ViewGene
Locus Name:
GeneID:
Location:

GeneID:728
TrivialName: PA14_61400
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_61400
PAO1 Ortholog Locus:PA4640
Sequence Length:1524
Protein Length:507
Start: 5480421
Stop: 5481944
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08028.1|AE004878_6 (AE004878) malate:quinone oxidoreductase [Pseudomonas aeruginosa PAO1]
Identities = 506/507 (99%), Positives = 507/507 (100%)
Sequence:
ATGGCGCAAAACGATCACGAAACAGTCGACATGCTGCTGGTAGGCGCCGGCATCATGAGCGCGACCCTGGCG
GTCCTGCTCAAGGAACTCGACCCCAACCTGAAGATGGAAGTCGTCGAACTCCAGGAGTCCGGCGCGATCGAA
AGCTCCAACCCGTGGAACAACGCGGGCACCGGCCACGCCGGCCTGTGCGAGCTGAACTACACCCCGCAGTCG
GCCGACGGCAGCATCGACATCAAGAAGGCGGTGGGCATCAACACCATGTTCGAGGTGTCGAAGCAATTCTGG
TCGCACCTGGTCGCCAAGGGCACCTTCGGCTCGCCGAAGACCTTCATCAACCCGGTGCCGCACCTGAGCTTC
GTGCGCGGCAGCGAAGGCATCGCCTACCTGAAGAAGCGCTTCGAATCACTGACCAGGCACCACGCCTTCGAG
ACCATGGTCTACTCCGAGGACAAGGCCACCCTCGCCGAGTGGATGCCGCTGATGATGCCGGGTCGTCCGGCC
GACGAAGCGATCGCCGCGACCCGCGTCGAGGGCGGTACCGACGTCAACTTCGGCGCCCTGACCAACCAGCTC
CTGCAACACCTGGCGCAACAGCCGGGCGCGCAGATCCGCTACAACCAGAAGGTCACCCACCTGCGCCGCGCC
GATAACGGCTGGCGGGTCACCGTCAAGGACACCCGCAACGGCGGCGACCGCGAGATCCAGGCACGCTTCGTC
TTCCTCGGCGCCGGCGGCGGCGCCCTGCCGCTCCTGCAACTTTCCGGCATCCCGGAAGGCAAGGGCTTCGGT
GGTTTCCCGGTGAGCGGCCAGTGGCTGCGCTGCGACAACCCGGAAATCGTCAAGCAGCACCAGGCCAAGGTC
TACAGCCAGGCCGAGGTCGGCTCGCCGCCGATGTCCGTGCCGCACCTGGACACCCGCGTGGTGGACGGCAAG
AAATCCCTGCTGTTCGGCCCCTATGCCGGTTTCTCCACCAAGTTCCTGCGCCATGGCTCGTTCCTCGACCTG
CCGCTGTCGGTACGCCCGGGCAACATCCTGCCGATGCTCTCGGTGGCCCGCGACAACATGGACCTGACCCGC
TACCTGATCGGCCAGGTCATGCAATCGCCGGAACAGCGCCTGGAAGCCCTGCGCAAGTTCTACCCCGAAGCC
AGGGCCGAGGACTGGCGCCTGGAAGTCGCCGGCCAGCGCGTGCAGATCATCAAGAAGGATCCAAAGAAAGGC
GGCATCCTGCAGTTCGGCACCGAGCTGGTGGCCGCCCACGACGGCTCCATCGCCGCCCTGCTGGGCGCCTCG
CCGGGCGCCTCGGTGACCGTCTCGATCATGCTCGGCCTGATCGAGCGCTGCTTCCCGGAGCAGGCCCGCTCG
CCGGAGTGGAGCGCCAAGCTGAAGGAAATCTTCCCGGCTCGCGAGAAAGAGCTGGAAAGCGACGCCGAGCTT
TACCGCAGCGTCAGCAGCCGTTGCAGCGAGGTGCTGGAACTGACCGCGAAGAACGACGTGCAGGCCCCGGTC
AACGCCGAGTGA
Translation:
MAQNDHETVDMLLVGAGIMSATLAVLLKELDPNLKMEVVELQESGAIESSNPWNNAGTGHAGLCELNYTPQS
ADGSIDIKKAVGINTMFEVSKQFWSHLVAKGTFGSPKTFINPVPHLSFVRGSEGIAYLKKRFESLTRHHAFE
TMVYSEDKATLAEWMPLMMPGRPADEAIAATRVEGGTDVNFGALTNQLLQHLAQQPGAQIRYNQKVTHLRRA
DNGWRVTVKDTRNGGDREIQARFVFLGAGGGALPLLQLSGIPEGKGFGGFPVSGQWLRCDNPEIVKQHQAKV
YSQAEVGSPPMSVPHLDTRVVDGKKSLLFGPYAGFSTKFLRHGSFLDLPLSVRPGNILPMLSVARDNMDLTR
YLIGQVMQSPEQRLEALRKFYPEARAEDWRLEVAGQRVQIIKKDPKKGGILQFGTELVAAHDGSIAALLGAS
PGASVTVSIMLGLIERCFPEQARSPEWSAKLKEIFPAREKELESDAELYRSVSSRCSEVLELTAKNDVQAPV
NAE*
AnnotationID:737GeneID:728
AnnotatorUID: danlee
Modification Date/Time: 2006-01-30 13:01:59
Gene Name:mqoB
Confidence Code:1
GeneProduct: malate:quinone oxidoreductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:malate dehydrogenase
Functional Category Confidence Code:5
COGs:pfam06039,COG0579
Secondary Functional Category(ies):Energy metabolism
EC Number:1.1.99.16
Status:ACTIVE
Pathway:
Citrate cycle (TCA cycle); Pyruvate metabolism
Homology:
>gb|AAO54665.1| (AE016859) malate:quinone oxidoreductase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 502

