ViewGene
Locus Name:
GeneID:
Location:

GeneID:734
TrivialName: PA14_01720
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_01720
PAO1 Ortholog Locus:PA0140
Sequence Length:1566
Protein Length:521
Start: 156872
Stop: 158437
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03530.1|AE004452_2 (AE004452) alkyl hydroperoxide reductase subunit F [Pseudomonas aeruginosa PAO1]
Identities = 518/521 (99%), Positives = 520/521 (99%)
Sequence:
ATGTTGGACGCCAATCTTAAAACCCAGTTGAAGGCCTACCTGGAAAAGGTCAGCCAGCCGTTCGAGATCGTC
GCGTCCCTCGATGACAGCGACAAATCCCGCGAGCTGCTGGGCCTGCTGCAAGACATCGTCGGCCTGACCGAC
AAGATCACCCTGAAGACCGACGGCAGCGATGCGCGCAAACCGTCGTTCTCGCTGAACCGCCCCGGTGCGGAC
ATCGGCCTGCGCTTCGCCGGTATCCCCATGGGCCACGAATTCACCTCGCTGGTGCTGGCCCTGCTGCAGGTA
GGCGGCCACCCCTCCAAGCTCGACGCGGACGTGATCGAGCAGGTCAAGGGCATCGAGGGCACCTTCGAGTTC
GAAACCTACTTCTCGCTGTCCTGCCAGAACTGCCCGGACGTGGTCCAGGCGCTGAACCTGATGGCCGTGCTC
AACCCGAACATCCGTCACGTCGCCATCGACGGCGCGCTGTTCCAGGATGAGGTCGAGGCGCGCCAGATCATG
TCGGTGCCGAGCATCTACCTCAACGGCGAAGTCTTCGGCCAGGGCCGCATGGGCGTGGAAGAGATCCTCGCC
AAGATCGACACCGGCGCCGCCGCCCGCGACGCCGAGAAGCTGACCGCCCGCGACGCCTTCGACGTACTGGTG
GTCGGCGGTGGTCCGGCCGGCGCCGCGGCGGCGATCTACGCCGCGCGCAAGGGCATTCGCACCGGCGTCGCC
GCCGAGCGCTTCGGCGGCCAGGTGCTGGACACCATGGCCATCGAGAACTTCATCTCGGTGCAGGAGACCGAC
GGGCCGAAGCTGGCGCGGGCGCTGGAAGAGCACGTGCGTCACTACGAGGTCGACATCATGAACCTGCAGCGC
GCCAGCAAGCTGGTTCCGGCGAAGAACACCGGCGAGTTGCACGAGGTGCAGTTCGAGAGCGGCGGCAGCCTC
AAGGCCAAGACCCTGATCCTCGCCACCGGCGCTCGCTGGCGCGAAATGGGCGTGCCCGGCGAGCAGGAATAC
AAGGCCAAGGGCGTGTGCTTCTGCCCGCACTGCGACGGCCCGCTGTTCAAGGGCAAGCGCGTGGCGGTGATC
GGCGGCGGCAACTCTGGCGTCGAGGCGGCCATCGACCTGGCCGGCATCGTCGCCCATGTGACCCTGCTGGAA
TTCGACAGCAAGTTGCGCGCCGATGCCGTGCTGCAACGCAAGCTCTACAGCCTGCCGAACGTCGAGGTGATC
ACCAGTGCCCTGACCAGCGAAGTGAAGGGCGACGGCCAGAAGGTCACCGGCCTGGTGTACAAGGACCGCAAC
TCCGAGGAGTTCAAGTCGATCGAGCTGGAAGGCATCTTCGTGCAGATCGGCCTGCTGCCCAACACCGAGTGG
CTCAAGGGCAGCGTCGAGCTGTCGCCGCGTGGCGAGATCATCGTCGACGCCCGTGGCGAGACCTCGCTGCCC
GGCATCTTCGCCGCCGGCGACGTGACCACGGTGCCGTACAAGCAGATCGTGATCGCCGTGGGCGAGGGCGCC
AAGGCTTCGCTGAGCGCCTTCGACCACCTGATCCGGACCTCCGCGCCGGAGTGA
Translation:
MLDANLKTQLKAYLEKVSQPFEIVASLDDSDKSRELLGLLQDIVGLTDKITLKTDGSDARKPSFSLNRPGAD
IGLRFAGIPMGHEFTSLVLALLQVGGHPSKLDADVIEQVKGIEGTFEFETYFSLSCQNCPDVVQALNLMAVL
NPNIRHVAIDGALFQDEVEARQIMSVPSIYLNGEVFGQGRMGVEEILAKIDTGAAARDAEKLTARDAFDVLV
VGGGPAGAAAAIYAARKGIRTGVAAERFGGQVLDTMAIENFISVQETDGPKLARALEEHVRHYEVDIMNLQR
ASKLVPAKNTGELHEVQFESGGSLKAKTLILATGARWREMGVPGEQEYKAKGVCFCPHCDGPLFKGKRVAVI
GGGNSGVEAAIDLAGIVAHVTLLEFDSKLRADAVLQRKLYSLPNVEVITSALTSEVKGDGQKVTGLVYKDRN
SEEFKSIELEGIFVQIGLLPNTEWLKGSVELSPRGEIIVDARGETSLPGIFAAGDVTTVPYKQIVIAVGEGA
KASLSAFDHLIRTSAPE*
AnnotationID:743GeneID:734
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 06:06:56
Gene Name:ahpF
Confidence Code:2
GeneProduct:alkyl hydroperoxide reductase subunit F
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3634,pfam00070
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:1.6.4.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68052.1|AE016437_9 (AE016783) alkyl hydroperoxide reductase, F subunit [Pseudomonas
           putida KT2440]
          Length = 520

