ViewGene
Locus Name:
GeneID:
Location:

GeneID:671
TrivialName: PA14_66310
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66310
PAO1 Ortholog Locus:PA5016
Sequence Length:1644
Protein Length:547
Start: 5910346
Stop: 5911989
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08401.1|AE004914_2 (AE004914) dihydrolipoamide acetyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 546/547 (99%), Positives = 547/547 (100%)
Sequence:
GTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTGCTGGTCAAGCCC
GGCGACAAGGTCGAGGCCGATCAGAGCCTGCTGACCCTGGAATCCGACAAGGCCAGCATGGAAATCCCCAGT
CCCAAGGCCGGGGTAGTGAAAAGCATCAAGGCGAAGGTCGGCGACACCTTGAAAGAAGGTGACGAAATCCTC
GAGCTGGAAGTGGAAGGCGGCGAACAGCCTGCCGAAGCCAAGGCCGAGGCAGCGCCCGCCCAACCGGAAGCG
CCGAAAGCCGAAGCGCCTGCTCCCGCCCCGAGCGAGAGCAAGCCGGCCGCCCCCGCCGCAGCCAGTGTCCAG
GACATCAAGGTCCCGGACATCGGCTCGGCCGGCAAGGCCAACGTCATCGAAGTGATGGTCAAGGCTGGCGAC
ACGGTCGAGGCCGACCAGTCGCTGATCACCCTGGAATCCGACAAGGCCAGCATGGAGATCCCTTCGCCGGCC
TCCGGCGTGGTGGAAAGCGTCTCGATCAAGGTCGGTGACGAAGTCGGCACCGGCGACCTGATCCTCAAGCTG
AAGGTGGAAGGCGCCGCtccGGCAGCCGAAGAGCAGCCGGCAGCCGCTCCGGCCCAGGCCGCGGCGCCCGCC
GCCGAGCAGAAGCCCGCCGCGGCGGCCCCTGCGCCAGCCAAGGCCGATACCCCGGCTCCGGTCGGCGCACCC
AGCCGCGACGGCGCCAAGGTCCACGCCGGCCCGGCGGTGCGCATGCTGGCGCGCGAGTTCGGCGTCGAGCTG
AGCGAAGTGAAAGCCAGCGGTCCCAAGGGCCGCATCCTCAAGGAAGACGTCCAGGTCTTCGTCAAGGAGCAA
CTGCAGCGCGCCAAGTCCGGCGGTGCCGGCGCCACCGGCGGAGCCGGCATCCCGCCGATCCCGGAAGTCGAC
TTCAGCAAGTTCGGCGAAGTGGAAGAAGTGGCGATGACCCGCCTGATGCAGGTCGGCGCCGCCAACCTGCAT
CGCAGCTGGCTGAACGTGCCGCACGTGACCCAGTTCGACCAGTCGGACATCACCGACATGGAAGCCTTCCGC
GTTGCCCAGAAGGCCGCGGCGGAGAAGGCCGGGGTCAAGCTGACCGTACTGCCGATCCTGCTCAAGGCCTGC
GCCCACCTGCTCAAGGAACTGCCGGACTTCAACAGTTCGCTGGCCCCCAGCGGCAAGGCGCTGATTCGCAAG
AAGTACGTACACATCGGCTTCGCCGTGGACACTCCGGACGGCCTGCTGGTCCCGGTGATCCGCGATGTCGAC
CGGAAGAGCCTCCTGCAACTGGCCGCCGAGGCCGCCGAGCTGGCCGACAAGGCCCGCAACAAGAAGCTCTCG
GCCGATGCCATGCAGGGCGCCTGCTTCACCATCTCCAGTCTCGGCCACATCGGCGGCACCGGCTTCACGCCG
ATCGTCAACGCGCCGGAAGTGGCGATCCTCGGTGTGTCCAAGGCGACCATGCAGCCGGTATGGGACGGCAAG
GCCTTCCAGCCGCGCCTGATGCTGCCGCTGTCGCTGTCCTACGACCATCGCGTGATCAACGGCGCCGCCGCG
GCGCGCTTCACCAAGCGCCTGGGCGAGCTGCTGGCGGACATCCGCACCCTGCTCCTGTAA
Translation:
MSELIRVPDIGNGEGEVIELLVKPGDKVEADQSLLTLESDKASMEIPSPKAGVVKSIKAKVGDTLKEGDEIL
ELEVEGGEQPAEAKAEAAPAQPEAPKAEAPAPAPSESKPAAPAAASVQDIKVPDIGSAGKANVIEVMVKAGD
TVEADQSLITLESDKASMEIPSPASGVVESVSIKVGDEVGTGDLILKLKVEGAAPAAEEQPAAAPAQAAAPA
AEQKPAAAAPAPAKADTPAPVGAPSRDGAKVHAGPAVRMLAREFGVELSEVKASGPKGRILKEDVQVFVKEQ
LQRAKSGGAGATGGAGIPPIPEVDFSKFGEVEEVAMTRLMQVGAANLHRSWLNVPHVTQFDQSDITDMEAFR
VAQKAAAEKAGVKLTVLPILLKACAHLLKELPDFNSSLAPSGKALIRKKYVHIGFAVDTPDGLLVPVIRDVD
RKSLLQLAAEAAELADKARNKKLSADAMQGACFTISSLGHIGGTGFTPIVNAPEVAILGVSKATMQPVWDGK
AFQPRLMLPLSLSYDHRVINGAAAARFTKRLGELLADIRTLLL*
AnnotationID:680GeneID:671
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 10:10:00
Gene Name:aceF
Confidence Code:1
GeneProduct:dihydrolipoamide acetyltransferase
Cell Localization:()
Synonyms:aceB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:pyruvate dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase
Functional Category Confidence Code:5
COGs:COG0508,pfam00198
Secondary Functional Category(ies):carbon compond metabolism
EC Number:2.3.1.12
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58434.1| (AE016874) pyruvate dehydrogenase complex, E2 component,
           dihydrolipoamide acetyltransferase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 548

