ViewGene
Locus Name:
GeneID:
Location:

GeneID:732
TrivialName: PA14_29860
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29860
PAO1 Ortholog Locus:PA2648
Sequence Length:1530
Protein Length:509
Start: 2587177
Stop: 2585648
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06036.1|AE004693_7 (AE004693) NADH dehydrogenase I chain M [Pseudomonas aeruginosa PAO1]
Identities = 508/509 (99%), Positives = 509/509 (100%)
Sequence:
ATGATTCTGCCCTGGCTAATCCTGATCCCCTTCATCGGCGGTTTCCTCTGCTGGATCGCCGAACACAGCAGC
AAGACCCTACCCCGCTGGATCGCGCTGCTTTCGATGACCCTGGTCCTGATCCTGAGCCTGTGGATCTGGGCC
ACCGGCGACTTCCAGCTGGCGCCCGCGCCCGGCGGCGAGCCGGAATGGACCCTGCAATTCAAGGTGCTGTGG
ATCGAGCGTCTCGGCATCAGCGTCCACCTGGCGATGGATGGCCTGTCGCTGCTGATGGTCGCCCTCACCGGC
CTGCTCGGCGTGCTCTCGGTCCTCTGCTCCTGGAACGAGATCCAGCGCCGCATCGGCTTCTTCCATCTGAAC
CTGCTGTGGATCCTGGGCGGTGTGATCGGCGTGTTCCTCGCCATCGACCTGTTCCTGTTCTTCTTCTTCTGG
GAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTGTGGGGACACAGCTCCGATGACGGCAAGAAGACC
CGCATCTACGCCGCCACCAAGTTCTTCATCTTCACCCAGGCCAGCGGCCTGGTGATGCTGGTGGCGATCCTC
GGCCTGGTGTTCGTCAACTTCAATGCCACCGGCGTGATCACCTTCGACTACGCCACCCTGCTGAAGACCCAG
CTAAGCCCCCACGTGGAATGGCTGCTGATGCTCGGCTTCTTCGTCGCCTTCGCGGTGAAGATGCCAGTGGTG
CCGGTGCATTCCTGGCTGCCGGACGCCCACGCCCAGGCGCCCACCGCCGGCTCCGTGGACCTCGCCGGCATC
CTCCTGAAGACCGCCGCCTACGGCCTGATCCGCTTCGCCCTGCCGCTGTTCCCCAACGCCTCGGCGGAATTC
GCGCCGATCGCCATGTGGCTCGGCATCATCGGCATCTTCTACGGTGCCCTGCTGTCCTTCGCGCAGACCGAC
ATCAAGCGCCTGGTCGCCTACTCCAGCGTTTCGCACATGGGCTTCGTGATGATCGGCATCTACTCCGGCAGC
CAGGTCGCGCTGCAGGGCGTGGTGGTACAGATGATCGCCCACGGCCTCTCCGCCGCGGCGCTGTTCATCCTC
TGCGGCCAGCTCTACGAGCGCCTGCATACCCGCGACATGCGCAAGATGGGCGGCCTGTGGTCGCGCATGCCC
TACCTGCCGGCGATCAGCCTGTTCTTCGCCTCGGCGTCGCTGGGCCTGCCCGGCACCGGCAACTTCGTCGGC
GAGTTCCTGATCCTGATCGGCGCCTTCAAGGTGGTCCCGGTGATCATCGTGATCGCCACCTTCGGCCTGGTC
TTCGCCTCGGTCTACTCGCTGATCATGATCCACCGCGCCTACTTCGGACCCAGCCAGTCCGACGAGCCGATC
CTCGGCCTCGACGCCCGCGAACTGAGCATGGTGCTCGGCCTGGCGGTCCTGCTGGTCCTGCTCGGGGTCTAC
CCGCAGCCGGTGCTGGACATCTCCGCGGCCAGCATGCACGGCGTGCAGCAATGGCTGGGCGCAGCCCTTTCC
ACCCTTGCAGGACGGTAG
Translation:
MILPWLILIPFIGGFLCWIAEHSSKTLPRWIALLSMTLVLILSLWIWATGDFQLAPAPGGEPEWTLQFKVLW
IERLGISVHLAMDGLSLLMVALTGLLGVLSVLCSWNEIQRRIGFFHLNLLWILGGVIGVFLAIDLFLFFFFW
EMMLVPMYFLIALWGHSSDDGKKTRIYAATKFFIFTQASGLVMLVAILGLVFVNFNATGVITFDYATLLKTQ
LSPHVEWLLMLGFFVAFAVKMPVVPVHSWLPDAHAQAPTAGSVDLAGILLKTAAYGLIRFALPLFPNASAEF
APIAMWLGIIGIFYGALLSFAQTDIKRLVAYSSVSHMGFVMIGIYSGSQVALQGVVVQMIAHGLSAAALFIL
CGQLYERLHTRDMRKMGGLWSRMPYLPAISLFFASASLGLPGTGNFVGEFLILIGAFKVVPVIIVIATFGLV
FASVYSLIMIHRAYFGPSQSDEPILGLDARELSMVLGLAVLLVLLGVYPQPVLDISAASMHGVQQWLGAALS
TLAGR*
AnnotationID:741GeneID:732
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 12:12:09
Gene Name:nuoM
Confidence Code:2
GeneProduct:NADH dehydrogenase I chain M
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1008
Secondary Functional Category(ies):
EC Number:1.6.5.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL89575.1| (AF281148) NADH dehydrogenase I subunit M [Pseudomonas fluorescens]
          Length = 510