 Score =  773 bits (1996), Expect = 0.0
 Identities = 386/497 (77%), Positives = 429/497 (86%), Gaps = 3/497 (0%)

>gb|AAD51981.1|AF176206_1 (AF176206) malate:quinone oxidoreductase [Pseudomonas fluorescens]
          Length = 505

 Score =  736 bits (1901), Expect = 0.0
 Identities = 363/499 (72%), Positives = 423/499 (84%), Gaps = 2/499 (0%)
Structural Features:
pfam06039, Mqo, Malate:quinone oxidoreductase (Mqo).
CD-Length = 489 residues, 100.0% aligned
Score =  831 bits (2148), Expect = 0.0
Identities = 311/491 (63%), Positives = 387/491 (78%), Gaps = 3/491 (0%)

COG0579, Predicted dehydrogenase 
CD-Length = 429 residues, 100.0% aligned
Score =  394 bits (1013), Expect = 3e-110
Identities = 180/476 (37%), Positives = 241/476 (50%), Gaps = 50/476 (10%)
Genomic Context:






   
Comment:


















    ReferenceID:1459




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18601




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31585




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:728_cdd.html


    		




 
URL:

















    ReferenceID:33329




  
Author/Investigator(s):
        Gorisch H.





  
Title:
        The ethanol oxidation system and its regulation in Pseudomonas aeruginosa.




  
PubMed:
        12686116


MedLine:
        




    
Source:
Biochim Biophys Acta. 2003 Apr 11;1647(1-2):98-102.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12686116

















    ReferenceID:33330




  
Author/Investigator(s):
        Kretzschmar U, Ruckert A, Jeoung JH, Gorisch H.





  
Title:
        Malate:quinone oxidoreductase is essential for growth on ethanol or acetate in Pseudomonas aeruginosa.




  
PubMed:
        12480887


MedLine:
        




    
Source:
Microbiology. 2002 Dec;148(Pt 12):3839-47.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12480887









           
Homologs By Global Alignment 

           

       



     
Gene ID:728
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1260
                           PA4640_tr
                           translation of PA4640
                           507
                           99.80
                           99.99
                           0
                           2617.0
     

     
     
47961
                           gb|AAG08028.1|AE004878_6
                           (AE004878) malate:quinone oxidoreductase [Pseudomonas aeruginosa PAO1]
                           507
                           99.80
                           99.99
                           0
                           2617.0
     

     
     
47962
                           gb|AAN01356.1|
                           (AY129296) malate:quinone oxidoreductase [Pseudomonas aeruginosa]
                           507
                           99.80
                           99.99
                           0
                           2617.0
     

     
     
47963
                           gb|AAO54665.1|
                           (AE016859) malate:quinone oxidoreductase [Pseudomonas syringae pv. tomato str. DC3000]
                           507
                           76.13
                           84.81
                           5
                           1995.0
     

