ViewGene
Locus Name:
GeneID:
Location:

GeneID:69
TrivialName: PA14_18630
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18630
PAO1 Ortholog Locus:PA3535
Sequence Length:2988
Protein Length:995
Start: 1603228
Stop: 1600241
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06923.1|AE004774_1 (AE004774) probable serine protease [Pseudomonas aeruginosa PAO1]
Identities = 989/995 (99%), Positives = 990/995 (99%)
Sequence:
ATGACCGACGACCACTCCTTCCGCCCTCGCCCCACCTCGTTGTCAGCCGCCCTGCTGCTGGGCGCCTGGATC
GCCCAGCCGGCCACGGCCGCCTATGTCGAAGCCGGTCGGCCCGGCGATCCGGCCAGTTGGCGCTCCGCCGAA
TACCAGCAGGACTGGGGCCTGGAACGGATGCGGGCCGACCAGGCCTATGCCGCCGGCATCGACGGCCAGGGC
GTGAAGATCGGCGAGATGGACTCCGGTTTCGACCCGAGCCATCCGGATACCCCCGCCTCGCGCTACCAGCCG
GTGACGGCCAGCGGCACCTATGTCGACGGCACGCCGTTCAGCGTCAGCGGCGCGATGAACGGCAACAACGAC
TCCCACGGCACCCACGTCGGCGGCACCCTCGGTGCCTCGCGCGACGGCGTCGGCATGCACGGGGTGGCCTAC
GCGGCACAGGTGTACGTCGCCAACACCAACCAGAACGACAGCTTCCTGTTCGGCCCGACGCCCGACCCGAAC
TATTTCAAGGCCGCCTACCAGGCGCTGGCCGACGCCGGGGTGCGGGCGATCAACAACAGTTGGGGCAGCCAG
CCCAAGGACGTCAGCTACGAGACCCTCGACGGCCTGCACGCCGCCTATGCCCAGCACTACGGGCGCTCCACC
TGGCTGGACGCCGCCGCCGGCGTCTCCCGCCAGGGCGTGATCAACGTCTTCAGCGCCGGCAACAGCGGCTAC
GCCAACGCCAGCGTGCGCTCCGCCCTGCCCTACTTCCAGCCGGACCTGGAAGGCCACTGGCTGGCCGTGTCC
GGCCTCGACCAGCAGAACGGCCAGCGCTACAACCGCTGCGGCATCGCCAAGTACTGGTGCATCACCACGCCC
GGCCGCCTGATCAACAGCACCATGCCCGGCGGCGGCTACGCCAACAAGTCCGGTACCTCGATGGCCGCGCCC
CACGCCACCGGCGCGCTGGCCCTGGTCATGCAGCGCTATCCGTACCTGAACAACGAGCAGGCGCTGCAGGTT
CTGCTGACCACCGCCACCCAGCTCGACGGCACGCCGACCGGCGCCCCCACCGACACCGTCGGCTGGGGCGTG
CCGGATCTCGGTCGGGCGATGCATGGGCCTGGACAATTGCTCGGCCGCTTCGAGGCCAACCTCCCGGCCGGC
CTGCGCGACGAATGGAGCAACCCGATTTCCGATAGCGCCCTGCTCCAGCGCCAGGCCGAGGACGCCGCCGAG
CACGCGGCCTGGCAGCGGACGCTGAAGGACAAGGGCTGGGAAAACGGCTTGCCAGCCGGCGCCAGCCAGCAG
GAACGCACCGACTATGCCATCGGCATGGCCCGCGACCAGGCCGCCGCCCAGCGCCAGTACCAGGGCAGCCTG
GTCAAGGCCGGTGCCGGCAGCCTGGTCCTGAGCGGCGACAGCACCTATCGCGGGCCGACCCTGGTCGATGGC
GGGCTGCTCAGCGTCGACGGTTCGCTGCTGTCCGCCGTCGAAGTCAATGCCGGCGGCACCCTCGGCGGCAGC
GGCAGGATCGGCGGCCTGCTGGCGCGCTCCGGCGGCACGGTGGCCGCGGGCAACTCCATCGGCACCCTGGAG
GTCGCCGGGGACCTGCGCTTCGAATCCGGCTCGACCTACGCGGTGGAGCTTTCGGAAAGCGCCAGCGACCGG
ATCGTCGCCAGCGGCAAGGCGAGCATCGCGGGCGGCAATGTCACCCTGGCCATGGAAAACAGCCCCGACCTG
CTCAGCCAGTCCCAGGTcGAGAGCCTGGTCGGCCGCCGCTACGACATCCTCGACGCCGCCGGCGGCATCGAC
GGGCGCTTCGACGCGGTATTGCCGAACTACCTGTTCCTCGGCGGCACCCTGGACTACGCGGCCAACGCCATC
CGCCTGGATATCGGACGCAACGGCACGGCCCTCGCCAGCGTCGCGCAGACGCCCAACCAGGCGGCGGTCGCT
GGTGCCGTGGAAGCGCTCGGCGCCGGCAACCCGGTCTACGAAAGCCTGCTCCTGTCGGAAAACGCCGCAACC
