ViewGene

Locus Name:
GeneID:
Location:

GeneID: 7388

TrivialName: PA14_24445

AnnotatorUID: danlee

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_24445

PAO1 Ortholog Locus: PA3068

Sequence Length: 4863

Protein Length: 1620

Start: 2130044

Stop: 2134906

Strand: +

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Replaces PA14_24450 (GeneID 6248) and PA14_24460 (GeneID 6247). Homology: PA3068 [gene=gdhB] [prot=NAD-dependent glutamate dehydrogenase], Length = 1620, Score = 3206 bits (8311), Expect = 0.0, Identities = 1618/1620 (99%), Positives = 1619/1620 (99%)

Sequence:

atggcgttcttcaccgcggccagcaaagccgatttccagcatcaactgcagacggcactggcgcagcacctc
ggcgacaaggcgttaccacaagtcacgctgttcgccgaacagttcttcagcctgatctccctcgatgaactg
acccagcgtcggctttccgacctggtcggttgcaccctttccgcctggcgcctgctggagcgcttcgaccgc
gaccagcccgaagtccgcgtatacaaccccgactacgagaagcacggctggcaatccacccacaccgcggtg
gaagtgctgcacccggacctgccgttcctggtcgactcggtgcgcatggaactgaaccgtcgcggctacagc
atccataccctgcagaccaacgtcctcagcgtgcgccgctcggccaagggcgagctgaaggaaatcctgccc
aagggtagccaaggcaaggacgtcagccaggaatcgctgatgtacctggagatcgaccgttgcgcccacgcc
ggcgaactgcgggcgctggagaaggccatcctggaagtgctcggtgaagtacgcgtgaccgtcgccgacttc
gaaccgatgaaggccaaggcccgcgaactgctgacctggcttggcaaggccaagctgaaggtgccggcggag
gaactgaaggaagtgcgcagctatctcgaatggctgctggacaaccacttcaccttcctcggctacgaggaa
ttcagcgtcgccgacgaggccgacggcgggcgcatggtctacgacgagaaatccttcctcggcctgacccgt
ctgctgcgtgccggcctgagcaaggatgacctgcacatcgaggactatgccgtcgcctacctgcgcgaaccg
gtcctgctgtccttcgccaaggctgcccatccgagccgcgtgcaccgcccggcctacccggactacgtgtcg
atccgcgagctggacggcaagggccgggtgatccgcgagtgccgcttcatgggcctgttcacctcctcggtg
tacaacgagagcgtcaacgacattcccttcatccgcggcaaggtcgccgaagtgatgcggcgctccggtttc
gacaccaaggcccacctcggcaaggaactggcccaggtgctggaagtactgccgcgcgacgacctgttccag
accccggtggacgagctgttcagcaccgccctggcgatcgtccgcatccaggagcgcaacaagatccgcgtg
ttcctgcgcaaggacccctacggtcgcttctgctactgcctggcctacgtgccgcgcgacgtgtattccacc
gagacgcgcctgaagatccagcaggtcctgatggagcgcctgcaagccagcgactgcgagttctggactttc
ttctccgagtcggtgctggcccgcgtgcagttcatcctccgcgtcgacccgaagtcgcgcatcgacatcgac
ccggcgcgcctggaggaagaagtgatccaggcctgccgttcctggcaggacgactacagcagcctggtggtg
gaaaacctcggcgaggccaagggcaccaacgtcctggccgacttcccgaaaggcttcccggccggctatcgc
gagcgcttcgccccgcacttcgcggtggtcgacctgcagcacctgctcagcctgtccgagcagcgcccgctg
gtgatgagcttctaccagccgctggcgcagggcgagcagcaactgcactgcaagctgtaccacgccgacacc
ccgctggccctgtccgacgtcctgccgatcctggagaacctcggcctgcgcgtgctcggcgaattcccctac
cgcctgcgccaccagaacggccgcgaatactggatccacgacttcgccttcacctacgccgaagggctggac
gtggacatccagcagctcaacgagatcctccaggacgccttcgtccacatcgtcagcggcgacgccgagaac
gacgccttcaaccgcctggtgctgaccgccaacctgccgtggcgcgacgtcgccctgctgcgtgcctacgcg
cgttacctgaagcagatccgcctgggcttcgacctcggctacatcgccagtgcgctgaatgcccacaccgac
atcgcccgcgagctggtgcggctgttcaagacccgcttctacctggcgcgcaaactcaccgcggaagacctg
