ViewGene
Locus Name:
GeneID:
Location:

GeneID:742
TrivialName: PA14_35400
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35400
PAO1 Ortholog Locus:PA2256
Sequence Length:1503
Protein Length:500
Start: 3148864
Stop: 3147362
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05644.1|AE004651_6 (AE004651) pyoverdine biosynthesis protein PvcC [Pseudomonas aeruginosa PAO1]
Identities = 498/500 (99%), Positives = 498/500 (99%)
Sequence:
ATGAGCCATTCCGTCCCCAACGCGCTGATGACCGGCGCCGACTATCTCGACAGCCTGCGCGACGGTCGCCAG
GTCTACCTCAACGGCGAACGGGTCGCCGACGTCACCCGGCACAGGGCGTTCCGCAACGCCTGCCGCAGCATC
GCCGGGCTCTACGACGGCCTCCACGGCGAGCAGCGCGACGTCCTCACCCGGGTCGATGCCCAAGGCCGCCGC
AGCCACCGCTTCTTCACCCCCGCGCAGAACGCCGAGGACCTGCTCGGCGCGCGCGAGGCGATCGGCTGCTGG
TCGCGCATGACCTACGGCTTCATGGGGCGTACCCCGGACTACAAGGCGGCGTTCATGTCCGGGCTGGAGGCC
GGCGCCGGCTTCTACGGCGACTACCGGCAGAACGCCTGCGCCTGGCACCGCGCCTTCGCCGACCGCGGGCTG
TTCCTCAACCACGCGATCATCAATCCGCCGCTGGACCGCAGCAAGGCGATCCACGAGATGCGCGACGTGTTC
GTCCACGTCGAACGCGAGACCGACCAGGGTATCGTCGTCAGCGGGGCGAAGATGCTCGCCACCGGCAGCGCC
ATCACCAACGCCACCTTCGTCGCCCCGGTCGCCTCGGCGCAGATGGAGGCCGGCAAGGCCGAGGACTTCGCC
GTTGTGTTCTTCGCCCGCATGGACAACCCCGGCCTGCGCCTGATGTGCCGGCCATCCTACGAGGAGCGCGCG
AGCAGTCCCTTCGACTACCCGCTGTCCAGTCGTTTCGACGAGAACGACAGCGTGCTGCTGTTCGACAAGGCG
CTGATTCCCTGGGAGGACGTCCTGGTCTACCGCGACCTGCGCCGCGCCACCGGCTTCTATGCCGAGTCGGGC
TTCGCCAACCTGTACAACTTCCAGTCCGGCATCCGCCTCGGGGTGAAGCTGGAACTGATGATCGGCCTGCTT
TCCCTCGGCGTGCGCGCCAATGGCACCCAGGGCTTCCGCGGGATACAGGCGGCCCTCGGCGAGCTGATCGCC
CTGCAGCACCTGCTGCAGGCGCTGACCACCGCCATGGCCCGCGACCCGGAGCGCAACGCCGCCGGTTCGGCG
GTGCCGCGCCTGGAGTACGCCAATGCCCTGCGCATCCAGGTGCCGCAGATCTGGAAGCGTGTCCGCGAGTTG
CTGGAAAGCGCCCTCGGCGGCGCCCCGCTGGTCACCGTCTCGGGCGCCGCCGACCTGCGCGACGGCGAGGCG
CGGCGGCTGGTGGACAGCTACTACCGCGGTGCCGAGCTGGAGCCGGAGCAGCGCCTGAAGCTGTTCAAGCTG
ATCTGGGACGCCACCGGCAGCGAGTTCGGCGCACGCCACGCGGTCTATGAGAGCCATTATTCCGGCAATGCC
GAGCAGATCCGCCTCGACTCGCTGGCCTGGGCCAGCCGTCGCGGGCACCTGGCCTACTGCGAGGCGATGGTC
CAGCGCTGCATGGCCGACTACGACATCCACGGCTGGCTACGGGGGCCCTGGCAGCATGACTGA
Translation:
MSHSVPNALMTGADYLDSLRDGRQVYLNGERVADVTRHRAFRNACRSIAGLYDGLHGEQRDVLTRVDAQGRR
SHRFFTPAQNAEDLLGAREAIGCWSRMTYGFMGRTPDYKAAFMSGLEAGAGFYGDYRQNACAWHRAFADRGL
FLNHAIINPPLDRSKAIHEMRDVFVHVERETDQGIVVSGAKMLATGSAITNATFVAPVASAQMEAGKAEDFA
VVFFARMDNPGLRLMCRPSYEERASSPFDYPLSSRFDENDSVLLFDKALIPWEDVLVYRDLRRATGFYAESG
FANLYNFQSGIRLGVKLELMIGLLSLGVRANGTQGFRGIQAALGELIALQHLLQALTTAMARDPERNAAGSA
VPRLEYANALRIQVPQIWKRVRELLESALGGAPLVTVSGAADLRDGEARRLVDSYYRGAELEPEQRLKLFKL
IWDATGSEFGARHAVYESHYSGNAEQIRLDSLAWASRRGHLAYCEAMVQRCMADYDIHGWLRGPWQHD*
AnnotationID:751GeneID:742
AnnotatorUID: diggins
Modification Date/Time: 2005-03-04 07:07:11
Gene Name:pvcC
Confidence Code:1
GeneProduct: pyoverdine biosynthesis protein PvcC
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2368,pfam03241
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAF66546.1|AF140605_2 (AF140605) phenol 2-hydroxylase component A [Geobacillus
           thermoglucosidasius]
          Length = 504

