydratase, biosynthetic [Pseudomonas aeruginosa PAO1]
                           504
                           99.80
                           99.99
                           0
                           2586.0
     

     
     
49542
                           gb|AAN70714.1|AE016715_3
                           (AE016793) threonine dehydratase, biosynthetic [Pseudomonas putida KT2440]
                           504
                           86.50
                           91.86
                           0
                           2280.0
     

     
     
49544
                           gb|AAO57275.1|
                           (AE016869) threonine dehydratase, biosynthetic [Pseudomonas syringae pv. tomato str. DC3000]
                           507
                           83.43
                           90.92
                           3
                           2216.0
     

     
     
49543
                           gb|AAO58714.1|
                           (AE016875) threonine dehydratase, biosynthetic [Pseudomonas syringae pv. tomato str. DC3000]
                           514
                           83.26
                           90.27
                           10
                           2236.0
     

     






      

          
GC ORFID: 43754How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0331Glimmer Score: 

          
Start: 6187602Stop: 6189116

          
Length: 1515


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0331 translation of PA0331Homolog Bit Score 1000.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6529 (ORF: BLASTX 865.0), t_PA1326 (ORF: BLASTX 813.0), t_PA0331 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCGAACAATACGTCAAGAAGATCCTCACCTCGCGCGTCTATGACGTCGCGGTGGAAACGCCCCTGCAA 
CCCGCCCGCCAGTTGTCCGAACGCCTCGGCAACCAGGTCCTGCTCAAGCGCGAGGACCTGCAGCCGGTGTTT 
TCCTTCAAGATCCGCGGCGCCTACAACAAGGTCGCGCAGCTCACCGAGGAGGAGAAGGCCCGCGGGGTGATC 
GCCGCCTCGGCCGGCAACCACGCCCAGGGCCTGGCCCTGGCGGCCAAGCGGCAGGGGATCAGGGCGGTCATC 
GTGATGCCCAAGACCACCCCCGAGATCAAGGTCCAGGCGGTGCGCGCCCACGGCGCCAAGGCGGTGCTGCAC 
GGCGACGCCTTCCCCGAGGCGCTGGCCCACGCGCTGAAGCTGGTCGACGAGAAGGGCTACACCTTCGTCCAT 
CCCTACGACGATCCGGACACCATCGCCGGCCAGGGCACCGTGGCCATGGAGATCCTCCGCCAGCAGCCCGGC 
CGCCTCGATGCGATCTTCGTCCCGGTGGGCGGTGGCGGATTGGTCGCCGGCATCGCCGCCTACGTCAAGTAC 
CTGCGTCCGGAGATCAAGGTGATCGGCGTCGAGCCGGACGAGTCGAACTGCCTGCAGGCGGCCATGGCCGCC 
GGCGAGCGGGTGATGCTGGGCCAGGTCGGGCTGTTCGCCGACGGCGTGGCGGTGGCGCAGATCGGCCAGCAC 
ACCTTCGACATCTGCAAGGACCACGTCGACGAAGTGATCACCGTCAGCACCGACGAGATCTGTGCGGCGATC 
AAGGACATCTACGACGACACCCGCTCGATCACCGAGCCGGCCGGCGCGCTGGCGGTGGCCGGGATCAAGAAG 
TACGTTGAGCGCGAGCGCGCCGAGGGGCAGACCCTGGTGGCGATCGACTCCGGCGCCAACGTCAACTTCGAT 
CGCCTGCGGCATGTCGCCGAACGCGCCGAACTGGGCGAGCGCCGCGAAGCGATCATCGCGGTGACCATTCCC 
GAGCGGCCGGGCAGCTTCAAGGCCTTCTGCGAGGCGGTCGGCAAGCGCCAGATCACCGAGTTCAACTATCGC 
TACCACAGCGGCAGCGAGGCGCACATCTTCGTCGGCGTGCAGACCCACCCGGAGAACGACCCGCGCGAGGCG 
CTGGTGGCCTATCTGCGCGAGAAGGGCTTCCCGGTCCTCGACCTGACCGACAACGAACTGGCCAAGCTGCAC 
ATCCGCCACATGGTTGGCGGCCATGCAGTGAAAGTCAGCGACGAGATGGTGTTCCGCTTCGAATTCCCCGAG 
CGGCCCGGCGCGCTGTTCAACTTCCTCACCAAGCTCGGCGGGCGCTGGAACATCTCGATGTTCCACTACCGC 
AACCATGGCGCGGCCGACGGCCGGGTGGTGGCCGGGTTGCAGGTGCCGGAAGACGAGCGCCACCTGATCCCG 
CAGACCCTGGAGGCGATCGGCTATCCGTACTGGGACGAGACCGCCAACCCGGCCTACCAGCTGTTCCTCGGC 
TGA