ViewGene
Locus Name:
GeneID:
Location:

GeneID:71
TrivialName: PA14_01970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_01970
PAO1 Ortholog Locus:PA0158
Sequence Length:3048
Protein Length:1015
Start: 178382
Stop: 181429
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03548.1|AE004453_11 (AE004453) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
Identities = 1013/1015 (99%), Positives = 1015/1015 (100%)
Sequence:
ATGAAGGGCGGTTTCAACCTGTCGGACTGGGCCCTGCGCCACCAGTCCCTGGTCTGGTACCTGATGGCGGTA
TCGCTGGTGATGGGCGTGTTCTCCTACCTCAACCTGGGGCGCGAGGAGGATCCCTCGTTCGCCATCAAGACC
ATGGTCATCCAGACCCGCTGGCCGGGCGCCACGGTGGACGACACCCTGGAGCAGGTCACCGACCGCATCGAG
AAGAAGCTCGAGGAGCTGGACTCGCTGGATTACGTGAAGAGCTACACGCGACCCGGCGAATCGACGGTCTTC
GTCTACCTCAAGGACACCACCAAGGCCGGCGACATCCCGGATATCTGGTACCAGGTGCGCAAGAAGATCTCC
GACATCCAGGGCGAATTCCCCCAAGGCATCCAGGGACCGGGTTTCAACGACGAGTTCGGCGACGTGTTCGGC
AGCGTCTACGCCTTCACCGCCGACGGCCTGGACTTCCGCCAGCTGCGCGACTACGTGGAGAAGGTGCGCCTG
GACATCCGCTCGGTGAAAGACCTGGGCAAGGTGCAGATGATCGGTGCGCAGAACGAGGTCATCTACCTCAAC
TTCTCCACCCGCAAGCTGGCCGCCCTCGGTCTCGACCAGCGCCAGGTCGTGCAGAGCCTGCAAGCGCAGAAC
GCGGTGACCCCGTCCGGCGTGGTCGAGGCCGGCCCCGAGCGCATCTCGGTGCGCACCTCCGGCAACTTCCGT
TCGGAAAAGGACCTGCAGGCGGTCAACCTGCGGGTCAACGATCGTTTCTACCGGCTGTCCGACCTAGCCAGC
ATCAGCCGCGATTTCGTCGACCCGCCGACCTCGCTGTTCCGCTACAAGGGCGAGCCGGCCATCGGCCTGGCG
GTGGCGATGAAGGAGGGCGGCAATATCCTCGAGTTCGGCGAGGCGCTCAATGCGCGCATGCAGGAGATCACC
GGCGAACTGCCGGTCGGGGTCGGCGTGCACCAGGTGTCGAACCAGGCCCAGGTGGTGAAGAAGGCGGTCGGC
GGTTTCACCCAGGCGCTGTTCGAGGCGGTGGTGATCGTCCTCATCGTCAGCTTCGTCAGCCTCGGCCTGCGC
GCCGGGCTGGTGGTGGCCTGCTCGATCCCGCTGGTGCTGGCGATGGTCTTCGTGTTCATGGAATACACCGAC
ATCACCATGCAGCGGGTTTCCCTCGGCGCGCTGATCATCGCCCTCGGCCTGCTGGTGGACGATGCCATGATC
ACCGTGGAGATGATGATCACGCGCCTCGAACTGGGCGACTCGCTGCACGACTCGGCGACCTACGCCTACACC
TCGACGGCCTTCCCGATGCTCACCGGGACCCTGGTGACGGTGGCCGGCTTCGTGCCCATCGGGCTCAACGCC
AGCTCCGCCGGCGAGTACACCTTCACCCTGTTCGCGGTGATCGCCGTGGCCCTGCTGCTGTCGTGGATCGTC
GCGGTGCTGTTCGCCCCGGTGATCGCCGTGCACATCCTGCCGAAGACTCTCAAGCACAAGTCGGAGCAGAAG
AAGGGGCGCATCGCCGAGCGTTTCGACAGCCTGCTGCACCTGGCGATGCGCCGGCGCTGGACGACCATCTTC
CTCACCGCGCTGCTGTTCGGCGTGTCGCTGTTCCTGATGAAGTTCGTCCAGCACCAGTTCTTCCCGTCTTCC
GACCGTCCGGAACTGCTGGTCGACCTCAACCTGCCGCAAAACAGCAGTATCCACGAGACCAGGGCGGTGATG
GACCGCCTGGAAGCGACGCTGAAGGACGACGAGGACATCGACCACTGGAGCGCCTACGTCGGCGAAGGCGCG
ATCCGCTTCTACCTGCCGCTGGACCAGCAGTTGCAGAACAACTTCTATGGCCAACTGGTGATCGTCACCAAG
GACTTGGAGGCCCGCGAGCGCGTCGCCGCGCGCCTGCGCGATCGCTTGCGCAAGGACTATGTCGGCATCAGC
ACCTACGTGCAGCCGCTGGAGATGGGGCCGCCGGTGGGACGGCCGATCCAGTACCGGGTCAGCGGACCGCAG
ATCGACAAGGTCCGCGAGTACGCCATGGGCCTGGCCGGCGTGCTCGACGGCAACCCGAACATCGGCGATATC