 Score =  824 bits (2128), Expect = 0.0
 Identities = 430/550 (78%), Positives = 482/550 (87%), Gaps = 5/550 (0%)

>gb|AAN65969.1|AE016225_2 (AE016775) pyruvate dehydrogenase, dihydrolipoamide
           acetyltransferase component [Pseudomonas putida KT2440]
          Length = 546

 Score =  821 bits (2121), Expect = 0.0
 Identities = 437/551 (79%), Positives = 471/551 (85%), Gaps = 9/551 (1%)

>emb|CAA30987.1| (X12455) dihydrolipoyltransacetylase (AA 1 - 638) [Azotobacter
           vinelandii]
          Length = 638

 Score =  751 bits (1940), Expect = 0.0
 Identities = 397/548 (72%), Positives = 453/548 (82%), Gaps = 28/548 (5%)






 
Structural Features:
COG0508, AceF, Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide 
acyltransferase (E2) component, and related enzymes 
[Energy production and conversion]
CD-Length = 404 residues,  99.0% aligned
Score =  311 bits (797), Expect = 1e-85
Identities = 182/428 (42%), Positives = 250/428 (58%), Gaps = 29/428 (6%)

pfam00198, 2-oxoacid_dh, 2-oxoacid dehydrogenases acyltransferase 
(catalytic domain). 
CD-Length = 232 residues,  99.6% aligned
Score =  275 bits (704), Expect = 1e-74
Identities = 113/231 (48%), Positives = 147/231 (63%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14193




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18487




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35045




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:671_cdd.html


    		




 
URL:

















    ReferenceID:35865




  
Author/Investigator(s):
        Rae JL, Cutfield JF, Lamont IL.





  
Title:
        Sequences and expression of pyruvate dehydrogenase genes from Pseudomonas aeruginosa.




  
PubMed:
        9171401


MedLine:
        




    
Source:
J Bacteriol. 1997 Jun;179(11):3561-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9171401

















    ReferenceID:35866




  
Author/Investigator(s):
        Byrne C, Stokes HW, Ward KA.





  
Title:
        Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli.




  
PubMed:
        3060852


MedLine:
        




    
Source:
Nucleic Acids Res. 1988 Nov 25;16(22):10924.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3060852

















    ReferenceID:35867




  
Author/Investigator(s):
        Stephens PE, Darlison MG, Lewis HM, Guest JR.





  
Title:
        The pyruvate dehydrogenase complex of Escherichia coli K12. Nucleotide sequence encoding the dihydrolipoamide acetyltransferase component.




  
PubMed:
        6345153


MedLine:
        




    
Source:
Eur J Biochem. 1983 Jul 1;133(3):481-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6345153









           
Homologs By Global Alignment 

           

       



     
Gene ID:671
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1131
                           PA5016_tr
                           translation of PA5016
                           547
                           99.81
                           99.99
                           0
                           2708.0
     

     
     
42155
                           gb|AAG08401.1|AE004914_2
                           (AE004914) dihydrolipoamide acetyltransferase [Pseudomonas aeruginosa PAO1]
                           547
                           99.63
                           99.99
                           0
                           2705.0
     

     
     
42156
                           gb|AAC45354.1|
                           (U47920) dihydrolipoamide acetyltransferase [Pseudomonas aeruginosa]
                           547
                           97.98
                           98.72
                           1
                           2646.0
     