GCCCAACGGGCCTTCCAACAATTGTCCGGGGAAATCTACCCGGCGCTCGCCGGCCTGTTGCTCAACGACAGC
CGCTATCTGCGTGACAGCGTCGGCGAACGCCTGCGCCAGGCCAGCGACGGCGAGGCCGGCAGGGAGGCTCCC
GAAGGCTGGTTCAAGGCGCTCGGTTCcTGGGGCAAGAGCGCCGATGGCAGCCACGGCAGCGAAGGCTACCGG
CATTCGGTCGGCGGCTTCCTGCTCGGCGTCGACAGCCAGGTCGCCAGCGACACGCGCCTCGGCCTGGTGGCC
GGCTACAGCAACAGCTCGCTGAACATGGACAGCAGCCTGCAATCCTCCGCCAGCATCGACAGCTACCACCTC
GGCGCCTACCTCGGCCGGCAATTGCAGCAATGGCGCCTGAGCCTCGGCGCGGCGCACGCCTGGCACCGCGCC
GAGGTCAAGCGCGACCTGCAATACGGCGCCGTGGCCGGCAAGCAGAAGGCCAAGCTCGACGCACAGAGCAGC
CAGTTGTTCGCCGAGGCCGCCTACGCGCTGGGCTGGCGCAGCCTGGAGCTGGAACCCTTCGCCGGGCTGGCC
TACGTGCACGTCGCCAGCGATGACTTCCGCGAACGCGGTAGTGCCGCGGCCCTGGAGGGTGGCGACGACAAC
CTGGACGCCGCCTTCACCACCCTGGGCCTGCGCGCGAAACGGCATTTCGAGCTGGATGCCGAACGCCGCCTG
GCGCTCTCCGGCACCCTCGGCTGGCGCCACAACCTGAGCGACACCACCCCGCAACGCCACCTGGCGTTCGCC
AGCGGCAGCCAGCCATTCAACGTGGAAAGCGTGGCCCTGTCCCGCGACGCCGCGCTGCTCGGCGTCGACGCC
AGCCTCGCGGTGAATCGCGAAGTGAGCGTGCGGCTGGGCTACAACGGCCTGCTGGGCAGCCGCGAGAAGGAC
CATGGCGTCGGACTGGCCGTCGACTGGCGTTTCTGA
Translation:
MTDDHSFRPRPTSLSAALLLGAWIAQPATAAYVEAGRPGDPASWRSAEYQQDWGLERMRADQAYAAGIDGQG
VKIGEMDSGFDPSHPDTPASRYQPVTASGTYVDGTPFSVSGAMNGNNDSHGTHVGGTLGASRDGVGMHGVAY
AAQVYVANTNQNDSFLFGPTPDPNYFKAAYQALADAGVRAINNSWGSQPKDVSYETLDGLHAAYAQHYGRST
WLDAAAGVSRQGVINVFSAGNSGYANASVRSALPYFQPDLEGHWLAVSGLDQQNGQRYNRCGIAKYWCITTP
GRLINSTMPGGGYANKSGTSMAAPHATGALALVMQRYPYLNNEQALQVLLTTATQLDGTPTGAPTDTVGWGV
PDLGRAMHGPGQLLGRFEANLPAGLRDEWSNPISDSALLQRQAEDAAEHAAWQRTLKDKGWENGLPAGASQQ
ERTDYAIGMARDQAAAQRQYQGSLVKAGAGSLVLSGDSTYRGPTLVDGGLLSVDGSLLSAVEVNAGGTLGGS
GRIGGLLARSGGTVAAGNSIGTLEVAGDLRFESGSTYAVELSESASDRIVASGKASIAGGNVTLAMENSPDL
LSQSQVESLVGRRYDILDAAGGIDGRFDAVLPNYLFLGGTLDYAANAIRLDIGRNGTALASVAQTPNQAAVA
GAVEALGAGNPVYESLLLSENAATAQRAFQQLSGEIYPALAGLLLNDSRYLRDSVGERLRQASDGEAGREAP
EGWFKALGSWGKSADGSHGSEGYRHSVGGFLLGVDSQVASDTRLGLVAGYSNSSLNMDSSLQSSASIDSYHL
GAYLGRQLQQWRLSLGAAHAWHRAEVKRDLQYGAVAGKQKAKLDAQSSQLFAEAAYALGWRSLELEPFAGLA
YVHVASDDFRERGSAAALEGGDDNLDAAFTTLGLRAKRHFELDAERRLALSGTLGWRHNLSDTTPQRHLAFA
SGSQPFNVESVALSRDAALLGVDASLAVNREVSVRLGYNGLLGSREKDHGVGLAVDWRF*
AnnotationID:79GeneID:69
AnnotatorUID: diggins
Modification Date/Time: 2005-07-17 11:11:14
Gene Name:
Confidence Code:3
GeneProduct:putative serine protease
Cell Localization:()
Synonyms:eprS
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:prtB,pspA
Functional Category Confidence Code:5
COGs:pfam00082
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA07702.1| (AJ007828) serine protease [Pseudomonas tolaasii]
          Length = 985

