Modification Date/Time: 2006-04-06 13:01:23
Missense Discrepancy: FALSE
Comments:GeneID6843 (PA14_66870) replaced by 7423 (PA14_66875). Homology: PA5060 [gene=phaF] [prot=polyhydroxyalkanoate synthesis protein PhaF], Length = 309, Score = 577 bits (1486), Expect = e-166, Identities = 304/309 (98%), Positives = 305/309 (98%).
Sequence:
atggctggcaagaagaagagcgaaaaagagtccagttggatcggcgagatcgagaaatactcgcggcagatc
tggctggctggtctgggggcctactcgaaggtcagcaaggacggcagcaagctgttcgagaccttggtgaaa
gacggcgagaaggctgaaaaagaagcgaagtccgatgtggacgcgcaggtcggtgcggcgaaggcttccgcc
cgctctgcgaagagcaaggttgacgaggttcgggatcgtgcgcttggcaagtggagcgagctcgaggaagct
ttcgacaagcgcctgaacagcgccatctcgcgtctcggcgtgccgagccgcaacgaggtgaaggagctgcac
ggcaaggtcgatacgctgaccaggcagatcgagaaactcaccggcgtcagcgtcaagccggcggcgaaggca
gcggccaagcctgcggcgaaaccggctgccaagcccgcggcgaaaaccgcagcggccaagccggcagctaaa
ccggcagccaaggccgccgccaagcctgcggcgaaacccgcggcgaaaaaacctgcagcgaagaccgcggcc
gccaagccggcagccaagcccgccgccaaaccggctgcgaaggccgccgcgaaaccggcggccaagccggca
gccaaggctgcggcgaagcctgctgcgaaacctgccgcagccaagcccgccgcgaagccggcagccaagccg
gccgctgcgaccgccgccaagcccgcggcgaagcctgccgccaagccggctgcgaaaaagcctgcggcgaag
aagccggccgccaagcccgccgcggcgaaaccggccgctcccgccgcgtcttcgagcgcgcccgctgccccc
gccgccacaccggctgccagcgctccggcagcgaacgctccggcgacgccgagcagccagggctga
Translation:
MAGKKKSEKESSWIGEIEKYSRQIWLAGLGAYSKVSKDGSKLFETLVKDGEKAEKEAKSDVDAQVGAAKASA
RSAKSKVDEVRDRALGKWSELEEAFDKRLNSAISRLGVPSRNEVKELHGKVDTLTRQIEKLTGVSVKPAAKA
AAKPAAKPAAKPAAKTAAAKPAAKPAAKAAAKPAAKPAAKKPAAKTAAAKPAAKPAAKPAAKAAAKPAAKPA
AKAAAKPAAKPAAAKPAAKPAAKPAAATAAKPAAKPAAKPAAKKPAAKKPAAKPAAAKPAAPAASSSAPAAP
AATPAASAPAANAPATPSSQG*
AnnotationID:7342 GeneID:7423
Modification Date/Time:
2006-03-13 12:12:20
GeneProduct: polyhydroxyalkanoate synthesis protein PhaF
Cell Localization Confidence Code: 5
Functional Category: (8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code: 5
Secondary Functional Category(ies):
Homology: gi|9951352| gb|AAG08445.1| polyhydroxyalkanoate synthesis protein PhaF [Pseudomonas aeruginosa
PAO1]
gi|15600253| ref|NP_253747.1| polyhydroxyalkanoate synthesis protein PhaF [Pseudomonas aeruginosa
PAO1]
gi|84317693| ref|ZP_00966131.1| COG3937 : Uncharacterized conserved protein [Pseudomonas aeruginosa
C3719]
Length=309
Score = 577 bits (1486), Expect = 3e-163
Identities = 304/309 (98%), Positives = 305/309 (98%), Gaps = 0/309 (0%)
gi|84323853| ref|ZP_00971920.1| COG3937 : Uncharacterized conserved protein [Pseudomonas aeruginosa
2192]
Length=305
Score = 565 bits (1455), Expect = 1e-159
Identities = 300/309 (97%), Positives = 301/309 (97%), Gaps = 4/309 (1%)
Structural Features: pfam05597 , Phasin, Poly(hydroxyalcanoate) granule associated protein (phasin).
Polyhydroxyalkanoates (PHAs) are storage polyesters synthesised by various bacteria as
intracellular carbon and energy reserve material. PHAs are accumulated as water-insoluble
inclusions within the cells. This family consists of the phasins PhaF and PhaI which act as a
transcriptional regulator of PHA biosynthesis genes. PhaF has been proposed
to repress expression
of the phaC1 gene and the phaIF operon..
CD-Length = 132 residues, 100.0% aligned
Score = 135 bits (340), Expect = 8e-33