ViewGene
Locus Name:
GeneID:
Location:

GeneID:746
TrivialName: PA14_43460
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_43460
PAO1 Ortholog Locus:PA1628
Sequence Length:1530
Protein Length:509
Start: 3870848
Stop: 3872377
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05017.1|AE004590_8 (AE004590) probable 3-hydroxyacyl-CoA dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 505/508 (99%), Positives = 507/508 (99%)
Sequence:
ATGCCCGATATTCGTTGTATCGCCGTGGTCGGTACCGGTGCGATGGGCACCGGGATCGCCCAGATCGCCGCC
CAGGCCGGCCTGACCGTGTGGCTGTTCGACAGCCGCGCCGAGGCCGCCGCCGAGGCACGCCAGCGCCTGCGG
CAGACCTTCGAGAGTCTATGCGGCAAAGGCAAGCTGAGCGCCGCCGACGCCGAGGCCGCGCAAAACCGGCTG
CGGGTGGCGGAACGCCTGGAACAACTGGGCGACTGCGAACTGGTGGTGGAGGCCATCGTCGAGCGCCTGGAG
GCCAAGCAGGAATTGCTGCGCCGGCTGGAAGCCATAGTCGCGCCGGAGACCATCCTCGCCAGCAATACCTCG
TCGTTGTCGATCACCGCCATCGCCGCCGCCTGCGAGCGGCCGCAGCGGATCGCCGGCTTTCACTTCTTCAAC
CCGGTGCCGCTGATGCGCGTGGTCGAGGTGGTCGACGGCCTGGCCAGCGCTCCGGAAGTCGGCGACGCCCTG
CTCGCCCTCGCCGCGCGCCTGGGCCACCGCGGTGTACGGACCAAGGACAGCCCCGGCTTCATCGTCAACCAT
GCCGGCCGCGCCTATGGCACCGAGGCCCTGCGCATCCTCGGCGAAGGCGTCGCCGAATGCGCCGAGATCGAC
CGTGTGCTGCGCGAGTGCGCCGGCTTCCGCATGGGGCCGTTCGAACTGCTCGATCTCACCGGCCTCGACGTG
TCGCACCCGGTAATGGAATCGATCTACCAGCAGTACTACCAGGAACCGCGCTACCGCCCGCACCCGCTGACC
CGCCAGTTGCTCGCCGCCGGCCGTCTCGGGCGCAAGAGCGGCCAGGGCTTCTATCGCTACGTCGACGGCCAG
CCGCAGGACAAGCCCGGTCCGCAACCGGTGCCGCAGGCCGCCATCCGGCCCCCGGTGTGGCTGGGCTGCGAA
AACGAGGACGACCGCCGCACGCTCGCCGAACTGCTGCATCGCCTCGGCGTCGAGCCGGAAACCGGCGAACGC
CCTTCCGCCGCAGCACTCTGCCTGCTGGCCGCCTGGGGCGAGGACCTGAGCAGCGCCGTGCAGCGCTTCGCC
TGTCCCGCCGAGCGCAGCGTCGGCATCGACCTGCTCTGCGACCTCGAGCGCCGGCGCTGCCTGATGCTCTCG
CCGCTGACCGCCCCGGCCATGCGCGACGCGGCACACGCGCTACTGGCTGGCGACGGCGTCGGGGTCACGGTG
CTGCGCGACAGTCCCGGCTTCGTCGTCCAGCGGGTACTGGCGATGATCGTCAACCTCGCCTGCGACATCGCC
CAGCAGGGCATTGCCAGCGTCGAGGATATCGACCAGGCGGTGCACCTCGGCCTCGGCTATCCCCACGGCCCG
CTGGAATGGGGCGACCGCCTCGGCCCGCGGCGCCTGCTGAGCATTCTCCAGCGCCTGCAGGCGCTTACCGGT
GACCCGCGCTACCGGCCCAGTCCATGGCTGCGCCGACGAGCACAACTGGGCATGTCCCTGCGCGCCGGCGAA
ACCGCCGCCGTAGGCTGA
Translation:
MPDIRCIAVVGTGAMGTGIAQIAAQAGLTVWLFDSRAEAAAEARQRLRQTFESLCGKGKLSAADAEAAQNRL
RVAERLEQLGDCELVVEAIVERLEAKQELLRRLEAIVAPETILASNTSSLSITAIAAACERPQRIAGFHFFN
PVPLMRVVEVVDGLASAPEVGDALLALAARLGHRGVRTKDSPGFIVNHAGRAYGTEALRILGEGVAECAEID
RVLRECAGFRMGPFELLDLTGLDVSHPVMESIYQQYYQEPRYRPHPLTRQLLAAGRLGRKSGQGFYRYVDGQ
PQDKPGPQPVPQAAIRPPVWLGCENEDDRRTLAELLHRLGVEPETGERPSAAALCLLAAWGEDLSSAVQRFA
CPAERSVGIDLLCDLERRRCLMLSPLTAPAMRDAAHALLAGDGVGVTVLRDSPGFVVQRVLAMIVNLACDIA
QQGIASVEDIDQAVHLGLGYPHGPLEWGDRLGPRRLLSILQRLQALTGDPRYRPSPWLRRRAQLGMSLRAGE
TAAVG*
AnnotationID:755GeneID:746
AnnotatorUID: diggins
Modification Date/Time: 2005-02-27 15:03:39
Gene Name:
Confidence Code:3
GeneProduct:putative 3-hydroxyacyl-CoA dehydrogenase
Cell Localization:()
Synonyms:paaH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1250,pfam02737,pfam00725
Secondary Functional Category(ies):carbon compound metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN33427.1|AE014524_2 (AE014524) 3-hydroxyacyl-CoA dehydrogenase family protein [Brucella
           suis 1330]
          Length = 509

