ViewGene
Locus Name:
GeneID:
Location:

GeneID:674
TrivialName: PA14_64200
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64200
PAO1 Ortholog Locus:PA4854
Sequence Length:1608
Protein Length:535
Start: 5719711
Stop: 5721318
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08239.1|AE004898_10 (AE004898) phosphoribosylaminoimidazolecarboxamide formyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 533/535 (99%), Positives = 534/535 (99%)
Sequence:
ATGACCGACCAAACCACCCGCCTGCCCATCCGCCGCGCGCTGATCAGCGTTTCCGACAAGACCGGCGTCGTC
GACTTCGCCCGTGAGCTGGTCGCCCTCGGCGTGGAAATCCTTTCCACCGGCGGCACCTACAAGCTGCTCCGG
GACAACGGCATCTCTGCCGTGGAAGTGGCCGACTACACCGGCTTCCCGGAAATGATGGACGGTCGGGTGAAG
ACCCTGCACCCGAAGGTGCATGGCGGTATCCTCGGTCGCCGCGACCTCGACGGCGCGGTCATGGAGCAGCAC
GGCATCAAGCCGATCGACCTGGTGGCGGTCAACCTGTATCCCTTCGAGGCCACGGTGGTCAGGCCCGACTGC
GACCTGCCTACCGCCATCGAGAACATCGATATCGGCGGGCCGACCATGGTCCGTTCGGCGGCGAAGAACCAC
AAGGATGTCGCCATCGTGGTCAATGCCGGCGACTACGCCGCCGTGATCGAATCCCTCAAGGCCGGCGGCCTG
ACCTACGCCCAGCGTTTCGACCTGGCCCTCAAGGCGTTCGAGCACACCTCCGCCTACGACGGCATGATCGCC
AACTACCTGGGTACCATCGACCAGACCCGCGACACCCTCGGCACCGCCGACCGCGGCGCCTTCCCGCGCACC
TTCAACAGCCAGTTCGTCAAGGCCCAGGAAATGCGCTACGGCGAGAACCCGCACCAGAGCGCGGCGTTCTAC
GTCGAGGCGAAGAAGGGCGAGGCCAGCGTCTCCACCGCCATCCAGTTGCAAGGCAAGGAGTTGTCGTTCAAC
AACGTCGCCGACACCGACGCCGCCCTGGAATGCGTGAAGAGCTTCCTCAAGCCGGCCTGCGTGATCGTCAAG
CATGCCAACCCCTGCGGCGTCGCCGTGGTGCCGGAAGACGAAGGCGGCATCCGCAAGGCCTACGACCTGGCC
TACGCCACCGATAGCGAATCGGCGTTCGGCGGCATCATCGCCTTCAACCGCGAGCTGGACGGCGAAACCGCC
AAGGCCATCGTCGAGCGCCAGTTCGTCGAGGTGATCATCGCGCCGAAGATTTCCGCCGCCGCCCGCGAGGTG
GTCGCCGCCAAGGCCAACGTACGCCTGCTCGAATGCGGCGAATGGCCAGCCGAGCGCGCCCCGGGCTGGGAC
TTCAAGCGGGTCAACGGCGGCCTGCTGGTACAGAGCCGCGACATCGGCATGATCAAGGCCGAGGACCTGAAG
ATCGTCACTCGCCGCGCACCCACCGAGCAGGAGATCCACGACCTGATCTTCGCCTGGAAGGTGGCCAAGTTC
GTCAAGTCCAACGCCATCGTCTACGCCAGGAACCGCCAGACCGTCGGCGTCGGCGCCGGCCAGATGAGCCGG
GTCAACTCCGCACGGATCGCCGCGATCAAGGCCGAGCACGCCGGCCTGGAAGTGAAAGGCGCGGTGATGGCC
TCGGACGCCTTCTTCCCGTTCCGCGACGGCATCGACAACGCGGCCAAGGCCGGCATCACCGCGGTGATCCAG
CCGGGCGGCTCGATGCGCGACAGCGAAGTGATCGCGGCGGCCGACGAGGCGGATATCGCGATGGTGTTCACT
GGCATGCGCCATTTCCGCCATTGA
Translation:
MTDQTTRLPIRRALISVSDKTGVVDFARELVALGVEILSTGGTYKLLRDNGISAVEVADYTGFPEMMDGRVK
TLHPKVHGGILGRRDLDGAVMEQHGIKPIDLVAVNLYPFEATVVRPDCDLPTAIENIDIGGPTMVRSAAKNH
KDVAIVVNAGDYAAVIESLKAGGLTYAQRFDLALKAFEHTSAYDGMIANYLGTIDQTRDTLGTADRGAFPRT
FNSQFVKAQEMRYGENPHQSAAFYVEAKKGEASVSTAIQLQGKELSFNNVADTDAALECVKSFLKPACVIVK
HANPCGVAVVPEDEGGIRKAYDLAYATDSESAFGGIIAFNRELDGETAKAIVERQFVEVIIAPKISAAAREV
VAAKANVRLLECGEWPAERAPGWDFKRVNGGLLVQSRDIGMIKAEDLKIVTRRAPTEQEIHDLIFAWKVAKF
VKSNAIVYARNRQTVGVGAGQMSRVNSARIAAIKAEHAGLEVKGAVMASDAFFPFRDGIDNAAKAGITAVIQ
PGGSMRDSEVIAAADEADIAMVFTGMRHFRH*
AnnotationID:683GeneID:674
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 06:06:19
Gene Name:purH
Confidence Code:2
GeneProduct:phosphoribosylaminoimidazolecarboxamide transferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:IMP cyclohydrolase
Functional Category Confidence Code:5
COGs:pfam01808,cd01421
Secondary Functional Category(ies):central intermediary metabolism
EC Number:2.1.2.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70391.1|AE016680_11 (AE016792) phosphoribosylaminoimidazolecarboxamide
           formyltransferase/IMP cyclohydrolase [Pseudomonas putida
           KT2440]
          Length = 535