GTCTACGACTGGAACGAGCCCGGGAAGATGCTCAAGATCGACATCGCCCAGGACAAGGCGCGCCAGCTCGGG
CTGTCCTCCGAGGACGTGGCGCAGATCATGAACAGCGTGGTGACCGGCAGCGCGGTGACCCAGGTGCGCGAC
GACATCTACCTGGTGAACGTCATCGGCCGCGCCGAGGATAGCGAGCGCGGCTCGCTGGAGACCCTGGAGAGC
CTGCAGATCGTCACGCCCAGCGGCACCTCGATCCCGCTCAAGGCGTTCGCCAAGGTCAGCTACGAGCTGGAG
CAGCCGCTGGTGTGGCGCCGCGACCGCAAGCCGACGATCACGGTGAAGGCGTCGCTGCGCGGCGAGATCCAG
CCCACCGACCTGGTCGCCAAGCTGGCGCCGGAGGTCAAGCGCTTCGCCGACGGCCTGCCGGCGAACTACCGG
ATCGAAGTGGGCGGTACGGTGGAGGAGAGCGGCAAGGCCGAGGGCCCGATCGCCAAGGTGGTGCCGCTGATG
CTGTTCCTCATGGCGACCTTCCTGATGATCCAGTTGCAGAGCGTGCAGAAGCTGTTCCTGGTGGCCAGCGTC
GCGCCGCTGGGGCTGATCGGCGTGGTCGCGGCGTTGCTGCCGACCGGCACGCCGATGGGCTTCGTGGCGATC
CTCGGGATTCTCGCGCTGATCGGCATCATCATCCGCAACTCGGTGATCCTGGTGACCCAGATCGATGCCTTC
GAGAAAGACGGCAAGACGCCCTGGGAAGCCGTGCTGGAAGCCACCCACCACCGCACCCGGCCGATCCTGCTG
ACGGCGGCGGCGGCCAGCCTGGGGATGATCCCCATCGCCCGCGAAGTGTTCTGGGGGCCGATGGCCTATGCG
ATGATCGGCGGCATCGTTGCCGCCACGCTGCTCACGCTGATCTTCCTGCCGGCGCTGTACGTCGCCTGGTAC
CGGATTCCGGAACCGGGACGCTGA
Translation:
MKGGFNLSDWALRHQSLVWYLMAVSLVMGVFSYLNLGREEDPSFAIKTMVIQTRWPGATVDDTLEQVTDRIE
KKLEELDSLDYVKSYTRPGESTVFVYLKDTTKAGDIPDIWYQVRKKISDIQGEFPQGIQGPGFNDEFGDVFG
SVYAFTADGLDFRQLRDYVEKVRLDIRSVKDLGKVQMIGAQNEVIYLNFSTRKLAALGLDQRQVVQSLQAQN
AVTPSGVVEAGPERISVRTSGNFRSEKDLQAVNLRVNDRFYRLSDLASISRDFVDPPTSLFRYKGEPAIGLA
VAMKEGGNILEFGEALNARMQEITGELPVGVGVHQVSNQAQVVKKAVGGFTQALFEAVVIVLIVSFVSLGLR
AGLVVACSIPLVLAMVFVFMEYTDITMQRVSLGALIIALGLLVDDAMITVEMMITRLELGDSLHDSATYAYT
STAFPMLTGTLVTVAGFVPIGLNASSAGEYTFTLFAVIAVALLLSWIVAVLFAPVIAVHILPKTLKHKSEQK
KGRIAERFDSLLHLAMRRRWTTIFLTALLFGVSLFLMKFVQHQFFPSSDRPELLVDLNLPQNSSIHETRAVM
DRLEATLKDDEDIDHWSAYVGEGAIRFYLPLDQQLQNNFYGQLVIVTKDLEARERVAARLRDRLRKDYVGIS
TYVQPLEMGPPVGRPIQYRVSGPQIDKVREYAMGLAGVLDGNPNIGDIVYDWNEPGKMLKIDIAQDKARQLG
LSSEDVAQIMNSVVTGSAVTQVRDDIYLVNVIGRAEDSERGSLETLESLQIVTPSGTSIPLKAFAKVSYELE
QPLVWRRDRKPTITVKASLRGEIQPTDLVAKLAPEVKRFADGLPANYRIEVGGTVEESGKAEGPIAKVVPLM
LFLMATFLMIQLQSVQKLFLVASVAPLGLIGVVAALLPTGTPMGFVAILGILALIGIIIRNSVILVTQIDAF
EKDGKTPWEAVLEATHHRTRPILLTAAAASLGMIPIAREVFWGPMAYAMIGGIVAATLLTLIFLPALYVAWY
RIPEPGR*
AnnotationID:81GeneID:71
AnnotatorUID: diggins
Modification Date/Time: 2005-03-23 07:07:05
Gene Name:
Confidence Code:3
GeneProduct:putative RND efflux transporter
Cell Localization:()
Synonyms:PA0158
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative AcrB/AcrD/AcrF multidrug efflux protein
Functional Category Confidence Code:5
COGs:COG0841,pfam00873,COG3696
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58617.1| (AE016874) AcrB/AcrD/AcrF family protein [Pseudomonas syringae pv.
            tomato str. DC3000]
          Length = 1033