     
     
42158
                           gb|AAN65969.1|AE016225_2
                           (AE016775) pyruvate dehydrogenase, dihydrolipoamide acetyltransferase component [Pseudomonas putida KT2440]
                           557
                           79.35
                           85.63
                           21
                           2135.0
     

     
     
42157
                           gb|AAO58434.1|
                           (AE016874) pyruvate dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           552
                           78.44
                           88.04
                           9
                           2139.0
     

     






      

          
GC ORFID: 43673How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5016Glimmer Score: 

          
Start: 5174029Stop: 5175672

          
Length: 1644


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5016 translation of PA5016Homolog Bit Score 1047.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3685 (ORF: BLASTX 830.0), t_PA5016 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAGCGAACTCATTCGCGTACCCGACATCGGCAACGGTGAGGGTGAAGTCATCGAGCTGCTGGTCAAGCCC 
GGCGACAAGGTCGAGGCCGATCAGAGCCTGCTGACCCTGGAATCCGACAAGGCCAGCATGGAAATCCCCAGT 
CCCAAGGCCGGGGTAGTGAAAAGCATCAAGGCGAAGGTCGGCGACACCTTGAAAGAAGGTGACGAAATCCTC 
GAGCTGGAAGTGGAAGGCGGCGAACAGCCTGCCGAAGCCAAGGCCGAGGCAGCGCCCGCCCAACCGGAAGCG 
CCGAAAGCCGAAGCGCCTGCTCCCGCCCCGAGCGAGAGCAAGCCGGCCGCCCCCGCCGCAGCCAGTGTCCAG 
GACATCAAGGTCCCGGACATCGGCTCGGCCGGCAAGGCCAACGTCATCGAAGTGATGGTCAAGGCTGGCGAC 
ACGGTCGAGGCCGACCAGTCGCTGATCACCCTGGAATCCGACAAGGCCAGCATGGAGATCCCTTCGCCGGCC 
TCCGGCGTGGTGGAAAGCGTCTCGATCAAGGTCGGTGACGAAGTCGGCACCGGCGACCTGATCCTCAAGCTG 
AAGGTGGAAGGCGCCGCtccGGCAGCCGAAGAGCAGCCGGCAGCCGCTCCGGCCCAGGCCGCGGCGCCCGCC 
GCCGAGCAGAAGCCCGCCGCGGCGGCCCCTGCGCCAGCCAAGGCCGATACCCCGGCTCCGGTCGGCGCACCC 
AGCCGCGACGGCGCCAAGGTCCACGCCGGCCCGGCGGTGCGCATGCTGGCGCGCGAGTTCGGCGTCGAGCTG 
AGCGAAGTGAAAGCCAGCGGTCCCAAGGGCCGCATCCTCAAGGAAGACGTCCAGGTCTTCGTCAAGGAGCAA 
CTGCAGCGCGCCAAGTCCGGCGGTGCCGGCGCCACCGGCGGAGCCGGCATCCCGCCGATCCCGGAAGTCGAC 
TTCAGCAAGTTCGGCGAAGTGGAAGAAGTGGCGATGACCCGCCTGATGCAGGTCGGCGCCGCCAACCTGCAT 
CGCAGCTGGCTGAACGTGCCGCACGTGACCCAGTTCGACCAGTCGGACATCACCGACATGGAAGCCTTCCGC 
GTTGCCCAGAAGGCCGCGGCGGAGAAGGCCGGGGTCAAGCTGACCGTACTGCCGATCCTGCTCAAGGCCTGC 
GCCCACCTGCTCAAGGAACTGCCGGACTTCAACAGTTCGCTGGCCCCCAGCGGCAAGGCGCTGATTCGCAAG 
AAGTACGTACACATCGGCTTCGCCGTGGACACTCCGGACGGCCTGCTGGTCCCGGTGATCCGCGATGTCGAC 
CGGAAGAGCCTCCTGCAACTGGCCGCCGAGGCCGCCGAGCTGGCCGACAAGGCCCGCAACAAGAAGCTCTCG 
GCCGATGCCATGCAGGGCGCCTGCTTCACCATCTCCAGTCTCGGCCACATCGGCGGCACCGGCTTCACGCCG 
ATCGTCAACGCGCCGGAAGTGGCGATCCTCGGTGTGTCCAAGGCGACCATGCAGCCGGTATGGGACGGCAAG 
GCCTTCCAGCCGCGCCTGATGCTGCCGCTGTCGCTGTCCTACGACCATCGCGTGATCAACGGCGCCGCCGCG 
GCGCGCTTCACCAAGCGCCTGGGCGAGCTGCTGGCGGACATCCGCACCCTGCTCCTGTAA