ViewGene
Locus Name:
GeneID:
Location:

GeneID:675
TrivialName: PA14_59250
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_59250
PAO1 Ortholog Locus:
Sequence Length:1638
Protein Length:545
Start: 5278639
Stop: 5280276
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAP84210.1| (AY273869) PilN2 [Pseudomonas aeruginosa]
Identities = 536/537 (99%), Positives = 537/537 (100%)
Sequence:
GTGAACGAGTCGGCGGATCGTGTCGAAGCTACGGCAGATTCCGCGTCTACGATCGCAGCGCAGGTGCGCAAC
ACCCGACCGGATCGGCGCGATACGGTGGTGTTCTCCGACAAACCCTGGGTCAGCACGAAACCCCTAAGCGTT
TCGCACACCTTGTCCAGTGACTGCATCGTGACGTGGCGCCCTGCAGGCGCAGCGTCGCTGCAGGAGGCCGCC
CAGGAAGTCATCAACCAATGCCACATGGCGGTCAGTATCACGCCCGACGCGCTGAACCCGGCCGCCTTCGCC
GTGCAACCTCAGCAGCGCGCGAGCAACGCCCCGCCGCCCATCCAAGGCGGCCAGGACATGGCCACCATGCTG
TTTCCTGCCTCCGTCGCCAACGGCATGTCGCTCGGTGCCGGCGGCAGCATGGGGTCGAGCTTCGGGTCCTAC
GGTCCGCGGTCTCTGTACAACATCAAATGGAACGGCAAAGTCAGCGGGTTCCTCGATCTCATCGCCGCCCGA
GCCGGCGTGTCCTGGCGCTACAACCCAACCGAGAAAAGGGTCGAGTTCTACTACCTGGACACTCGGACCTTC
CGCATCTACGCCTTCGACGACGTCAACACGGTGGACTCCACCGTGCGTTCCGGTATGACGACGGCCGCCGGC
ATCAGCGGGGACGGCTCCGGATCCACCGGACAGAATGGCAGCTCCGGCATCAGCGGCGACTCCGGCAGCAAG
CAGACCACCAGCTCGGAGCTGAAGACATCGATCCTCAGCGACATCGAGAACAGCATCAACTCGATGCTGACG
CCGAGCATGGGACGCATGTCGCTGTCGCGTGCCACGGGCACCCTGACCGTCACCGACCGTCCAGAAGTCCTC
AACCGTGTCCAGCAGTTGGTCAACCGAGAGAACGAGAGCATCACCAAGCAGGTGCTGCTGAACGTCAACGTG
CTCTCGGTCGCCCTGACCGACAAGGATCAACTGGGGATCGACTGGAACCTGGTCTACAAGTCGCTCAACAAC
AAGTGGGGCATCGGCCTGAAGAACACCATGCCGGGCATCGATCAAAGCGCGATCTCCGGCTCCGTGAGCATC
CTGGATACCGCCAACAGCGCCTGGGCAGGATCCAAGGCCATGGTCCAGGCGCTGGCCCAGCAGGGCCGCGTC
TCGACCGTCCGATCCCCGTCCGTGACCACGCTCAACCTCCAGTCGGCGCCGATCCAGATCGGCCGCTACGAC
AGCTACCTGGCCTCCAGCCAGATCTCCAACGTCGCCCAGGTCGGCAGTACCACCTCGCTGATCCCGGGCGCC
GTGACCAGCGGCTACAACATGAGCCTGCTGCCGTTCGTGATGGAAAGCGGCGAGATGCTGCTGAAGATCAAC
ATCAACATGACCTCCCGGCCGACGTTCGAAATGCAGACCAGCGGGGACTCCAAAGCCCAGTTCCCGAGCTAC
GACATACAACTGTTCGACCAGAAGGTACGTCTGCGCAGCGGCGAGACCTTGGTACTCTCCGGCTTCGACCAG
ACCACCGAGGACACCAACAAGGTCGGCACCGGCGACGCTGGGTTCTTCGGTCTTGGCGGCGGGCTGACCCGC
AATACCAAGCGCGAGGTCATCGTGGTGCTGATCACCCCCGTCGTGCTGGGCTGA
Translation:
MNESADRVEATADSASTIAAQVRNTRPDRRDTVVFSDKPWVSTKPLSVSHTLSSDCIVTWRPAGAASLQEAA
QEVINQCHMAVSITPDALNPAAFAVQPQQRASNAPPPIQGGQDMATMLFPASVANGMSLGAGGSMGSSFGSY
GPRSLYNIKWNGKVSGFLDLIAARAGVSWRYNPTEKRVEFYYLDTRTFRIYAFDDVNTVDSTVRSGMTTAAG
ISGDGSGSTGQNGSSGISGDSGSKQTTSSELKTSILSDIENSINSMLTPSMGRMSLSRATGTLTVTDRPEVL
NRVQQLVNRENESITKQVLLNVNVLSVALTDKDQLGIDWNLVYKSLNNKWGIGLKNTMPGIDQSAISGSVSI
LDTANSAWAGSKAMVQALAQQGRVSTVRSPSVTTLNLQSAPIQIGRYDSYLASSQISNVAQVGSTTSLIPGA
VTSGYNMSLLPFVMESGEMLLKININMTSRPTFEMQTSGDSKAQFPSYDIQLFDQKVRLRSGETLVLSGFDQ
TTEDTNKVGTGDAGFFGLGGGLTRNTKREVIVVLITPVVLG*
AnnotationID:684GeneID:675
AnnotatorUID: diggins
Modification Date/Time: 2006-01-29 08:08:09
Gene Name:pilN2
Confidence Code:1
GeneProduct:Type IV B pilus protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:secretin
Functional Category Confidence Code:5
COGs:
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAB12647.1| (AP002527) 40 pct identical(12 gaps) to gp:D88588_10[PilN of
           plasmid R64] [Escherichia coli]
          Length = 547

