ViewGene
Locus Name:
GeneID:
Location:

GeneID:709
TrivialName: PA14_17960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17960
PAO1 Ortholog Locus:PA3582
Sequence Length:1518
Protein Length:505
Start: 1544325
Stop: 1542808
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06970.1|AE004779_2 (AE004779) glycerol kinase [Pseudomonas aeruginosa PAO1]
Identities = 504/505 (99%), Positives = 504/505 (99%)
Sequence:
ATGACCGACAAGCACAACAAGAAATACGTCGTCGCCCTGGACCAGGGCACCACCAGCTCCCGCGCCATCGTC
TTCGACCGCGATGCCAACGTGGTCAGCCAGGCCCAGCGCGAGTTCGCCCAGTTCTATCCGCAGGCCGGCTGG
GTCGAACACGATCCGATGGAAATCTGGGCCACGCAGAGTTCGACCCTGGTCGAGGCCCTCGCCCAAGCCAGC
ATCGAGCACGACCAGGTGGCCGCCATCGGCATCACCAACCAGCGCGAGACCACGGTGGTCTGGGACCGTCAC
AGCGGTCGGCCGATCCACAACGCCATCGTCTGGCAGTGCCGGCGCAGCGCGGCGATCTGCGCGCAGCTCAAG
CGCGACGGGCTGGAAGACTACATCCGCGAAACCACCGGTCTGGTCACCGACCCGTACTTTTCCGGGACCAaG
CTGAAGTGGATCCTCGACAACGTCGAaggCGCCCGCGAACGCGCGCGCAACGGCGACCTGTTGTTCGGCACC
ATCGACACCTGGCTGATCTGGAAGCTCACCGAAGGCAAGGTCCACGTCACCGACTACACCAATGCCTCGCGG
ACCATGCTGTTCAACATCCACAGCCTCGACTGGGACGCACGGATGCTCGAGGTGCTCGACATTCCCCGCTCG
ATGCTGCCCGAGGTGCGCAACTCTTCGGAGGTCTACGGCAACGCCCGGATCGGCGGGGTCGGCGGCGGCGAG
CTGCCGATCGCCGGGATCGCCGGCGACCAGCAGGCCGCCCTGTTCGGCCAGATGTGCGTGGAACCGGGCCAG
GCGAAGAACACCTACGGCACCGGCTGCTTCCTGCTGATGCACACCGGCGACAAGGCGGTGAAGTCCACCCAT
GGCCTGCTCACCACCATCGCCTGCGGCCCGCGCGGCGAGGTCGGCTACGCCCTGGAGGGCGCGGTGTTCAAC
GGCGGCTCCACGGTGCAGTGGTTGCGCGACGAACTGAAGGTGATCAACGACTCCTTCGATTCCGAATACTTC
GCCACCAAGGTCAAGGACAGCAACGGCGTCTACCTGGTACCGGCCTTCACCGGACTCGGCGCGCCCTATTGG
GACCCCTATGCGCGCGGCGCAGTGTTCGGCCTGACCCGTGGAGTCAAGGCCGACCACCTGATCCGCGCCACC
CTGGAATCCATCGCCTACCAGACCCGCGACGTGCTCGACGCCATGCAGCGCGATGCCGGCGAACGCCTGCGC
GCTCTGCGCGTCGACGGCGGGGCGGTGGCCAACAACTTCCTCATGCAGTTCCAGGCCGACATCCTCGGCACC
CGCGTGGAGCGCCCGGTGATGCGCGAGACCACCGCCCTCGGCGCCGCCTACCTGGCCGGCCTGGCCTGCGGC
TTCTGGAGCAGCCTCGACGAGCTGAAGAGCAAGGCGGTGATCGAGCGGGTGTTCGAGCCGGAGTGCGACGAG
CCCCGGCGCGAAAAGCTCTATGCCGGCTGGAAAAAAGCGGTGGAGCGCACTCGCGGCTGGGACGACGGCGAA
CTGTGA
Translation:
MTDKHNKKYVVALDQGTTSSRAIVFDRDANVVSQAQREFAQFYPQAGWVEHDPMEIWATQSSTLVEALAQAS
IEHDQVAAIGITNQRETTVVWDRHSGRPIHNAIVWQCRRSAAICAQLKRDGLEDYIRETTGLVTDPYFSGTK
LKWILDNVEGARERARNGDLLFGTIDTWLIWKLTEGKVHVTDYTNASRTMLFNIHSLDWDARMLEVLDIPRS
MLPEVRNSSEVYGNARIGGVGGGELPIAGIAGDQQAALFGQMCVEPGQAKNTYGTGCFLLMHTGDKAVKSTH
GLLTTIACGPRGEVGYALEGAVFNGGSTVQWLRDELKVINDSFDSEYFATKVKDSNGVYLVPAFTGLGAPYW
DPYARGAVFGLTRGVKADHLIRATLESIAYQTRDVLDAMQRDAGERLRALRVDGGAVANNFLMQFQADILGT
RVERPVMRETTALGAAYLAGLACGFWSSLDELKSKAVIERVFEPECDEPRREKLYAGWKKAVERTRGWDDGE
L*
AnnotationID:718GeneID:709
AnnotatorUID: diggins
Modification Date/Time: 2005-07-04 19:07:21
Gene Name:glpK
Confidence Code:1
GeneProduct:glycerol kinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0554,KOG2517,COG1070
Secondary Functional Category(ies):energy metabolism
EC Number:2.7.1.30
Status:ACTIVE
Pathway:
Glycerolipid metabolism.
Glycerol utilization; rate-limiting step.
Homology:
>gb|AAN66700.1|AE016298_4 (AE016778) glycerol kinase [Pseudomonas putida KT2440]
          Length = 499

