ViewGene
Locus Name:
GeneID:
Location:

GeneID:718
TrivialName: PA14_23560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23560
PAO1 Ortholog Locus:PA3134
Sequence Length:1485
Protein Length:494
Start: 2051388
Stop: 2052872
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAD33773.1|AF139107_3 (AF139107) glutamyl-tRNA synthetase [Pseudomonas aeruginosa]
Identities = 494/494 (100%), Positives = 494/494 (100%)
Sequence:
ATGACCACTGTTCGTACTCGCATCGCCCCGTCGCCGACGGGCGACCCGCACGTCGGTACCGCATACATCGCG
CTGTTCAACCTGTGCTTCGCGCGTCAGCACGGCGGTCAGTTCATCCTGCGCATCGAGGACACCGACCAGCTG
CGTTCGACGCGGGAGTCCGAGCAGCAGATCTACGACGCCCTGCGCTGGCTCGGCATCGAGTGGGACGAGGGC
CCGGACGTCGGCGGCCCGCATGGCCCGTACCGGCAGAGCGAGCGCGGGCACATCTACAAGAGGTATTCGGAC
GAACTGGTCGAGAAGGGCCATGCCTTCACCTGCTTCTGCACGCCCGAGCGCCTCGATGCGGTTCGCGCCGAG
CAGATGGCGCGCAAGGAAACCCCGCGCTACGACGGCCACTGCATGCACCTGCCGAAGGATGAGGTGCAGCGC
CGCCTCGCCGCCGGCGAGTCCCATGTCACCCGCATGAAGGTGCCGACCGAGGGTGTCTGCGTGGTGCCGGAC
ATGCTCCGCGGCGATGTCGAGATTCCGTGGGATCGCATGGACATGCAGGTGCTGATGAAGGCCGACGGCCTG
CCCACCTACTTCCTCGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTCCTGCGCGGCGAAGAG
TGGCTGCCGTCGGCGCCCAAGCTGATCAAGCTCTACGAGTATTTCGGCTGGGAGCAGCCGCAGCTGTGCTAC
ATGCCGCTGCTGCGCAACCCGGACAAGAGCAAGCTGTCCAAGCGCAAGAACCCGACCTCTATCACCTTCTAC
GAACGCATGGGCTACCTGCCGCAGGCGCTGCTCAACTACCTTGGGCGGATGGGCTGGTCGATGCCGGACGAG
CGCGAGAAATTCACCCTCGCCGAGATGATCGAGCATTTCGACCTGTCGCGCGTCTCCCTGGGCGGACCGATC
TTCGACCTGGAGAAGCTCTCCTGGCTGAACGGGCAGTGGATCCGCGAGCAGTCCGTGGAGGAGTTCGCCCGT
GAGGTGCAGAAGTGGGCGTTGAATCCCGAATACCTGATGAAGATCGCTCCGCACGTGCAGGGACGGGTGGAG
AACTTCAGCCAGATCGCACCGCTGGCCGGATTCTTCTTCAGCGGCGGGGTGCCGCTGGACGCTTCGCTGTTC
GAACACAAGAAGCTCGATCCGACCCAGGTCCGCCAGGTTCTGCAACTGGTGCTGTGGAAACTCGAATCCCTG
CGCCAGTGGGAGAAGGAACGCATCACCGGCTGCATCCAGGCTGTCGCCGAACACCTCCAGCTCAAGCTGCGC
GACGTGATGCCGCTGATGTTCCCGGCCATCACCGGGCATGCCAGCTCGGTTTCGGTGCTGGACGCCATGGAG
ATCCTTGGTGCCGACCTGTCGCGCTATCGCTTGCGCCAGGCCCTGGAGCTACTGGGCGGGGCGTCGAAAAAG
GAAACCAAGGAGTGGGAGAAGATCCGCGACGCCATTCCCGGCTGA
Translation:
MTTVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIEDTDQLRSTRESEQQIYDALRWLGIEWDEG
PDVGGPHGPYRQSERGHIYKRYSDELVEKGHAFTCFCTPERLDAVRAEQMARKETPRYDGHCMHLPKDEVQR
RLAAGESHVTRMKVPTEGVCVVPDMLRGDVEIPWDRMDMQVLMKADGLPTYFLANVVDDHLMGITHVLRGEE
WLPSAPKLIKLYEYFGWEQPQLCYMPLLRNPDKSKLSKRKNPTSITFYERMGYLPQALLNYLGRMGWSMPDE
REKFTLAEMIEHFDLSRVSLGGPIFDLEKLSWLNGQWIREQSVEEFAREVQKWALNPEYLMKIAPHVQGRVE
NFSQIAPLAGFFFSGGVPLDASLFEHKKLDPTQVRQVLQLVLWKLESLRQWEKERITGCIQAVAEHLQLKLR
DVMPLMFPAITGHASSVSVLDAMEILGADLSRYRLRQALELLGGASKKETKEWEKIRDAIPG*
AnnotationID:727GeneID:718
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 16:04:00
Gene Name:gltx
Confidence Code:1
GeneProduct: glutamyl-tRNA synthetase
Cell Localization:()
Synonyms:PA3134
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00808,pfam00749,COG0008
Secondary Functional Category(ies):
EC Number:6.1.1.17
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO55683.1| (AE016863) glutamyl-tRNA synthetase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 493