 Score =  313 bits (802), Expect = 2e-84
 Identities = 197/551 (35%), Positives = 304/551 (55%), Gaps = 46/551 (8%)

>gb|AAL18829.2| (AY258503) PilN [Escherichia coli]
          Length = 539

 Score =  311 bits (796), Expect = 9e-84
 Identities = 189/544 (34%), Positives = 301/544 (55%), Gaps = 38/544 (6%)






 
Structural Features:






   
Genomic Context:






   
Comment:


















    ReferenceID:14197




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18495




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38287




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:675_cdd.html


    		




 
URL:

















    ReferenceID:38288




  
Author/Investigator(s):
        Martin PR, Watson AA, McCaul TF, Mattick JS.





  
Title:
        Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa.




  
PubMed:
        7565110


MedLine:
        




    
Source:
Mol Microbiol. 1995 May;16(3):497-508.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7565110&query_hl=9&itool=pubmed_docsum









           
Homologs By Global Alignment 

           

       



     
Gene ID:675
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
42423
                           gb|AAP84210.1|
                           (AY273869) PilN2 [Pseudomonas aeruginosa]
                           545
                           98.34
                           98.53
                           5
                           2697.0
     

     
     
42424
                           gb|AAF81209.1|AF247502_4
                           (AF247502) PilNa [Salmonella enterica subsp. enterica serovar Dublin]
                           549
                           21.12
                           31.14
                           276
                           462.0
     

     
     
42425
                           gb|AAF14814.2|
                           (AF000001) PilNa [Salmonella typhi]
                           549
                           21.12
                           31.14
                           276
                           462.0
     

     






      

          
GC ORFID: 43677How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_RL085Glimmer Score: 

          
Start: 4542318Stop: 4543955

          
Length: 1638


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_RL085 pilN 540 Homolog: PilN / Type IVB pilus biosynthesis; Pathogenicity-related / E.coli K-12 / plasmid R721 (BAB12647) translation of RL085Homolog Bit Score 1046.0

