ViewGene
Locus Name:
GeneID:
Location:

GeneID:685
TrivialName: PA14_19470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19470
PAO1 Ortholog Locus:PA3452
Sequence Length:1572
Protein Length:523
Start: 1684298
Stop: 1682727
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06840.1|AE004766_1 (AE004766) malate:quinone oxidoreductase [Pseudomonas aeruginosa PAO1]
Identities = 522/523 (99%), Positives = 522/523 (99%)
Sequence:
ATGAAAAAAATCTTATTGATGCTTTTGTGCGTCAGCGTGCTGGGTTGCTCCAAAAACAGCGTCGAATCGGAA
AAACCGGTCGACGTGCTCCTGATCGGTGGCGGCATCATGAGCGCCACCCTGGGTACCTATCTGAACGAACTG
GAACCGGGCTGGACCATCGAGATGGTCGAGCGCCTGGACAAGGTCGCCGAGGAAAGCTCCAACGGCTGGAAC
AACGCGGGCACCGGTCACTCGGCGTTCTGCGAACTGAACTACACCAGCGAGGCCGCGGACGGCTCGATGGAT
ATCAGCAAGGCGGTGGCGATCAACGAGAACTTCGAGATCTCCAAGCAGTTCTGGGCCTACCAGGTCGACCGC
AAGGTGCTGAACGATCCGAAGTCGTTCATCAACAACGTCCCGCACATGAGCTTCGTCTGGGGCGACGACAAC
GTCGCCTTCCTCAAGAAGCGCCACGCCGCCCTGCAGCACAGCTCGCTGTTCCGCGGCATGGAGTACTCGGAA
GACCCCGAGCAGATCAAGCAGTGGGTGCCGCTGGTGATGGAAGGCCGCGAGCCGGGGCAGAAGATCGCCGCC
ACGCGCATGTCGATCGGCACCGACGTCAACTTCGGCGAGATCACCCGCCAGCTGGTGGGCTCGCTGTCGGCC
AAGGACACCTTCAAGCTGCGCCTGCAACATGAGGTCCGCGACCTCAAGCGCAACGACGACAACACCTGGACC
GTGACCATGGCCGACCTGGCCAACGGCGACAAGGAAACCAGCGTCAAGGCCAGGTTCGTCTTCATCGGCGCC
GGTGGCGGCGCGCTGAAGCTGCTGCAGATGTCCGGTATCCCCGAGGCCGAAGGCTATGCCGGCTTCCCGGTG
GGCGGTTCGTTCCTCGCCACCACCAACCCGGACGTGGTCAAGCGCCATCTGGCCAAGGTCTACGGCAAGGCT
TCGGTGGGCTCGCCGCCGATGTCGGTGCCGCACCTCGACACCCGCATGATCGACGGCAAGCCGGTTCTGCTG
TTCGGTCCGTTCGCCACCTTCTCCACCAAGTTCCTGAAGAACGGCTCGCTGTGGGACCTGCCGGGCTCGGTG
ACCAGCAGCAACATCGGCCCGATGTTCAACGCCGGCATCGACAACTTCGATCTCAGCCAGTACCTGATCGGC
CAGCTGATGCTCAGCCAGGACGACCGCATGGCCTCGCTGCGCGAGTACTTCCCGGAAGCCCGCGACGAGGAC
TGGAAGCTGGTGCAGGCCGGCCAGCGCGTGCAGATCATCAAGAAGGACGCCGAGAAAGGCGGCGTACTGCAA
TTCGGCACCGAAGTGGTGACCGCCGCCGACGGCTCCGTCGCCGCCCTGCTCGGCGCCTCGCCGGGCGCCTCG
ACCGCCGCACCGATCATGCTGTCGGTGCTGGAGAAGGCCTTCAAGGACAAGGTCGCCACCCCCgAGTGGCAG
GCACGCCTGAAGGAAATCGTGCCGTCTTACGGGCGCAAGCTGAACAACGACATCGAGCTGACCAACAGCACC
CGTGCCTGGAGCAGCGAGCGCCTGCAACTGATCCACGTGCCGGTACAGCCGGAGGCCTGA
Translation:
MKKILLMLLCVSVLGCSKNSVESEKPVDVLLIGGGIMSATLGTYLNELEPGWTIEMVERLDKVAEESSNGWN
NAGTGHSAFCELNYTSEAADGSMDISKAVAINENFEISKQFWAYQVDRKVLNDPKSFINNVPHMSFVWGDDN
VAFLKKRHAALQHSSLFRGMEYSEDPEQIKQWVPLVMEGREPGQKIAATRMSIGTDVNFGEITRQLVGSLSA
KDTFKLRLQHEVRDLKRNDDNTWTVTMADLANGDKETSVKARFVFIGAGGGALKLLQMSGIPEAEGYAGFPV
GGSFLATTNPDVVKRHLAKVYGKASVGSPPMSVPHLDTRMIDGKPVLLFGPFATFSTKFLKNGSLWDLPGSV
TSSNIGPMFNAGIDNFDLSQYLIGQLMLSQDDRMASLREYFPEARDEDWKLVQAGQRVQIIKKDAEKGGVLQ
FGTEVVTAADGSVAALLGASPGASTAAPIMLSVLEKAFKDKVATPEWQARLKEIVPSYGRKLNNDIELTNST
RAWSSERLQLIHVPVQPEA*
AnnotationID:694GeneID:685
AnnotatorUID: danlee
Modification Date/Time: 2006-01-30 13:01:12
Gene Name:mqoA
Confidence Code:2
GeneProduct:malate:quinone oxidoreductase
Cell Localization:()
Synonyms:yojH
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam06039,COG0579
Secondary Functional Category(ies):Energy metabolism
EC Number:1.1.99.16
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN81205.1|AE016763_164 (AE016763) Malate:quinone oxidoreductase [Escherichia coli CFT073]
          Length = 548