 Score =  866 bits (2237), Expect = 0.0
 Identities = 411/492 (83%), Positives = 447/492 (90%)

>gb|AAC35209.1| (M27221) glutamyl-tRNA synthetase [Sinorhizobium meliloti]
          Length = 484

 Score =  493 bits (1270), Expect = e-139
 Identities = 243/474 (51%), Positives = 325/474 (68%), Gaps = 2/474 (0%)

>gb|AAF38063.1| (AE002180) glutamyl-tRNA synthetase [Chlamydophila pneumoniae AR39]
          Length = 505

 Score =  436 bits (1120), Expect = e-121
 Identities = 226/479 (47%), Positives = 303/479 (63%), Gaps = 2/479 (0%)








 
Structural Features:
cd00808, GluRS_core, Descriminating Glutamyl-tRNA synthetase (GluRS) catalytic core domain.
CD-Length = 326 residues, 100.0% aligned
Score =  516 bits (1331), Expect = 2e-147

pfam00749, tRNA-synt_1c, tRNA synthetases class I (E and Q), catalytic domain.
CD-Length = 314 residues, 100.0% aligned
Score =  372 bits (956), Expect = 6e-104
Identities = 151/322 (46%), Positives = 197/322 (61%), Gaps = 12/322 (3%)
 
COG0008, GlnS, Glutamyl- and glutaminyl-tRNA synthetases 
[Translation, ribosomal structure and biogenesis]
CD-Length = 472 residues,  98.5% aligned
Score =  461 bits (1187), Expect = 9e-131
Identities = 193/483 (39%), Positives = 255/483 (52%), Gaps = 29/483 (6%)








   
Genomic Context:






   
Comment:


















    ReferenceID:1439




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18581




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32388




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:718_cdd.html


    		




 
URL:

















    ReferenceID:33602




  
Author/Investigator(s):
        Franklund CV, Goldberg JB.





  
Title:
        Cloning of the glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa.




  
PubMed:
        10348873


MedLine:
        




    
Source:
J Bacteriol. 1999 Jun;181(11):3582-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10348873









           
Homologs By Global Alignment 

           

       



     
Gene ID:718
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
47349
                           gb|AAD33773.1|AF139107_3
                           (AF139107) glutamyl-tRNA synthetase [Pseudomonas aeruginosa]
                           494
                           99.99
                           99.99
                           0
                           2632.0
     

     
     
1244
                           PA3134_tr
                           translation of PA3134
                           494
                           99.79
                           99.99
                           0
                           2629.0
     

     
     
47350
                           gb|AAG06522.1|AE004737_8
                           (AE004737) glutamyl-tRNA synthetase [Pseudomonas aeruginosa PAO1]
                           494
                           99.79
                           99.99
                           0
                           2629.0
     

     
     