          
Other Homologs:  psyr_15may02_Scaffold10_revised_gene1586 (ORF: BLASTX 553.0), t_RL085 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAACGAGTCGGCGGATCGTGTCGAAGCTACGGCAGATTCCGCGTCTACGATCGCAGCGCAGGTGCGCAAC 
ACCCGACCGGATCGGCGCGATACGGTGGTGTTCTCCGACAAACCCTGGGTCAGCACGAAACCCCTAAGCGTT 
TCGCACACCTTGTCCAGTGACTGCATCGTGACGTGGCGCCCTGCAGGCGCAGCGTCGCTGCAGGAGGCCGCC 
CAGGAAGTCATCAACCAATGCCACATGGCGGTCAGTATCACGCCCGACGCGCTGAACCCGGCCGCCTTCGCC 
GTGCAACCTCAGCAGCGCGCGAGCAACGCCCCGCCGCCCATCCAAGGCGGCCAGGACATGGCCACCATGCTG 
TTTCCTGCCTCCGTCGCCAACGGCATGTCGCTCGGTGCCGGCGGCAGCATGGGGTCGAGCTTCGGGTCCTAC 
GGTCCGCGGTCTCTGTACAACATCAAATGGAACGGCAAAGTCAGCGGGTTCCTCGATCTCATCGCCGCCCGA 
GCCGGCGTGTCCTGGCGCTACAACCCAACCGAGAAAAGGGTCGAGTTCTACTACCTGGACACTCGGACCTTC 
CGCATCTACGCCTTCGACGACGTCAACACGGTGGACTCCACCGTGCGTTCCGGTATGACGACGGCCGCCGGC 
ATCAGCGGGGACGGCTCCGGATCCACCGGACAGAATGGCAGCTCCGGCATCAGCGGCGACTCCGGCAGCAAG 
CAGACCACCAGCTCGGAGCTGAAGACATCGATCCTCAGCGACATCGAGAACAGCATCAACTCGATGCTGACG 
CCGAGCATGGGACGCATGTCGCTGTCGCGTGCCACGGGCACCCTGACCGTCACCGACCGTCCAGAAGTCCTC 
AACCGTGTCCAGCAGTTGGTCAACCGAGAGAACGAGAGCATCACCAAGCAGGTGCTGCTGAACGTCAACGTG 
CTCTCGGTCGCCCTGACCGACAAGGATCAACTGGGGATCGACTGGAACCTGGTCTACAAGTCGCTCAACAAC 
AAGTGGGGCATCGGCCTGAAGAACACCATGCCGGGCATCGATCAAAGCGCGATCTCCGGCTCCGTGAGCATC 
CTGGATACCGCCAACAGCGCCTGGGCAGGATCCAAGGCCATGGTCCAGGCGCTGGCCCAGCAGGGCCGCGTC 
TCGACCGTCCGATCCCCGTCCGTGACCACGCTCAACCTCCAGTCGGCGCCGATCCAGATCGGCCGCTACGAC 
AGCTACCTGGCCTCCAGCCAGATCTCCAACGTCGCCCAGGTCGGCAGTACCACCTCGCTGATCCCGGGCGCC 
GTGACCAGCGGCTACAACATGAGCCTGCTGCCGTTCGTGATGGAAAGCGGCGAGATGCTGCTGAAGATCAAC 
ATCAACATGACCTCCCGGCCGACGTTCGAAATGCAGACCAGCGGGGACTCCAAAGCCCAGTTCCCGAGCTAC 
GACATACAACTGTTCGACCAGAAGGTACGTCTGCGCAGCGGCGAGACCTTGGTACTCTCCGGCTTCGACCAG 
ACCACCGAGGACACCAACAAGGTCGGCACCGGCGACGCTGGGTTCTTCGGTCTTGGCGGCGGGCTGACCCGC 
AATACCAAGCGCGAGGTCATCGTGGTGCTGATCACCCCCGTCGTGCTGGGCTGA