ViewGene
Locus Name:
GeneID:
Location:

GeneID:698
TrivialName: PA14_57010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57010
PAO1 Ortholog Locus:PA4385
Sequence Length:1644
Protein Length:547
Start: 5080678
Stop: 5079035
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07773.1|AE004854_9 (AE004854) GroEL protein [Pseudomonas aeruginosa PAO1]
Identities = 547/547 (100%), Positives = 547/547 (100%)
Sequence:
ATGGCTGCCAAAGAAGTTAAGTTCGGCGATTCCGCTCGCAAGAAAATGCTGGTCGGCGTGAACGTGCTGGCC
GATGCCGTCAAGGCCACCCTCGGCCCGAAAGGCCGCAACGTGGTTCTGGACAAGTCGTTCGGCGCTCCGACC
ATCACCAAGGATGGCGTTTCCGTCGCCAAGGAAATCGAGCTGAAAGACAAGTTCGAGAACATGGGCGCGCAA
CTGGTGAAAGACGTTGCCTCCAAGGCCAACGATGCTGCCGGTGACGGCACCACCACCGCGACCGTCCTGGCC
CAGGCCATCGTCAACGAAGGCCTGAAGGCCGTTGCCGCCGGCATGAACCCGATGGACCTGAAGCGTGGCATC
GACAAGGCCACCGTGGCCATCGTCGCCCAGCTGAAAGAGCTGGCCAAGCCCTGCGCCGACACCAAGGCCATC
GCCCAGGTAGGCACCATCTCCGCCAACTCCGACGAGTCCATCGGCCAGATCATTGCCGAAGCCATGGAAAAA
GTCGGTAAAGAAGGCGTGATCACCGTCGAGGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTCGAAGGC
ATGCAGTTCGATCGCGGCTACCTGTCCCCCTACTTCGTGAACAAGCCGGACACCATGGCTGCCGAGCTGGAT
AGCCCGCTGCTGCTGCTGGTCGACAAGAAGATCTCCAACATCCGCGAAATGCTGCCGGTGCTGGAAGCCGTC
GCCAAGGCCGGCCGTCCGCTGCTGATCGTCGCCGAGGACGTCGAGGGCGAAGCCCTGGCCACCCTGGTGGTG
AACAACATGCGTGGCATCGTCAAGGTCGCGGCCGTCAAGGCTCCGGGCTTCGGCGACCGCCGCAAGGCCATG
CTGCAGGACATCGCCATCCTCACCGGCGGTACCGTGATCAGCGAAGAAGTCGGCCTGAGCCTGGAAGGCGCT
ACCCTGGAGCACCTGGGCAACGCCAAGCGCGTCGTGATCAACAAGGAAAACACCACCATCATCGATGGCGCC
GGTGTGCAGGCTGATATCGAAGCCCGCGTCCTGCAGATCCGCAAGCAGATCGAGGAAACCACTTCCGACTAC
GACCGCGAGAAGCTGCAAGAGCGCCTGGCCAAGCTGGCCGGCGGTGTTGCCGTGATCAAGGTAGGCGCTGCC
ACCGAAGTCGAGATGAAAGAGAAGAAAGCCCGCGTCGAAGACGCCCTGCACGCTACCCGTGCGGCGGTGGAA
GAGGGCGTGGTTCCCGGCGGCGGCGTAGCCCTGGTTCGTGCCCTGCAAGCCATCGAAGGCCTGAAGGGTGAC
AACGAGGAGCAGAACGTCGGTATCGCCCTGCTGCGTCGCGCCGTCGAATCGCCGCTGCGCCAGATCGTGGCC
AACGCCGGCGACGAGCCGAGCGTAGTGGTCGACAAGGTCAAGCAGGGTTCCGGCAACTACGGCTTCAACGCT
GCTACCGGCGTGTACGGCGACATGATCGAGATGGGCATCCTGGACCCGGCCAAGGTCACTCGTTCCGCTCTG
CAGGCTGCGGCCTCCATCGGCGGTCTGATGATCACCACCGAAGCCATGGTTGCCGAGATCGTGGAAGACAAG
CCGGCCATGGGCGGCATGCCTGACATGGGCGGCATGGGCGGCATGGGCGGCATGATGTAA
Translation:
MAAKEVKFGDSARKKMLVGVNVLADAVKATLGPKGRNVVLDKSFGAPTITKDGVSVAKEIELKDKFENMGAQ
LVKDVASKANDAAGDGTTTATVLAQAIVNEGLKAVAAGMNPMDLKRGIDKATVAIVAQLKELAKPCADTKAI
AQVGTISANSDESIGQIIAEAMEKVGKEGVITVEEGSGLENELSVVEGMQFDRGYLSPYFVNKPDTMAAELD
SPLLLLVDKKISNIREMLPVLEAVAKAGRPLLIVAEDVEGEALATLVVNNMRGIVKVAAVKAPGFGDRRKAM
LQDIAILTGGTVISEEVGLSLEGATLEHLGNAKRVVINKENTTIIDGAGVQADIEARVLQIRKQIEETTSDY
DREKLQERLAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVPGGGVALVRALQAIEGLKGD
NEEQNVGIALLRRAVESPLRQIVANAGDEPSVVVDKVKQGSGNYGFNAATGVYGDMIEMGILDPAKVTRSAL
QAAASIGGLMITTEAMVAEIVEDKPAMGGMPDMGGMGGMGGMM*
AnnotationID:707GeneID:698
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 12:12:50
Gene Name:groEL
Confidence Code:1
GeneProduct: GroEL protein
Cell Localization:()
Synonyms:mopA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:mitochondrial chaperonin, Cpn60/Hsp60p
Functional Category Confidence Code:5
COGs:KOG0356,COG0459,pfam00118
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA74154.1| (Y13828) Hsp60 protein [Pseudomonas stutzeri]
          Length = 552