     
     
47964
                           gb|AAN66376.1|AE016265_10
                           (AE016776) malate:quinone oxidoreductase [Pseudomonas putida KT2440]
                           512
                           74.60
                           84.76
                           15
                           1988.0
     

     






      

          
GC ORFID: 43730How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4640Glimmer Score: 

          
Start: 4744101Stop: 4745624

          
Length: 1524


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4640 translation of PA4640Homolog Bit Score 1012.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene5069 (ORF: BLASTX 774.0), t_PA3452 (ORF: BLASTX 546.0), psyr_15may02_Scaffold7_revised_gene6969 (ORF: BLASTX 492.0), t_PA4640 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGCAAAACGATCACGAAACAGTCGACATGCTGCTGGTAGGCGCCGGCATCATGAGCGCGACCCTGGCG 
GTCCTGCTCAAGGAACTCGACCCCAACCTGAAGATGGAAGTCGTCGAACTCCAGGAGTCCGGCGCGATCGAA 
AGCTCCAACCCGTGGAACAACGCGGGCACCGGCCACGCCGGCCTGTGCGAGCTGAACTACACCCCGCAGTCG 
GCCGACGGCAGCATCGACATCAAGAAGGCGGTGGGCATCAACACCATGTTCGAGGTGTCGAAGCAATTCTGG 
TCGCACCTGGTCGCCAAGGGCACCTTCGGCTCGCCGAAGACCTTCATCAACCCGGTGCCGCACCTGAGCTTC 
GTGCGCGGCAGCGAAGGCATCGCCTACCTGAAGAAGCGCTTCGAATCACTGACCAGGCACCACGCCTTCGAG 
ACCATGGTCTACTCCGAGGACAAGGCCACCCTCGCCGAGTGGATGCCGCTGATGATGCCGGGTCGTCCGGCC 
GACGAAGCGATCGCCGCGACCCGCGTCGAGGGCGGTACCGACGTCAACTTCGGCGCCCTGACCAACCAGCTC 
CTGCAACACCTGGCGCAACAGCCGGGCGCGCAGATCCGCTACAACCAGAAGGTCACCCACCTGCGCCGCGCC 
GATAACGGCTGGCGGGTCACCGTCAAGGACACCCGCAACGGCGGCGACCGCGAGATCCAGGCACGCTTCGTC 
TTCCTCGGCGCCGGCGGCGGCGCCCTGCCGCTCCTGCAACTTTCCGGCATCCCGGAAGGCAAGGGCTTCGGT 
GGTTTCCCGGTGAGCGGCCAGTGGCTGCGCTGCGACAACCCGGAAATCGTCAAGCAGCACCAGGCCAAGGTC 
TACAGCCAGGCCGAGGTCGGCTCGCCGCCGATGTCCGTGCCGCACCTGGACACCCGCGTGGTGGACGGCAAG 
AAATCCCTGCTGTTCGGCCCCTATGCCGGTTTCTCCACCAAGTTCCTGCGCCATGGCTCGTTCCTCGACCTG 
CCGCTGTCGGTACGCCCGGGCAACATCCTGCCGATGCTCTCGGTGGCCCGCGACAACATGGACCTGACCCGC 
TACCTGATCGGCCAGGTCATGCAATCGCCGGAACAGCGCCTGGAAGCCCTGCGCAAGTTCTACCCCGAAGCC 
AGGGCCGAGGACTGGCGCCTGGAAGTCGCCGGCCAGCGCGTGCAGATCATCAAGAAGGATCCAAAGAAAGGC 
GGCATCCTGCAGTTCGGCACCGAGCTGGTGGCCGCCCACGACGGCTCCATCGCCGCCCTGCTGGGCGCCTCG 
CCGGGCGCCTCGGTGACCGTCTCGATCATGCTCGGCCTGATCGAGCGCTGCTTCCCGGAGCAGGCCCGCTCG 
CCGGAGTGGAGCGCCAAGCTGAAGGAAATCTTCCCGGCTCGCGAGAAAGAGCTGGAAAGCGACGCCGAGCTT 
TACCGCAGCGTCAGCAGCCGTTGCAGCGAGGTGCTGGAACTGACCGCGAAGAACGACGTGCAGGCCCCGGTC 
AACGCCGAGTGA