ViewGene
Locus Name:
GeneID:
Location:

GeneID:751
TrivialName: PA14_23920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23920
PAO1 Ortholog Locus:PA3108
Sequence Length:1506
Protein Length:501
Start: 2078376
Stop: 2079881
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06496.1|AE004735_3 (AE004735) amidophosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 501/501 (100%), Positives = 501/501 (100%)
Sequence:
ATGTGTGGCATCGTCGGTATCGTGGGCAAGTCGAACGTCAATCAGGCGCTGTATGACGCGCTCACCGTCCTC
CAACATCGCGGCCAGGACGCTGCCGGTATCGTCACCTGCCATGACGATAAGCTGTACCTGCGCAAGGACAAC
GGCCTGGTCCGCGACGTCTTCCAGCAGCGCCACATGCAGCGCCTGATCGGCAGCGTCGGCATCGGCCATGTG
CGTTACCCGACCGCCGGCAGCTCCAGCTCGGCGGAGGCGCAGCCGTTCTACGTCAACTCGCCCTACGGCATT
ACCCTGGCGCACAACGGCAACCTGACCAATGTCGAGCAGTTGGCCAAGGAAATCTACGAATCCGACCTGCGC
CACGTGAACACCAACTCCGATTCGGAAGTGCTGCTCAACGTGTTCGCCCACGAGCTGGCGGTGCGCAACAAG
CTGCAGCCCACCGAGGAGGACATCTTCGCCGCGGTTTCCTGCGTGCACGACCGTTGCGTCGGCGGCTACGCG
GTGGTGGCGATGATCACCGGCCATGGCATCGTCGGTTTCCGCGACCCCAATGCGATCCGTCCGATCGTCTTC
GGCCAGCGGCATACCGAGAACGGCGTCGAATACATGATCGCCTCCGAGAGCGTGGCCCTGGACGTACTCGGC
TTCACCCTGATCCGCGACCTGGCGCCGGGCGAGGCGGTGTACATCACCGAGGAAGGCAAGCTCTATACCCGC
CAGTGCGCGAAGGCGCCGAAGTACGCGCCGTGCATCTTCGAGCACGTCTACCTGGCGCGTCCGGATTCGATC
ATGGACGGCATCTCGGTGTACAAGGCGCGCCTGCGCATGGGCGAGAAGCTTGCCGACAAGATCCTCCGCGAG
CGTCCCGACCACGACATCGACGTGGTCATCCCGATCCCCGACACCAGCCGCACCGCGGCGCTGGAACTGGCC
AACCGGCTCGGCGTGAAGTTCCGCGAGGGCTTCGTCAAGAACCGCTACATCGGCCGCACCTTCATCATGCCC
GGCCAGGCGGCGCGGAAGAAGTCCGTGCGGCAGAAGCTCAACGCCATCGAGCTGGAGTTCCGCGGCAAGAAC
GTGATGCTGGTGGATGACTCCATCGTCCGCGGCACCACCTGCAAGCAGATCATCCAGATGGCCCGCGAGGCC
GGGGCGAAGAACGTCTACTTCTGCTCCGCCGCGCCGGCCGTGCGCTACCCCAACGTCTACGGCATCGACATG
CCGAGTGCGCACGAGCTGATCGCGCACAATCGCAGCACCGAGGACGTCAGCAAGCTGATCGGCGCCGACTGG
CTGGTCTACCAGGACCTGCCCGACCTGATCGACGCGGTCGGCGGCGGCAAGATCAAGATCGATCACTTCGAC
TGCGCCGTGTTCGACGGCGAGTACGTGACCGGCGACGTCAACGAGGCCTACCTGAACCGGATCGAACAGGCG
CGCAACGATGCGACCAAGGCCAAGAGCCAGGCGGTCAGCGCGATCATCGATCTGTACAACGACTGA
Translation:
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTCHDDKLYLRKDNGLVRDVFQQRHMQRLIGSVGIGHV
RYPTAGSSSSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLRHVNTNSDSEVLLNVFAHELAVRNK
LQPTEEDIFAAVSCVHDRCVGGYAVVAMITGHGIVGFRDPNAIRPIVFGQRHTENGVEYMIASESVALDVLG
FTLIRDLAPGEAVYITEEGKLYTRQCAKAPKYAPCIFEHVYLARPDSIMDGISVYKARLRMGEKLADKILRE
RPDHDIDVVIPIPDTSRTAALELANRLGVKFREGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKN
VMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRYPNVYGIDMPSAHELIAHNRSTEDVSKLIGADW
LVYQDLPDLIDAVGGGKIKIDHFDCAVFDGEYVTGDVNEAYLNRIEQARNDATKAKSQAVSAIIDLYND*
AnnotationID:760GeneID:751
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 06:06:30
Gene Name:purF
Confidence Code:2
GeneProduct: amidophosphoribosyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:glutamine phosphoribosylpyrophosphate amidotransferase
Functional Category Confidence Code:5
COGs:COG0034
Secondary Functional Category(ies):nucleotide biosynthesis and metabolism
EC Number:2.4.2.14
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67614.1|AE016391_5 (AE016781) amidophosphoribosyltransferase [Pseudomonas putida
           KT2440]
          Length = 501