 Score =  465 bits (1197), Expect = e-130
 Identities = 231/494 (46%), Positives = 327/494 (66%), Gaps = 8/494 (1%)

>gb|AAG07478.1|AE004825_7 (AE004825) 4-hydroxyphenylacetate 3-monooxygenase large chain
           [Pseudomonas aeruginosa PAO1]
          Length = 520

 Score =  431 bits (1107), Expect = e-120
 Identities = 221/489 (45%), Positives = 316/489 (64%), Gaps = 9/489 (1%)








 
Structural Features:
 COG2368, COG2368, Aromatic ring hydroxylase 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 493 residues, 100.0% aligned
Score =  556 bits (1433), Expect = 3e-159
Identities = 237/499 (47%), Positives = 306/499 (61%), Gaps = 19/499 (3%)

pfam03241, HpaB, 4-hydroxyphenylacetate 3-hydroxylase family.
CD-Length = 368 residues, 100.0% aligned
Score =  421 bits (1084), Expect = 9e-119
Identities = 161/374 (43%), Positives = 216/374 (57%), Gaps = 8/374 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1487




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18629




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32523




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:742_cdd.html


    		




 
URL:

















    ReferenceID:34445




  
Author/Investigator(s):
        Stintzi A, Johnson Z, Stonehouse M, Ochsner U, Meyer JM, Vasil ML, Poole K.





  
Title:
        The pvc gene cluster of Pseudomonas aeruginosa: role in synthesis of the pyoverdine chromophore and regulation by PtxR and PvdS.




  
PubMed:
        10383985


MedLine:
        




    
Source:
J Bacteriol. 1999 Jul;181(13):4118-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10383985









           
Homologs By Global Alignment 

           

       



     
Gene ID:742
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1292
                           PA2256_tr
                           translation of PA2256
                           500
                           99.60
                           99.60
                           0
                           2598.0
     

     
     
48730
                           gb|AAG05644.1|AE004651_6
                           (AE004651) pyoverdine biosynthesis protein PvcC [Pseudomonas aeruginosa PAO1]
                           500
                           99.60
                           99.60
                           0
                           2598.0
     

     
     
48731
                           gb|AAC21673.1|
                           (AF002222) PvcC [Pseudomonas aeruginosa]
                           500
                           99.40
                           99.40
                           0
                           2594.0
     

     
     
48732
                           gb|AAF66546.1|AF140605_2
                           (AF140605) phenol 2-hydroxylase component A [Geobacillus thermoglucosidasius]
                           512
                           45.11
                           63.86
                           20
                           1200.0
     

     
     
