ViewGene
Locus Name:
GeneID:
Location:

GeneID:733
TrivialName: PA14_56300
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56300
PAO1 Ortholog Locus:PA4333
Sequence Length:1524
Protein Length:507
Start: 5025202
Stop: 5026725
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07721.1|AE004849_8 (AE004849) probable fumarase [Pseudomonas aeruginosa PAO1]
Identities = 507/507 (100%), Positives = 507/507 (100%)
Sequence:
ATGGCCGTGATCAAGCAAGACGACCTGATCCAGAGCGTCGCCGATGCCCTGCAGTTCATCTCCTACTACCAC
CCGGTCGATTTCATCCAGGCCATGCATGAGGCCTACCTGCGCGAAGAATCGCCGGCTGCCCGCGACTCCATG
GCGCAGATCCTGATCAACTCGCGCATGTGCGCCACCGGCCACCGCCCGATCTGCCAGGACACCGGGATCGTC
ACCGTGTTCGTCCGGGTCGGCATGGACGTGCGCTGGGACGGCGCTACCATGAGCGTCGACGATATGATCAAC
GAAGGCGTGCGCCGCGCCTACAACCTGCCGGAGAACGTCCTGCGCGCCTCGATCCTGGCCGACCCGGCCGGC
GCGCGGAAGAACACCAAGGACAACACGCCGGCGGTGATCCACTACTCCATCGTTCCCGGCGACAAGGTCGAA
GTCGATGTCGCGGCCAAGGGCGGCGGCTCGGAGAACAAGTCGAAGATGGCCATGCTCAACCCGTCCGACTCG
ATCGTCGACTGGGTGCTGAAGACTGTACCGACCATGGGCGCCGGCTGGTGCCCGCCGGGCATGCTCGGCATC
GGCATCGGCGGCACCGCGGAGAAAGCAGCGGTGATGGCGAAGGAAGTCCTCATGGACCCGATCGACATCCAC
GAACTGAAGGCCCGCGGCCCGCAGAACCGCATCGAGGAACTGCGCCTGGAGCTGTTCGAGAAGGTCAACCAG
CTGGGCATCGGTGCCCAGGGCCTGGGCGGCCTGACCACCGTGCTCGACGTCAAGATCATGGACTACCCGACC
CACGCCGCCTCGCTGCCGGTGTGCATGATCCCCAACTGCGCCGCGACCCGCCACGCCCACTTCGTGCTCGAC
GGCTCCGGCCCCGCCGAACTGGAAGCGCCGTCGCTCGACGCCTACCCGGAGATCGTCTGGGAAGCCGGCCCG
TCCGCGCGCCGCGTCGACCTGGACAAGATCACCCCGGAAGAGGTGCAAAGCTGGAAACCGGGCGAGACCCTG
CTGCTCAACGGCAAGATGCTCACCGGCCGCGACGCCGCGCACAAGCGCATGGTCGACATGCTGAACAAGGGC
GAAACGCTGCCGGTGGACCTGAAAGGCCGCTTCATCTATTACGTCGGCCCGGTCGATCCGGTCGGCGACGAG
GTGGTCGGCCCGGCCGGTCCCACCACCGCCACCCGGATGGACAAGTTCACCCGCCAGATCCTCGAACAGACC
GGCCTGCTGGGCATGATCGGCAAGTCCGAGCGCGGTCCCATCGCCATCGAGGCGATCAAGGACAACAAGGCG
GTATACCTGATGGCCGTCGGCGGCGCCGCCTACCTGGTCGCCCAGGCGATCAAGAAGTCCAAGGTGCTGGCC
TTCGCCGAACTGGGCATGGAAGCGATCTACGAGTTCGAGGTGAAGGACATGCCGGTCACCGTCGCGGTCGAT
ACCAACGGCGAGTCGGTGCACATCACCGGCCCGGCGGTCTGGCAGAAGAAGATCGCCGAAAGCCTGGCGGTC
GAGGTCAAGTAA
Translation:
MAVIKQDDLIQSVADALQFISYYHPVDFIQAMHEAYLREESPAARDSMAQILINSRMCATGHRPICQDTGIV
TVFVRVGMDVRWDGATMSVDDMINEGVRRAYNLPENVLRASILADPAGARKNTKDNTPAVIHYSIVPGDKVE
VDVAAKGGGSENKSKMAMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAAVMAKEVLMDPIDIH
ELKARGPQNRIEELRLELFEKVNQLGIGAQGLGGLTTVLDVKIMDYPTHAASLPVCMIPNCAATRHAHFVLD
GSGPAELEAPSLDAYPEIVWEAGPSARRVDLDKITPEEVQSWKPGETLLLNGKMLTGRDAAHKRMVDMLNKG
ETLPVDLKGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQILEQTGLLGMIGKSERGPIAIEAIKDNKA
VYLMAVGGAAYLVAQAIKKSKVLAFAELGMEAIYEFEVKDMPVTVAVDTNGESVHITGPAVWQKKIAESLAV
EVK*
AnnotationID:742GeneID:733
AnnotatorUID: diggins
Modification Date/Time: 2005-04-04 16:04:19
Gene Name:
Confidence Code:3
GeneProduct:putative fumarase
Cell Localization:()
Synonyms:fumA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:putative fumarate hydratase
Functional Category Confidence Code:5
COGs:pfam05681,pfam05683,COG1838
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66522.1|AE016281_3 (AE016777) fumarate hydratase, class I [Pseudomonas putida KT2440]
          Length = 507