 Score = 1578 bits (4087), Expect = 0.0
 Identities = 786/1013 (77%), Positives = 898/1013 (88%), Gaps = 1/1013 (0%)

>gb|AAN70738.1|AE016717_6 (AE016793) RND efflux transporter [Pseudomonas putida KT2440]
          Length = 1017

 Score = 1534 bits (3971), Expect = 0.0
 Identities = 761/1013 (75%), Positives = 881/1013 (86%)

>gb|AAN34086.1|AE014585_1 (AE014585) AcrB/AcrD/AcrF multidrug efflux protein [Brucella suis
            1330]
          Length = 1024

 Score = 1311 bits (3394), Expect = 0.0
 Identities = 647/1014 (63%), Positives = 811/1014 (79%), Gaps = 2/1014 (0%)






 
Structural Features:
COG0841, AcrB, Cation/multidrug efflux pump [Defense mechanisms]
CD-Length = 1009 residues, 100.0% aligned
Score =  826 bits (2134), Expect = 0.0
Identities = 300/1026 (29%), Positives = 495/1026 (48%), Gaps = 38/1026 (3%)

pfam00873, ACR_tran, AcrB/AcrD/AcrF family.
CD-Length = 1017 residues,  99.7% aligned
Score =  475 bits (1223), Expect = 2e-134
Identities = 284/1035 (27%), Positives = 489/1035 (47%), Gaps = 57/1035 (5%)

COG3696, COG3696, Putative silver efflux pump 
[Inorganic ion transport and metabolism]
CD-Length = 1027 residues,  98.5% aligned
Score =  420 bits (1081), Expect = 5e-118
Identities = 275/1037 (26%), Positives = 470/1037 (45%), Gaps = 63/1037 (6%)







   
Genomic Context:






   
Comment:


















    ReferenceID:145




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17287




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33530




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:71_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:71
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
144
                           PA0158_tr
                           translation of PA0158
                           1015
                           99.80
                           99.99
                           0
                           5096.0
     

     
     