 Score =  862 bits (2226), Expect = 0.0
 Identities = 415/504 (82%), Positives = 457/504 (90%), Gaps = 6/504 (1%)

>gb|AAB03058.1| (L19201) glycerol kinase [Escherichia coli]
          Length = 502

 Score =  811 bits (2095), Expect = 0.0
 Identities = 383/496 (77%), Positives = 440/496 (88%)
Structural Features:
COG0554, GlpK, Glycerol kinase [Energy production and conversion]

KOG2517, Ribulose kinase and related carbohydrate kinases 
[Carbohydrate transport and metabolism]

COG1070, XylB, Sugar (pentulose and hexulose) kinases 
[Carbohydrate transport and met
Genomic Context:






   
Comment:


















    ReferenceID:1421




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18563




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31960




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:709_cdd.html


    		




 
URL:

















    ReferenceID:34158




  
Author/Investigator(s):
        Schweizer HP, Jump R, Po C.





  
Title:
        Structure and gene-polypeptide relationships of the region encoding glycerol diffusion facilitator (glpF) and glycerol kinase (glpK) of Pseudomonas aeruginosa.




  
PubMed:
        9141691


MedLine:
        




    
Source:
Microbiology. 1997 Apr;143 ( Pt 4):1287-97.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9141691









           
Homologs By Global Alignment 

           

       



     
Gene ID:709
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1220
                           PA3582_tr
                           translation of PA3582
                           505
                           99.80
                           99.80
                           0
                           2650.0
     

     
     
46581
                           gb|AAG06970.1|AE004779_2
                           (AE004779) glycerol kinase [Pseudomonas aeruginosa PAO1]
                           505
                           99.80
                           99.80
                           0
                           2650.0
     

     
     
46582
                           gb|AAB57804.1|
                           (U49666) glycerol kinase [Pseudomonas aeruginosa]
                           505
                           99.20
                           99.20
                           0
                           2626.0
     

     
     
46583
                           gb|AAN66700.1|AE016298_4
                           (AE016778) glycerol kinase [Pseudomonas putida KT2440]
                           505
                           82.17
                           90.49
                           6
                           2229.0
     

     
     