 Score =  978 bits (2527), Expect = 0.0
 Identities = 486/507 (95%), Positives = 500/507 (98%)

>gb|AAO57790.1| (AE016871) fumarate hydratase, class I, putative [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 507

 Score =  958 bits (2477), Expect = 0.0
 Identities = 475/507 (93%), Positives = 497/507 (98%)

>gb|AAM36330.1| (AE011778) fumarate hydratase [Xanthomonas axonopodis pv. citri
           str. 306]
          Length = 505

 Score =  794 bits (2050), Expect = 0.0
 Identities = 389/500 (77%), Positives = 445/500 (89%), Gaps = 1/500 (0%)








 
Structural Features:
pfam05681, Fumerase, Fumarate hydratase (Fumerase). 
CD-Length = 271 residues, 100.0% aligned
Score =  354 bits (909), Expect = 2e-98
Identities = 150/282 (53%), Positives = 186/282 (65%), Gaps = 14/282 (4%)

pfam05683, Fumerase_C, Fumarase C-terminus. 
CD-Length = 209 residues,  99.5% aligned
Score =  305 bits (782), Expect = 1e-83
Identities = 133/208 (63%), Positives = 153/208 (73%), Gaps = 4/208 (1%)

COG1838, FumA, Tartrate dehydratase beta subunit/Fumarate hydratase class I, 
C-terminal domain 
[Energy production and conversion]
CD-Length = 184 residues, 100.0% aligned
Score =  250 bits (639), Expect = 4e-67
Identities = 102/186 (54%), Positives = 135/186 (72%), Gaps = 2/186 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1469




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18611




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36688




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:733_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:733
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1279
                           PA4333_tr
                           translation of PA4333
                           507
                           99.99
                           99.99
                           0
                           2610.0
     

     
     
48338
                           gb|AAG07721.1|AE004849_8
                           (AE004849) probable fumarase [Pseudomonas aeruginosa PAO1]
                           507
                           99.99
                           99.99
                           0
                           2610.0
     

     
     
48339
                           gb|AAN66522.1|AE016281_3
                           (AE016777) fumarate hydratase, class I [Pseudomonas putida KT2440]
                           507
                           95.85
                           98.61
                           0
                           2527.0
     

     
     
48340
                           gb|AAO57790.1|
                           (AE016871) fumarate hydratase, class I, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           507
                           93.68
                           98.02
                           0
                           2477.0
     

     
     
