ViewGene
Locus Name:
GeneID:
Location:

GeneID:74
TrivialName: PA14_11140
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11140
PAO1 Ortholog Locus:PA4078
Sequence Length:2976
Protein Length:991
Start: 971198
Stop: 968223
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07465.1|AE004823_11 (AE004823) probable nonribosomal peptide synthetase [Pseudomonas aeruginosa PAO1]
Identities = 970/991 (97%), Positives = 979/991 (98%)
Sequence:
ATGTCGAAAGATTCTGTTCTTGGCTTATTCAGGCAACACGCAGATACCCATCCCGAACGCCCCGCCCTCGTC
GATCGCGAGCGCTCGTTCAGCTACCGCGAACTCGACCGACTCAGCGACCGGCTGGCCGCCCACCTGGCCAGG
CGCGGCGTCGCCCGGGGCGAGCGGCTGCCCCTGCTGGCCGAACGCTCGGCCGAACTGGTCATCGCCATCCTG
GCGGCCGCCAAGTGCGCAGCGGCCTACGTACCGGTGGACCGTCGGCAACCCGACAGGCGCAAGCAGGAAATC
CTCCGCCAGTGCCAGGCCCCCGTGGCCCTCGCCACCCAGGCCGAGGACCTGCCGGGGCAACCGGTGGAGGTC
ATCGCACAGGTGCTCGCGACGAGCGCGGCGGGTGCCGCGCCGAGGCCGGCGCTCGACGGCAGCGAAGCGCTG
TATGTGATCTTCACCTCGGGCACCACCGGCGAACCCAAGGGCGTGGTGATCGAGAGCCGCTCCCTGAGCAAC
CTCGTGGACTGGCACAACCGGCGCTTCGAGATGGACCAACGGAGCCGCACCACCCTGATGGCCGGCGTCGGC
TTCGACGTTTGCCAATGGGAAATCTGGTCCACCCTGTGCGCAGGCGCCTGCCTCCACCTGGTGCCCGACGAG
GTGCGCCCGGACCCGGCGGCGCTGCTGGCATTCTTCGCCGAGCAGCGGATCAGCCATGCCTTCGCGCCTACC
GTGATGGTCCCTGCGCTGGTGGAGCAACCCGCTCCGCCGTCGCTGGCGCTGCGCTACCTGTTCTGCGCCGGG
GAAAAACTGCCGCCGGTCGCAACCGGCGGGCTGCCCTATACCGTGGTGGATTACTACGGCCCGACCGAAGCC
ACGGTCTTCGCCACCTGCCGCATCGTCGACGCCGAAGCTCATCGGCGGCCCGCCTCAATCGGCACGCCCATC
GACGGTTGCGAGGCATTCATTCTCGACGCCGACGACCGGCCTTGCCATGGCGACCGGCCCGGCGAGCTGAAC
CTGGCGGGCGTCTGCCTGGCGCGCGAATACCTGCGCGACCCGGACATGACCGCCAGGCGCTTCCACTACTCG
CAGGCACTGCGGCGCCGGCTCTATCGCACCGGCGACAAGGCCCGCTGGCTGGCCGACGGCAGCCTGCAGTTC
CTCGGTCGGCTGGACGACCAGGTGAAGATCCGCGGCCACCGCGTCGAACTCGGCGACGTCGAAGCCGCGCTG
CTGCGCCAGCCGGCGATCCATGGCGCGGTGGTGCTGGCGCATGCCGACCCACGCTGCGGTAGCCAGCAATTG
AGCGCCTTCGTGGTCCCCCGCCAGCGGGGCGGCGATGCCAGGGCCGTGCTCGCCGCCATCAAGACCGCCCTG
CGCCAGGAACTGCCCGACTACATGCTGCCCAGCCGCTATCTGTCGCTGGACAGCCTGCCGACCACGGTCAAC
GGCAAGATCGACCGCCAGGCCCTGCGTCGACGCCTGGAGGAACAATGCCAGGAACGACTCGACGAGCAACGC
TTCGGCACTCCCGGCGAACTGCAAGTGGCTCTGGCCTGGCAGGAAGTGCTGGGGCATACCGACTTCGGCCTG
GACGACAGCTTCTTCGAGGTCGGCGGCCATTCCCTGCTGGCCGCCGCCCTGGTGCGCGAACTGAGCCGACGC
TTCGGCAACCGTGCCTACATCCACGACATCTACCGCACCCCGAGCGTGCGCCAACTGGCGGCCAGCCTGGCG
CGGCGCGCCGGCGAAGCGCCGCCGGCGCTGGACAGCGAACCGGCCCAGGAGCTGCAACGGGACGTGCGCCTG
CCCGCCGACGTGGATTTCAGCCGGCCCATGGACACCGCCCAATTGCTCGCGCCACGGCACATCCTGCTCACC
GGCGCCAGCGGGCTGATGGGCGCCCACCTGCTCGCCGAGCTGCTGGCCAGCCGCGAGGCCGACCTGCATTGT
CCGGTCCGTGCGCAAAACGACGCCCACGCCCTCGAACGCCTGCGCCAGGCCGCCCGGCAGCACCGCATCGAA
CTCGCCGAGTCGGACTGGCGACGGGTCAGGGCCTACGCCGCCGACCTCGCAGAACCAGGTTTCGGACTACCG
GCGGAAACCTACCGCGAGCTGGCCGGCAGCGTCGACCAGGTCTTCCATTCCGCCAGCGCGGTGAACTTCATC
