ViewGene
Locus Name:
GeneID:
Location:

GeneID:695
TrivialName: PA14_60250
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60250
PAO1 Ortholog Locus:PA4546
Sequence Length:1593
Protein Length:530
Start: 5365845
Stop: 5367437
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAP81268.1| (AY273871) PilS [Pseudomonas aeruginosa]
Identities = 530/530 (100%), Positives = 530/530 (100%)
Sequence:
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGC
CTGACCATCGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCAC
CCTGAACTGTTCCATGTCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCG
TCGCGGCAATTGCTGCCGATCTTCATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCA
GGTGGCGGCGTACCCAGCGGCATCGGCAGCCTGCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGC
GGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCGGCCTGCTCTACCTGACCTTCTTCCTCAGCCTG
AGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGCACCCTGTGCTTCGCCGCCGCGCTG
GTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACGCGCCGAGACGGTCGCC
AACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATAGCCGTCAG
GCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTC
AAGGTGGTGCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTG
CTGATCTTCCTCGAAGACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTTGGCCGC
CTGACCGCCGGCATCGCCCATGAGATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAG
GAGTCAGAGGAACTGGATGCCCCGGACCGACGCCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAAC
CTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCCAGGCCGAACCGCAGCAGCTCGACCTGAAGGAG
TGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGACAGCCAACTGCACCTGCAGCTCGGT
GCCGGCGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAACCTGGTGCAGAACGGT
CTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGGAGAGCGAC
CTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAG
GCACGGATCGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAA
CTCAGCTGA
Translation:
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPP
SRQLLPIFILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSL
SSPDATNHYVQAGGLGTLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQ
AILLANQAALGLLRQDDVQGASLGRHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHV
LIFLEDISQIAQQAQQMKLAGLGRLTAGIAHEIRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMN
LVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRNDSQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNG
LRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVPADKLNNLFEPFFTTESKGTGLGLYLSRELCESNQ
ARIDYRNREEGGGCFRITFAHPRKLS*
AnnotationID:704GeneID:695
AnnotatorUID: diggins
Modification Date/Time: 2005-05-05 06:06:04
Gene Name:pilS
Confidence Code:1
GeneProduct:kinase sensor protein of two component regulatory
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(28) Two-component regulatory systems
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:2.7.3.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54366.1| (AE016858) sensor protein PilS [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 531

 Score =  642 bits (1655), Expect = 0.0
 Identities = 330/532 (62%), Positives = 411/532 (77%), Gaps = 3/532 (0%)








 
Structural Features:






   
Genomic Context:






   
Comment:


















    ReferenceID:14199




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18535




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31662




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:695_cdd.html


    		




 
URL:

















    ReferenceID:32999




  
Author/Investigator(s):
        Boyd JM.





  
Title:
        Localization of the histidine kinase PilS to the poles of Pseudomonas aeruginosa and identification of a localization domain.




  
PubMed:
        10760172


MedLine:
        




    
Source:
Mol Microbiol. 2000 Apr;36(1):153-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10760172

















    ReferenceID:33000




  
Author/Investigator(s):
        Yoshida T, Kim SR, Komano T.





  
Title:
        Twelve pil genes are required for biogenesis of the R64 thin pilus.




  
PubMed:
        10094679


MedLine:
        




    
Source:
J Bacteriol. 1999 Apr;181(7):2038-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10094679

















    ReferenceID:33001




  
Author/Investigator(s):
        Mattick JS, Whitchurch CB, Alm RA.





  
Title:
        The molecular genetics of type-4 fimbriae in Pseudomonas aeruginosa--a review.




  
PubMed:
        8955641


MedLine:
        




    
Source:
Gene. 1996 Nov 7;179(1):147-55.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8955641

















    ReferenceID:33002




  
Author/Investigator(s):
        Boyd JM, Koga T, Lory S.





  
Title:
        Identification and characterization of PilS, an essential regulator of pilin expression in Pseudomonas aeruginosa.




  
PubMed:
        7911557


MedLine:
        




    
Source:
Mol Gen Genet. 1994 Jun 3;243(5):565-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7911557

















    ReferenceID:33003




  
Author/Investigator(s):
        Hobbs M, Collie ES, Free PD, Livingston SP, Mattick JS.





  
Title:
        PilS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa.




  
PubMed:
        8097014


MedLine:
        




    
Source:
Mol Microbiol. 1993 Mar;7(5):669-82.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8097014

















    ReferenceID:33004




  
Author/Investigator(s):
        Boyd JM, Lory S.





  
Title:
        Dual function of PilS during transcriptional activation of the Pseudomonas aeruginosa pilin subunit gene.




  
PubMed:
        8550520


MedLine:
        




    
Source:
J Bacteriol. 1996 Feb;178(3):831-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550520









           
Homologs By Global Alignment 

           

       



     
Gene ID:695
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1179
                           PA4546_tr
                           translation of PA4546
                           530
                           99.99
                           99.99
                           0
                           2673.0
     

     
     
43872
                           gb|AAP81268.1|
                           (AY273871) PilS [Pseudomonas aeruginosa]
                           530
                           99.81
                           99.99
                           0
                           2670.0
     

     
     
43873
                           gb|AAG07934.1|AE004868_5
                           (AE004868) two-component sensor PilS [Pseudomonas aeruginosa PAO1]
                           530
                           99.81
                           99.99
                           0
                           2670.0
     

     
     
43874
                           emb|CAA78138.1|
                           (Z12154) sensor; putative PilR kinase [Pseudomonas aeruginosa]
                           530
                           99.81
                           99.99
                           0
                           2670.0
     

     
     
43875
                           gb|AAA20187.1|
                           (L22436) pilin transcriptional activator [Pseudomonas aeruginosa]
                           530
                           99.43
                           99.99
                           0
                           2666.0
     

     






      

          
GC ORFID: 43697How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4546Glimmer Score: 

          
Start: 4629524Stop: 4631116

          
Length: 1593


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4546 translation of PA4546Homolog Bit Score 1034.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3557 (ORF: BLASTX 629.0), t_PA4546 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGC 
CTGACCATCGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCAC 
CCTGAACTGTTCCATGTCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCG 
TCGCGGCAATTGCTGCCGATCTTCATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCA 
GGTGGCGGCGTACCCAGCGGCATCGGCAGCCTGCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGC 
GGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCGGCCTGCTCTACCTGACCTTCTTCCTCAGCCTG 
AGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGCACCCTGTGCTTCGCCGCCGCGCTG 
GTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACGCGCCGAGACGGTCGCC 
AACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATAGCCGTCAG 
GCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC 
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTC 
AAGGTGGTGCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTG 
CTGATCTTCCTCGAAGACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTTGGCCGC 
CTGACCGCCGGCATCGCCCATGAGATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAG 
GAGTCAGAGGAACTGGATGCCCCGGACCGACGCCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAAC 
CTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCCAGGCCGAACCGCAGCAGCTCGACCTGAAGGAG 
TGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGACAGCCAACTGCACCTGCAGCTCGGT 
GCCGGCGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAACCTGGTGCAGAACGGT 
CTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGGAGAGCGAC 
CTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC 
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAG 
GCACGGATCGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAA 
CTCAGCTGA