ViewGene
Locus Name:
GeneID:
Location:

GeneID:67
TrivialName: PA14_16820
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16820
PAO1 Ortholog Locus:PA3676
Sequence Length:3078
Protein Length:1025
Start: 1438753
Stop: 1441830
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07064.1|AE004787_4 (AE004787) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
Identities = 1023/1025 (99%), Positives = 1024/1025 (99%)
Sequence:
ATGTCCTTCAACCTTTCCGCCTGGGCGTTGCAGAATCGCCAGATCGTCCTGTACCTGATGATCCTGCTTGGC
GCGGTTGGCGCGCTGTCCTACAGCAAGCTGGGGCAGAGCGAAGACCCGCCGTTCACCTTCAAGGCCATGGTG
GTGCAGACCAACTGGTCGGGTGCCAGCGCCGAAGAGGTGGCCCGGCAGGTCACCGAGCGTATCGAGAAGAAG
CTGATGGAAACCGGCGACTACGATCGCATCGTGTCCTTCTCCCGCCCCGGCGTCTCGCAGGTGACCTTCATG
GCCCGCGAGGACATCCATTCCAGCGAGATCCCCGAACTCTGGTACCAGATCCGCAAGAAGATCAGCGACATT
CGCGCCACCTTGCCGCAAAGCATCCAGGGCCCGTTCTTCAACGACGAGTTCGGCACCACCTACGGCAACATC
TATGCGCTCACCGGCAAGGGCTTCGACTATGCGGTGATGAAGGACTATGCCGACCGCCTGCAACTGCAATTG
CAGCGGATCAGGAACGTCGGCAAGGTCGAACTGATCGGCCTGCAGGACGAGAAGATCTGGATCGACCTGTCC
AACACCAAGCTGGCCACCCTCGGCCTGCCCCTGGCGGCGGTGCAGAAGGCGCTGGAGGAACAGAACGCGGTG
GCCTCCTCCGGGTTCTTCGAGACCGCCAGCGACCGCGTGCAATTGCGCGTTTCCGGGCGTTTCGATTCGGTG
GAGGAGATCCGCGACTTCCCCATCCGCGTCGGCGACCGCACCTTCCGCATCGGCGACGTGGCCGAGGTTCGC
CGCGGCTTCAACGATCCGCCGGCGCCGCGCATGCGCTTCATGGGCGAGGACGCCATCGGCCTGGCGGTAGCG
ATGAAGCCGGGCGGCGACATCCTGGTGCTGGGTAAGGCCCTGGAAACCGAGTTCGCCCGCCTGCAGCAGTCG
CTGCCGGCCGGACTGGAACTGCGCAAGGTGTCCGACCAGCCGGCGGCGGTACGTACTGGGGTCGGCGAGTTC
ATCCGGGTGCTGGCCGAGGCGCTGGTGATCGTCCTGCTGGTGAGCTTCTTCTCCCTCGGCCTGCGCACAGGC
CTGGTGGTGGCGCTGTCGATCCCGCTGGTGCTGGCGATGACCTTCGCCGCCATGCATTACTTCGGCATCGGC
CTGCACAAGATTTCCCTCGGTGCCCTGGTGCTGGCGCTGGGATTGCTGGTGGACGACGCGATCATCGCGGTG
GAGATGATGGCGGTGAAGATGGAGCAGGGCTACGACCGCCTCAAGGCGGCCAGCTTCGCCTGGACCAGCACC
GCCTTCCCGATGCTCACCGGCACCCTGATCACCGCCGCCGGCTTCCTGCCGATCGCCACCGCGCAGTCCGGC
ACCGGCGAATACACCCGCTCGCTGTTCcAGGTGGTGACCATCGCCCTGGTGGTCTCCTGGTTCGCCGCGGTG
GTCTTCGTTCCCTACCTGGGGGCCAAGCTGCTGCCGGACCTGGCCAGGCTGCACGCGCAGAAGCACGGCGGC
AGCGCCGACGGCTACGATCCCTATGCAACGGCCTTCTACCAGCGCTTCCGGCGTCTGGTGGAGTGGTGCGTG
CGCTACCGCAAGACGGTGATCGTCCTGACCCTCGCCGCCTTCGTCGGCGCGCTGCTGCTGTTCCGCCTGGTG
CCGCAGCAGTTCTTCCCGCCCTCGGCGCGCCTGGAGCTGCTGCTGGACATCAAGCTGGCCGAGGGCGCCTCG
CTGCGCTCGACCGGCGAGGAAGTCCAGCGCCTGGAAAAAATGCTGCAGGGCCATGACGGCATCGACAACTAC
GTGGCCTACGTCGGCACCGGCTCGCCCCGCTTCTACCTGCCGCTGGACCAGCAGTTGCCGGCGGCCAGCTTC
GCCCAGGTGGTGGTGCTGGCCAAGGACCTGGAGAGCCGCGAGGCGCTGCGCAAGTGGCTGATCGAGCGGATG
AACGAGGACTTCCCGCACCTGCGCAGCCGCATCAGTCGCCTGGAGAACGGGCCGCCGGTGGGCTATCCGGTG
CAGTTCCGGGTTTCCGGCGAGGACATCCCGCAGGTTCGCGAACTGGCGCGCAAGGTCGCCGACAGGATGCGC