47351
                           gb|AAO55683.1|
                           (AE016863) glutamyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           494
                           83.19
                           90.48
                           1
                           2236.0
     

     
     
47352
                           gb|AAN67592.1|AE016388_12
                           (AE016781) glutamyl-tRNA synthetase [Pseudomonas putida KT2440]
                           494
                           82.79
                           90.28
                           1
                           2225.0
     

     






      

          
GC ORFID: 43720How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3134Glimmer Score: 

          
Start: 2902543Stop: 2901059

          
Length: 1485


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3134 translation of PA3134Homolog Bit Score 1017.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene963 (ORF: BLASTX 860.0), t_PA3134 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCACTGTTCGTACTCGCATCGCCCCGTCGCCGACGGGCGACCCGCACGTCGGTACCGCATACATCGCG 
CTGTTCAACCTGTGCTTCGCGCGTCAGCACGGCGGTCAGTTCATCCTGCGCATCGAGGACACCGACCAGCTG 
CGTTCGACGCGGGAGTCCGAGCAGCAGATCTACGACGCCCTGCGCTGGCTCGGCATCGAGTGGGACGAGGGC 
CCGGACGTCGGCGGCCCGCATGGCCCGTACCGGCAGAGCGAGCGCGGGCACATCTACAAGAGGTATTCGGAC 
GAACTGGTCGAGAAGGGCCATGCCTTCACCTGCTTCTGCACGCCCGAGCGCCTCGATGCGGTTCGCGCCGAG 
CAGATGGCGCGCAAGGAAACCCCGCGCTACGACGGCCACTGCATGCACCTGCCGAAGGATGAGGTGCAGCGC 
CGCCTCGCCGCCGGCGAGTCCCATGTCACCCGCATGAAGGTGCCGACCGAGGGTGTCTGCGTGGTGCCGGAC 
ATGCTCCGCGGCGATGTCGAGATTCCGTGGGATCGCATGGACATGCAGGTGCTGATGAAGGCCGACGGCCTG 
CCCACCTACTTCCTCGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTCCTGCGCGGCGAAGAG 
TGGCTGCCGTCGGCGCCCAAGCTGATCAAGCTCTACGAGTATTTCGGCTGGGAGCAGCCGCAGCTGTGCTAC 
ATGCCGCTGCTGCGCAACCCGGACAAGAGCAAGCTGTCCAAGCGCAAGAACCCGACCTCTATCACCTTCTAC 
GAACGCATGGGCTACCTGCCGCAGGCGCTGCTCAACTACCTTGGGCGGATGGGCTGGTCGATGCCGGACGAG 
CGCGAGAAATTCACCCTCGCCGAGATGATCGAGCATTTCGACCTGTCGCGCGTCTCCCTGGGCGGACCGATC 
TTCGACCTGGAGAAGCTCTCCTGGCTGAACGGGCAGTGGATCCGCGAGCAGTCCGTGGAGGAGTTCGCCCGT 
GAGGTGCAGAAGTGGGCGTTGAATCCCGAATACCTGATGAAGATCGCTCCGCACGTGCAGGGACGGGTGGAG 
AACTTCAGCCAGATCGCACCGCTGGCCGGATTCTTCTTCAGCGGCGGGGTGCCGCTGGACGCTTCGCTGTTC 
GAACACAAGAAGCTCGATCCGACCCAGGTCCGCCAGGTTCTGCAACTGGTGCTGTGGAAACTCGAATCCCTG 
CGCCAGTGGGAGAAGGAACGCATCACCGGCTGCATCCAGGCTGTCGCCGAACACCTCCAGCTCAAGCTGCGC 
GACGTGATGCCGCTGATGTTCCCGGCCATCACCGGGCATGCCAGCTCGGTTTCGGTGCTGGACGCCATGGAG 
ATCCTTGGTGCCGACCTGTCGCGCTATCGCTTGCGCCAGGCCCTGGAGCTACTGGGCGGGGCGTCGAAAAAG 
GAAACCAAGGAGTGGGAGAAGATCCGCGACGCCATTCCCGGCTGA