CAGCCATACAGCTACATGAAGCGCGACAACGTCGAGGGGCTCGGCCAGGTCCTGCGCTTCTGCGCCAGCGGC
CGCTGCAAGCCGCTGATGCTGCTGTCGAGCATCTCGGTGTACAGCTGGGGCCACCTGCATACCGGCAAGCGC
CTGATGCGCGAGGACGACGACATCGACCAGAACCTGCCGGCGGTGGTCACCGACATGGGCTACGTGCGCAGC
AAATGGGTGATGGAAAAGATCGCCGACCTCGCCGCCGAACGCGGCCTGCCGCTGATGACCTTCCGCCTCGGC
TACGCCACCTGCCACAGCCGTACCGGCGCCCATGCCGACTACCAGTGGTGGAGCCGGCTGGCGCGGACCTGC
CTGGAGTACCGGGCCGTGCCGCTCCTGCGCGAGCTGCGCGAGGGCCTGACCACGGTGGACTACATGGTAGAG
GCGATCAGCGTCATCGCCCGCCAGCCTTCGGCGCTGGGCAAGAAATTCAACCTGGTGCCGAGCATTCCGCGC
TGCCTGACCCTGGACGAGTTCTTCGACCGTCTCGGGCGACGCGCCGGGCGTCCCCTCCGGCAGATGCCGTTC
GACGACTGGGTAAGTCTCTGGGAAGACAATCGCGACGCCCCGCTCTATCCCCTGCTGAGTATGTTCCGCGAC
AACATGTACGCCGGCCGCAGCACCGTCGAGTTGTACCAGGATACCTATCTCTGGGACTGCACCAACGTCGAG
GAACACCTGCGCGGGAGCGCCGTGCGCGAGCCGGAGTTCGACGACCGCCTGCTCGACCTGTACCTCGCCGGC
CTGGGCGGCAGCGCCATGCGGTAA
Translation:
MSKDSVLGLFRQHADTHPERPALVDRERSFSYRELDRLSDRLAAHLARRGVARGERLPLLAERSAELVIAIL
AAAKCAAAYVPVDRRQPDRRKQEILRQCQAPVALATQAEDLPGQPVEVIAQVLATSAAGAAPRPALDGSEAL
YVIFTSGTTGEPKGVVIESRSLSNLVDWHNRRFEMDQRSRTTLMAGVGFDVCQWEIWSTLCAGACLHLVPDE
VRPDPAALLAFFAEQRISHAFAPTVMVPALVEQPAPPSLALRYLFCAGEKLPPVATGGLPYTVVDYYGPTEA
TVFATCRIVDAEAHRRPASIGTPIDGCEAFILDADDRPCHGDRPGELNLAGVCLAREYLRDPDMTARRFHYS
QALRRRLYRTGDKARWLADGSLQFLGRLDDQVKIRGHRVELGDVEAALLRQPAIHGAVVLAHADPRCGSQQL
SAFVVPRQRGGDARAVLAAIKTALRQELPDYMLPSRYLSLDSLPTTVNGKIDRQALRRRLEEQCQERLDEQR
FGTPGELQVALAWQEVLGHTDFGLDDSFFEVGGHSLLAAALVRELSRRFGNRAYIHDIYRTPSVRQLAASLA
RRAGEAPPALDSEPAQELQRDVRLPADVDFSRPMDTAQLLAPRHILLTGASGLMGAHLLAELLASREADLHC
PVRAQNDAHALERLRQAARQHRIELAESDWRRVRAYAADLAEPGFGLPAETYRELAGSVDQVFHSASAVNFI
QPYSYMKRDNVEGLGQVLRFCASGRCKPLMLLSSISVYSWGHLHTGKRLMREDDDIDQNLPAVVTDMGYVRS
KWVMEKIADLAAERGLPLMTFRLGYATCHSRTGAHADYQWWSRLARTCLEYRAVPLLRELREGLTTVDYMVE
AISVIARQPSALGKKFNLVPSIPRCLTLDEFFDRLGRRAGRPLRQMPFDDWVSLWEDNRDAPLYPLLSMFRD
NMYAGRSTVELYQDTYLWDCTNVEEHLRGSAVREPEFDDRLLDLYLAGLGGSAMR*
AnnotationID:84GeneID:74
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 07:07:18
Gene Name:
Confidence Code:3
GeneProduct:putative nonribosomal peptide synthetase
Cell Localization:()
Synonyms:PA4078
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3320,pfam00501,COG0318
Secondary Functional Category(ies):secondary metabolites, biosynthesis, transport, and catabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
COG3320, COG3320, Putative dehydrogenase domain of multifunctional 
non-ribosomal peptide synthetases and related enzymes 
[Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 382 residues, 100.0% aligned
Score =  378 bits (971), Expect = 2e-105
Identities = 139/385 (36%), Positives = 186/385 (48%), Gaps = 15/385 (3%)