48733
                           gb|AAG07478.1|AE004825_7
                           (AE004825) 4-hydroxyphenylacetate 3-monooxygenase large chain [Pseudomonas aeruginosa PAO1]
                           526
                           42.77
                           61.21
                           32
                           1113.0
     

     






      

          
GC ORFID: 43744How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2256Glimmer Score: 

          
Start: 1805052Stop: 1806554

          
Length: 1503


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2256 translation of PA2256Homolog Bit Score 1005.0

          
Other Homologs:  t_PA4091 (ORF: BLASTX 431.0), t_PA2256 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCATTCCGTCCCCAACGCGCTGATGACCGGCGCCGACTATCTCGACAGCCTGCGCGACGGTCGCCAG 
GTCTACCTCAACGGCGAACGGGTCGCCGACGTCACCCGGCACAGGGCGTTCCGCAACGCCTGCCGCAGCATC 
GCCGGGCTCTACGACGGCCTCCACGGCGAGCAGCGCGACGTCCTCACCCGGGTCGATGCCCAAGGCCGCCGC 
AGCCACCGCTTCTTCACCCCCGCGCAGAACGCCGAGGACCTGCTCGGCGCGCGCGAGGCGATCGGCTGCTGG 
TCGCGCATGACCTACGGCTTCATGGGGCGTACCCCGGACTACAAGGCGGCGTTCATGTCCGGGCTGGAGGCC 
GGCGCCGGCTTCTACGGCGACTACCGGCAGAACGCCTGCGCCTGGCACCGCGCCTTCGCCGACCGCGGGCTG 
TTCCTCAACCACGCGATCATCAATCCGCCGCTGGACCGCAGCAAGGCGATCCACGAGATGCGCGACGTGTTC 
GTCCACGTCGAACGCGAGACCGACCAGGGTATCGTCGTCAGCGGGGCGAAGATGCTCGCCACCGGCAGCGCC 
ATCACCAACGCCACCTTCGTCGCCCCGGTCGCCTCGGCGCAGATGGAGGCCGGCAAGGCCGAGGACTTCGCC 
GTTGTGTTCTTCGCCCGCATGGACAACCCCGGCCTGCGCCTGATGTGCCGGCCATCCTACGAGGAGCGCGCG 
AGCAGTCCCTTCGACTACCCGCTGTCCAGTCGTTTCGACGAGAACGACAGCGTGCTGCTGTTCGACAAGGCG 
CTGATTCCCTGGGAGGACGTCCTGGTCTACCGCGACCTGCGCCGCGCCACCGGCTTCTATGCCGAGTCGGGC 
TTCGCCAACCTGTACAACTTCCAGTCCGGCATCCGCCTCGGGGTGAAGCTGGAACTGATGATCGGCCTGCTT 
TCCCTCGGCGTGCGCGCCAATGGCACCCAGGGCTTCCGCGGGATACAGGCGGCCCTCGGCGAGCTGATCGCC 
CTGCAGCACCTGCTGCAGGCGCTGACCACCGCCATGGCCCGCGACCCGGAGCGCAACGCCGCCGGTTCGGCG 
GTGCCGCGCCTGGAGTACGCCAATGCCCTGCGCATCCAGGTGCCGCAGATCTGGAAGCGTGTCCGCGAGTTG 
CTGGAAAGCGCCCTCGGCGGCGCCCCGCTGGTCACCGTCTCGGGCGCCGCCGACCTGCGCGACGGCGAGGCG 
CGGCGGCTGGTGGACAGCTACTACCGCGGTGCCGAGCTGGAGCCGGAGCAGCGCCTGAAGCTGTTCAAGCTG 
ATCTGGGACGCCACCGGCAGCGAGTTCGGCGCACGCCACGCGGTCTATGAGAGCCATTATTCCGGCAATGCC 
GAGCAGATCCGCCTCGACTCGCTGGCCTGGGCCAGCCGTCGCGGGCACCTGGCCTACTGCGAGGCGATGGTC 
CAGCGCTGCATGGCCGACTACGACATCCACGGCTGGCTACGGGGGCCCTGGCAGCATGACTGA