ViewGene
Locus Name:
GeneID:
Location:

GeneID:72
TrivialName: PA14_09520
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_09520
PAO1 Ortholog Locus:PA4207
Sequence Length:3090
Protein Length:1029
Start: 819395
Stop: 816306
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07594.1|AE004837_5 (AE004837) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
Identities = 1028/1029 (99%), Positives = 1028/1029 (99%)
Sequence:
ATGACCTTTACCGACCTGTTCGTCCGCCGGCCGGTGCTGGCGCTGGTGGTCAGCACGCTGATCCTGCTGCTC
GGCCTGTTCTCCCTGGGCAAGCTGCCGATCCGCCAGTACCCGCTGCTGGAAAGCTCGACCATCACCGTCACC
ACCGAGTACCCCGGCGCCTCCGCCGATCTCATGCAAGGCTTCGTCACCCAGCCGATCGCCCAGGCGGTGTCG
TCGGTGGAGGGCATCGACTACCTTTCCTCGACCTCGGTGCAGGGGCGTAGCGTGGTGACCATCCGCATGCTG
CTCAACCGCGATTCGACCCAGGCGATGACCGAGACCATGGCCAAGGTCAACTCGGTGCGCTACAAGCTGCCC
GAGCGTGCCTACGACTCGGTGATCGAACGCTCTTCCGGCGAGACCACCGCGGTGGCCTACGTCGGCTTTTCC
AGCAAGACCCTGCCGATCCCGGCGTTGACCGACTACCTGTCGCGGGTGGTCGAGCCGATGTTCTCTTCCATC
GACGGCGTGGCCAAGGTCCAGACCTTTGGCGGCCAGCGCCTGGCCATGCGCCTCTGGCTCGACGCCGACCGC
CTCGCCGGGCGCGGCCTGACCGCCTCCGACGTGGCCGAGGCGATCCGCCGCAACAACTACCAGGCGGCGCCG
GGGATGGTGAAGGGGCAGTACGTGCTGTCCAACGTGCGGGTCAACACCGACCTGACCAACGTCGACGACTTC
CGCGAGATGGTCATCCGCAACGATGGCAACGGCCTGGTGCGCCTGCGCGACGTCGGTACCGTCGAACTGGGC
GCCGCGGCCACCGAGACCAGCGCACTGATGGACGGCGACCCGGCGGTGCACCTGGGGTTGTTCCCGACGCCC
ACCGGCAACCCGCTGGTGATCGTCGACGGCATCCGCAAGCTGCTGCCGGAGATCCAGAAGACCCTGCCGCCG
GATGTCCGCGTCGACCTCGCCTACGAGACTTCGCGCTTCATCCAGGCCTCCATCGACGAGGTGGTGCGGACC
CTGGTGGAAGCGCTGCTGATCGTGGTGCTGGTGATCTACCTCTGCCTCGGCTCGCTGCGCAGCGTGCTGATC
CCGGTGGCGACCATTCCCCTGTCGATGCTCGGCGCCGCCGCGCTGATGCTGGCCTTCGGCTTCAGCGTCAAC
CTGCTGACCCTGCTGGCGATGGTGCTGGCCATCGGGCTGGTGGTGGACGACGCCATCGTGGTGGTGGAGAAC
GTCCACCGCCACATCGAGGAAGGCAAGTCGCCGGTGGCGGCGGCGCTGATCGGCGCCCGCGAAGTGGCCGGC
CCGGTGATCGCCATGACCATCACCCTGGCCGCCGTGTACACCCCCATCGGCCTGATGGGCGGCCTCACCGGC
GCACTGTTCCGCGAGTTCGCCCTGACCCTGGCGGGCGCGGTGATCGTGTCCGGGGTGGTGGCGCTGACCCTG
TCGCCGGTGATGAGTTCGCTGCTGCTCCAGGCGCACCAGAACGAGGGGCGCATGGGCCGCGCCGCCGAGTGG
TTCTTCGGCGGCCTGACGCGGCGCTACGGGCAGGTCCTGGAGTTCTCCCTGGGCCACCGCTGGCTGACCGGC
GGCCTGGCCTTGCTGGTGTGCATCAGCCTGCCGCTGCTGTATTCGATGCCCAAGCGCGAACTGGCGCCGACC
GAGGACCAAGCCGCGGTGCTCACCGCGATCAAGGCGCCGCAGCACGCCAACCTCGACTACGTCGAACTGTTC
GCGCGCAAGCTCGACCAGGTCTACACCAGCATCCCGGAAACCGTGAGCACCTGGATCATCAACGGCACCGAC
GGCCCGGCGGCGAGCTTCGGCGGGATCAACCTGGCGGCCTGGGAAAAACGCGAGCGCGACGCCTCGGCGATC
CAGTCCGAGCTGCAAGGCAAGGTCGGCGACGTCGAGGGCAGCAGCATCTTCGCCTTCCAGTTGGCCGCCCTG
CCCGGCTCCACCGGCGGCCTGCCGGTGCAGATGGTGCTGCGCAGCCCGCAGGACTATCCGGTGCTCTACCGG
ACCATGGAAGAGATCAAGCAGAAGGCCCGGCAGAGCGGGCTGTTCGTGGTGGTCGACAGCGACCTCGACTAC