 Score =  963 bits (2489), Expect = 0.0
 Identities = 506/546 (92%), Positives = 524/546 (95%), Gaps = 1/546 (0%)

>gb|AAL25964.1| (AY057439) GroEL [Azotobacter vinelandii]
          Length = 546

 Score =  959 bits (2478), Expect = 0.0
 Identities = 507/547 (92%), Positives = 526/547 (96%), Gaps = 1/547 (0%)

>gb|AAN66984.1|AE016326_10 (AE016779) chaperonin, 60 kDa [Pseudomonas putida KT2440]
          Length = 546

 Score =  955 bits (2468), Expect = 0.0
 Identities = 502/547 (91%), Positives = 523/547 (95%), Gaps = 1/547 (0%)







 
Structural Features:
KOG0356, Mitochondrial chaperonin, Cpn60/Hsp60p 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 550 residues,  97.5% aligned
Score =  568 bits (1464), Expect = 2e-162
Identities = 300/539 (55%), Positives = 409/539 (75%), Gaps = 5/539 (0%)

COG0459, GroL, Chaperonin GroEL (HSP60 family) 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 524 residues, 100.0% aligned
Score =  487 bits (1256), Expect = 2e-138
Identities = 269/550 (48%), Positives = 347/550 (63%), Gaps = 42/550 (7%)

pfam00118, Cpn60_TCP1, TCP-1/cpn60 chaperonin family.
CD-Length = 486 residues,  99.8% aligned
Score =  356 bits (915), Expect = 9e-99
Identities = 189/510 (37%), Positives = 284/510 (55%), Gaps = 33/510 (6%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1399




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17142




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:698_cdd.html


    		




 
URL:

















    ReferenceID:18541




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32700




  
Author/Investigator(s):
        Fujita M, Amemura A, Aramaki H.





  
Title:
        Transcription of the groESL operon in Pseudomonas aeruginosa PAO1.




  
PubMed:
        9673028


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1998 Jun 15;163(2):237-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9673028

















    ReferenceID:32701




  
Author/Investigator(s):
        Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock RE, Levesque RC.





  
Title:
        Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.




  
PubMed:
        8581173


MedLine:
        




    
Source:
Microbiology. 1996 Jan;142 ( Pt 1):79-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8581173

















    ReferenceID:32702




  
Author/Investigator(s):
        Jensen P, Fomsgaard A, Hoiby N, Hindersson P.





  
Title:
        Cloning and nucleotide sequence comparison of the groE operon of Pseudomonas aeruginosa and Burkholderia cepacia.




  
PubMed:
        7538307


MedLine:
        




    
Source:
APMIS. 1995 Feb;103(2):113-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7538307

















    ReferenceID:32703




  
Author/Investigator(s):
        Jensen P, Fomsgaard A, Shand G, Hindersson P, Hoiby N.





  
Title:
        Antigenic analysis of Pseudomonas aeruginosa and Pseudomonas cepacia GroEL proteins and demonstration of a lipopolysaccharide-associated GroEL fraction in P. aeruginosa.




  
PubMed:
        7692893


MedLine:
        




    
Source:
APMIS. 1993 Aug;101(8):621-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7692893

















    ReferenceID:32704




  
Author/Investigator(s):
        Allan B, Linseman M, MacDonald LA, Lam JS, Kropinski AM.





  
Title:
        Heat shock response of Pseudomonas aeruginosa.