pfam00501, AMP-binding, AMP-binding enzyme.
CD-Length = 412 residues, 100.0% aligned
Score =  304 bits (780), Expect = 3e-83
Identities = 152/417 (36%), Positives = 207/417 (49%), Gaps = 33/417 (7%)

COG0318, CaiC, Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II 
[Lipid metabolism / Secondary metabolites biosynthesis, transport, and catabolism]
CD-Length = 534 residues,  97.4% aligned
Score =  257 bits (658), Expect = 4e-69
Identities = 140/528 (26%), Positives = 218/528 (41%), Gaps = 42/528 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:151




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17293




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36825




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:74_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:74
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
149
                           PA4078_tr
                           translation of PA4078
                           991
                           97.88
                           98.78
                           0
                           5041.0
     

     
     
13500
                           gb|AAG07465.1|AE004823_11
                           (AE004823) probable nonribosomal peptide synthetase [Pseudomonas aeruginosa PAO1]
                           991
                           97.88
                           98.78
                           0
                           5041.0
     

     
     
13502
                           gb|AAO91946.1|
                           (AY228241) tolaasin [Pseudomonas tolaasii]
                           1012
                           13.73
                           18.57
                           687
                           505.0
     

     
     
150
                           PA2400_tr
                           translation of PA2400
                           1713
                           12.55
                           17.57
                           1118
                           653.0
     

     
     
13501
                           gb|AAG05788.1|AE004666_4
                           (AE004666) probable non-ribosomal peptide synthetase [Pseudomonas aeruginosa PAO1]
                           1713
                           12.55
                           17.57
                           1118
                           653.0
     

     






      

          
GC ORFID: 43076How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4078Glimmer Score: 

          
Start: 3982739Stop: 3985714

          
Length: 2976


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4078 translation of PA4078Homolog Bit Score 1946.0