 Score =  844 bits (2180), Expect = 0.0
 Identities = 415/507 (81%), Positives = 453/507 (89%)

>gb|AAN69713.1|AE016607_7 (AE016789) NADH dehydrogenase I, M subunit [Pseudomonas putida
           KT2440]
          Length = 510

 Score =  841 bits (2172), Expect = 0.0
 Identities = 409/507 (80%), Positives = 458/507 (90%)

>gb|AAM85210.1|AE013767_7 (AE013767) NADH dehydrogenase I chain M [Yersinia pestis KIM]
          Length = 510

 Score =  769 bits (1985), Expect = 0.0
 Identities = 376/502 (74%), Positives = 434/502 (86%), Gaps = 2/502 (0%)








 
Structural Features:
COG1008, NuoM, NADH:ubiquinone oxidoreductase subunit 4 (chain M) 
[Energy production and conversion]
CD-Length = 497 residues,  98.8% aligned
Score =  518 bits (1337), Expect = 5e-148
Identities = 255/509 (50%), Positives = 339/509 (66%), Gaps = 20/509 (3%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1467




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18609




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33198




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:732_cdd.html


    		




 
URL:

















    ReferenceID:34583




  
Author/Investigator(s):
        Camacho Carvajal MM, Wijfjes AH, Mulders IH, Lugtenberg BJ, Bloemberg GV.





  
Title:
        Characterization of NADH dehydrogenases of Pseudomonas fluorescens WCS365 and their role in competitive root colonization.




  
PubMed:
        12118882


MedLine:
        




    
Source:
Mol Plant Microbe Interact. 2002 Jul;15(7):662-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12118882

















    ReferenceID:34593




  
Author/Investigator(s):
        Dupuis A, Peinnequin A, Chevallet M, Lunardi J, Darrouzet E, Pierrard B, Procaccio V, Issartel JP.





  
Title:
        Identification of five Rhodobacter capsulatus genes encoding the equivalent of ND subunits of the mitochondrial NADH-ubiquinone oxidoreductase.




  
PubMed:
        8566820


MedLine:
        




    
Source:
Gene. 1995 Dec 29;167(1-2):99-104.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8566820









           
Homologs By Global Alignment 

           

       



     
Gene ID:732
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1278
                           PA2648_tr
                           translation of PA2648
                           509
                           99.80
                           99.99
                           0
                           2610.0
     

     
     
48308
                           gb|AAG06036.1|AE004693_7
                           (AE004693) NADH dehydrogenase I chain M [Pseudomonas aeruginosa PAO1]
                           509
                           99.80
                           99.99
                           0
                           2610.0
     

     
     
48309
                           gb|AAL89575.1|
                           (AF281148) NADH dehydrogenase I subunit M [Pseudomonas fluorescens]
                           510
                           81.37
                           88.82
                           1
                           2179.0
     

     
     