 Score =  841 bits (2173), Expect = 0.0
 Identities = 424/520 (81%), Positives = 470/520 (90%), Gaps = 1/520 (0%)

>gb|AAC45426.1| (U94336) alkyl hydroperoxide reductase subunit F [Xanthomonas
           campestris]
          Length = 530

 Score =  781 bits (2016), Expect = 0.0
 Identities = 394/521 (75%), Positives = 458/521 (87%), Gaps = 2/521 (0%)

>gb|AAG54941.1|AE005240_7 (AE005240) alkyl hydroperoxide reductase, F52a subunit;
           detoxification of hydroperoxides [Escherichia coli
           O157:H7 EDL933]
          Length = 531

 Score =  689 bits (1779), Expect = 0.0
 Identities = 342/518 (66%), Positives = 422/518 (81%), Gaps = 1/518 (0%)








 
Structural Features:
COG3634, AhpF, Alkyl hydroperoxide reductase, large subunit 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 520 residues, 100.0% aligned
Score =  908 bits (2348), Expect = 0.0
Identities = 396/520 (76%), Positives = 449/520 (86%)

pfam00070, Pyr_redox, Pyridine nucleotide-disulphide oxidoreductase.
CD-Length = 288 residues, 100.0% aligned
Score =  177 bits (451), Expect = 2e-45
Identities = 89/293 (30%), Positives = 138/293 (47%), Gaps = 12/293 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1471




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18613




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33519




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:734_cdd.html


    		




 
URL:

















    ReferenceID:35497




  
Author/Investigator(s):
        Antelmann H, Engelmann S, Schmid R, Hecker M.





  
Title:
        General and oxidative stress responses in Bacillus subtilis: cloning, expression, and mutation of the alkyl hydroperoxide reductase operon.




  
PubMed:
        8932314


MedLine:
        




    
Source:
J Bacteriol. 1996 Nov;178(22):6571-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8932314

















    ReferenceID:35498




  
Author/Investigator(s):
        Rocha ER, Smith CJ.





  
Title:
         
Role of the alkyl hydroperoxide reductase (ahpCF) gene in oxidative stress defense of the obligate Anaerobe bacteroides fragilis.




  
PubMed:
        10482511


MedLine:
        




    
Source:
J Bacteriol. 1999 Sep;181(18):5701-10.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10482511









           
Homologs By Global Alignment 

           

       



     
Gene ID:734
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1280
                           PA0140_tr
                           translation of PA0140
                           521
                           99.42
                           99.80
                           0
                           2610.0
     

     
     
48371
                           gb|AAG03530.1|AE004452_2
                           (AE004452) alkyl hydroperoxide reductase subunit F [Pseudomonas aeruginosa PAO1]
                           521
                           99.42
                           99.80
                           0
                           2610.0
     

     
     
48372
                           gb|AAN68052.1|AE016437_9
                           (AE016783) alkyl hydroperoxide reductase, F subunit [Pseudomonas putida KT2440]
                           521
                           81.38
                           90.21
                           1
                           2174.0
     

     
     