 Score =  638 bits (1646), Expect = 0.0
 Identities = 321/501 (64%), Positives = 378/501 (75%)

>gb|AAL54262.1| (AE009734) 3-HYDROXYBUTYRYL-COA DEHYDROGENASE [Brucella melitensis
           16M]
          Length = 565

 Score =  576 bits (1485), Expect = e-163
 Identities = 295/485 (60%), Positives = 352/485 (72%), Gaps = 2/485 (0%)

>emb|CAE34945.1| (BX640451) putative 3-hydroxyacyl-CoA dehydrogenase [Bordetella
           bronchiseptica]
          Length = 504

 Score =  555 bits (1430), Expect = e-157
 Identities = 295/506 (58%), Positives = 360/506 (71%), Gaps = 5/506 (0%)








 
Structural Features:
COG1250, FadB, 3-hydroxyacyl-CoA dehydrogenase [Lipid metabolism]
CD-Length = 307 residues,  94.8% aligned
Score =  336 bits (862), Expect = 4e-93
Identities = 137/291 (47%), Positives = 181/291 (62%)

pfam02737, 3HCDH_N, 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain. 
CD-Length = 185 residues, 100.0% aligned
Score =  183 bits (465), Expect = 6e-47
Identities = 75/185 (40%), Positives = 119/185 (64%)

pfam00725, 3HCDH, 3-hydroxyacyl-CoA dehydrogenase, C-terminal domain.
CD-Length = 97 residues, 100.0% aligned
Score =  122 bits (307), Expect = 1e-28
Identities = 45/98 (45%), Positives = 61/98 (62%), Gaps = 1/98 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1495




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18637




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37291




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:746_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:746
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1300
                           PA1628_tr
                           translation of PA1628
                           509
                           99.21
                           99.60
                           0
                           2590.0
     

     
     
48820
                           gb|AAG05017.1|AE004590_8
                           (AE004590) probable 3-hydroxyacyl-CoA dehydrogenase [Pseudomonas aeruginosa PAO1]
                           509
                           99.21
                           99.60
                           0
                           2590.0
     

     
     
1301
                           PA3590_tr
                           translation of PA3590
                           512
                           65.23
                           74.41
                           5
                           1634.0
     

     
     
48822
                           gb|AAG06978.1|AE004779_10
                           (AE004779) probable hydroxyacyl-CoA dehydrogenase [Pseudomonas aeruginosa PAO1]
                           512
                           65.23
                           74.41
                           5
                           1634.0
     

     
     