  
PubMed:
        3136146


MedLine:
        




    
Source:
J Bacteriol. 1988 Aug;170(8):3668-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3136146









           
Homologs By Global Alignment 

           

       



     
Gene ID:698
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1182
                           PA4385_tr
                           translation of PA4385
                           547
                           99.99
                           99.99
                           0
                           2672.0
     

     
     
43952
                           gb|AAG07773.1|AE004854_9
                           (AE004854) GroEL protein [Pseudomonas aeruginosa PAO1]
                           547
                           99.99
                           99.99
                           0
                           2672.0
     

     
     
43953
                           gb|AAA53369.1|
                           (U17072) GroEL [Pseudomonas aeruginosa]
                           547
                           99.81
                           99.81
                           0
                           2668.0
     

     
     
43954
                           gb|AAB34346.1|
                           (S77424) GroEL; Hsp60-65 [Pseudomonas aeruginosa]
                           547
                           99.08
                           99.45
                           0
                           2648.0
     

     
     
43955
                           gb|AAA25830.1|
                           (M63957) heat shock protein [Pseudomonas aeruginosa]
                           547
                           98.17
                           98.53
                           0
                           2616.0
     

     






      

          
GC ORFID: 43700How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4385Glimmer Score: 

          
Start: 4344354Stop: 4342711

          
Length: 1644


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4385 translation of PA4385Homolog Bit Score 1033.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2056 (ORF: BLASTX 951.0), t_PA4385 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTGCCAAAGAAGTTAAGTTCGGCGATTCCGCTCGCAAGAAAATGCTGGTCGGCGTGAACGTGCTGGCC 
GATGCCGTCAAGGCCACCCTCGGCCCGAAAGGCCGCAACGTGGTTCTGGACAAGTCGTTCGGCGCTCCGACC 
ATCACCAAGGATGGCGTTTCCGTCGCCAAGGAAATCGAGCTGAAAGACAAGTTCGAGAACATGGGCGCGCAA 
CTGGTGAAAGACGTTGCCTCCAAGGCCAACGATGCTGCCGGTGACGGCACCACCACCGCGACCGTCCTGGCC 
CAGGCCATCGTCAACGAAGGCCTGAAGGCCGTTGCCGCCGGCATGAACCCGATGGACCTGAAGCGTGGCATC 
GACAAGGCCACCGTGGCCATCGTCGCCCAGCTGAAAGAGCTGGCCAAGCCCTGCGCCGACACCAAGGCCATC 
GCCCAGGTAGGCACCATCTCCGCCAACTCCGACGAGTCCATCGGCCAGATCATTGCCGAAGCCATGGAAAAA 
GTCGGTAAAGAAGGCGTGATCACCGTCGAGGAAGGCTCGGGCCTGGAAAACGAACTGTCCGTCGTCGAAGGC 
ATGCAGTTCGATCGCGGCTACCTGTCCCCCTACTTCGTGAACAAGCCGGACACCATGGCTGCCGAGCTGGAT 
AGCCCGCTGCTGCTGCTGGTCGACAAGAAGATCTCCAACATCCGCGAAATGCTGCCGGTGCTGGAAGCCGTC 
GCCAAGGCCGGCCGTCCGCTGCTGATCGTCGCCGAGGACGTCGAGGGCGAAGCCCTGGCCACCCTGGTGGTG 
AACAACATGCGTGGCATCGTCAAGGTCGCGGCCGTCAAGGCTCCGGGCTTCGGCGACCGCCGCAAGGCCATG 
CTGCAGGACATCGCCATCCTCACCGGCGGTACCGTGATCAGCGAAGAAGTCGGCCTGAGCCTGGAAGGCGCT 
ACCCTGGAGCACCTGGGCAACGCCAAGCGCGTCGTGATCAACAAGGAAAACACCACCATCATCGATGGCGCC 
GGTGTGCAGGCTGATATCGAAGCCCGCGTCCTGCAGATCCGCAAGCAGATCGAGGAAACCACTTCCGACTAC 
GACCGCGAGAAGCTGCAAGAGCGCCTGGCCAAGCTGGCCGGCGGTGTTGCCGTGATCAAGGTAGGCGCTGCC 
ACCGAAGTCGAGATGAAAGAGAAGAAAGCCCGCGTCGAAGACGCCCTGCACGCTACCCGTGCGGCGGTGGAA 
GAGGGCGTGGTTCCCGGCGGCGGCGTAGCCCTGGTTCGTGCCCTGCAAGCCATCGAAGGCCTGAAGGGTGAC 
AACGAGGAGCAGAACGTCGGTATCGCCCTGCTGCGTCGCGCCGTCGAATCGCCGCTGCGCCAGATCGTGGCC 
AACGCCGGCGACGAGCCGAGCGTAGTGGTCGACAAGGTCAAGCAGGGTTCCGGCAACTACGGCTTCAACGCT 
GCTACCGGCGTGTACGGCGACATGATCGAGATGGGCATCCTGGACCCGGCCAAGGTCACTCGTTCCGCTCTG 
CAGGCTGCGGCCTCCATCGGCGGTCTGATGATCACCACCGAAGCCATGGTTGCCGAGATCGTGGAAGACAAG 
CCGGCCATGGGCGGCATGCCTGACATGGGCGGCATGGGCGGCATGGGCGGCATGATGTAA