AACAACCCGGTGGTCCAGGTCCGCATCGACCGCGCCAAGGCCAACAGCCTGGGCATCCGCATGCAGGACATC
GGCGAGTCGCTGGCGGTGCTGGTGGGCGAGAACTACGTCAACCGCTTCGGCATGGAAGGCCGCTCCTACGAC
GTGATCCCGCAGAGCCTGCGCGACCAGCGTTTCACTCCGCAAGCGCTGGCACGACAGTTCGTGCGCACCCAG
GACGGCAACCTGGTGCCGCTGTCGACGGTGGTCCGGGTGGAGCTTCAGGTCGAACCGAACAAGCTGATCCAG
TTCGACCAGCAGAACGCCGCGACCCTCCAGGCGATCCCCGCGCCCGGCGTCTCCATGGGCCAGGCGGTGGCC
TTCCTCGACGACGTGGCGCGCGGCCTGCCGGCCGGCTTCAGCCACGACTGGCAATCCGACTCGCGGCAATAC
ACCCAGGAAGGCAACACCCTGGTGTTCGCCTTCCTCGCCGCCCTGGTGGTGATCTACCTGGTGCTCGCCGCG
CAGTACGAGAGCCTGGCCGACCCGCTGATCATCCTGATCACCGTGCCGCTGTCGATCTGCGGCGCGCTGCTG
CCGCTGGCGCTGGGCTACGCGACGATGAACATCTATACGCAGATCGGCCTGGTCACCCTGATCGGCCTGATC
AGCAAGCACGGCATCCTCATGGTCGAGTTCGCCAACGAACTGCAACTCCACGAGCGCCTCGACCGCCGCGCG
GCGATCCTGCGCGCCGCACAGATCCGCCTGCGGCCGGTGCTGATGACCACCGCGGCAATGGTCTTCGGCCTG
GTGCCGCTGCTCTTCGCCAGCGGCGCCGGCGCCGCCAGCCGCTTCGGCCTGGGCGTGGTGATCGTCTCCGGG
ATGCTGGTCGGCACCCTCTTCACCCTGTTCGTGCTGCCCACCGTCTATACCCTGCTGGCGCGCAACCACGCG
GAAGTCGACAAGAGCCCGCGCAGCCGGCAACTGGCCGAGGCCGATCTGCTGGTGAACAAGGCATGA
Translation:
MTFTDLFVRRPVLALVVSTLILLLGLFSLGKLPIRQYPLLESSTITVTTEYPGASADLMQGFVTQPIAQAVS
SVEGIDYLSSTSVQGRSVVTIRMLLNRDSTQAMTETMAKVNSVRYKLPERAYDSVIERSSGETTAVAYVGFS
SKTLPIPALTDYLSRVVEPMFSSIDGVAKVQTFGGQRLAMRLWLDADRLAGRGLTASDVAEAIRRNNYQAAP
GMVKGQYVLSNVRVNTDLTNVDDFREMVIRNDGNGLVRLRDVGTVELGAAATETSALMDGDPAVHLGLFPTP
TGNPLVIVDGIRKLLPEIQKTLPPDVRVDLAYETSRFIQASIDEVVRTLVEALLIVVLVIYLCLGSLRSVLI
PVATIPLSMLGAAALMLAFGFSVNLLTLLAMVLAIGLVVDDAIVVVENVHRHIEEGKSPVAAALIGAREVAG
PVIAMTITLAAVYTPIGLMGGLTGALFREFALTLAGAVIVSGVVALTLSPVMSSLLLQAHQNEGRMGRAAEW
FFGGLTRRYGQVLEFSLGHRWLTGGLALLVCISLPLLYSMPKRELAPTEDQAAVLTAIKAPQHANLDYVELF
ARKLDQVYTSIPETVSTWIINGTDGPAASFGGINLAAWEKRERDASAIQSELQGKVGDVEGSSIFAFQLAAL
PGSTGGLPVQMVLRSPQDYPVLYRTMEEIKQKARQSGLFVVVDSDLDYNNPVVQVRIDRAKANSLGIRMQDI
GESLAVLVGENYVNRFGMEGRSYDVIPQSLRDQRFTPQALARQFVRTQDGNLVPLSTVVRVELQVEPNKLIQ
FDQQNAATLQAIPAPGVSMGQAVAFLDDVARGLPAGFSHDWQSDSRQYTQEGNTLVFAFLAALVVIYLVLAA
QYESLADPLIILITVPLSICGALLPLALGYATMNIYTQIGLVTLIGLISKHGILMVEFANELQLHERLDRRA
AILRAAQIRLRPVLMTTAAMVFGLVPLLFASGAGAASRFGLGVVIVSGMLVGTLFTLFVLPTVYTLLARNHA
EVDKSPRSRQLAEADLLVNKA*
AnnotationID:82GeneID:72
AnnotatorUID: diggins
Modification Date/Time: 2005-06-06 14:02:50
Gene Name:mexI
Confidence Code:1
GeneProduct:probable RND efflux transporter
Cell Localization:(4) Inner membrane protein
Synonyms:
Cell Localization Confidence Code:2
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00873,COG0841
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67679.1|AE016398_3 (AE016781) multidrug efflux RND transporter [Pseudomonas putida
            KT2440]
          Length = 1027