          
Other Homologs:  t_PA4078 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGAAAGATTCTGTTCTTGGCTTATTCAGGCAACACGCAGATACCCATCCCGAACGCCCCGCCCTCGTC 
GATCGCGAGCGCTCGTTCAGCTACCGCGAACTCGACCGACTCAGCGACCGGCTGGCCGCCCACCTGGCCAGG 
CGCGGCGTCGCCCGGGGCGAGCGGCTGCCCCTGCTGGCCGAACGCTCGGCCGAACTGGTCATCGCCATCCTG 
GCGGCCGCCAAGTGCGCAGCGGCCTACGTACCGGTGGACCGTCGGCAACCCGACAGGCGCAAGCAGGAAATC 
CTCCGCCAGTGCCAGGCCCCCGTGGCCCTCGCCACCCAGGCCGAGGACCTGCCGGGGCAACCGGTGGAGGTC 
ATCGCACAGGTGCTCGCGACGAGCGCGGCGGGTGCCGCGCCGAGGCCGGCGCTCGACGGCAGCGAAGCGCTG 
TATGTGATCTTCACCTCGGGCACCACCGGCGAACCCAAGGGCGTGGTGATCGAGAGCCGCTCCCTGAGCAAC 
CTCGTGGACTGGCACAACCGGCGCTTCGAGATGGACCAACGGAGCCGCACCACCCTGATGGCCGGCGTCGGC 
TTCGACGTTTGCCAATGGGAAATCTGGTCCACCCTGTGCGCAGGCGCCTGCCTCCACCTGGTGCCCGACGAG 
GTGCGCCCGGACCCGGCGGCGCTGCTGGCATTCTTCGCCGAGCAGCGGATCAGCCATGCCTTCGCGCCTACC 
GTGATGGTCCCTGCGCTGGTGGAGCAACCCGCTCCGCCGTCGCTGGCGCTGCGCTACCTGTTCTGCGCCGGG 
GAAAAACTGCCGCCGGTCGCAACCGGCGGGCTGCCCTATACCGTGGTGGATTACTACGGCCCGACCGAAGCC 
ACGGTCTTCGCCACCTGCCGCATCGTCGACGCCGAAGCTCATCGGCGGCCCGCCTCAATCGGCACGCCCATC 
GACGGTTGCGAGGCATTCATTCTCGACGCCGACGACCGGCCTTGCCATGGCGACCGGCCCGGCGAGCTGAAC 
CTGGCGGGCGTCTGCCTGGCGCGCGAATACCTGCGCGACCCGGACATGACCGCCAGGCGCTTCCACTACTCG 
CAGGCACTGCGGCGCCGGCTCTATCGCACCGGCGACAAGGCCCGCTGGCTGGCCGACGGCAGCCTGCAGTTC 
CTCGGTCGGCTGGACGACCAGGTGAAGATCCGCGGCCACCGCGTCGAACTCGGCGACGTCGAAGCCGCGCTG 
CTGCGCCAGCCGGCGATCCATGGCGCGGTGGTGCTGGCGCATGCCGACCCACGCTGCGGTAGCCAGCAATTG 
AGCGCCTTCGTGGTCCCCCGCCAGCGGGGCGGCGATGCCAGGGCCGTGCTCGCCGCCATCAAGACCGCCCTG 
CGCCAGGAACTGCCCGACTACATGCTGCCCAGCCGCTATCTGTCGCTGGACAGCCTGCCGACCACGGTCAAC 
GGCAAGATCGACCGCCAGGCCCTGCGTCGACGCCTGGAGGAACAATGCCAGGAACGACTCGACGAGCAACGC 
TTCGGCACTCCCGGCGAACTGCAAGTGGCTCTGGCCTGGCAGGAAGTGCTGGGGCATACCGACTTCGGCCTG 
GACGACAGCTTCTTCGAGGTCGGCGGCCATTCCCTGCTGGCCGCCGCCCTGGTGCGCGAACTGAGCCGACGC 
TTCGGCAACCGTGCCTACATCCACGACATCTACCGCACCCCGAGCGTGCGCCAACTGGCGGCCAGCCTGGCG 
CGGCGCGCCGGCGAAGCGCCGCCGGCGCTGGACAGCGAACCGGCCCAGGAGCTGCAACGGGACGTGCGCCTG 
CCCGCCGACGTGGATTTCAGCCGGCCCATGGACACCGCCCAATTGCTCGCGCCACGGCACATCCTGCTCACC 
GGCGCCAGCGGGCTGATGGGCGCCCACCTGCTCGCCGAGCTGCTGGCCAGCCGCGAGGCCGACCTGCATTGT 
CCGGTCCGTGCGCAAAACGACGCCCACGCCCTCGAACGCCTGCGCCAGGCCGCCCGGCAGCACCGCATCGAA 
CTCGCCGAGTCGGACTGGCGACGGGTCAGGGCCTACGCCGCCGACCTCGCAGAACCAGGTTTCGGACTACCG 
GCGGAAACCTACCGCGAGCTGGCCGGCAGCGTCGACCAGGTCTTCCATTCCGCCAGCGCGGTGAACTTCATC 
CAGCCATACAGCTACATGAAGCGCGACAACGTCGAGGGGCTCGGCCAGGTCCTGCGCTTCTGCGCCAGCGGC 
CGCTGCAAGCCGCTGATGCTGCTGTCGAGCATCTCGGTGTACAGCTGGGGCCACCTGCATACCGGCAAGCGC 
CTGATGCGCGAGGACGACGACATCGACCAGAACCTGCCGGCGGTGGTCACCGACATGGGCTACGTGCGCAGC 
AAATGGGTGATGGAAAAGATCGCCGACCTCGCCGCCGAACGCGGCCTGCCGCTGATGACCTTCCGCCTCGGC 
TACGCCACCTGCCACAGCCGTACCGGCGCCCATGCCGACTACCAGTGGTGGAGCCGGCTGGCGCGGACCTGC 
CTGGAGTACCGGGCCGTGCCGCTCCTGCGCGAGCTGCGCGAGGGCCTGACCACGGTGGACTACATGGTAGAG 
GCGATCAGCGTCATCGCCCGCCAGCCTTCGGCGCTGGGCAAGAAATTCAACCTGGTGCCGAGCATTCCGCGC 
TGCCTGACCCTGGACGAGTTCTTCGACCGTCTCGGGCGACGCGCCGGGCGTCCCCTCCGGCAGATGCCGTTC 
GACGACTGGGTAAGTCTCTGGGAAGACAATCGCGACGCCCCGCTCTATCCCCTGCTGAGTATGTTCCGCGAC 
AACATGTACGCCGGCCGCAGCACCGTCGAGTTGTACCAGGATACCTATCTCTGGGACTGCACCAACGTCGAG 
GAACACCTGCGCGGGAGCGCCGTGCGCGAGCCGGAGTTCGACGACCGCCTGCTCGACCTGTACCTCGCCGGC 
CTGGGCGGCAGCGCCATGCGGTAA