13450
                           gb|AAG03548.1|AE004453_11
                           (AE004453) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
                           1015
                           99.80
                           99.99
                           0
                           5096.0
     

     
     
13451
                           gb|AAO58617.1|
                           (AE016874) AcrB/AcrD/AcrF family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           1034
                           76.01
                           86.84
                           20
                           4087.0
     

     
     
13452
                           gb|AAN70738.1|AE016717_6
                           (AE016793) RND efflux transporter [Pseudomonas putida KT2440]
                           1017
                           74.82
                           86.72
                           2
                           3971.0
     

     
     
13453
                           gb|AAO56780.1|
                           (AE016867) AcrB/AcrD/AcrF family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           1015
                           70.44
                           83.25
                           4
                           3691.0
     

     






      

          
GC ORFID: 43073How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0158Glimmer Score: 

          
Start: 5979721Stop: 5982768

          
Length: 3048


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0158 translation of PA0158Homolog Bit Score 1967.0

          
Other Homologs:  psyr_15may02_Scaffold11_revised_gene1933 (ORF: BLASTX 1414.0), t_PA3676 (ORF: BLASTX 899.0), t_PA0158 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGGGCGGTTTCAACCTGTCGGACTGGGCCCTGCGCCACCAGTCCCTGGTCTGGTACCTGATGGCGGTA 
TCGCTGGTGATGGGCGTGTTCTCCTACCTCAACCTGGGGCGCGAGGAGGATCCCTCGTTCGCCATCAAGACC 
ATGGTCATCCAGACCCGCTGGCCGGGCGCCACGGTGGACGACACCCTGGAGCAGGTCACCGACCGCATCGAG 
AAGAAGCTCGAGGAGCTGGACTCGCTGGATTACGTGAAGAGCTACACGCGACCCGGCGAATCGACGGTCTTC 
GTCTACCTCAAGGACACCACCAAGGCCGGCGACATCCCGGATATCTGGTACCAGGTGCGCAAGAAGATCTCC 
GACATCCAGGGCGAATTCCCCCAAGGCATCCAGGGACCGGGTTTCAACGACGAGTTCGGCGACGTGTTCGGC 
AGCGTCTACGCCTTCACCGCCGACGGCCTGGACTTCCGCCAGCTGCGCGACTACGTGGAGAAGGTGCGCCTG 
GACATCCGCTCGGTGAAAGACCTGGGCAAGGTGCAGATGATCGGTGCGCAGAACGAGGTCATCTACCTCAAC 
TTCTCCACCCGCAAGCTGGCCGCCCTCGGTCTCGACCAGCGCCAGGTCGTGCAGAGCCTGCAAGCGCAGAAC 