 Score = 1556 bits (4029), Expect = 0.0
 Identities = 791/1023 (77%), Positives = 915/1023 (89%), Gaps = 2/1023 (0%)

>emb|CAE35827.1| (BX640448) integral membrane component of multidrug efflux system
            [Bordetella bronchiseptica]
          Length = 1037

 Score = 1399 bits (3622), Expect = 0.0
 Identities = 717/1023 (70%), Positives = 848/1023 (82%)

>dbj|BAC58301.1| (AP005073) putative multidrug resistance protein [Vibrio
            parahaemolyticus]
          Length = 1040

 Score =  948 bits (2451), Expect = 0.0
 Identities = 469/1008 (46%), Positives = 697/1008 (69%), Gaps = 1/1008 (0%)






 
Structural Features:
pfam00873, ACR_tran, AcrB/AcrD/AcrF family.
CD-Length = 1017 residues, 100.0% aligned
Score =  820 bits (2119), Expect = 0.0
Identities = 385/1018 (37%), Positives = 582/1018 (57%), Gaps = 17/1018 (1%)

COG0841, AcrB, Cation/multidrug efflux pump [Defense mechanisms]
CD-Length = 1009 residues,  99.8% aligned
Score =  893 bits (2309), Expect = 0.0
Identities = 403/1012 (39%), Positives = 598/1012 (59%), Gaps = 14/1012 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:147




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17289




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36917




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:72_cdd.html


    		




 
URL:

















    ReferenceID:37007




  
Author/Investigator(s):
        Aendekerk S, Ghysels B, Cornelis P, Baysse C.





  
Title:
        Characterization of a new efflux pump, MexGHI-OpmD, from Pseudomonas aeruginosa that confers resistance to vanadium.