gaagacaagcaacagaagctggagcaggcgatcctcggtgccctcgacgaagtccaggtgctcaacgaggac
cgcatcctgcggcgctacctcgacctgatcaaggcaaccctgcggaccaacttctaccagcccgacggcaac
ggccagaacaagagctacttcagcttcaagttcaacccgaaggccatcccggagctgcccaggccggtgccc
aagtacgagatcttcgtctactcgccgcgcgtcgagggcgtgcacctgcgcggcggcaaggtggctcgcggc
ggcttgcgctggtcggaccgcgaggaagacttccgcaccgaggtgctcggcctggtcaaggcccagcaggtg
aagaacgcggtgatcgtgccggtcggcgccaagggcggcttcgtcccgcgccgcctgccgcttggcggtagc
cgcgacgagatccaggccgaggcgatcgcctgctaccggatcttcatttccggcctgctcgacatcaccgac
aacctcaaggaaggcgaagtggtgccgccggccaacgtggtccgccacgacgaggacgatccctacctggtg
gtcgccgcggacaagggcacggcgaccttctccgacatcgccaacggcatcgccgccgagtacggtttctgg
ctcggcgacgccttcgcctcgggcggctccgccggctacgaccacaagggcatggggattaccgccaagggc
gcctgggtcagcgtgcaacggcacttccgcgaacgcggcatcgacgtgcagaaggacaacatcagcgtcatc
ggcatcggcgacatggccggcgacgtgttcggcaacggcctgctgatgtcggacaagctgcaactggtggcc
gccttcaaccacatgcatatcttcatcgacccgaacccggacgccgccagcagcttcgtcgagcgccagcgc
ctgttcaacctgccgcgttcgagctgggccgactacgacgccaagctgatctccgccggcggcgggatcttc
ctgcgcagcgccaagagcatcgccatcaccccggaaatgcaggcgcgcttcgacatccaggccgacaggctg
gcgcccaccgagctgatccacgcgctgctcaaggcgccggtcgacctgctctggaacggcggtatcggcacc
tacgtgaagtcgagcaaggaaacccacgccgatgtcggcgacaaggccaacgacggtctgcgcgtcgacggc
cgcgagctgcgcgcgaaggtggtgggcgagggcggcaacctgggcatgacccaactggcgcgggtcgagttc
ggcctgcacggcggtgccaacaacaccgatttcatcgacaacgtcggtggcgtggactgctccgaccatgag
gtcaacatcaagatcctgctcaacgaagtggtgcaggccggcgacatgaccgagaagcagcgcaacgcgctg
ctggtgaagatgaccgacgcggtcggcgccctggtgctgggcaacaactacaagcagacccaggcgctgtcc
ctggcgcaacgccgcgcccgcgagcggatcgccgaatacaagcggctgatgggcgacctcgaggcgcgcggc
aagctcgatcgcgccctggagttcctgccttccgacgaggaactggcggaacgcatcagtgccggccagggg
ctgacccgcgccgagctgtcggtgctgatctcctacagcaagatcgacctcaaggaatcgctgctgaaatcg
ctggtgccggacgacgactacctgacccgcgacatggagaccgccttcccggcattgctggcggagaagttc
ggcgacgccatgcgccgccatcgcctgaagcgcgagatcgtcagcacgcagatcgccaacgacctggtcaac
cacatgggcatcaccttcgtgcagcgcctgaaggagtccaccggcatgagcgcggccaacgtcgccggggcc
tacgtgatcgttcgcgacgtgttccacctgccgcactggttccgccagatcgagaacctcgactaccaggtg
cccgctgatatccagctgaccctgatggacgaactgatgcgtctcggtcgccgcgccacccgctggttcctc
cgcagccgccgcaacgagctggacgcggcgcgcgacgtggcccacttcggcccgcgcatcgccgccctcggc
ctgaagctcaacgagctgctcgaagggccgacccgcgagctgtggcaggcgcgctaccagacctacgtcgac
gccggcgtaccggaactgctggcacgcatggtggccggcaccagccacctgtatacgctgctgccgatcatc
gaggcgtccgacgtgaccggccaggacaccgcggaagtggccaaggcctatttcgccgtgggcagcgcgctg
gaccttacctggtacctgcaacagatcaccaacctgccggtggagaacaactggcaggcgctggcccgcgag
gcgttccgcgacgacctcgactggcagcagcgggcgatcaccgtctcggtgttgcagatgcaggacggaccg
aaggaggtcgaggctcgcgtcggcctgtggctggaacaacacctgccgctggtcgagcgctggcgggcgatg
ctggtcgaactgcgtgccgcgtcgggtaccgactacgccatgtacgccgtggccaaccgcgagctgatggac
ctggcgcagagcagccagcacggtgtctgcatcccctga