GAGAACCCGCACGTGGTGAATGTGCACCTGGATTGGGAAGAGCCGAGCAAGGTGGTGTACCTGAGCATCGAC
CAGGAGCGCGCCCGTGCCCTGGGGGTGAGCACCGCCAGCCTGTCGCAGTTCCTGCAGAGCGCGCTGACCGGT
TCGCATGTGAGCTTCTTCCGCGAAGACAACGAGCTGATCGAGATCCTCCTGCGCGGCACCGAGCAGGAGCGT
CGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATC
GCCACGCTCGAATACGGTTTCGAAGAGGGGATCatttGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGC
GCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGC
GCCGAGCTGCCGGACGGCTACCTGCTGGAGGTGGGCGGAACGGTGGAGGACGCGGCGAAGGGCCAGAGTTCG
GTGAACGCTGGCGTGCCGCTGTTCATCGTGGTGGTGTTGAGCCTGCTGATGGTGCAGTTGCGCAGCTTCTCG
CGGATGGCGATGGTATTCCTCACCGCACCGCTGGGCCTGATCGGCGTGACCCTGTTCCTGCTGCTGTTCCGC
CAGCCGTTCGGCTTCGTGGCGATGCTCGGGACCATCGCCCTGGCCGGCATGATCATGCGCAACTCGGTGATC
CTGGTGGACCAGATCGAACAGGACATCAGCCATGGACTGGATCGCTGGCACGCCATCATCGAGGCTACCGTG
CGGCGTTTCCGGCCCATCGTGCTGACCGCGCTGGCGGCGGTGCTGGCGATGATCCCGCTGTCGCGCAGCGTA
TTCTTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTG
CCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGA
Translation:
MSFNLSAWALQNRQIVLYLMILLGAVGALSYSKLGQSEDPPFTFKAMVVQTNWSGASAEEVARQVTERIEKK
LMETGDYDRIVSFSRPGVSQVTFMAREDIHSSEIPELWYQIRKKISDIRATLPQSIQGPFFNDEFGTTYGNI
YALTGKGFDYAVMKDYADRLQLQLQRIRNVGKVELIGLQDEKIWIDLSNTKLATLGLPLAAVQKALEEQNAV
ASSGFFETASDRVQLRVSGRFDSVEEIRDFPIRVGDRTFRIGDVAEVRRGFNDPPAPRMRFMGEDAIGLAVA
MKPGGDILVLGKALETEFARLQQSLPAGLELRKVSDQPAAVRTGVGEFIRVLAEALVIVLLVSFFSLGLRTG
LVVALSIPLVLAMTFAAMHYFGIGLHKISLGALVLALGLLVDDAIIAVEMMAVKMEQGYDRLKAASFAWTST
AFPMLTGTLITAAGFLPIATAQSGTGEYTRSLFQVVTIALVVSWFAAVVFVPYLGAKLLPDLARLHAQKHGG
SADGYDPYATAFYQRFRRLVEWCVRYRKTVIVLTLAAFVGALLLFRLVPQQFFPPSARLELLLDIKLAEGAS
LRSTGEEVQRLEKMLQGHDGIDNYVAYVGTGSPRFYLPLDQQLPAASFAQVVVLAKDLESREALRKWLIERM
NEDFPHLRSRISRLENGPPVGYPVQFRVSGEDIPQVRELARKVADRMRENPHVVNVHLDWEEPSKVVYLSID
QERARALGVSTASLSQFLQSALTGSHVSFFREDNELIEILLRGTEQERRDLSLLPSLAVPTENGRSVALSQI
ATLEYGFEEGIIWHRNRLPTVTVRADIYDDSLPATLVAQIAPTLEPIRAELPDGYLLEVGGTVEDAAKGQSS
VNAGVPLFIVVVLSLLMVQLRSFSRMAMVFLTAPLGLIGVTLFLLLFRQPFGFVAMLGTIALAGMIMRNSVI
LVDQIEQDISHGLDRWHAIIEATVRRFRPIVLTALAAVLAMIPLSRSVFFGPMAVAIMGGLIVATVLTLLFL
PALYAAWFRVKKDEARA*
AnnotationID:77GeneID:67
AnnotatorUID: diggins
Modification Date/Time: 2005-04-21 10:10:06
Gene Name:
Confidence Code:3
GeneProduct:putative efflux transmembrane protein
Cell Localization:()
Synonyms:PA3676
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0841,pfam00873,COG3696
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67138.1|AE016342_10 (AE016779) RND efflux transporter [Pseudomonas putida KT2440]
          Length = 1021