GCGGTGACCCCGTCCGGCGTGGTCGAGGCCGGCCCCGAGCGCATCTCGGTGCGCACCTCCGGCAACTTCCGT 
TCGGAAAAGGACCTGCAGGCGGTCAACCTGCGGGTCAACGATCGTTTCTACCGGCTGTCCGACCTAGCCAGC 
ATCAGCCGCGATTTCGTCGACCCGCCGACCTCGCTGTTCCGCTACAAGGGCGAGCCGGCCATCGGCCTGGCG 
GTGGCGATGAAGGAGGGCGGCAATATCCTCGAGTTCGGCGAGGCGCTCAATGCGCGCATGCAGGAGATCACC 
GGCGAACTGCCGGTCGGGGTCGGCGTGCACCAGGTGTCGAACCAGGCCCAGGTGGTGAAGAAGGCGGTCGGC 
GGTTTCACCCAGGCGCTGTTCGAGGCGGTGGTGATCGTCCTCATCGTCAGCTTCGTCAGCCTCGGCCTGCGC 
GCCGGGCTGGTGGTGGCCTGCTCGATCCCGCTGGTGCTGGCGATGGTCTTCGTGTTCATGGAATACACCGAC 
ATCACCATGCAGCGGGTTTCCCTCGGCGCGCTGATCATCGCCCTCGGCCTGCTGGTGGACGATGCCATGATC 
ACCGTGGAGATGATGATCACGCGCCTCGAACTGGGCGACTCGCTGCACGACTCGGCGACCTACGCCTACACC 
TCGACGGCCTTCCCGATGCTCACCGGGACCCTGGTGACGGTGGCCGGCTTCGTGCCCATCGGGCTCAACGCC 
AGCTCCGCCGGCGAGTACACCTTCACCCTGTTCGCGGTGATCGCCGTGGCCCTGCTGCTGTCGTGGATCGTC 
GCGGTGCTGTTCGCCCCGGTGATCGCCGTGCACATCCTGCCGAAGACTCTCAAGCACAAGTCGGAGCAGAAG 
AAGGGGCGCATCGCCGAGCGTTTCGACAGCCTGCTGCACCTGGCGATGCGCCGGCGCTGGACGACCATCTTC 
CTCACCGCGCTGCTGTTCGGCGTGTCGCTGTTCCTGATGAAGTTCGTCCAGCACCAGTTCTTCCCGTCTTCC 
GACCGTCCGGAACTGCTGGTCGACCTCAACCTGCCGCAAAACAGCAGTATCCACGAGACCAGGGCGGTGATG 
GACCGCCTGGAAGCGACGCTGAAGGACGACGAGGACATCGACCACTGGAGCGCCTACGTCGGCGAAGGCGCG 
ATCCGCTTCTACCTGCCGCTGGACCAGCAGTTGCAGAACAACTTCTATGGCCAACTGGTGATCGTCACCAAG 
GACTTGGAGGCCCGCGAGCGCGTCGCCGCGCGCCTGCGCGATCGCTTGCGCAAGGACTATGTCGGCATCAGC 
ACCTACGTGCAGCCGCTGGAGATGGGGCCGCCGGTGGGACGGCCGATCCAGTACCGGGTCAGCGGACCGCAG 
ATCGACAAGGTCCGCGAGTACGCCATGGGCCTGGCCGGCGTGCTCGACGGCAACCCGAACATCGGCGATATC 
GTCTACGACTGGAACGAGCCCGGGAAGATGCTCAAGATCGACATCGCCCAGGACAAGGCGCGCCAGCTCGGG 
CTGTCCTCCGAGGACGTGGCGCAGATCATGAACAGCGTGGTGACCGGCAGCGCGGTGACCCAGGTGCGCGAC 
GACATCTACCTGGTGAACGTCATCGGCCGCGCCGAGGATAGCGAGCGCGGCTCGCTGGAGACCCTGGAGAGC 
CTGCAGATCGTCACGCCCAGCGGCACCTCGATCCCGCTCAAGGCGTTCGCCAAGGTCAGCTACGAGCTGGAG 
CAGCCGCTGGTGTGGCGCCGCGACCGCAAGCCGACGATCACGGTGAAGGCGTCGCTGCGCGGCGAGATCCAG 
CCCACCGACCTGGTCGCCAAGCTGGCGCCGGAGGTCAAGCGCTTCGCCGACGGCCTGCCGGCGAACTACCGG 
ATCGAAGTGGGCGGTACGGTGGAGGAGAGCGGCAAGGCCGAGGGCCCGATCGCCAAGGTGGTGCCGCTGATG 
CTGTTCCTCATGGCGACCTTCCTGATGATCCAGTTGCAGAGCGTGCAGAAGCTGTTCCTGGTGGCCAGCGTC 
GCGCCGCTGGGGCTGATCGGCGTGGTCGCGGCGTTGCTGCCGACCGGCACGCCGATGGGCTTCGTGGCGATC 
CTCGGGATTCTCGCGCTGATCGGCATCATCATCCGCAACTCGGTGATCCTGGTGACCCAGATCGATGCCTTC 
GAGAAAGACGGCAAGACGCCCTGGGAAGCCGTGCTGGAAGCCACCCACCACCGCACCCGGCCGATCCTGCTG 
ACGGCGGCGGCGGCCAGCCTGGGGATGATCCCCATCGCCCGCGAAGTGTTCTGGGGGCCGATGGCCTATGCG 
ATGATCGGCGGCATCGTTGCCGCCACGCTGCTCACGCTGATCTTCCTGCCGGCGCTGTACGTCGCCTGGTAC 
CGGATTCCGGAACCGGGACGCTGA