48821
                           gb|AAN33427.1|AE014524_2
                           (AE014524) 3-hydroxyacyl-CoA dehydrogenase family protein [Brucella suis 1330]
                           510
                           62.94
                           74.11
                           2
                           1635.0
     

     






      

          
GC ORFID: 43748How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1628Glimmer Score: 

          
Start: 1083061Stop: 1081532

          
Length: 1530


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1628 translation of PA1628Homolog Bit Score 1002.0

          
Other Homologs:  t_PA3590 (ORF: BLASTX 634.0), t_PA1628 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCGATATTCGTTGTATCGCCGTGGTCGGTACCGGTGCGATGGGCACCGGGATCGCCCAGATCGCCGCC 
CAGGCCGGCCTGACCGTGTGGCTGTTCGACAGCCGCGCCGAGGCCGCCGCCGAGGCACGCCAGCGCCTGCGG 
CAGACCTTCGAGAGTCTATGCGGCAAAGGCAAGCTGAGCGCCGCCGACGCCGAGGCCGCGCAAAACCGGCTG 
CGGGTGGCGGAACGCCTGGAACAACTGGGCGACTGCGAACTGGTGGTGGAGGCCATCGTCGAGCGCCTGGAG 
GCCAAGCAGGAATTGCTGCGCCGGCTGGAAGCCATAGTCGCGCCGGAGACCATCCTCGCCAGCAATACCTCG 
TCGTTGTCGATCACCGCCATCGCCGCCGCCTGCGAGCGGCCGCAGCGGATCGCCGGCTTTCACTTCTTCAAC 
CCGGTGCCGCTGATGCGCGTGGTCGAGGTGGTCGACGGCCTGGCCAGCGCTCCGGAAGTCGGCGACGCCCTG 
CTCGCCCTCGCCGCGCGCCTGGGCCACCGCGGTGTACGGACCAAGGACAGCCCCGGCTTCATCGTCAACCAT 
GCCGGCCGCGCCTATGGCACCGAGGCCCTGCGCATCCTCGGCGAAGGCGTCGCCGAATGCGCCGAGATCGAC 
CGTGTGCTGCGCGAGTGCGCCGGCTTCCGCATGGGGCCGTTCGAACTGCTCGATCTCACCGGCCTCGACGTG 
TCGCACCCGGTAATGGAATCGATCTACCAGCAGTACTACCAGGAACCGCGCTACCGCCCGCACCCGCTGACC 
CGCCAGTTGCTCGCCGCCGGCCGTCTCGGGCGCAAGAGCGGCCAGGGCTTCTATCGCTACGTCGACGGCCAG 
CCGCAGGACAAGCCCGGTCCGCAACCGGTGCCGCAGGCCGCCATCCGGCCCCCGGTGTGGCTGGGCTGCGAA 
AACGAGGACGACCGCCGCACGCTCGCCGAACTGCTGCATCGCCTCGGCGTCGAGCCGGAAACCGGCGAACGC 
CCTTCCGCCGCAGCACTCTGCCTGCTGGCCGCCTGGGGCGAGGACCTGAGCAGCGCCGTGCAGCGCTTCGCC 
TGTCCCGCCGAGCGCAGCGTCGGCATCGACCTGCTCTGCGACCTCGAGCGCCGGCGCTGCCTGATGCTCTCG 
CCGCTGACCGCCCCGGCCATGCGCGACGCGGCACACGCGCTACTGGCTGGCGACGGCGTCGGGGTCACGGTG 
CTGCGCGACAGTCCCGGCTTCGTCGTCCAGCGGGTACTGGCGATGATCGTCAACCTCGCCTGCGACATCGCC 
CAGCAGGGCATTGCCAGCGTCGAGGATATCGACCAGGCGGTGCACCTCGGCCTCGGCTATCCCCACGGCCCG 
CTGGAATGGGGCGACCGCCTCGGCCCGCGGCGCCTGCTGAGCATTCTCCAGCGCCTGCAGGCGCTTACCGGT 
GACCCGCGCTACCGGCCCAGTCCATGGCTGCGCCGACGAGCACAACTGGGCATGTCCCTGCGCGCCGGCGAA 
ACCGCCGCCGTAGGCTGA