 Score = 1317 bits (3409), Expect = 0.0
 Identities = 657/996 (65%), Positives = 780/996 (78%), Gaps = 13/996 (1%)

>gb|AAF80995.1| (AF216702) PrtB [Pseudomonas fluorescens]
          Length = 1036

 Score =  820 bits (2117), Expect = 0.0
 Identities = 486/1040 (46%), Positives = 634/1040 (60%), Gaps = 93/1040 (8%)







 
Structural Features:
pfam00082, Peptidase_S8, Subtilase family. 
Subtilases are a family of serine proteases.
CD-Length = 301 residues,  97.0% aligned
Score =  132 bits (332), Expect = 2e-31
Identities = 84/310 (27%), Positives = 113/310 (36%), Gaps = 33/310 (10%)






   
Genomic Context:






   
Comment:
other possible gene names: prtB, pspA


















    ReferenceID:141




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17283




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32028




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:69_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:69
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
140
                           PA3535_tr
                           translation of PA3535
                           995
                           99.39
                           99.49
                           0
                           5107.0
     

     
     
13369
                           gb|AAG06923.1|AE004774_1
                           (AE004774) probable serine protease [Pseudomonas aeruginosa PAO1]
                           995
                           99.39
                           99.49
                           0
                           5107.0
     

     
     
13370
                           dbj|BAA36466.1|
                           (AB015053) serine protease homologue [Pseudomonas fluorescens]
                           997
                           66.59
                           79.03
                           14
                           3445.0
     