48373
                           dbj|BAA31469.1|
                           (AB010689) AhpF [Pseudomonas putida]
                           521
                           81.38
                           90.21
                           1
                           2174.0
     

     
     
48374
                           gb|AAO56594.1|
                           (AE016867) alkyl hydroperoxide reductase, subunit F [Pseudomonas syringae pv. tomato str. DC3000]
                           521
                           79.07
                           88.67
                           1
                           2110.0
     

     






      

          
GC ORFID: 43736How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0140Glimmer Score: 

          
Start: 5958211Stop: 5959776

          
Length: 1566


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0140 translation of PA0140Homolog Bit Score 1009.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7276 (ORF: BLASTX 823.0), t_PA0140 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTGGACGCCAATCTTAAAACCCAGTTGAAGGCCTACCTGGAAAAGGTCAGCCAGCCGTTCGAGATCGTC 
GCGTCCCTCGATGACAGCGACAAATCCCGCGAGCTGCTGGGCCTGCTGCAAGACATCGTCGGCCTGACCGAC 
AAGATCACCCTGAAGACCGACGGCAGCGATGCGCGCAAACCGTCGTTCTCGCTGAACCGCCCCGGTGCGGAC 
ATCGGCCTGCGCTTCGCCGGTATCCCCATGGGCCACGAATTCACCTCGCTGGTGCTGGCCCTGCTGCAGGTA 
GGCGGCCACCCCTCCAAGCTCGACGCGGACGTGATCGAGCAGGTCAAGGGCATCGAGGGCACCTTCGAGTTC 
GAAACCTACTTCTCGCTGTCCTGCCAGAACTGCCCGGACGTGGTCCAGGCGCTGAACCTGATGGCCGTGCTC 
AACCCGAACATCCGTCACGTCGCCATCGACGGCGCGCTGTTCCAGGATGAGGTCGAGGCGCGCCAGATCATG 
TCGGTGCCGAGCATCTACCTCAACGGCGAAGTCTTCGGCCAGGGCCGCATGGGCGTGGAAGAGATCCTCGCC 
AAGATCGACACCGGCGCCGCCGCCCGCGACGCCGAGAAGCTGACCGCCCGCGACGCCTTCGACGTACTGGTG 
GTCGGCGGTGGTCCGGCCGGCGCCGCGGCGGCGATCTACGCCGCGCGCAAGGGCATTCGCACCGGCGTCGCC 
GCCGAGCGCTTCGGCGGCCAGGTGCTGGACACCATGGCCATCGAGAACTTCATCTCGGTGCAGGAGACCGAC 
GGGCCGAAGCTGGCGCGGGCGCTGGAAGAGCACGTGCGTCACTACGAGGTCGACATCATGAACCTGCAGCGC 
GCCAGCAAGCTGGTTCCGGCGAAGAACACCGGCGAGTTGCACGAGGTGCAGTTCGAGAGCGGCGGCAGCCTC 
AAGGCCAAGACCCTGATCCTCGCCACCGGCGCTCGCTGGCGCGAAATGGGCGTGCCCGGCGAGCAGGAATAC 
AAGGCCAAGGGCGTGTGCTTCTGCCCGCACTGCGACGGCCCGCTGTTCAAGGGCAAGCGCGTGGCGGTGATC 
GGCGGCGGCAACTCTGGCGTCGAGGCGGCCATCGACCTGGCCGGCATCGTCGCCCATGTGACCCTGCTGGAA 
TTCGACAGCAAGTTGCGCGCCGATGCCGTGCTGCAACGCAAGCTCTACAGCCTGCCGAACGTCGAGGTGATC 
ACCAGTGCCCTGACCAGCGAAGTGAAGGGCGACGGCCAGAAGGTCACCGGCCTGGTGTACAAGGACCGCAAC 
TCCGAGGAGTTCAAGTCGATCGAGCTGGAAGGCATCTTCGTGCAGATCGGCCTGCTGCCCAACACCGAGTGG 
CTCAAGGGCAGCGTCGAGCTGTCGCCGCGTGGCGAGATCATCGTCGACGCCCGTGGCGAGACCTCGCTGCCC 
GGCATCTTCGCCGCCGGCGACGTGACCACGGTGCCGTACAAGCAGATCGTGATCGCCGTGGGCGAGGGCGCC 
AAGGCTTCGCTGAGCGCCTTCGACCACCTGATCCGGACCTCCGCGCCGGAGTGA