46584
                           gb|AAO57624.1|
                           (AE016871) glycerol kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           507
                           80.47
                           90.33
                           8
                           2203.0
     

     






      

          
GC ORFID: 43711How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3582Glimmer Score: 

          
Start: 3409611Stop: 3411128

          
Length: 1518


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3582 translation of PA3582Homolog Bit Score 1025.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6126 (ORF: BLASTX 853.0), t_PA3579 (ORF: BLASTX 546.0), t_PA3582 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGACAAGCACAACAAGAAATACGTCGTCGCCCTGGACCAGGGCACCACCAGCTCCCGCGCCATCGTC 
TTCGACCGCGATGCCAACGTGGTCAGCCAGGCCCAGCGCGAGTTCGCCCAGTTCTATCCGCAGGCCGGCTGG 
GTCGAACACGATCCGATGGAAATCTGGGCCACGCAGAGTTCGACCCTGGTCGAGGCCCTCGCCCAAGCCAGC 
ATCGAGCACGACCAGGTGGCCGCCATCGGCATCACCAACCAGCGCGAGACCACGGTGGTCTGGGACCGTCAC 
AGCGGTCGGCCGATCCACAACGCCATCGTCTGGCAGTGCCGGCGCAGCGCGGCGATCTGCGCGCAGCTCAAG 
CGCGACGGGCTGGAAGACTACATCCGCGAAACCACCGGTCTGGTCACCGACCCGTACTTTTCCGGGACCAaG 
CTGAAGTGGATCCTCGACAACGTCGAaggCGCCCGCGAACGCGCGCGCAACGGCGACCTGTTGTTCGGCACC 
ATCGACACCTGGCTGATCTGGAAGCTCACCGAAGGCAAGGTCCACGTCACCGACTACACCAATGCCTCGCGG 
ACCATGCTGTTCAACATCCACAGCCTCGACTGGGACGCACGGATGCTCGAGGTGCTCGACATTCCCCGCTCG 
ATGCTGCCCGAGGTGCGCAACTCTTCGGAGGTCTACGGCAACGCCCGGATCGGCGGGGTCGGCGGCGGCGAG 
CTGCCGATCGCCGGGATCGCCGGCGACCAGCAGGCCGCCCTGTTCGGCCAGATGTGCGTGGAACCGGGCCAG 
GCGAAGAACACCTACGGCACCGGCTGCTTCCTGCTGATGCACACCGGCGACAAGGCGGTGAAGTCCACCCAT 
GGCCTGCTCACCACCATCGCCTGCGGCCCGCGCGGCGAGGTCGGCTACGCCCTGGAGGGCGCGGTGTTCAAC 
GGCGGCTCCACGGTGCAGTGGTTGCGCGACGAACTGAAGGTGATCAACGACTCCTTCGATTCCGAATACTTC 
GCCACCAAGGTCAAGGACAGCAACGGCGTCTACCTGGTACCGGCCTTCACCGGACTCGGCGCGCCCTATTGG 
GACCCCTATGCGCGCGGCGCAGTGTTCGGCCTGACCCGTGGAGTCAAGGCCGACCACCTGATCCGCGCCACC 
CTGGAATCCATCGCCTACCAGACCCGCGACGTGCTCGACGCCATGCAGCGCGATGCCGGCGAACGCCTGCGC 
GCTCTGCGCGTCGACGGCGGGGCGGTGGCCAACAACTTCCTCATGCAGTTCCAGGCCGACATCCTCGGCACC 
CGCGTGGAGCGCCCGGTGATGCGCGAGACCACCGCCCTCGGCGCCGCCTACCTGGCCGGCCTGGCCTGCGGC 
TTCTGGAGCAGCCTCGACGAGCTGAAGAGCAAGGCGGTGATCGAGCGGGTGTTCGAGCCGGAGTGCGACGAG 
CCCCGGCGCGAAAAGCTCTATGCCGGCTGGAAAAAAGCGGTGGAGCGCACTCGCGGCTGGGACGACGGCGAA 
CTGTGA