     
     
13371
                           emb|CAA07702.1|
                           (AJ007828) serine protease [Pseudomonas tolaasii]
                           997
                           65.89
                           78.23
                           14
                           3423.0
     

     
     
13372
                           gb|AAO55169.1|
                           (AE016861) autotransporter, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           1010
                           63.36
                           77.02
                           24
                           3314.0
     

     






      

          
GC ORFID: 43071How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3535Glimmer Score: 

          
Start: 3350706Stop: 3353693

          
Length: 2988


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3535 translation of PA3535Homolog Bit Score 1971.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6407 (ORF: BLASTX 1305.0), psyr_15may02_Scaffold5_revised_gene6408 (ORF: BLASTX 786.0), t_PA3535 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGACGACCACTCCTTCCGCCCTCGCCCCACCTCGTTGTCAGCCGCCCTGCTGCTGGGCGCCTGGATC 
GCCCAGCCGGCCACGGCCGCCTATGTCGAAGCCGGTCGGCCCGGCGATCCGGCCAGTTGGCGCTCCGCCGAA 
TACCAGCAGGACTGGGGCCTGGAACGGATGCGGGCCGACCAGGCCTATGCCGCCGGCATCGACGGCCAGGGC 
GTGAAGATCGGCGAGATGGACTCCGGTTTCGACCCGAGCCATCCGGATACCCCCGCCTCGCGCTACCAGCCG 
GTGACGGCCAGCGGCACCTATGTCGACGGCACGCCGTTCAGCGTCAGCGGCGCGATGAACGGCAACAACGAC 
TCCCACGGCACCCACGTCGGCGGCACCCTCGGTGCCTCGCGCGACGGCGTCGGCATGCACGGGGTGGCCTAC 
GCGGCACAGGTGTACGTCGCCAACACCAACCAGAACGACAGCTTCCTGTTCGGCCCGACGCCCGACCCGAAC 
TATTTCAAGGCCGCCTACCAGGCGCTGGCCGACGCCGGGGTGCGGGCGATCAACAACAGTTGGGGCAGCCAG 
CCCAAGGACGTCAGCTACGAGACCCTCGACGGCCTGCACGCCGCCTATGCCCAGCACTACGGGCGCTCCACC 
TGGCTGGACGCCGCCGCCGGCGTCTCCCGCCAGGGCGTGATCAACGTCTTCAGCGCCGGCAACAGCGGCTAC 
GCCAACGCCAGCGTGCGCTCCGCCCTGCCCTACTTCCAGCCGGACCTGGAAGGCCACTGGCTGGCCGTGTCC 
GGCCTCGACCAGCAGAACGGCCAGCGCTACAACCGCTGCGGCATCGCCAAGTACTGGTGCATCACCACGCCC 
GGCCGCCTGATCAACAGCACCATGCCCGGCGGCGGCTACGCCAACAAGTCCGGTACCTCGATGGCCGCGCCC 
