ViewGene
Locus Name:
GeneID:
Location:

GeneID:689
TrivialName: PA14_68580
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_68580
PAO1 Ortholog Locus:PA5192
Sequence Length:1542
Protein Length:513
Start: 6118219
Stop: 6116678
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08577.1|AE004932_4 (AE004932) phosphoenolpyruvate carboxykinase [Pseudomonas aeruginosa PAO1]
Identities = 511/513 (99%), Positives = 512/513 (99%)
Sequence:
ATGACGCAAGCCAACAACGCCGTGTACACCGATATCAGCGCCGCCCAACTGGTAGAAGAAGCCATTCGCCGC
GGTGAGGGCGAACTGGCCGCCAACGGCTCGCTGGTCGTACGCACCGGCCATCGCACCGGCCGTTCGCCGGTC
GACCGTTTCATCGTCGAAGAGCCGAGCACCAAGGACGCGATCGCCTGGGGCAACATCAACCGTCCGTTCCCG
GCCGACAAGTTCGATGCGCTGTGGGCCCGCGTGGAAGCGTTCAACAACGCCCAGGACCATTTCGTTTCCCAT
GTTCATGTAGGTTCCGCCGAGGCTTACTACCTGCCGGTGAAGATGACCACCGCTACCGCCTGGCAGAATCTG
TTCGGCCGTTGCCTGTTCATCGAGCCGGAGCAGTACAACCCGGCCGGCAAGGACGAGTGGCAGGTCCTCAAT
GTCGCCAACTTCGAATGCGTGCCCGAGCGCGACGGCACCAACTCCGACGGTTGCGTGATCCTCAATTTCGCC
CAGAAGAAAGTGCTGATCGCAGGCATGCGCTATGCCGGCGAGATGAAGAAAGCCATGTTCTCCGTGCAGAAC
TTCCTGCTGCCCGAGCGCGACGTGCTGCCGATGCACTGCGCTGCCAACATCGGCGAAGCGGGCGATGTGACC
CTGTTCTTCGGCCTGTCCGGCACCGGCAAGACCACCCTGTCCGCTGATGAAAGCCGTTACCTGATCGGTGAC
GACGAGCACGGCTGGGGCGAGGGCGTGGTGTTCAACGTCGAAGGCGGTTGCTATGCCAAGTGCATCGACCTG
TCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGTTCGGCGCCGTGCTGGAGAACGTGGTTCTCGAC
GAAGAGCGCGTGCCCAACTACGCCGATGACAGCCTGACCCAGAACAGCCGCGCCGCCTACCCGCTGGAGCAC
GTCGAGAAGCGTTCGGAGAAGAACCTCGGCGGCGAGCCCAACGCGGTGATCTTCCTGACCTGCGACCTGACC
GGCGTACTGCCGCCGGTATCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCG
CTGGTCGGCTCCACCGAGATGGGTTCCGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCTCCC
TTCTTCCCGCGTCCGGCCGGTGTCTACGCCGAGCTGCTGATCAAGCGTATCAAGGCCTTCGGCTCCAAGGTC
TACCTGGTCAACACCGGCTGGACCGGCGGTGGCTACGGCGTCGGCAAGCGCTTCAACATCCCGACCACCCGT
GGCGTGATCGCGGCGATCCAGAGCGGCGCGCTGATCGGCGCGGAAACCGAGCACCTGGACATCATCAACCTG
GACGTGCCGAAGGTCGTTCCGGGCGTCGAGACCAATCTGCTCAACCCGCGCAACACCTGGGCCGACAAGGCT
GCCTACGACGAGGCGGCGAAGGGCCTGGCCAAGCAGTTCATCGAAAACTTCAAGAAATTCGAGGTTTCCGAT
GCGATCAAGGCCGCCGGCCCGCAGCTGTAA
Translation:
MTQANNAVYTDISAAQLVEEAIRRGEGELAANGSLVVRTGHRTGRSPVDRFIVEEPSTKDAIAWGNINRPFP
ADKFDALWARVEAFNNAQDHFVSHVHVGSAEAYYLPVKMTTATAWQNLFGRCLFIEPEQYNPAGKDEWQVLN
VANFECVPERDGTNSDGCVILNFAQKKVLIAGMRYAGEMKKAMFSVQNFLLPERDVLPMHCAANIGEAGDVT
LFFGLSGTGKTTLSADESRYLIGDDEHGWGEGVVFNVEGGCYAKCIDLSEKNEPVIWKAIKFGAVLENVVLD
EERVPNYADDSLTQNSRAAYPLEHVEKRSEKNLGGEPNAVIFLTCDLTGVLPPVSILNNEQAAYHFLSGYTA
LVGSTEMGSGGGIKSTFSTCFGAPFFPRPAGVYAELLIKRIKAFGSKVYLVNTGWTGGGYGVGKRFNIPTTR
GVIAAIQSGALIGAETEHLDIINLDVPKVVPGVETNLLNPRNTWADKAAYDEAAKGLAKQFIENFKKFEVSD
AIKAAGPQL*
AnnotationID:698GeneID:689
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 13:01:36
Gene Name:pckA
Confidence Code:2
GeneProduct:phosphoenolpyruvate carboxykinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1866,pfam01293
Secondary Functional Category(ies):carbon compound catabolism
EC Number:4.1.1.49
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53785.1| (AE016856) phosphoenolpyruvate carboxykinase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 514