  
PubMed:
        12177331


MedLine:
        




    
Source:
Microbiology. 2002 Aug;148(Pt 8):2371-81.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12177331









           
Homologs By Global Alignment 

           

       



     
Gene ID:72
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
146
                           PA4207_tr
                           translation of PA4207
                           1029
                           99.90
                           99.90
                           0
                           5079.0
     

     
     
13474
                           gb|AAG07594.1|AE004837_5
                           (AE004837) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
                           1029
                           99.90
                           99.90
                           0
                           5079.0
     

     
     
13475
                           gb|AAN67679.1|AE016398_3
                           (AE016781) multidrug efflux RND transporter [Pseudomonas putida KT2440]
                           1029
                           76.87
                           89.01
                           2
                           4026.0
     

     
     
13476
                           emb|CAE35827.1|
                           (BX640448) integral membrane component of multidrug efflux system [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           1037
                           69.23
                           81.87
                           8
                           3622.0
     

     
     
13477
                           emb|CAE41130.1|
                           (BX640413) integral membrane component of multidrug efflux system [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           1037
                           68.85
                           81.77
                           8
                           3605.0
     

     






      

          
GC ORFID: 43074How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4207Glimmer Score: 

          
Start: 4134542Stop: 4137631

          
Length: 3090


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4207 translation of PA4207Homolog Bit Score 1961.0