 Score =  962 bits (2488), Expect = 0.0
 Identities = 477/535 (89%), Positives = 509/535 (95%)

>gb|AAO58295.1| (AE016873) phosphoribosylaminoimidazolecarboxamide
           formyltransferase/IMP cyclohydrolase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 534

 Score =  920 bits (2377), Expect = 0.0
 Identities = 458/535 (85%), Positives = 496/535 (92%), Gaps = 1/535 (0%)

>gb|AAN83392.1|AE016770_192 (AE016770) Purine biosynthesis protein PurH;
           Phosphoribosylaminoimidazolecarboxamide
           formyltransferase; IMP cyclohydrolase [Escherichia coli
           CFT073]
          Length = 529

 Score =  721 bits (1861), Expect = 0.0
 Identities = 366/531 (68%), Positives = 420/531 (79%), Gaps = 7/531 (1%)








 
Structural Features:
pfam01808, AICARFT_IMPCHas, AICARFT/IMPCHase bienzyme.
CD-Length = 315 residues, 100.0% aligned
Score =  384 bits (987), Expect = 2e-107 
Identities = 181/330 (54%), Positives = 216/330 (65%), Gaps = 17/330 (5%)

cd01421, IMPCH, Inosine monophosphate cyclohydrolase domain. 
CD-Length = 187 residues, 100.0% aligned
Score =  289 bits (741), Expect = 6e-79







   
Genomic Context:






   
Comment:


















    ReferenceID:1351




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18493




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35180




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:674_cdd.html


    		




 
URL:

















    ReferenceID:35971




  
Author/Investigator(s):
        Aiba A, Mizobuchi K.





  
Title:
        Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli.




  
PubMed:
        2687276


MedLine:
        




    
Source:
J Biol Chem. 1989 Dec 15;264(35):21239-46.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2687276

















    ReferenceID:35972




  
Author/Investigator(s):
        Chopra AK, Peterson JW, Prasad R.





  
Title:
        Nucleotide sequence analysis of purH and purD genes from Salmonella typhimurium.




  
PubMed:
        1954258


MedLine:
        




    
Source:
Biochim Biophys Acta. 1991 Nov 11;1090(3):351-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1954258









           
Homologs By Global Alignment 

           

       



     
Gene ID:674
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1138
                           PA4854_tr
                           translation of PA4854
                           535
                           99.62
                           99.81
                           0
                           2706.0
     

     
     
42291
                           gb|AAG08239.1|AE004898_10
                           (AE004898) phosphoribosylaminoimidazolecarboxamide formyltransferase [Pseudomonas aeruginosa PAO1]
                           535
                           99.62
                           99.81
                           0
                           2706.0
     

     
     
42292
                           gb|AAN70391.1|AE016680_11
                           (AE016792) phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase [Pseudomonas putida KT2440]
                           535
                           89.15
                           95.14
                           0
                           2488.0
     