 Score =  927 bits (2395), Expect = 0.0
 Identities = 456/501 (91%), Positives = 485/501 (96%)

>gb|AAO90426.1| (AE016962) amidophosphoribosyltransferase [Coxiella burnetii RSA
           493]
          Length = 506

 Score =  641 bits (1654), Expect = 0.0
 Identities = 312/497 (62%), Positives = 390/497 (78%), Gaps = 4/497 (0%)

>gb|AAO10395.1|AE016803_182 (AE016803) Glutamine phosphoribosylpyrophosphate amidotransferase
           [Vibrio vulnificus CMCP6]
          Length = 504

 Score =  628 bits (1620), Expect = e-179
 Identities = 313/503 (62%), Positives = 390/503 (77%), Gaps = 3/503 (0%)








 
Structural Features:
COG0034, PurF, Glutamine phosphoribosylpyrophosphate amidotransferase 
[Nucleotide transport and metabolism]
CD-Length = 470 residues,  99.4% aligned
Score =  623 bits (1609), Expect = 1e-179
Identities = 259/488 (53%), Positives = 324/488 (66%), Gaps = 26/488 (5%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1505




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18647




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32416




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:751_cdd.html


    		




 
URL:

















    ReferenceID:33906




  
Author/Investigator(s):
        Rolfes RJ, Zalkin H.





  
Title:
        Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene.




  
PubMed:
        3058703


MedLine:
        




    
Source:
J Biol Chem. 1988 Dec 25;263(36):19649-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3058703

















    ReferenceID:33907




  
Author/Investigator(s):
        Sampei G, Mizobuchi K.





  
Title:
        Nucleotide sequence of the Escherichia coli purF gene encoding amidophosphoribosyltransferase for de novo purine nucleotide synthesis.




  
PubMed:
        3047685


MedLine:
        




    
Source:
Nucleic Acids Res. 1988 Sep 12;16(17):8717.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3047685

















    ReferenceID:33908




  
Author/Investigator(s):
        Makaroff CA, Zalkin H.





  
Title:
        Regulation of Escherichia coli purF. Analysis of the control region of a pur regulon gene.




  
PubMed:
        2991286


MedLine:
        




    
Source:
J Biol Chem. 1985 Aug 25;260(18):10378-87.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2991286

















    ReferenceID:33909




  
Author/Investigator(s):
        Tso JY, Zalkin H, van Cleemput M, Yanofsky C, Smith JM.





  
Title:
        Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase.




  
PubMed:
        6277938


MedLine:
        




    
Source:
J Biol Chem. 1982 Apr 10;257(7):3525-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6277938









           
Homologs By Global Alignment 

           

       



     
Gene ID:751
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1323
                           PA3108_tr
                           translation of PA3108
                           501
                           99.99
                           99.99
                           0
                           2589.0
     

     
     
49476
                           gb|AAG06496.1|AE004735_3
                           (AE004735) amidophosphoribosyltransferase [Pseudomonas aeruginosa PAO1]
                           501
                           99.99
                           99.99
                           0
                           2589.0
     

     
     
49478
                           gb|AAO57280.1|
                           (AE016869) amidophosphoribosyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           502
                           91.23
                           96.01
                           1
                           2366.0
     