 Score = 1666 bits (4315), Expect = 0.0
 Identities = 841/1020 (82%), Positives = 936/1020 (91%)

>dbj|BAC47688.1| (AP005943) cation efflux system protein [Bradyrhizobium japonicum
            USDA 110]
          Length = 1045

 Score =  963 bits (2489), Expect = 0.0
 Identities = 501/1022 (49%), Positives = 690/1022 (67%), Gaps = 7/1022 (0%)

>gb|AAO58617.1| (AE016874) AcrB/AcrD/AcrF family protein [Pseudomonas syringae pv.
            tomato str. DC3000]
          Length = 1033

 Score =  912 bits (2356), Expect = 0.0
 Identities = 469/1025 (45%), Positives = 665/1025 (64%), Gaps = 13/1025 (1%)









 
Structural Features:
COG0841, AcrB, Cation/multidrug efflux pump [Defense mechanisms]
CD-Length = 1009 residues, 100.0% aligned
Score =  818 bits (2114), Expect = 0.0
Identities = 313/1031 (30%), Positives = 494/1031 (47%), Gaps = 38/1031 (3%)

pfam00873, ACR_tran, AcrB/AcrD/AcrF family
CD-Length = 1017 residues,  99.6% aligned
Score =  487 bits (1255), Expect = 6e-138
Identities = 294/1033 (28%), Positives = 487/1033 (47%), Gaps = 45/1033 (4%)

COG3696, Putative silver efflux pump [Inorganic ion transport and metabolism]
CD-Length = 1027 residues,  98.9% aligned
Score =  393 bits (1011), Expect = 1e-109
Identities = 266/1045 (25%), Positives = 472/1045 (45%), Gaps = 61/1045 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:137




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17279




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31869




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:67_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:67
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
138
                           PA3676_tr
                           translation of PA3676
                           1025
                           99.80
                           99.90
                           0
                           5115.0
     

     
     
13324
                           gb|AAG07064.1|AE004787_4
                           (AE004787) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
                           1025
                           99.80
                           99.90
                           0
                           5115.0
     

     
     
13325
                           gb|AAN67138.1|AE016342_10
                           (AE016779) RND efflux transporter [Pseudomonas putida KT2440]
                           1025
                           82.04
                           91.31
                           4
                           4314.0
     