     
     
42293
                           gb|AAO58295.1|
                           (AE016873) phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase [Pseudomonas syringae pv. tomato str. DC3000]
                           535
                           85.60
                           92.71
                           1
                           2379.0
     

     
     
42304
                           dbj|BAC61159.1|
                           (AP005083) phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase [Vibrio parahaemolyticus]
                           538
                           68.95
                           78.06
                           11
                           1855.0
     

     






      

          
GC ORFID: 43676How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4854Glimmer Score: 

          
Start: 4983391Stop: 4984998

          
Length: 1608


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4854 translation of PA4854Homolog Bit Score 1046.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5776 (ORF: BLASTX 922.0), t_PA4854 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGACCAAACCACCCGCCTGCCCATCCGCCGCGCGCTGATCAGCGTTTCCGACAAGACCGGCGTCGTC 
GACTTCGCCCGTGAGCTGGTCGCCCTCGGCGTGGAAATCCTTTCCACCGGCGGCACCTACAAGCTGCTCCGG 
GACAACGGCATCTCTGCCGTGGAAGTGGCCGACTACACCGGCTTCCCGGAAATGATGGACGGTCGGGTGAAG 
ACCCTGCACCCGAAGGTGCATGGCGGTATCCTCGGTCGCCGCGACCTCGACGGCGCGGTCATGGAGCAGCAC 
GGCATCAAGCCGATCGACCTGGTGGCGGTCAACCTGTATCCCTTCGAGGCCACGGTGGTCAGGCCCGACTGC 
GACCTGCCTACCGCCATCGAGAACATCGATATCGGCGGGCCGACCATGGTCCGTTCGGCGGCGAAGAACCAC 
AAGGATGTCGCCATCGTGGTCAATGCCGGCGACTACGCCGCCGTGATCGAATCCCTCAAGGCCGGCGGCCTG 
ACCTACGCCCAGCGTTTCGACCTGGCCCTCAAGGCGTTCGAGCACACCTCCGCCTACGACGGCATGATCGCC 
AACTACCTGGGTACCATCGACCAGACCCGCGACACCCTCGGCACCGCCGACCGCGGCGCCTTCCCGCGCACC 
TTCAACAGCCAGTTCGTCAAGGCCCAGGAAATGCGCTACGGCGAGAACCCGCACCAGAGCGCGGCGTTCTAC 
GTCGAGGCGAAGAAGGGCGAGGCCAGCGTCTCCACCGCCATCCAGTTGCAAGGCAAGGAGTTGTCGTTCAAC 
AACGTCGCCGACACCGACGCCGCCCTGGAATGCGTGAAGAGCTTCCTCAAGCCGGCCTGCGTGATCGTCAAG 
CATGCCAACCCCTGCGGCGTCGCCGTGGTGCCGGAAGACGAAGGCGGCATCCGCAAGGCCTACGACCTGGCC 
TACGCCACCGATAGCGAATCGGCGTTCGGCGGCATCATCGCCTTCAACCGCGAGCTGGACGGCGAAACCGCC 
AAGGCCATCGTCGAGCGCCAGTTCGTCGAGGTGATCATCGCGCCGAAGATTTCCGCCGCCGCCCGCGAGGTG 
GTCGCCGCCAAGGCCAACGTACGCCTGCTCGAATGCGGCGAATGGCCAGCCGAGCGCGCCCCGGGCTGGGAC 
TTCAAGCGGGTCAACGGCGGCCTGCTGGTACAGAGCCGCGACATCGGCATGATCAAGGCCGAGGACCTGAAG 
ATCGTCACTCGCCGCGCACCCACCGAGCAGGAGATCCACGACCTGATCTTCGCCTGGAAGGTGGCCAAGTTC 
GTCAAGTCCAACGCCATCGTCTACGCCAGGAACCGCCAGACCGTCGGCGTCGGCGCCGGCCAGATGAGCCGG 
GTCAACTCCGCACGGATCGCCGCGATCAAGGCCGAGCACGCCGGCCTGGAAGTGAAAGGCGCGGTGATGGCC 
TCGGACGCCTTCTTCCCGTTCCGCGACGGCATCGACAACGCGGCCAAGGCCGGCATCACCGCGGTGATCCAG 
CCGGGCGGCTCGATGCGCGACAGCGAAGTGATCGCGGCGGCCGACGAGGCGGATATCGCGATGGTGTTCACT 
GGCATGCGCCATTTCCGCCATTGA