     
     
49477
                           gb|AAN67614.1|AE016391_5
                           (AE016781) amidophosphoribosyltransferase [Pseudomonas putida KT2440]
                           501
                           91.01
                           96.80
                           0
                           2395.0
     

     
     
49482
                           emb|CAC93011.1|
                           (AJ414153) amidophosphoribosyltransferase [Yersinia pestis CO92]
                           507
                           64.49
                           78.30
                           8
                           1641.0
     

     






      

          
GC ORFID: 43753How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3108Glimmer Score: 

          
Start: 2875555Stop: 2874050

          
Length: 1506


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3108 translation of PA3108Homolog Bit Score 1001.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7134 (ORF: BLASTX 914.0), t_PA3108 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTGTGGCATCGTCGGTATCGTGGGCAAGTCGAACGTCAATCAGGCGCTGTATGACGCGCTCACCGTCCTC 
CAACATCGCGGCCAGGACGCTGCCGGTATCGTCACCTGCCATGACGATAAGCTGTACCTGCGCAAGGACAAC 
GGCCTGGTCCGCGACGTCTTCCAGCAGCGCCACATGCAGCGCCTGATCGGCAGCGTCGGCATCGGCCATGTG 
CGTTACCCGACCGCCGGCAGCTCCAGCTCGGCGGAGGCGCAGCCGTTCTACGTCAACTCGCCCTACGGCATT 
ACCCTGGCGCACAACGGCAACCTGACCAATGTCGAGCAGTTGGCCAAGGAAATCTACGAATCCGACCTGCGC 
CACGTGAACACCAACTCCGATTCGGAAGTGCTGCTCAACGTGTTCGCCCACGAGCTGGCGGTGCGCAACAAG 
CTGCAGCCCACCGAGGAGGACATCTTCGCCGCGGTTTCCTGCGTGCACGACCGTTGCGTCGGCGGCTACGCG 
GTGGTGGCGATGATCACCGGCCATGGCATCGTCGGTTTCCGCGACCCCAATGCGATCCGTCCGATCGTCTTC 
GGCCAGCGGCATACCGAGAACGGCGTCGAATACATGATCGCCTCCGAGAGCGTGGCCCTGGACGTACTCGGC 
TTCACCCTGATCCGCGACCTGGCGCCGGGCGAGGCGGTGTACATCACCGAGGAAGGCAAGCTCTATACCCGC 
CAGTGCGCGAAGGCGCCGAAGTACGCGCCGTGCATCTTCGAGCACGTCTACCTGGCGCGTCCGGATTCGATC 
ATGGACGGCATCTCGGTGTACAAGGCGCGCCTGCGCATGGGCGAGAAGCTTGCCGACAAGATCCTCCGCGAG 
CGTCCCGACCACGACATCGACGTGGTCATCCCGATCCCCGACACCAGCCGCACCGCGGCGCTGGAACTGGCC 
AACCGGCTCGGCGTGAAGTTCCGCGAGGGCTTCGTCAAGAACCGCTACATCGGCCGCACCTTCATCATGCCC 
GGCCAGGCGGCGCGGAAGAAGTCCGTGCGGCAGAAGCTCAACGCCATCGAGCTGGAGTTCCGCGGCAAGAAC 
GTGATGCTGGTGGATGACTCCATCGTCCGCGGCACCACCTGCAAGCAGATCATCCAGATGGCCCGCGAGGCC 
GGGGCGAAGAACGTCTACTTCTGCTCCGCCGCGCCGGCCGTGCGCTACCCCAACGTCTACGGCATCGACATG 
CCGAGTGCGCACGAGCTGATCGCGCACAATCGCAGCACCGAGGACGTCAGCAAGCTGATCGGCGCCGACTGG 
CTGGTCTACCAGGACCTGCCCGACCTGATCGACGCGGTCGGCGGCGGCAAGATCAAGATCGATCACTTCGAC 
TGCGCCGTGTTCGACGGCGAGTACGTGACCGGCGACGTCAACGAGGCCTACCTGAACCGGATCGAACAGGCG 
CGCAACGATGCGACCAAGGCCAAGAGCCAGGCGGTCAGCGCGATCATCGATCTGTACAACGACTGA