 Score =  717 bits (1852), Expect = 0.0
 Identities = 355/519 (68%), Positives = 423/519 (81%), Gaps = 6/519 (1%)

>gb|AAL41826.1| (AE009048) malate:quinone oxidoreductase [Agrobacterium tumefaciens
           str. C58 (U. Washington)]
          Length = 595

 Score =  665 bits (1717), Expect = 0.0
 Identities = 332/498 (66%), Positives = 396/498 (79%), Gaps = 4/498 (0%)

>gb|AAO29586.1| (AE012559) malate:quinone oxidoreductase [Xylella fastidiosa
           Temecula1]
          Length = 562

 Score =  571 bits (1472), Expect = e-162
 Identities = 286/497 (57%), Positives = 359/497 (72%), Gaps = 2/497 (0%)






 
Structural Features:
pfam06039, Mqo, Malate:quinone oxidoreductase (Mqo).
CD-Length = 489 residues,  99.8% aligned
Score =  860 bits (2224), Expect = 0.0
Identities = 342/491 (69%), Positives = 397/491 (80%), Gaps = 3/491 (0%)

COG0579, Predicted dehydrogenase 
CD-Length = 429 residues, 100.0% aligned
Score =  413 bits (1063), Expect = 2e-116
Identities = 181/478 (37%), Positives = 249/478 (52%), Gaps = 52/478 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1373




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18515




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32118




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:685_cdd.html


    		




 
URL:

















    ReferenceID:34088




  
Author/Investigator(s):
        Gorisch H.





  
Title:
        The ethanol oxidation system and its regulation in Pseudomonas aeruginosa.




  
PubMed:
        12686116


MedLine:
        




    
Source:
Biochim Biophys Acta. 2003 Apr 11;1647(1-2):98-102.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12686116

















    ReferenceID:34089




  
Author/Investigator(s):
        Kretzschmar U, Ruckert A, Jeoung JH, Gorisch H.





  
Title:
        Malate:quinone oxidoreductase is essential for growth on ethanol or acetate in Pseudomonas aeruginosa.




  
PubMed:
        12480887


MedLine:
        




    
Source:
Microbiology. 2002 Dec;148(Pt 12):3839-47.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12480887

















    ReferenceID:34090




  
Author/Investigator(s):
        Molenaar D, van der Rest ME, Petrovic S.





  
Title:
        Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum.




  
PubMed:
        9660197


MedLine:
        




    
Source:
Eur J Biochem. 1998 Jun 1;254(2):395-403.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9660197









           
Homologs By Global Alignment 

           

       



     
Gene ID:685
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1161
                           PA3452_tr
                           translation of PA3452
                           523
                           99.80
                           99.80
                           0
                           2693.0
     

     
     
42707
                           gb|AAG06840.1|AE004766_1
                           (AE004766) malate:quinone oxidoreductase [Pseudomonas aeruginosa PAO1]
                           523
                           99.80
                           99.80
                           0
                           2693.0
     

     
     
42708
                           gb|AAC75270.1|
                           (AE000310) orf, hypothetical protein [Escherichia coli K12]
                           550
                           65.81
                           78.00
                           29
                           1864.0
     

     
     