48310
                           gb|AAN69713.1|AE016607_7
                           (AE016789) NADH dehydrogenase I, M subunit [Pseudomonas putida KT2440]
                           510
                           80.19
                           89.80
                           1
                           2171.0
     

     
     
48311
                           gb|AAO56854.1|
                           (AE016868) NADH dehydrogenase I, M subunit [Pseudomonas syringae pv. tomato str. DC3000]
                           510
                           80.19
                           89.21
                           1
                           2167.0
     

     






      

          
GC ORFID: 43734How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2648Glimmer Score: 

          
Start: 2366749Stop: 2368278

          
Length: 1530


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2648 translation of PA2648Homolog Bit Score 1009.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2776 (ORF: BLASTX 843.0), t_PA2648 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATTCTGCCCTGGCTAATCCTGATCCCCTTCATCGGCGGTTTCCTCTGCTGGATCGCCGAACACAGCAGC 
AAGACCCTACCCCGCTGGATCGCGCTGCTTTCGATGACCCTGGTCCTGATCCTGAGCCTGTGGATCTGGGCC 
ACCGGCGACTTCCAGCTGGCGCCCGCGCCCGGCGGCGAGCCGGAATGGACCCTGCAATTCAAGGTGCTGTGG 
ATCGAGCGTCTCGGCATCAGCGTCCACCTGGCGATGGATGGCCTGTCGCTGCTGATGGTCGCCCTCACCGGC 
CTGCTCGGCGTGCTCTCGGTCCTCTGCTCCTGGAACGAGATCCAGCGCCGCATCGGCTTCTTCCATCTGAAC 
CTGCTGTGGATCCTGGGCGGTGTGATCGGCGTGTTCCTCGCCATCGACCTGTTCCTGTTCTTCTTCTTCTGG 
GAAATGATGCTGGTGCCGATGTACTTCCTCATCGCGCTGTGGGGACACAGCTCCGATGACGGCAAGAAGACC 
CGCATCTACGCCGCCACCAAGTTCTTCATCTTCACCCAGGCCAGCGGCCTGGTGATGCTGGTGGCGATCCTC 
GGCCTGGTGTTCGTCAACTTCAATGCCACCGGCGTGATCACCTTCGACTACGCCACCCTGCTGAAGACCCAG 
CTAAGCCCCCACGTGGAATGGCTGCTGATGCTCGGCTTCTTCGTCGCCTTCGCGGTGAAGATGCCAGTGGTG 
CCGGTGCATTCCTGGCTGCCGGACGCCCACGCCCAGGCGCCCACCGCCGGCTCCGTGGACCTCGCCGGCATC 
CTCCTGAAGACCGCCGCCTACGGCCTGATCCGCTTCGCCCTGCCGCTGTTCCCCAACGCCTCGGCGGAATTC 
GCGCCGATCGCCATGTGGCTCGGCATCATCGGCATCTTCTACGGTGCCCTGCTGTCCTTCGCGCAGACCGAC 
ATCAAGCGCCTGGTCGCCTACTCCAGCGTTTCGCACATGGGCTTCGTGATGATCGGCATCTACTCCGGCAGC 
CAGGTCGCGCTGCAGGGCGTGGTGGTACAGATGATCGCCCACGGCCTCTCCGCCGCGGCGCTGTTCATCCTC 
TGCGGCCAGCTCTACGAGCGCCTGCATACCCGCGACATGCGCAAGATGGGCGGCCTGTGGTCGCGCATGCCC 
TACCTGCCGGCGATCAGCCTGTTCTTCGCCTCGGCGTCGCTGGGCCTGCCCGGCACCGGCAACTTCGTCGGC 
GAGTTCCTGATCCTGATCGGCGCCTTCAAGGTGGTCCCGGTGATCATCGTGATCGCCACCTTCGGCCTGGTC 
TTCGCCTCGGTCTACTCGCTGATCATGATCCACCGCGCCTACTTCGGACCCAGCCAGTCCGACGAGCCGATC 
CTCGGCCTCGACGCCCGCGAACTGAGCATGGTGCTCGGCCTGGCGGTCCTGCTGGTCCTGCTCGGGGTCTAC 
CCGCAGCCGGTGCTGGACATCTCCGCGGCCAGCATGCACGGCGTGCAGCAATGGCTGGGCGCAGCCCTTTCC 
ACCCTTGCAGGACGGTAG