     
     
13326
                           gb|AAQ61585.1|
                           (AE016923) probable transmembrane drug efflux protein [Chromobacterium violaceum ATCC 12472]
                           1037
                           55.35
                           73.48
                           20
                           2888.0
     

     
     
13327
                           emb|CAD15356.1|
                           (AL646065) PROBABLE TRANSMEMBRANE DRUG EFFLUX PROTEIN [Ralstonia solanacearum]
                           1045
                           51.19
                           69.76
                           26
                           2716.0
     

     






      

          
GC ORFID: 43069How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3676Glimmer Score: 

          
Start: 3515184Stop: 3512107

          
Length: 3078


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3676 translation of PA3676Homolog Bit Score 1974.0

          
Other Homologs:  t_PA0158 (ORF: BLASTX 897.0), psyr_15may02_Scaffold11_revised_gene1933 (ORF: BLASTX 853.0), psyr_15may02_Scaffold11_revised_gene1820 (ORF: BLASTX 115.0), t_PA3676 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCTTCAACCTTTCCGCCTGGGCGTTGCAGAATCGCCAGATCGTCCTGTACCTGATGATCCTGCTTGGC 
GCGGTTGGCGCGCTGTCCTACAGCAAGCTGGGGCAGAGCGAAGACCCGCCGTTCACCTTCAAGGCCATGGTG 
GTGCAGACCAACTGGTCGGGTGCCAGCGCCGAAGAGGTGGCCCGGCAGGTCACCGAGCGTATCGAGAAGAAG 
CTGATGGAAACCGGCGACTACGATCGCATCGTGTCCTTCTCCCGCCCCGGCGTCTCGCAGGTGACCTTCATG 
GCCCGCGAGGACATCCATTCCAGCGAGATCCCCGAACTCTGGTACCAGATCCGCAAGAAGATCAGCGACATT 
CGCGCCACCTTGCCGCAAAGCATCCAGGGCCCGTTCTTCAACGACGAGTTCGGCACCACCTACGGCAACATC 
TATGCGCTCACCGGCAAGGGCTTCGACTATGCGGTGATGAAGGACTATGCCGACCGCCTGCAACTGCAATTG 
CAGCGGATCAGGAACGTCGGCAAGGTCGAACTGATCGGCCTGCAGGACGAGAAGATCTGGATCGACCTGTCC 
AACACCAAGCTGGCCACCCTCGGCCTGCCCCTGGCGGCGGTGCAGAAGGCGCTGGAGGAACAGAACGCGGTG 
GCCTCCTCCGGGTTCTTCGAGACCGCCAGCGACCGCGTGCAATTGCGCGTTTCCGGGCGTTTCGATTCGGTG 
GAGGAGATCCGCGACTTCCCCATCCGCGTCGGCGACCGCACCTTCCGCATCGGCGACGTGGCCGAGGTTCGC 
CGCGGCTTCAACGATCCGCCGGCGCCGCGCATGCGCTTCATGGGCGAGGACGCCATCGGCCTGGCGGTAGCG 
ATGAAGCCGGGCGGCGACATCCTGGTGCTGGGTAAGGCCCTGGAAACCGAGTTCGCCCGCCTGCAGCAGTCG 
