ViewGene
Locus Name:
GeneID:
Location:

GeneID:672
TrivialName: PA14_68730
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_68730
PAO1 Ortholog Locus:PA5203
Sequence Length:1584
Protein Length:527
Start: 6128743
Stop: 6130326
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08588.1|AE004933_4 (AE004933) glutamate--cysteine ligase [Pseudomonas aeruginosa PAO1]
Identities = 525/527 (99%), Positives = 527/527 (100%)
Sequence:
TTGAGCGATCTTCTCTCCCGCCGCCTGGCTCTGCTCGGCGCCGCAGCCAACCTGCCCCTGCTGACCGAGTGC
CTGCATGGCATCGAGCGCGAATGCCTGCGAGTCGATAGCGACGGCAAGCTGGCGCTCACCCCCCACCCCCGG
GCCCTGGGTTCGACCCTGACCCATCCGCAGATCACTACCGACTACTCCGAGGCCTTGCTGGAGTTCATCACG
CCCACGGAAACCGACGTCGCCGACACTCTGGGCGATCTCGAGCGCATCCACCGCTTCGCCAGTTCGAAACTG
GACGGCGAATACCTGTGGAGCCCATCGATGCCCTGCGAGCTGCCGGACGAGGAGAGCATCCCGATCGCCCGC
TACGGCAGCTCGCTGATCGGCCGCCTGAAGTACGTCTACCGCAAGGGCCTGGCGCTGCGCTACGGCAAGACC
ATGCAGTGCATCGCCGGCATCCACTACAACTTCTCCCTGCCGGAGAGGCTCTGGCCGCTGCTGCGCCAGGCC
GAAGGCAGCGAGCTATCGGAACGCGACTACCAGTCCGCCGCCTACATCGCCCTGATTCGCAATTTCCGCCGC
TACAGCTGGCTGCTGATGTACCTGTTCGGCGCCTCGCCGGCCCTCGACGCCGGCTTCCTGCGTGGCCGCCCG
AGCCAGTTGGAGCGCCTCGACGAACACACGCTCTACCTGCCCTACGCCACCAGCCTGCGGATGAGCGACCTG
GGCTACCAGAACAACGCCCAGGCCGGCCTGACGCCCTGCTACAACGACCTGCAGAGCTACATCGACAGCCTG
CGCCAGGCGGTCAGCACGCCCTACCCGCCCTACGAGAAGGTCGGCACCAAGCAGGATGGCGAATGGGTGCAA
CTGAACACCAACATCCTGCAGATCGAGAACGAGTACTACTCGAGCATCCGGCCAAAGCGCGTCACCTACACC
GGCGAGCGTCCGGTGCAGGCCCTGGCCGCTCGCGGCGTGCAGTACGTGGAAGTGCGCTGCCTGGACATCAAC
CCGTTCCTGCCGCTGGGCATCGACCTGGACGAGGCGCGCTTCCTCGACGCCTTCCTGCTGTTCTGCGCGTTC
AGCGACAGCCCGCTGCTGAACGGCGAGTGCAGCGACGCCACCGACAACTTCCTCGCCGTGGTCAAGGAAGGC
AGGCGGCCGGGCCTGCAATTGCAACGTCGCGGCCAGCCGGTGGAATTGAAGGTCTGGGCCAACGAGCTACTC
GAACGAATCGCCGATACCGCGGCGCTGCTCGACCGCGCACGCGGCGGCGAAGCCCACGCGGCGGCGCTGGCA
GCGCAGCGGGCTAAGGTGGCCGATGCGGAACTGACGCCTTCGGCGCAGGTGCTGAAGGTCATGCGCGAGCGC
GGCGAGAGCTTCGAAGCGTTCTCCCTGCGCCAGAGCCGCGAACACGCGGAGTATTTCCGCCAGCATCCGCTG
GCCGCCGAAGAGCAGGCACGCTTCGAGAAGATGGCCAGCGACTCCCTGGCCGAACAGACGGAGCTGGAACGC
GACCAGGATGGCGACTTCGATACCTTCGTCGCCGCCTACCAGGCGAGCATCCTCGGCCTGATCAGCAACTGA
Translation:
MSDLLSRRLALLGAAANLPLLTECLHGIERECLRVDSDGKLALTPHPRALGSTLTHPQITTDYSEALLEFIT
PTETDVADTLGDLERIHRFASSKLDGEYLWSPSMPCELPDEESIPIARYGSSLIGRLKYVYRKGLALRYGKT
MQCIAGIHYNFSLPERLWPLLRQAEGSELSERDYQSAAYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRP
SQLERLDEHTLYLPYATSLRMSDLGYQNNAQAGLTPCYNDLQSYIDSLRQAVSTPYPPYEKVGTKQDGEWVQ
LNTNILQIENEYYSSIRPKRVTYTGERPVQALAARGVQYVEVRCLDINPFLPLGIDLDEARFLDAFLLFCAF
SDSPLLNGECSDATDNFLAVVKEGRRPGLQLQRRGQPVELKVWANELLERIADTAALLDRARGGEAHAAALA
AQRAKVADAELTPSAQVLKVMRERGESFEAFSLRQSREHAEYFRQHPLAAEEQARFEKMASDSLAEQTELER
DQDGDFDTFVAAYQASILGLISN*
AnnotationID:681GeneID:672
AnnotatorUID: diggins
Modification Date/Time: 2006-01-21 22:10:21
Gene Name:gshA
Confidence Code:2
GeneProduct:glutamate--cysteine ligase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2918, pfam04262
Secondary Functional Category(ies):Amino acid biosynthesis and metabolism
EC Number:6.3.2.2
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65875.1|AE016215_10 (AE016774) glutamate--cysteine ligase [Pseudomonas putida KT2440]
          Length = 525