42709
                           dbj|BAA15993.1|
                           (D90850) ORF_ID:o372#5~similar to [SwissProt Accession Number P33940] [Escherichia coli]
                           550
                           65.81
                           78.00
                           29
                           1864.0
     

     
     
42712
                           gb|AAG57345.1|AE005453_2
                           (AE005453) orf, hypothetical protein [Escherichia coli O157:H7 EDL933]
                           550
                           65.63
                           78.00
                           29
                           1860.0
     

     






      

          
GC ORFID: 43687How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3452Glimmer Score: 

          
Start: 3269634Stop: 3271205

          
Length: 1572


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3452 translation of PA3452Homolog Bit Score 1041.0

          
Other Homologs:  t_PA4640 (ORF: BLASTX 544.0), psyr_15may02_Scaffold3_revised_gene5069 (ORF: BLASTX 536.0), t_PA3452 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAAAAATCTTATTGATGCTTTTGTGCGTCAGCGTGCTGGGTTGCTCCAAAAACAGCGTCGAATCGGAA 
AAACCGGTCGACGTGCTCCTGATCGGTGGCGGCATCATGAGCGCCACCCTGGGTACCTATCTGAACGAACTG 
GAACCGGGCTGGACCATCGAGATGGTCGAGCGCCTGGACAAGGTCGCCGAGGAAAGCTCCAACGGCTGGAAC 
AACGCGGGCACCGGTCACTCGGCGTTCTGCGAACTGAACTACACCAGCGAGGCCGCGGACGGCTCGATGGAT 
ATCAGCAAGGCGGTGGCGATCAACGAGAACTTCGAGATCTCCAAGCAGTTCTGGGCCTACCAGGTCGACCGC 
AAGGTGCTGAACGATCCGAAGTCGTTCATCAACAACGTCCCGCACATGAGCTTCGTCTGGGGCGACGACAAC 
GTCGCCTTCCTCAAGAAGCGCCACGCCGCCCTGCAGCACAGCTCGCTGTTCCGCGGCATGGAGTACTCGGAA 
GACCCCGAGCAGATCAAGCAGTGGGTGCCGCTGGTGATGGAAGGCCGCGAGCCGGGGCAGAAGATCGCCGCC 
ACGCGCATGTCGATCGGCACCGACGTCAACTTCGGCGAGATCACCCGCCAGCTGGTGGGCTCGCTGTCGGCC 
AAGGACACCTTCAAGCTGCGCCTGCAACATGAGGTCCGCGACCTCAAGCGCAACGACGACAACACCTGGACC 
GTGACCATGGCCGACCTGGCCAACGGCGACAAGGAAACCAGCGTCAAGGCCAGGTTCGTCTTCATCGGCGCC 
GGTGGCGGCGCGCTGAAGCTGCTGCAGATGTCCGGTATCCCCGAGGCCGAAGGCTATGCCGGCTTCCCGGTG 
GGCGGTTCGTTCCTCGCCACCACCAACCCGGACGTGGTCAAGCGCCATCTGGCCAAGGTCTACGGCAAGGCT 
TCGGTGGGCTCGCCGCCGATGTCGGTGCCGCACCTCGACACCCGCATGATCGACGGCAAGCCGGTTCTGCTG 
TTCGGTCCGTTCGCCACCTTCTCCACCAAGTTCCTGAAGAACGGCTCGCTGTGGGACCTGCCGGGCTCGGTG 
ACCAGCAGCAACATCGGCCCGATGTTCAACGCCGGCATCGACAACTTCGATCTCAGCCAGTACCTGATCGGC 
CAGCTGATGCTCAGCCAGGACGACCGCATGGCCTCGCTGCGCGAGTACTTCCCGGAAGCCCGCGACGAGGAC 
TGGAAGCTGGTGCAGGCCGGCCAGCGCGTGCAGATCATCAAGAAGGACGCCGAGAAAGGCGGCGTACTGCAA 
TTCGGCACCGAAGTGGTGACCGCCGCCGACGGCTCCGTCGCCGCCCTGCTCGGCGCCTCGCCGGGCGCCTCG 
ACCGCCGCACCGATCATGCTGTCGGTGCTGGAGAAGGCCTTCAAGGACAAGGTCGCCACCCCCgAGTGGCAG 
GCACGCCTGAAGGAAATCGTGCCGTCTTACGGGCGCAAGCTGAACAACGACATCGAGCTGACCAACAGCACC 
CGTGCCTGGAGCAGCGAGCGCCTGCAACTGATCCACGTGCCGGTACAGCCGGAGGCCTGA