ViewGene
Locus Name:
GeneID:
Location:

GeneID:723
TrivialName: PA14_47100
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47100
PAO1 Ortholog Locus:PA1326
Sequence Length:1548
Protein Length:515
Start: 4197566
Stop: 4196019
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04715.1|AE004562_2 (AE004562) threonine dehydratase, biosynthetic [Pseudomonas aeruginosa PAO1]
Identities = 515/515 (100%), Positives = 515/515 (100%)
Sequence:
ATGAGCGCCTCGACCCTCAGCGCAAGCAAACCCCTGCTGGCCGGCTACGTGGAAAGCATCCTGGCCGCGCCG
GTGTACGACGTCGCCGTGGAAACCCCCTTGCAGGTCGCGCCGCAGTTGTCCCAGCGCCTGGGCAACCGGGTG
CTGCTCAAGCGCGAGGACCTGCAGCCGGTGTTCTCCTTCAAGATCCGCGGCGCCTATACCCGTGTCGCGCGC
CTGAGCGACGAGCAGAAGGCCCGCGGGGTGATCACCGCCTCCGCCGGCAACCATGCCCAGGGCCTGGCCCTG
GCGGCGCAGCGCCTGGGCGTGCGGGCGGTGATCGTGATGCCGCGCACCACTCCCGAGCTCAAGGTCAAGGGC
GTGCTGGCGCGCGGCGGCGAGGCGTTGCTGCACGGCGACGCTTTCCCCGATGCGCTGGCCCATGCCCTGCAA
CTGGCCGAGCGCGAAGGCATGACCTTCGTCCCGCCCTACGACGATCCGGACGTGATCGCCGGACAGGGCACG
GTGGCGATGGAGATCCTCCGCCAGCACAGCGGCCGCCTCGACGCGATCTTCGTGCCGGTCGGCGGCGGCAGC
CTGATTGCCGGGATCGCCGCCTACGTCAAGCACCTGCGTCCGGATATCCGCGTGATCGGCGTCGAGCCGGAG
GATTCCAACTGCCTGCAGGCGGCGCTGGCCGCCGGCGAACGGGTGGTGCTGGGCCAGGTCGGGCTGTTCGCC
GACGGGGTGGCGGTGGCGCAGATCGGCGCTTGCAACTTCGAGGTCTGCAAGGACCATGTCGACGAGGTGATC
ACCGTCGGCAGCGACGAAATCTGCGCGGCGATCAAGGACATCTACGACGACACCCGTTCGATCACCGAGCCG
GCCGGCGCCCTGGCCGTAGCGGGGATCAAGAAGTACGTGGCCCGCGAACGCACCGAGGGGCAGACCCTGGTG
GCCATCGACTCCGGTGCCAACATCAACTTCGACCGCCTGCGCCACGTTGCCGAGCGCGCCGAACTGGGCGAA
CAGCGCGAAGCGATCATCGCCGTCACCGTGGCCGAGCGGCCGGGCAGCTTCAAGGCCTTCTGCGCGGCGCTG
GGCCGGCGCCAGATCACCGAGTTCAACTACCGCTACCACAGCGACCGCCAGGCCCACCTGTTCGTCGGCGTG
CAGACCCATCCGCTCACCGACAGTCGCGCCGACCTGCTCGCCGGCCTGCGTGAGCAGGGCTTCCCGGTACTC
GACCTGACCGACAACGAGATGGCCAAGCTGCACATCCGCCACATGGTCGGCGGCCACGGTACCGAGGTGCGC
CGCGAGCGGCTGTTCCGCTTCGAGTTTCCCGAACGCCCCGGGGCCTTGCTGAACTTTCTCGACAAGCTCGGC
TCGCGCTGGAACATCAGCCTGTTCCACTACCGCAACCACGGCGCCGCCGACGGTCGGGTGCTGGCCGGATTG
CAGGTGCCGGACGAGGAGCGCGGCGAGCTGGAGGCGGCGTTGCAAGCCATCGGCTATCCGTACTGGGAGGAA
ACCCACAACCCGGCCTACCGGCTGTTCGCCGGTTGA
Translation:
MSASTLSASKPLLAGYVESILAAPVYDVAVETPLQVAPQLSQRLGNRVLLKREDLQPVFSFKIRGAYTRVAR
LSDEQKARGVITASAGNHAQGLALAAQRLGVRAVIVMPRTTPELKVKGVLARGGEALLHGDAFPDALAHALQ
LAEREGMTFVPPYDDPDVIAGQGTVAMEILRQHSGRLDAIFVPVGGGSLIAGIAAYVKHLRPDIRVIGVEPE
DSNCLQAALAAGERVVLGQVGLFADGVAVAQIGACNFEVCKDHVDEVITVGSDEICAAIKDIYDDTRSITEP
AGALAVAGIKKYVARERTEGQTLVAIDSGANINFDRLRHVAERAELGEQREAIIAVTVAERPGSFKAFCAAL
GRRQITEFNYRYHSDRQAHLFVGVQTHPLTDSRADLLAGLREQGFPVLDLTDNEMAKLHIRHMVGGHGTEVR
RERLFRFEFPERPGALLNFLDKLGSRWNISLFHYRNHGAADGRVLAGLQVPDEERGELEAALQAIGYPYWEE
THNPAYRLFAG*
AnnotationID:732GeneID:723
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 09:09:53
Gene Name:ilvA2
Confidence Code:2
GeneProduct:threonine dehydratase, biosynthetic
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:pyridoxal-phosphate dependent enzyme
Functional Category Confidence Code:5
COGs:COG1171,pfam00291
Secondary Functional Category(ies):
EC Number:4.2.1.16
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70714.1|AE016715_3 (AE016793) threonine dehydratase, biosynthetic [Pseudomonas putida
           KT2440]
          Length = 504