 Score =  869 bits (2246), Expect = 0.0
 Identities = 429/514 (83%), Positives = 460/514 (89%), Gaps = 1/514 (0%)

>gb|AAN53249.1|AE015466_9 (AE015466) phosphoenolpyruvate carboxykinase (ATP) [Shewanella
           oneidensis MR-1]
          Length = 513

 Score =  711 bits (1834), Expect = 0.0
 Identities = 344/512 (67%), Positives = 411/512 (80%)

>gb|AAO91576.1| (AE016966) phosphoenolpyruvate carboxykinase (ATP) [Coxiella
           burnetii RSA 493]
          Length = 517

 Score =  624 bits (1608), Expect = e-178
 Identities = 305/509 (59%), Positives = 378/509 (74%), Gaps = 1/509 (0%)








 
Structural Features:
COG1866, PckA, Phosphoenolpyruvate carboxykinase (ATP) 
[Energy production and conversion]
CD-Length = 529 residues,  97.4% aligned
Score =  751 bits (1940), Expect = 0.0
Identities = 286/516 (55%), Positives = 343/516 (66%), Gaps = 4/516 (0%)

pfam01293, PEPCK_ATP, Phosphoenolpyruvate carboxykinase.
CD-Length = 471 residues,  99.2% aligned
Score =  543 bits (1401), Expect = 1e-155
Identities = 230/468 (49%), Positives = 305/468 (65%), Gaps = 7/468 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1381




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18523




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34905




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:689_cdd.html


    		




 
URL:

















    ReferenceID:35767




  
Author/Investigator(s):
        Laivenieks M, Vieille C, Zeikus JG.





  
Title:
        Cloning, sequencing, and overexpression of the Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase (pckA) gene.




  
PubMed:
        9172347


MedLine:
        




    
Source:
Appl Environ Microbiol. 1997 Jun;63(6):2273-80.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9172347

















    ReferenceID:35768




  
Author/Investigator(s):
        Osteras M, Finan TM, Stanley J.





  
Title:
        Site-directed mutagenesis and DNA sequence of pckA of Rhizobium NGR234, encoding phosphoenolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype.




  
PubMed:
        1720862


MedLine:
        




    
Source:
Mol Gen Genet. 1991 Nov;230(1-2):257-69.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1720862

















    ReferenceID:35769




  
Author/Investigator(s):
        Medina V, Pontarollo R, Glaeske D, Tabel H, Goldie H.





  
Title:
        Sequence of the pckA gene of Escherichia coli K-12: relevance to genetic and allosteric regulation and homology of E. coli phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma brucei and Saccharomyces cerevisiae.




  
PubMed:
        1701430


MedLine:
        




    
Source:
J Bacteriol. 1990 Dec;172(12):7151-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1701430









           
Homologs By Global Alignment 

           

       



     
Gene ID:689
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1166
                           PA5192_tr
                           translation of PA5192
                           513
                           99.61
                           99.80
                           0
                           2686.0
     

     
     
42763
                           gb|AAG08577.1|AE004932_4
                           (AE004932) phosphoenolpyruvate carboxykinase [Pseudomonas aeruginosa PAO1]
                           513
                           99.61
                           99.80
                           0
                           2686.0
     

     
     
42764
                           gb|AAO53785.1|
                           (AE016856) phosphoenolpyruvate carboxykinase [Pseudomonas syringae pv. tomato str. DC3000]
                           514
                           83.46
                           89.49
                           1
                           2248.0
     

