ViewGene
Locus Name:
GeneID:
Location:

GeneID:6868
TrivialName: PA14_68010
AnnotatorUID: danlee
Modification Date/Time:2006-08-22 20:08:33
LocusName:PA14_68010
PAO1 Ortholog Locus:
Sequence Length:549
Protein Length:182
Start: 6069532
Stop: 6070080
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08534.1|AE004927_12 (AE004927) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 176/177 (99%), Positives = 176/177 (99%). Putative frame-shift splits PAO1 PA5149 into two ORFs: GID6868 (PA14_68010) and GID6869 (PA14_68020).
Sequence:
ATGACCCCATCCCTCTCTGGCCTGCCTCCGATCCGCACCGCGCTGATCGGCTACGGCTTCGCCGGCAAGACC
TTCCACGCCCCGCTGATCCGCGCGGTACCGGCGTTGTGCCTGGAGCACGTGGTCTCCCGCGACCCTGTGCGG
GTGCACGCCGACCTGCCCGAAGCCCGGGTCAGCGCCGATCCGGATGAGGCCATCGAGGCCGCCGACATCGAC
CTGGTGGTCATCGCCTCGCCCAATGCCACCCACGCCAGCCTCGCCGAACGGGCCTTGCGCGCCGGCAAGCAC
GTGGTGGTGGACAAGCCGTTCACCGTCACCCTGGCCGAAGCCCGGCGCCTCGCCGACCTCGCCCGCGAACGG
CCTGGCCAGGTCCTCTCGGTGTTCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTG
CAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGcGAC
Cgctggcgcgaacacgacctgcccggcttccgggctgtggtttga
Translation:
MTPSLSGLPPIRTALIGYGFAGKTFHAPLIRAVPALCLEHVVSRDPVRVHADLPEARVSADPDEAIEAADID
LVVIASPNATHASLAERALRAGKHVVVDKPFTVTLAEARRLADLARERPGQVLSVFQNRRFDSDFLAVEAAL
QAGSIGPVRYFESAIERFRPQVRDRWREHDLPGFRAVV*
AnnotationID:6869GeneID:6868
AnnotatorUID: danlee
Modification Date/Time: 2006-03-21 11:11:31
Gene Name:
Confidence Code:4
GeneProduct:putative dehydrogenase, N-terminus
Cell Localization:()
Synonyms:PA5149 N-terminus
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(14) Hypothetical, unclassified, unknown
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|9951449|gb|AAG08534.1|  conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
 gi|15600342|ref|NP_253836.1|  hypothetical protein PA5149 [Pseudomonas aeruginosa PAO1]
 gi|84317781|ref|ZP_00966218.1|  COG0673: Predicted dehydrogenases and related proteins [Pseudomonas 
aeruginosa C3719]
Length=360

 Score =  330 bits (845),  Expect = 2e-89, Method: Composition-based stats.
 Identities = 176/177 (99%), Positives = 176/177 (99%), Gaps = 0/177 (0%)

gi|84323764|ref|ZP_00971831.1|  COG0673: Predicted dehydrogenases and related proteins [Pseudomonas 
aeruginosa 2192]
Length=360

 Score =  330 bits (845),  Expect = 2e-89, Method: Composition-based stats.
 Identities = 176/177 (99%), Positives = 176/177 (99%), Gaps = 0/177 (0%)








 
Structural Features:
COG0673, MviM, Predicted dehydrogenases and related proteins [General function prediction only].
             CD-Length = 342 residues, only  46.8% aligned
             Score = 91.8 bits (227), Expect = 5e-20
             Aligns with N-terminus of COG0673

pfam01408, GFO_IDH_MocA, Oxidoreductase family, NAD-binding Rossmann fold. This family of 
enzymes utilise NADP or NAD. This family is called the GFO/IDH/MOCA family..
             CD-Length = 120 residues,  81.7% aligned
             Score = 68.7 bits (168), Expect = 4e-13






   
Genomic Context:






   
Comment:
Putative frame-shift splits PAO1 PA5149 into two ORFs: GID6868 (PA14_68010) and GID6869 (PA14_68020).


















    ReferenceID:13444




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30819




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34917




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6868_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6868
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
8046
                           PA5149_tr
                           translation of PA5149
                           360
                           48.88
                           48.88
                           178
                           882.0
     

     
     
202706
                           gb|AAG08534.1|AE004927_12
                           (AE004927) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           360
                           48.88
                           48.88
                           178
                           882.0
     

     
     
202707
                           emb|CAD18212.1|
                           (AL646082) PROBABLE OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum]
                           387
                           28.42
                           33.85
                           209
                           472.0
     

     
     
202727
                           emb|CAB65577.1|
                           (AL939114) putative oxidoreductase. [Streptomyces coelicolor A3(2)]
                           361
                           27.14
                           33.79
                           180
                           425.0
     

     
     
202725
                           emb|CAC91063.1|
                           (AJ414151) putative oxidoreductase [Yersinia pestis CO92]
                           356
                           26.96
                           33.42
                           181
                           420.0
     

     






      

          
GC ORFID: 49870How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 5333218Stop: 5333766

          
Length: 549


          
Start Codon: ATGTruncated Start:

          
Stop Codon: tgaTruncated Stop: 

          
Homolog: t_PA5149 translation of PA5149Homolog Bit Score 347.0

          
Other Homologs:  t_PA5149 translation of PA5149 (BLASTP 347), psyr_15may02_Scaffold2_revised_gene3044 related to: gi|6689180|emb|CAB65577.1| (AL136058) putative oxidoreductase. [Streptomyces coelicolor] (BLASTP 114)

          
GC ORF Sequence

           
ATGACCCCATCCCTCTCTGGCCTGCCTCCGATCCGCACCGCGCTGATCGGCTACGGCTTCGCCGGCAAGACC 
TTCCACGCCCCGCTGATCCGCGCGGTACCGGCGTTGTGCCTGGAGCACGTGGTCTCCCGCGACCCTGTGCGG 
GTGCACGCCGACCTGCCCGAAGCCCGGGTCAGCGCCGATCCGGATGAGGCCATCGAGGCCGCCGACATCGAC 
CTGGTGGTCATCGCCTCGCCCAATGCCACCCACGCCAGCCTCGCCGAACGGGCCTTGCGCGCCGGCAAGCAC 
GTGGTGGTGGACAAGCCGTTCACCGTCACCCTGGCCGAAGCCCGGCGCCTCGCCGACCTCGCCCGCGAACGG 
CCTGGCCAGGTCCTCTCGGTGTTCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTG 
CAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGcGAC 
Cgctggcgcgaacacgacctgcccggcttccgggctgtggtttga