 Score =  775 bits (2000), Expect = 0.0
 Identities = 379/504 (75%), Positives = 438/504 (86%)

>gb|AAO58714.1| (AE016875) threonine dehydratase, biosynthetic [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 514

 Score =  773 bits (1996), Expect = 0.0
 Identities = 382/505 (75%), Positives = 438/505 (86%)

>dbj|BAB38129.1| (AP002566) threonine deaminase [Escherichia coli O157:H7]
          Length = 514

 Score =  551 bits (1420), Expect = e-156
 Identities = 280/513 (54%), Positives = 375/513 (73%), Gaps = 2/513 (0%)








 
Structural Features:
COG1171, IlvA, Threonine dehydratase 
[Amino acid transport and metabolism] 
CD-Length = 347 residues,  99.4% aligned
Score =  427 bits (1100), Expect = 1e-120
Identities = 192/346 (55%), Positives = 248/346 (71%), Gaps = 5/346 (1%)

pfam00291, PALP, Pyridoxal-phosphate dependent enzyme.
CD-Length = 298 residues, 100.0% aligned
Score =  231 bits (591), Expect = 1e-61
Identities = 114/303 (37%), Positives = 155/303 (51%), Gaps = 14/303 (4%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1449




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18591




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37454




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:723_cdd.html


    		




 
URL:

















    ReferenceID:38012




  
Author/Investigator(s):
        Cox JL, Cox BJ, Fidanza V, Calhoun DH.





  
Title:
        The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12.




  
PubMed:
        3315862


MedLine:
        




    
Source:
Gene. 1987;56(2-3):185-98.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3315862









           
Homologs By Global Alignment 

           

       



     
Gene ID:723
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1251
                           PA1326_tr
                           translation of PA1326
                           515
                           99.99
                           99.99
                           0
                           2622.0
     

     
     
47600
                           gb|AAG04715.1|AE004562_2
                           (AE004562) threonine dehydratase, biosynthetic [Pseudomonas aeruginosa PAO1]
                           515
                           99.99
                           99.99
                           0
                           2622.0
     

     
     
1252
                           PA0331_tr
                           translation of PA0331
                           515
                           77.66
                           87.57
                           11
                           2094.0
     

     
     
47601
                           gb|AAG03720.1|AE004471_3
                           (AE004471) threonine dehydratase, biosynthetic [Pseudomonas aeruginosa PAO1]
                           515
                           77.66
                           87.57
                           11
                           2094.0
     

     
     
47603
                           gb|AAO58714.1|
                           (AE016875) threonine dehydratase, biosynthetic [Pseudomonas syringae pv. tomato str. DC3000]
                           515
                           74.75
                           86.01
                           1
                           1997.0
     

     






      

          
GC ORFID: 43725How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1326Glimmer Score: 

          
Start: 756343Stop: 757890

          
Length: 1548


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1326 translation of PA1326Homolog Bit Score 1014.0

          
Other Homologs:  t_PA0331 (ORF: BLASTX 811.0), psyr_15may02_Scaffold6_revised_gene6529 (ORF: BLASTX 771.0), t_PA1326 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGCCTCGACCCTCAGCGCAAGCAAACCCCTGCTGGCCGGCTACGTGGAAAGCATCCTGGCCGCGCCG 
GTGTACGACGTCGCCGTGGAAACCCCCTTGCAGGTCGCGCCGCAGTTGTCCCAGCGCCTGGGCAACCGGGTG 
CTGCTCAAGCGCGAGGACCTGCAGCCGGTGTTCTCCTTCAAGATCCGCGGCGCCTATACCCGTGTCGCGCGC 
CTGAGCGACGAGCAGAAGGCCCGCGGGGTGATCACCGCCTCCGCCGGCAACCATGCCCAGGGCCTGGCCCTG 
GCGGCGCAGCGCCTGGGCGTGCGGGCGGTGATCGTGATGCCGCGCACCACTCCCGAGCTCAAGGTCAAGGGC 
GTGCTGGCGCGCGGCGGCGAGGCGTTGCTGCACGGCGACGCTTTCCCCGATGCGCTGGCCCATGCCCTGCAA 
CTGGCCGAGCGCGAAGGCATGACCTTCGTCCCGCCCTACGACGATCCGGACGTGATCGCCGGACAGGGCACG 
GTGGCGATGGAGATCCTCCGCCAGCACAGCGGCCGCCTCGACGCGATCTTCGTGCCGGTCGGCGGCGGCAGC 
CTGATTGCCGGGATCGCCGCCTACGTCAAGCACCTGCGTCCGGATATCCGCGTGATCGGCGTCGAGCCGGAG 
GATTCCAACTGCCTGCAGGCGGCGCTGGCCGCCGGCGAACGGGTGGTGCTGGGCCAGGTCGGGCTGTTCGCC 
GACGGGGTGGCGGTGGCGCAGATCGGCGCTTGCAACTTCGAGGTCTGCAAGGACCATGTCGACGAGGTGATC 
ACCGTCGGCAGCGACGAAATCTGCGCGGCGATCAAGGACATCTACGACGACACCCGTTCGATCACCGAGCCG 
GCCGGCGCCCTGGCCGTAGCGGGGATCAAGAAGTACGTGGCCCGCGAACGCACCGAGGGGCAGACCCTGGTG 
GCCATCGACTCCGGTGCCAACATCAACTTCGACCGCCTGCGCCACGTTGCCGAGCGCGCCGAACTGGGCGAA 
CAGCGCGAAGCGATCATCGCCGTCACCGTGGCCGAGCGGCCGGGCAGCTTCAAGGCCTTCTGCGCGGCGCTG 
GGCCGGCGCCAGATCACCGAGTTCAACTACCGCTACCACAGCGACCGCCAGGCCCACCTGTTCGTCGGCGTG 
CAGACCCATCCGCTCACCGACAGTCGCGCCGACCTGCTCGCCGGCCTGCGTGAGCAGGGCTTCCCGGTACTC 
GACCTGACCGACAACGAGATGGCCAAGCTGCACATCCGCCACATGGTCGGCGGCCACGGTACCGAGGTGCGC 
CGCGAGCGGCTGTTCCGCTTCGAGTTTCCCGAACGCCCCGGGGCCTTGCTGAACTTTCTCGACAAGCTCGGC 
TCGCGCTGGAACATCAGCCTGTTCCACTACCGCAACCACGGCGCCGCCGACGGTCGGGTGCTGGCCGGATTG 
CAGGTGCCGGACGAGGAGCGCGGCGAGCTGGAGGCGGCGTTGCAAGCCATCGGCTATCCGTACTGGGAGGAA 
ACCCACAACCCGGCCTACCGGCTGTTCGCCGGTTGA