          
Other Homologs:  t_PA4375 (ORF: BLASTX 983.0), t_PA4207 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCTTTACCGACCTGTTCGTCCGCCGGCCGGTGCTGGCGCTGGTGGTCAGCACGCTGATCCTGCTGCTC 
GGCCTGTTCTCCCTGGGCAAGCTGCCGATCCGCCAGTACCCGCTGCTGGAAAGCTCGACCATCACCGTCACC 
ACCGAGTACCCCGGCGCCTCCGCCGATCTCATGCAAGGCTTCGTCACCCAGCCGATCGCCCAGGCGGTGTCG 
TCGGTGGAGGGCATCGACTACCTTTCCTCGACCTCGGTGCAGGGGCGTAGCGTGGTGACCATCCGCATGCTG 
CTCAACCGCGATTCGACCCAGGCGATGACCGAGACCATGGCCAAGGTCAACTCGGTGCGCTACAAGCTGCCC 
GAGCGTGCCTACGACTCGGTGATCGAACGCTCTTCCGGCGAGACCACCGCGGTGGCCTACGTCGGCTTTTCC 
AGCAAGACCCTGCCGATCCCGGCGTTGACCGACTACCTGTCGCGGGTGGTCGAGCCGATGTTCTCTTCCATC 
GACGGCGTGGCCAAGGTCCAGACCTTTGGCGGCCAGCGCCTGGCCATGCGCCTCTGGCTCGACGCCGACCGC 
CTCGCCGGGCGCGGCCTGACCGCCTCCGACGTGGCCGAGGCGATCCGCCGCAACAACTACCAGGCGGCGCCG 
GGGATGGTGAAGGGGCAGTACGTGCTGTCCAACGTGCGGGTCAACACCGACCTGACCAACGTCGACGACTTC 
CGCGAGATGGTCATCCGCAACGATGGCAACGGCCTGGTGCGCCTGCGCGACGTCGGTACCGTCGAACTGGGC 
GCCGCGGCCACCGAGACCAGCGCACTGATGGACGGCGACCCGGCGGTGCACCTGGGGTTGTTCCCGACGCCC 
ACCGGCAACCCGCTGGTGATCGTCGACGGCATCCGCAAGCTGCTGCCGGAGATCCAGAAGACCCTGCCGCCG 
GATGTCCGCGTCGACCTCGCCTACGAGACTTCGCGCTTCATCCAGGCCTCCATCGACGAGGTGGTGCGGACC 
CTGGTGGAAGCGCTGCTGATCGTGGTGCTGGTGATCTACCTCTGCCTCGGCTCGCTGCGCAGCGTGCTGATC 
CCGGTGGCGACCATTCCCCTGTCGATGCTCGGCGCCGCCGCGCTGATGCTGGCCTTCGGCTTCAGCGTCAAC 
CTGCTGACCCTGCTGGCGATGGTGCTGGCCATCGGGCTGGTGGTGGACGACGCCATCGTGGTGGTGGAGAAC 
GTCCACCGCCACATCGAGGAAGGCAAGTCGCCGGTGGCGGCGGCGCTGATCGGCGCCCGCGAAGTGGCCGGC 
CCGGTGATCGCCATGACCATCACCCTGGCCGCCGTGTACACCCCCATCGGCCTGATGGGCGGCCTCACCGGC 
GCACTGTTCCGCGAGTTCGCCCTGACCCTGGCGGGCGCGGTGATCGTGTCCGGGGTGGTGGCGCTGACCCTG 
TCGCCGGTGATGAGTTCGCTGCTGCTCCAGGCGCACCAGAACGAGGGGCGCATGGGCCGCGCCGCCGAGTGG 
TTCTTCGGCGGCCTGACGCGGCGCTACGGGCAGGTCCTGGAGTTCTCCCTGGGCCACCGCTGGCTGACCGGC 
GGCCTGGCCTTGCTGGTGTGCATCAGCCTGCCGCTGCTGTATTCGATGCCCAAGCGCGAACTGGCGCCGACC 
GAGGACCAAGCCGCGGTGCTCACCGCGATCAAGGCGCCGCAGCACGCCAACCTCGACTACGTCGAACTGTTC 
GCGCGCAAGCTCGACCAGGTCTACACCAGCATCCCGGAAACCGTGAGCACCTGGATCATCAACGGCACCGAC 
GGCCCGGCGGCGAGCTTCGGCGGGATCAACCTGGCGGCCTGGGAAAAACGCGAGCGCGACGCCTCGGCGATC 
CAGTCCGAGCTGCAAGGCAAGGTCGGCGACGTCGAGGGCAGCAGCATCTTCGCCTTCCAGTTGGCCGCCCTG 
CCCGGCTCCACCGGCGGCCTGCCGGTGCAGATGGTGCTGCGCAGCCCGCAGGACTATCCGGTGCTCTACCGG 
ACCATGGAAGAGATCAAGCAGAAGGCCCGGCAGAGCGGGCTGTTCGTGGTGGTCGACAGCGACCTCGACTAC 
AACAACCCGGTGGTCCAGGTCCGCATCGACCGCGCCAAGGCCAACAGCCTGGGCATCCGCATGCAGGACATC 
GGCGAGTCGCTGGCGGTGCTGGTGGGCGAGAACTACGTCAACCGCTTCGGCATGGAAGGCCGCTCCTACGAC 
GTGATCCCGCAGAGCCTGCGCGACCAGCGTTTCACTCCGCAAGCGCTGGCACGACAGTTCGTGCGCACCCAG 
GACGGCAACCTGGTGCCGCTGTCGACGGTGGTCCGGGTGGAGCTTCAGGTCGAACCGAACAAGCTGATCCAG 
TTCGACCAGCAGAACGCCGCGACCCTCCAGGCGATCCCCGCGCCCGGCGTCTCCATGGGCCAGGCGGTGGCC 
TTCCTCGACGACGTGGCGCGCGGCCTGCCGGCCGGCTTCAGCCACGACTGGCAATCCGACTCGCGGCAATAC 
ACCCAGGAAGGCAACACCCTGGTGTTCGCCTTCCTCGCCGCCCTGGTGGTGATCTACCTGGTGCTCGCCGCG 
CAGTACGAGAGCCTGGCCGACCCGCTGATCATCCTGATCACCGTGCCGCTGTCGATCTGCGGCGCGCTGCTG 
CCGCTGGCGCTGGGCTACGCGACGATGAACATCTATACGCAGATCGGCCTGGTCACCCTGATCGGCCTGATC 
AGCAAGCACGGCATCCTCATGGTCGAGTTCGCCAACGAACTGCAACTCCACGAGCGCCTCGACCGCCGCGCG 
GCGATCCTGCGCGCCGCACAGATCCGCCTGCGGCCGGTGCTGATGACCACCGCGGCAATGGTCTTCGGCCTG 
GTGCCGCTGCTCTTCGCCAGCGGCGCCGGCGCCGCCAGCCGCTTCGGCCTGGGCGTGGTGATCGTCTCCGGG 
ATGCTGGTCGGCACCCTCTTCACCCTGTTCGTGCTGCCCACCGTCTATACCCTGCTGGCGCGCAACCACGCG 
GAAGTCGACAAGAGCCCGCGCAGCCGGCAACTGGCCGAGGCCGATCTGCTGGTGAACAAGGCATGA