Translation:

MAFFTAASKADFQHQLQTALAQHLGDKALPQVTLFAEQFFSLISLDELTQRRLSDLVGCTLSAWRLLERFDR
DQPEVRVYNPDYEKHGWQSTHTAVEVLHPDLPFLVDSVRMELNRRGYSIHTLQTNVLSVRRSAKGELKEILP
KGSQGKDVSQESLMYLEIDRCAHAGELRALEKAILEVLGEVRVTVADFEPMKAKARELLTWLGKAKLKVPAE
ELKEVRSYLEWLLDNHFTFLGYEEFSVADEADGGRMVYDEKSFLGLTRLLRAGLSKDDLHIEDYAVAYLREP
VLLSFAKAAHPSRVHRPAYPDYVSIRELDGKGRVIRECRFMGLFTSSVYNESVNDIPFIRGKVAEVMRRSGF
DTKAHLGKELAQVLEVLPRDDLFQTPVDELFSTALAIVRIQERNKIRVFLRKDPYGRFCYCLAYVPRDVYST
ETRLKIQQVLMERLQASDCEFWTFFSESVLARVQFILRVDPKSRIDIDPARLEEEVIQACRSWQDDYSSLVV
ENLGEAKGTNVLADFPKGFPAGYRERFAPHFAVVDLQHLLSLSEQRPLVMSFYQPLAQGEQQLHCKLYHADT
PLALSDVLPILENLGLRVLGEFPYRLRHQNGREYWIHDFAFTYAEGLDVDIQQLNEILQDAFVHIVSGDAEN
DAFNRLVLTANLPWRDVALLRAYARYLKQIRLGFDLGYIASALNAHTDIARELVRLFKTRFYLARKLTAEDL
EDKQQKLEQAILGALDEVQVLNEDRILRRYLDLIKATLRTNFYQPDGNGQNKSYFSFKFNPKAIPELPRPVP
KYEIFVYSPRVEGVHLRGGKVARGGLRWSDREEDFRTEVLGLVKAQQVKNAVIVPVGAKGGFVPRRLPLGGS
RDEIQAEAIACYRIFISGLLDITDNLKEGEVVPPANVVRHDEDDPYLVVAADKGTATFSDIANGIAAEYGFW
LGDAFASGGSAGYDHKGMGITAKGAWVSVQRHFRERGIDVQKDNISVIGIGDMAGDVFGNGLLMSDKLQLVA
AFNHMHIFIDPNPDAASSFVERQRLFNLPRSSWADYDAKLISAGGGIFLRSAKSIAITPEMQARFDIQADRL
APTELIHALLKAPVDLLWNGGIGTYVKSSKETHADVGDKANDGLRVDGRELRAKVVGEGGNLGMTQLARVEF
GLHGGANNTDFIDNVGGVDCSDHEVNIKILLNEVVQAGDMTEKQRNALLVKMTDAVGALVLGNNYKQTQALS
LAQRRARERIAEYKRLMGDLEARGKLDRALEFLPSDEELAERISAGQGLTRAELSVLISYSKIDLKESLLKS
LVPDDDYLTRDMETAFPALLAEKFGDAMRRHRLKREIVSTQIANDLVNHMGITFVQRLKESTGMSAANVAGA
YVIVRDVFHLPHWFRQIENLDYQVPADIQLTLMDELMRLGRRATRWFLRSRRNELDAARDVAHFGPRIAALG
LKLNELLEGPTRELWQARYQTYVDAGVPELLARMVAGTSHLYTLLPIIEASDVTGQDTAEVAKAYFAVGSAL
DLTWYLQQITNLPVENNWQALAREAFRDDLDWQQRAITVSVLQMQDGPKEVEARVGLWLEQHLPLVERWRAM
LVELRAASGTDYAMYAVANRELMDLAQSSQHGVCIP*

AnnotationID:7300 GeneID:7388

AnnotatorUID: danlee

Modification Date/Time: 2006-03-10 18:06:40

Gene Name: gdhB

Confidence Code: 2

GeneProduct: NAD-dependent glutamate dehydrogenase

Cell Localization: (1) Cytoplasmic

Synonyms: PA3068

Cell Localization Confidence Code: 3

MolecularFunction:

Functional Category: (2) Amino acid biosynthesis and metabolism

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: pfam05088,COG2902

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Homology:

gi|12484094|gb|AAG53963.1|  NAD(+)-dependent glutamate dehydrogenase [Pseudomonas aeruginosa]
 gi|9949175|gb|AAG06456.1|  NAD-dependent glutamate dehydrogenase [Pseudomonas aeruginosa 
PAO1]
 gi|15598264|ref|NP_251758.1|  hypothetical protein PA3068 [Pseudomonas aeruginosa PAO1]
 gi|84326311|ref|ZP_00974339.1|  COG2902: NAD-specific glutamate dehydrogenase [Pseudomonas aeruginosa 
2192]
 gi|84320234|ref|ZP_00968618.1|  COG2902: NAD-specific glutamate dehydrogenase [Pseudomonas aeruginosa 
C3719]
Length=1620

 Score = 3206 bits (8311),  Expect = 0.0
 Identities = 1618/1620 (99%), Positives = 1619/1620 (99%), Gaps = 0/1620 (0%)

Structural Features:

pfam05088, Bac_GDH, Bacterial NAD-glutamate dehydrogenase. This family consists of several bacterial 
proteins which are closely related to NAD-glutamate dehydrogenase found in Streptomyces clavuligerus. 
Glutamate dehydrogenases (GDHs) are a broadly distributed group of enzymes that catalyse the 
reversible oxidative deamination of glutamate to ketoglutarate and ammonia..
             CD-Length = 1528 residues, 100.0% aligned
             Score = 2324 bits (6024), Expect = 0.0

COG2902, NAD-specific glutamate dehydrogenase [Amino acid transport and metabolism].
             CD-Length = 1592 residues,  98.2% aligned

Genomic Context:

Comment:

Replaces PA14_24450 (GeneID 6248) and PA14_24460 (GeneID 6247).