48341
                           gb|AAM36330.1|
                           (AE011778) fumarate hydratase [Xanthomonas axonopodis pv. citri str. 306]
                           508
                           76.96
                           88.18
                           4
                           2051.0
     

     






      

          
GC ORFID: 43735How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4333Glimmer Score: 

          
Start: 4288878Stop: 4290401

          
Length: 1524


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4333 translation of PA4333Homolog Bit Score 1009.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene1953 (ORF: BLASTX 962.0), t_PA4333 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCGTGATCAAGCAAGACGACCTGATCCAGAGCGTCGCCGATGCCCTGCAGTTCATCTCCTACTACCAC 
CCGGTCGATTTCATCCAGGCCATGCATGAGGCCTACCTGCGCGAAGAATCGCCGGCTGCCCGCGACTCCATG 
GCGCAGATCCTGATCAACTCGCGCATGTGCGCCACCGGCCACCGCCCGATCTGCCAGGACACCGGGATCGTC 
ACCGTGTTCGTCCGGGTCGGCATGGACGTGCGCTGGGACGGCGCTACCATGAGCGTCGACGATATGATCAAC 
GAAGGCGTGCGCCGCGCCTACAACCTGCCGGAGAACGTCCTGCGCGCCTCGATCCTGGCCGACCCGGCCGGC 
GCGCGGAAGAACACCAAGGACAACACGCCGGCGGTGATCCACTACTCCATCGTTCCCGGCGACAAGGTCGAA 
GTCGATGTCGCGGCCAAGGGCGGCGGCTCGGAGAACAAGTCGAAGATGGCCATGCTCAACCCGTCCGACTCG 
ATCGTCGACTGGGTGCTGAAGACTGTACCGACCATGGGCGCCGGCTGGTGCCCGCCGGGCATGCTCGGCATC 
GGCATCGGCGGCACCGCGGAGAAAGCAGCGGTGATGGCGAAGGAAGTCCTCATGGACCCGATCGACATCCAC 
GAACTGAAGGCCCGCGGCCCGCAGAACCGCATCGAGGAACTGCGCCTGGAGCTGTTCGAGAAGGTCAACCAG 
CTGGGCATCGGTGCCCAGGGCCTGGGCGGCCTGACCACCGTGCTCGACGTCAAGATCATGGACTACCCGACC 
CACGCCGCCTCGCTGCCGGTGTGCATGATCCCCAACTGCGCCGCGACCCGCCACGCCCACTTCGTGCTCGAC 
GGCTCCGGCCCCGCCGAACTGGAAGCGCCGTCGCTCGACGCCTACCCGGAGATCGTCTGGGAAGCCGGCCCG 
TCCGCGCGCCGCGTCGACCTGGACAAGATCACCCCGGAAGAGGTGCAAAGCTGGAAACCGGGCGAGACCCTG 
CTGCTCAACGGCAAGATGCTCACCGGCCGCGACGCCGCGCACAAGCGCATGGTCGACATGCTGAACAAGGGC 
GAAACGCTGCCGGTGGACCTGAAAGGCCGCTTCATCTATTACGTCGGCCCGGTCGATCCGGTCGGCGACGAG 
GTGGTCGGCCCGGCCGGTCCCACCACCGCCACCCGGATGGACAAGTTCACCCGCCAGATCCTCGAACAGACC 
GGCCTGCTGGGCATGATCGGCAAGTCCGAGCGCGGTCCCATCGCCATCGAGGCGATCAAGGACAACAAGGCG 
GTATACCTGATGGCCGTCGGCGGCGCCGCCTACCTGGTCGCCCAGGCGATCAAGAAGTCCAAGGTGCTGGCC 
TTCGCCGAACTGGGCATGGAAGCGATCTACGAGTTCGAGGTGAAGGACATGCCGGTCACCGTCGCGGTCGAT 
ACCAACGGCGAGTCGGTGCACATCACCGGCCCGGCGGTCTGGCAGAAGAAGATCGCCGAAAGCCTGGCGGTC 
GAGGTCAAGTAA