CTGCCGGCCGGACTGGAACTGCGCAAGGTGTCCGACCAGCCGGCGGCGGTACGTACTGGGGTCGGCGAGTTC 
ATCCGGGTGCTGGCCGAGGCGCTGGTGATCGTCCTGCTGGTGAGCTTCTTCTCCCTCGGCCTGCGCACAGGC 
CTGGTGGTGGCGCTGTCGATCCCGCTGGTGCTGGCGATGACCTTCGCCGCCATGCATTACTTCGGCATCGGC 
CTGCACAAGATTTCCCTCGGTGCCCTGGTGCTGGCGCTGGGATTGCTGGTGGACGACGCGATCATCGCGGTG 
GAGATGATGGCGGTGAAGATGGAGCAGGGCTACGACCGCCTCAAGGCGGCCAGCTTCGCCTGGACCAGCACC 
GCCTTCCCGATGCTCACCGGCACCCTGATCACCGCCGCCGGCTTCCTGCCGATCGCCACCGCGCAGTCCGGC 
ACCGGCGAATACACCCGCTCGCTGTTCcAGGTGGTGACCATCGCCCTGGTGGTCTCCTGGTTCGCCGCGGTG 
GTCTTCGTTCCCTACCTGGGGGCCAAGCTGCTGCCGGACCTGGCCAGGCTGCACGCGCAGAAGCACGGCGGC 
AGCGCCGACGGCTACGATCCCTATGCAACGGCCTTCTACCAGCGCTTCCGGCGTCTGGTGGAGTGGTGCGTG 
CGCTACCGCAAGACGGTGATCGTCCTGACCCTCGCCGCCTTCGTCGGCGCGCTGCTGCTGTTCCGCCTGGTG 
CCGCAGCAGTTCTTCCCGCCCTCGGCGCGCCTGGAGCTGCTGCTGGACATCAAGCTGGCCGAGGGCGCCTCG 
CTGCGCTCGACCGGCGAGGAAGTCCAGCGCCTGGAAAAAATGCTGCAGGGCCATGACGGCATCGACAACTAC 
GTGGCCTACGTCGGCACCGGCTCGCCCCGCTTCTACCTGCCGCTGGACCAGCAGTTGCCGGCGGCCAGCTTC 
GCCCAGGTGGTGGTGCTGGCCAAGGACCTGGAGAGCCGCGAGGCGCTGCGCAAGTGGCTGATCGAGCGGATG 
AACGAGGACTTCCCGCACCTGCGCAGCCGCATCAGTCGCCTGGAGAACGGGCCGCCGGTGGGCTATCCGGTG 
CAGTTCCGGGTTTCCGGCGAGGACATCCCGCAGGTTCGCGAACTGGCGCGCAAGGTCGCCGACAGGATGCGC 
GAGAACCCGCACGTGGTGAATGTGCACCTGGATTGGGAAGAGCCGAGCAAGGTGGTGTACCTGAGCATCGAC 
CAGGAGCGCGCCCGTGCCCTGGGGGTGAGCACCGCCAGCCTGTCGCAGTTCCTGCAGAGCGCGCTGACCGGT 
TCGCATGTGAGCTTCTTCCGCGAAGACAACGAGCTGATCGAGATCCTCCTGCGCGGCACCGAGCAGGAGCGT 
CGCGACCTGTCGTTGCTGCCGAGCCTGGCGGTGCCGACCGAGAATGGCCGGAGCGTGGCGCTGTCGCAGATC 
GCCACGCTCGAATACGGTTTCGAAGAGGGGATCatttGGCACCGCAACCGCCTGCCGACGGTCACCGTGCGC 
GCCGATATCTACGACGATTCGCTGCCGGCGACCCTGGTCGCGCAGATCGCCCCGACCCTGGAACCGATCCGC 
GCCGAGCTGCCGGACGGCTACCTGCTGGAGGTGGGCGGAACGGTGGAGGACGCGGCGAAGGGCCAGAGTTCG 
GTGAACGCTGGCGTGCCGCTGTTCATCGTGGTGGTGTTGAGCCTGCTGATGGTGCAGTTGCGCAGCTTCTCG 
CGGATGGCGATGGTATTCCTCACCGCACCGCTGGGCCTGATCGGCGTGACCCTGTTCCTGCTGCTGTTCCGC 
CAGCCGTTCGGCTTCGTGGCGATGCTCGGGACCATCGCCCTGGCCGGCATGATCATGCGCAACTCGGTGATC 
CTGGTGGACCAGATCGAACAGGACATCAGCCATGGACTGGATCGCTGGCACGCCATCATCGAGGCTACCGTG 
CGGCGTTTCCGGCCCATCGTGCTGACCGCGCTGGCGGCGGTGCTGGCGATGATCCCGCTGTCGCGCAGCGTA 
TTCTTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTG 
CCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGA