ViewGene
Locus Name:
GeneID:
Location:

GeneID:721
TrivialName: PA14_00010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00010
PAO1 Ortholog Locus:PA0001
Sequence Length:1545
Protein Length:514
Start: 483
Stop: 2027
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03391.1|AE004440_1 (AE004440) chromosomal replication initiator protein DnaA [Pseudomonas aeruginosa PAO1]
Identities = 514/514 (100%), Positives = 514/514 (100%)
Sequence:
GTGTCCGTGGAACTTTGGCAGCAGTGCGTGGATCTTCTCCGCGATGAGCTGCCGTCCCAACAATTCAACACC
TGGATCCGTCCCTTGCAGGTCGAAGCCGAAGGCGACGAATTGCGTGTGTATGCACCCAACCGTTTCGTCCTC
GATTGGGTGAACGAGAAATACCTCGGTCGGCTTCTGGAACTGCTCGGTGAACGCGGCGAGGGTCAGTTGCCC
GCGCTTTCCTTATTAATAGGCAGCAAGCGTAGCCGTACGCCGCGCGCCGCCATCGTCCCATCGCAGACCCAC
GTGGCTCCCCCGCCTCCGGTTGCTCCGCCGCCGGCGCCAGTGCAGCCGGTATCGGCCGCGCCCGTGGTGGTG
CCACGTGAAGAGCTGCCGCCAGTGACGACGGCTCCCAGCGTGTCGAGCGACCCCTACGAGCCGGAAGAACCC
AGCATCGATCCGCTGGCCGCCGCCATGCCGGCCGGAGCCGCGCCTGCGGTACGCACCGAGCGCAACGTCCAG
GTCGAAGGCGCGCTGAAGCACACCAGCTATCTCAACCGTACCTTCACCTTCGAGAACTTCGTCGAGGGCAAG
TCCAACCAGTTGGCCCGTGCCGCCGCCTGGCAGGTGGCGGACAACCTCAAGCACGGCTACAACCCGCTGTTC
CTCTACGGTGGCGTCGGCCTGGGCAAGACCCACCTGATGCATGCGGTGGGCAACCACCTGCTGAAGAAGAAC
CCGAACGCCAAGGTGGTCTACCTGCATTCGGAACGTTTCGTCGCGGACATGGTGAAGGCCTTGCAGCTCAAC
GCCATCAACGAATTCAAGCGCTTCTACCGCTCGGTGGACGCACTGTTGATCGACGACATCCAGTTCTTCGCC
CGTAAGGAGCGCTCCCAGGAGGAGTTCTTCCACACCTTCAATGCCCTTCTCGAAGGCGGCCAGCAGGTGATC
CTCACCAGCGACCGCTATCCGAAGGAAATCGAAGGCCTGGAAGAGCGGCTGAAATCCCGCTTCGGCTGGGGC
CTGACGGTGGCCGTCGAGCCGCCGGAACTGGAAACCCGGGTGGCGATCCTGATGAAGAAGGCCGAGCAGGCG
AAGATCGAGCTGCCGCACGATGCGGCCTTCTTCATCGCCCAGCGCATCCGTTCCAACGTGCGTGAACTGGAA
GGTGCGCTGAAGCGGGTGATCGCCCATTCGCACTTCATGGGTCGGCCGATCACCATCGAGCTGATTCGCGAG
TCGCTGAAGGACCTGTTGGCCCTTCAGGACAAGCTGGTCAGCATCGACAACATCCAGCGCACCGTCGCCGAG
TACTACAAGATCAAGATATCCGATCTGTTGTCCAAGCGGCGTTCGCGTTCGGTGGCGCGCCCGCGCCAGGTG
GCCATGGCGCTCTCCAAGGAGCTGACCAACCACAGCCTGCCGGAGATCGGCGTGGCCTTCGGCGGTCGGGAT
CACACCACGGTGTTGCACGCCTGTCGTAAGATCGCTCAACTTAGGGAATCCGACGCGGATATCCGCGAGGAC
TACAAGAACCTGCTGCGTACCCTGACAACCTGA
Translation:
MSVELWQQCVDLLRDELPSQQFNTWIRPLQVEAEGDELRVYAPNRFVLDWVNEKYLGRLLELLGERGEGQLP
ALSLLIGSKRSRTPRAAIVPSQTHVAPPPPVAPPPAPVQPVSAAPVVVPREELPPVTTAPSVSSDPYEPEEP
SIDPLAAAMPAGAAPAVRTERNVQVEGALKHTSYLNRTFTFENFVEGKSNQLARAAAWQVADNLKHGYNPLF
LYGGVGLGKTHLMHAVGNHLLKKNPNAKVVYLHSERFVADMVKALQLNAINEFKRFYRSVDALLIDDIQFFA
RKERSQEEFFHTFNALLEGGQQVILTSDRYPKEIEGLEERLKSRFGWGLTVAVEPPELETRVAILMKKAEQA
KIELPHDAAFFIAQRIRSNVRELEGALKRVIAHSHFMGRPITIELIRESLKDLLALQDKLVSIDNIQRTVAE
YYKIKISDLLSKRRSRSVARPRQVAMALSKELTNHSLPEIGVAFGGRDHTTVLHACRKIAQLRESDADIRED
YKNLLRTLTT*
AnnotationID:730GeneID:721
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 09:09:07
Gene Name:dnaA
Confidence Code:2
GeneProduct:chromosomal replication initiator protein DnaA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00308,COG0593
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53558.1| (AE016856) chromosomal replication initiator protein DnaA
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 511

 Score =  840 bits (2169), Expect = 0.0
 Identities = 442/519 (85%), Positives = 461/519 (88%), Gaps = 13/519 (2%)

>gb|AAN65644.1|AE016191_1 (AE016774) chromosomal replication initiator protein DnaA
           [Pseudomonas putida KT2440]
          Length = 506

 Score =  827 bits (2136), Expect = 0.0
 Identities = 436/518 (84%), Positives = 453/518 (87%), Gaps = 16/518 (3%)

>gb|AAP18992.1| (AE016991) replication initiation protein DnaA [Shigella flexneri
           2a str. 2457T]
          Length = 467

 Score =  614 bits (1584), Expect = e-175
 Identities = 330/515 (64%), Positives = 386/515 (74%), Gaps = 49/515 (9%)






 
Structural Features:
pfam00308, Bac_DnaA, Bacterial dnaA protein.
CD-Length = 314 residues, 100.0% aligned
Score =  492 bits (1269), Expect = 4e-140
Identities = 195/314 (62%), Positives = 243/314 (77%), Gaps = 1/314 (0%)

COG0593, DnaA, ATPase involved in DNA replication initiation 
[DNA replication, recombination, and repair]
CD-Length = 408 residues,  82.1% aligned
Score =  459 bits (1183), Expect = 3e-130
Identities = 206/338 (60%), Positives = 256/338 (75%), Gaps = 3/338 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:14205




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18587




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33366




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:721_cdd.html


    		




 
URL:

















    ReferenceID:35539




  
Author/Investigator(s):
        Gasc AM, Giammarinaro P, Richter S, Sicard M.





  
Title:
        Organization around the dnaA gene of Streptococcus pneumoniae.




  
PubMed:
        9493380


MedLine:
        




    
Source:
Microbiology. 1998 Feb;144 ( Pt 2):433-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9493380

















    ReferenceID:35540




  
Author/Investigator(s):
        Old IG, Margarita D, Saint Girons I.





  
Title:
        Unique genetic arrangement in the dnaA region of the Borrelia burgdorferi linear chromosome: nucleotide sequence of the dnaA gene.




  
PubMed:
        8359672


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1993 Jul 15;111(1):109-14.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8359672

















    ReferenceID:35544




  
Author/Investigator(s):
        Clark MA, Baumann L, Baumann P.





  
Title:
        Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho.




  
PubMed:
        9516544


MedLine:
        




    
Source:
Curr Microbiol. 1998 Mar;36(3):158-63.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9516544

















    ReferenceID:35551




  
Author/Investigator(s):
        Fsihi H, De Rossi E, Salazar L, Cantoni R, Labo M, Riccardi G, Takiff HE, Eiglmeier K, Bergh S, Cole ST.





  
Title:
        Gene arrangement and organization in a approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae.




  
PubMed:
        8969512


MedLine:
        




    
Source:
Microbiology. 1996 Nov;142 ( Pt 11):3147-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8969512









           
Homologs By Global Alignment 

           

       



     
Gene ID:721
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1248
                           PA0001_tr
                           translation of PA0001
                           514
                           99.99
                           99.99
                           0
                           2624.0
     

     
     
47404
                           gb|AAG03391.1|AE004440_1
                           (AE004440) chromosomal replication initiator protein DnaA [Pseudomonas aeruginosa PAO1]
                           514
                           99.80
                           99.99
                           0
                           2621.0
     

     
     
47405
                           gb|AAG28797.1|AF229442_1
                           (AF229442) DnaA [Pseudomonas aeruginosa]
                           514
                           99.80
                           99.99
                           0
                           2621.0
     

     
     
47406
                           gb|AAO53558.1|
                           (AE016856) chromosomal replication initiator protein DnaA [Pseudomonas syringae pv. tomato str. DC3000]
                           519
                           84.97
                           88.82
                           13
                           2180.0
     

     
     
47407
                           gb|AAN65644.1|AE016191_1
                           (AE016774) chromosomal replication initiator protein DnaA [Pseudomonas putida KT2440]
                           527
                           82.73
                           85.95
                           34
                           2147.0
     

     






      

          
GC ORFID: 43723How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0001Glimmer Score: 

          
Start: 5801820Stop: 5803364

          
Length: 1545


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0001 translation of PA0001Homolog Bit Score 1015.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2966 (ORF: BLASTX 839.0), t_PA0001 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGTCCGTGGAACTTTGGCAGCAGTGCGTGGATCTTCTCCGCGATGAGCTGCCGTCCCAACAATTCAACACC 
TGGATCCGTCCCTTGCAGGTCGAAGCCGAAGGCGACGAATTGCGTGTGTATGCACCCAACCGTTTCGTCCTC 
GATTGGGTGAACGAGAAATACCTCGGTCGGCTTCTGGAACTGCTCGGTGAACGCGGCGAGGGTCAGTTGCCC 
GCGCTTTCCTTATTAATAGGCAGCAAGCGTAGCCGTACGCCGCGCGCCGCCATCGTCCCATCGCAGACCCAC 
GTGGCTCCCCCGCCTCCGGTTGCTCCGCCGCCGGCGCCAGTGCAGCCGGTATCGGCCGCGCCCGTGGTGGTG 
CCACGTGAAGAGCTGCCGCCAGTGACGACGGCTCCCAGCGTGTCGAGCGACCCCTACGAGCCGGAAGAACCC 
AGCATCGATCCGCTGGCCGCCGCCATGCCGGCCGGAGCCGCGCCTGCGGTACGCACCGAGCGCAACGTCCAG 
GTCGAAGGCGCGCTGAAGCACACCAGCTATCTCAACCGTACCTTCACCTTCGAGAACTTCGTCGAGGGCAAG 
TCCAACCAGTTGGCCCGTGCCGCCGCCTGGCAGGTGGCGGACAACCTCAAGCACGGCTACAACCCGCTGTTC 
CTCTACGGTGGCGTCGGCCTGGGCAAGACCCACCTGATGCATGCGGTGGGCAACCACCTGCTGAAGAAGAAC 
CCGAACGCCAAGGTGGTCTACCTGCATTCGGAACGTTTCGTCGCGGACATGGTGAAGGCCTTGCAGCTCAAC 
GCCATCAACGAATTCAAGCGCTTCTACCGCTCGGTGGACGCACTGTTGATCGACGACATCCAGTTCTTCGCC 
CGTAAGGAGCGCTCCCAGGAGGAGTTCTTCCACACCTTCAATGCCCTTCTCGAAGGCGGCCAGCAGGTGATC 
CTCACCAGCGACCGCTATCCGAAGGAAATCGAAGGCCTGGAAGAGCGGCTGAAATCCCGCTTCGGCTGGGGC 
CTGACGGTGGCCGTCGAGCCGCCGGAACTGGAAACCCGGGTGGCGATCCTGATGAAGAAGGCCGAGCAGGCG 
AAGATCGAGCTGCCGCACGATGCGGCCTTCTTCATCGCCCAGCGCATCCGTTCCAACGTGCGTGAACTGGAA 
GGTGCGCTGAAGCGGGTGATCGCCCATTCGCACTTCATGGGTCGGCCGATCACCATCGAGCTGATTCGCGAG 
TCGCTGAAGGACCTGTTGGCCCTTCAGGACAAGCTGGTCAGCATCGACAACATCCAGCGCACCGTCGCCGAG 
TACTACAAGATCAAGATATCCGATCTGTTGTCCAAGCGGCGTTCGCGTTCGGTGGCGCGCCCGCGCCAGGTG 
GCCATGGCGCTCTCCAAGGAGCTGACCAACCACAGCCTGCCGGAGATCGGCGTGGCCTTCGGCGGTCGGGAT 
CACACCACGGTGTTGCACGCCTGTCGTAAGATCGCTCAACTTAGGGAATCCGACGCGGATATCCGCGAGGAC 
TACAAGAACCTGCTGCGTACCCTGACAACCTGA