 Score =  782 bits (2019), Expect = 0.0
 Identities = 394/527 (74%), Positives = 437/527 (82%), Gaps = 3/527 (0%)

>gb|AAO53870.1| (AE016857) glutamate--cysteine ligase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 529

 Score =  741 bits (1913), Expect = 0.0
 Identities = 375/527 (71%), Positives = 427/527 (81%), Gaps = 3/527 (0%)

>dbj|BAA16555.1| (D90892) glutamate--cysteine ligase (EC 6.3.2.2) [Escherichia coli]
          Length = 518

 Score =  384 bits (986), Expect = e-106
 Identities = 213/489 (43%), Positives = 304/489 (62%), Gaps = 19/489 (3%)







 
Structural Features:
COG2918, GshA, Gamma-glutamylcysteine synthetase 
[Coenzyme metabolism].
CD-Length = 518 residues, 100.0% aligned
Score =  712 bits (1838), Expect = 0.0
Identities = 271/522 (51%), Positives = 335/522 (64%), Gaps = 8/522 (1%)

pfam04262, Glutamate-cysteine ligase.
CD-Length = 518 residues, 100.0% aligned
Score =  712 bits (1838), Expect = 0.0
Identities = 248/377 (65%), Positives = 289/377 (76%), Gaps = 1/377 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14195




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17032




  
Author/Investigator(s):
        





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:
NCBI - Conserved Domain Search (BLASTP).






    
Reference Type:Blast (text/plain)







    
Data:PA5203_CDS.cgi


    		




 
URL:

















    ReferenceID:18489




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38254




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:672_cdd.html


    		




 
URL:

















    ReferenceID:38255




  
Author/Investigator(s):
        Watanabe K, Yamano Y, Murata K, Kimura A





  
Title:
        The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.




  
PubMed:
        2872655


MedLine:
        




    
Source:
Nucleic Acids Res. 1986 Jun 11;14(11):4393-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=2872655









           
Homologs By Global Alignment 

           

       



     
Gene ID:672
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1132
                           PA5203_tr
                           translation of PA5203
                           527
                           99.62
                           99.99
                           0
                           2707.0
     

     
     
42218
                           gb|AAG08588.1|AE004933_4
                           (AE004933) glutamate--cysteine ligase [Pseudomonas aeruginosa PAO1]
                           527
                           99.43
                           99.99
                           0
                           2705.0
     

     
     
42219
                           gb|AAN65875.1|AE016215_10
                           (AE016774) glutamate--cysteine ligase [Pseudomonas putida KT2440]
                           528
                           74.43
                           82.76
                           4
                           2020.0
     

     
     
42220
                           gb|AAO53870.1|
                           (AE016857) glutamate--cysteine ligase [Pseudomonas syringae pv. tomato str. DC3000]
                           530
                           71.13
                           80.94
                           4
                           1918.0
     