     
     
42765
                           gb|AAN53249.1|AE015466_9
                           (AE015466) phosphoenolpyruvate carboxykinase (ATP) [Shewanella oneidensis MR-1]
                           513
                           67.05
                           80.11
                           0
                           1833.0
     

     
     
42766
                           gb|AAO91576.1|
                           (AE016966) phosphoenolpyruvate carboxykinase (ATP) [Coxiella burnetii RSA 493]
                           517
                           59.38
                           73.50
                           4
                           1605.0
     

     






      

          
GC ORFID: 43691How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5192Glimmer Score: 

          
Start: 5381905Stop: 5380364

          
Length: 1542


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5192 translation of PA5192Homolog Bit Score 1039.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3973 (ORF: BLASTX 872.0), t_PA5192 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGCAAGCCAACAACGCCGTGTACACCGATATCAGCGCCGCCCAACTGGTAGAAGAAGCCATTCGCCGC 
GGTGAGGGCGAACTGGCCGCCAACGGCTCGCTGGTCGTACGCACCGGCCATCGCACCGGCCGTTCGCCGGTC 
GACCGTTTCATCGTCGAAGAGCCGAGCACCAAGGACGCGATCGCCTGGGGCAACATCAACCGTCCGTTCCCG 
GCCGACAAGTTCGATGCGCTGTGGGCCCGCGTGGAAGCGTTCAACAACGCCCAGGACCATTTCGTTTCCCAT 
GTTCATGTAGGTTCCGCCGAGGCTTACTACCTGCCGGTGAAGATGACCACCGCTACCGCCTGGCAGAATCTG 
TTCGGCCGTTGCCTGTTCATCGAGCCGGAGCAGTACAACCCGGCCGGCAAGGACGAGTGGCAGGTCCTCAAT 
GTCGCCAACTTCGAATGCGTGCCCGAGCGCGACGGCACCAACTCCGACGGTTGCGTGATCCTCAATTTCGCC 
CAGAAGAAAGTGCTGATCGCAGGCATGCGCTATGCCGGCGAGATGAAGAAAGCCATGTTCTCCGTGCAGAAC 
TTCCTGCTGCCCGAGCGCGACGTGCTGCCGATGCACTGCGCTGCCAACATCGGCGAAGCGGGCGATGTGACC 
CTGTTCTTCGGCCTGTCCGGCACCGGCAAGACCACCCTGTCCGCTGATGAAAGCCGTTACCTGATCGGTGAC 
GACGAGCACGGCTGGGGCGAGGGCGTGGTGTTCAACGTCGAAGGCGGTTGCTATGCCAAGTGCATCGACCTG 
TCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGTTCGGCGCCGTGCTGGAGAACGTGGTTCTCGAC 
GAAGAGCGCGTGCCCAACTACGCCGATGACAGCCTGACCCAGAACAGCCGCGCCGCCTACCCGCTGGAGCAC 
GTCGAGAAGCGTTCGGAGAAGAACCTCGGCGGCGAGCCCAACGCGGTGATCTTCCTGACCTGCGACCTGACC 
GGCGTACTGCCGCCGGTATCGATCCTGAACAACGAGCAGGCGGCCTACCACTTCCTGTCCGGCTACACCGCG 
CTGGTCGGCTCCACCGAGATGGGTTCCGGCGGCGGCATCAAGTCGACCTTCTCCACCTGCTTCGGCGCTCCC 
TTCTTCCCGCGTCCGGCCGGTGTCTACGCCGAGCTGCTGATCAAGCGTATCAAGGCCTTCGGCTCCAAGGTC 
TACCTGGTCAACACCGGCTGGACCGGCGGTGGCTACGGCGTCGGCAAGCGCTTCAACATCCCGACCACCCGT 
GGCGTGATCGCGGCGATCCAGAGCGGCGCGCTGATCGGCGCGGAAACCGAGCACCTGGACATCATCAACCTG 
GACGTGCCGAAGGTCGTTCCGGGCGTCGAGACCAATCTGCTCAACCCGCGCAACACCTGGGCCGACAAGGCT 
GCCTACGACGAGGCGGCGAAGGGCCTGGCCAAGCAGTTCATCGAAAACTTCAAGAAATTCGAGGTTTCCGAT 
GCGATCAAGGCCGCCGGCCCGCAGCTGTAA