CACGCCACCGGCGCGCTGGCCCTGGTCATGCAGCGCTATCCGTACCTGAACAACGAGCAGGCGCTGCAGGTT 
CTGCTGACCACCGCCACCCAGCTCGACGGCACGCCGACCGGCGCCCCCACCGACACCGTCGGCTGGGGCGTG 
CCGGATCTCGGTCGGGCGATGCATGGGCCTGGACAATTGCTCGGCCGCTTCGAGGCCAACCTCCCGGCCGGC 
CTGCGCGACGAATGGAGCAACCCGATTTCCGATAGCGCCCTGCTCCAGCGCCAGGCCGAGGACGCCGCCGAG 
CACGCGGCCTGGCAGCGGACGCTGAAGGACAAGGGCTGGGAAAACGGCTTGCCAGCCGGCGCCAGCCAGCAG 
GAACGCACCGACTATGCCATCGGCATGGCCCGCGACCAGGCCGCCGCCCAGCGCCAGTACCAGGGCAGCCTG 
GTCAAGGCCGGTGCCGGCAGCCTGGTCCTGAGCGGCGACAGCACCTATCGCGGGCCGACCCTGGTCGATGGC 
GGGCTGCTCAGCGTCGACGGTTCGCTGCTGTCCGCCGTCGAAGTCAATGCCGGCGGCACCCTCGGCGGCAGC 
GGCAGGATCGGCGGCCTGCTGGCGCGCTCCGGCGGCACGGTGGCCGCGGGCAACTCCATCGGCACCCTGGAG 
GTCGCCGGGGACCTGCGCTTCGAATCCGGCTCGACCTACGCGGTGGAGCTTTCGGAAAGCGCCAGCGACCGG 
ATCGTCGCCAGCGGCAAGGCGAGCATCGCGGGCGGCAATGTCACCCTGGCCATGGAAAACAGCCCCGACCTG 
CTCAGCCAGTCCCAGGTcGAGAGCCTGGTCGGCCGCCGCTACGACATCCTCGACGCCGCCGGCGGCATCGAC 
GGGCGCTTCGACGCGGTATTGCCGAACTACCTGTTCCTCGGCGGCACCCTGGACTACGCGGCCAACGCCATC 
CGCCTGGATATCGGACGCAACGGCACGGCCCTCGCCAGCGTCGCGCAGACGCCCAACCAGGCGGCGGTCGCT 
GGTGCCGTGGAAGCGCTCGGCGCCGGCAACCCGGTCTACGAAAGCCTGCTCCTGTCGGAAAACGCCGCAACC 
GCCCAACGGGCCTTCCAACAATTGTCCGGGGAAATCTACCCGGCGCTCGCCGGCCTGTTGCTCAACGACAGC 
CGCTATCTGCGTGACAGCGTCGGCGAACGCCTGCGCCAGGCCAGCGACGGCGAGGCCGGCAGGGAGGCTCCC 
GAAGGCTGGTTCAAGGCGCTCGGTTCcTGGGGCAAGAGCGCCGATGGCAGCCACGGCAGCGAAGGCTACCGG 
CATTCGGTCGGCGGCTTCCTGCTCGGCGTCGACAGCCAGGTCGCCAGCGACACGCGCCTCGGCCTGGTGGCC 
GGCTACAGCAACAGCTCGCTGAACATGGACAGCAGCCTGCAATCCTCCGCCAGCATCGACAGCTACCACCTC 
GGCGCCTACCTCGGCCGGCAATTGCAGCAATGGCGCCTGAGCCTCGGCGCGGCGCACGCCTGGCACCGCGCC 
GAGGTCAAGCGCGACCTGCAATACGGCGCCGTGGCCGGCAAGCAGAAGGCCAAGCTCGACGCACAGAGCAGC 
CAGTTGTTCGCCGAGGCCGCCTACGCGCTGGGCTGGCGCAGCCTGGAGCTGGAACCCTTCGCCGGGCTGGCC 
TACGTGCACGTCGCCAGCGATGACTTCCGCGAACGCGGTAGTGCCGCGGCCCTGGAGGGTGGCGACGACAAC 
CTGGACGCCGCCTTCACCACCCTGGGCCTGCGCGCGAAACGGCATTTCGAGCTGGATGCCGAACGCCGCCTG 
GCGCTCTCCGGCACCCTCGGCTGGCGCCACAACCTGAGCGACACCACCCCGCAACGCCACCTGGCGTTCGCC 
AGCGGCAGCCAGCCATTCAACGTGGAAAGCGTGGCCCTGTCCCGCGACGCCGCGCTGCTCGGCGTCGACGCC 
AGCCTCGCGGTGAATCGCGAAGTGAGCGTGCGGCTGGGCTACAACGGCCTGCTGGGCAGCCGCGAGAAGGAC 
CATGGCGTCGGACTGGCCGTCGACTGGCGTTTCTGA