     
     
42221
                           gb|AAQ82441.1|
                           (AY374326) unknown [Pseudomonas syringae pv. syringae]
                           536
                           69.02
                           80.03
                           10
                           1883.0
     

     






      

          
GC ORFID: 43674How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5203Glimmer Score: 

          
Start: 5392429Stop: 5394012

          
Length: 1584


          
Start Codon: TTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5203 translation of PA5203Homolog Bit Score 1047.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3909 (ORF: BLASTX 724.0), t_PA5203 (ORF: GLIMMER)

          
GC ORF Sequence

           
TTGAGCGATCTTCTCTCCCGCCGCCTGGCTCTGCTCGGCGCCGCAGCCAACCTGCCCCTGCTGACCGAGTGC 
CTGCATGGCATCGAGCGCGAATGCCTGCGAGTCGATAGCGACGGCAAGCTGGCGCTCACCCCCCACCCCCGG 
GCCCTGGGTTCGACCCTGACCCATCCGCAGATCACTACCGACTACTCCGAGGCCTTGCTGGAGTTCATCACG 
CCCACGGAAACCGACGTCGCCGACACTCTGGGCGATCTCGAGCGCATCCACCGCTTCGCCAGTTCGAAACTG 
GACGGCGAATACCTGTGGAGCCCATCGATGCCCTGCGAGCTGCCGGACGAGGAGAGCATCCCGATCGCCCGC 
TACGGCAGCTCGCTGATCGGCCGCCTGAAGTACGTCTACCGCAAGGGCCTGGCGCTGCGCTACGGCAAGACC 
ATGCAGTGCATCGCCGGCATCCACTACAACTTCTCCCTGCCGGAGAGGCTCTGGCCGCTGCTGCGCCAGGCC 
GAAGGCAGCGAGCTATCGGAACGCGACTACCAGTCCGCCGCCTACATCGCCCTGATTCGCAATTTCCGCCGC 
TACAGCTGGCTGCTGATGTACCTGTTCGGCGCCTCGCCGGCCCTCGACGCCGGCTTCCTGCGTGGCCGCCCG 
AGCCAGTTGGAGCGCCTCGACGAACACACGCTCTACCTGCCCTACGCCACCAGCCTGCGGATGAGCGACCTG 
GGCTACCAGAACAACGCCCAGGCCGGCCTGACGCCCTGCTACAACGACCTGCAGAGCTACATCGACAGCCTG 
CGCCAGGCGGTCAGCACGCCCTACCCGCCCTACGAGAAGGTCGGCACCAAGCAGGATGGCGAATGGGTGCAA 
CTGAACACCAACATCCTGCAGATCGAGAACGAGTACTACTCGAGCATCCGGCCAAAGCGCGTCACCTACACC 
GGCGAGCGTCCGGTGCAGGCCCTGGCCGCTCGCGGCGTGCAGTACGTGGAAGTGCGCTGCCTGGACATCAAC 
CCGTTCCTGCCGCTGGGCATCGACCTGGACGAGGCGCGCTTCCTCGACGCCTTCCTGCTGTTCTGCGCGTTC 
AGCGACAGCCCGCTGCTGAACGGCGAGTGCAGCGACGCCACCGACAACTTCCTCGCCGTGGTCAAGGAAGGC 
AGGCGGCCGGGCCTGCAATTGCAACGTCGCGGCCAGCCGGTGGAATTGAAGGTCTGGGCCAACGAGCTACTC 
GAACGAATCGCCGATACCGCGGCGCTGCTCGACCGCGCACGCGGCGGCGAAGCCCACGCGGCGGCGCTGGCA 
GCGCAGCGGGCTAAGGTGGCCGATGCGGAACTGACGCCTTCGGCGCAGGTGCTGAAGGTCATGCGCGAGCGC 
GGCGAGAGCTTCGAAGCGTTCTCCCTGCGCCAGAGCCGCGAACACGCGGAGTATTTCCGCCAGCATCCGCTG 
GCCGCCGAAGAGCAGGCACGCTTCGAGAAGATGGCCAGCGACTCCCTGGCCGAACAGACGGAGCTGGAACGC 
GACCAGGATGGCGACTTCGATACCTTCGTCGCCGCCTACCAGGCGAGCATCCTCGGCCTGATCAGCAACTGA