ViewGene
Locus Name:
GeneID:
Location:

GeneID:663
TrivialName: PA14_64770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64770
PAO1 Ortholog Locus:PA4901
Sequence Length:1587
Protein Length:528
Start: 5771732
Stop: 5770146
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08286.1|AE004903_4 (AE004903) benzoylformate decarboxylase [Pseudomonas aeruginosa PAO1]
Identities = 526/528 (99%), Positives = 527/528 (99%)
Sequence:
ATGAAAACCGTCCATTCCGCGTCTTACGAGATCCTCCGTCGCCACGGCCTGACCACCGTCTTCGGCAACCCC
GGCTCCAACGAGCTGCCGTTCCTCAAGGACTTCCCCGAGGATTTCCGCTACATCCTCGGCCTGCACGAGGGC
GCGGTGGTCGGCATGGCCGATGGCTTCGCCCTGGCCAGCGGCCGCCCGGCCTTCGTCAACCTGCATGCGGCG
GCGGGCACCGGCAACGGCATGGGCGCGCTGACCAACGCCTGGTACTCCCATAGCCCCCTGGTCATCACCGCC
GGGCAGCAGGTGCGCTCGATGATCGGGGTCGAGGCGATGCTCGCCAATGTCGATGCCGGACAACTGCCCAAG
CCGCTGGTGAAATGGAGCCACGAGCCGGCCTGCGCCCAGGATGTGCCGCGCGCGTTGAGCCAGGCGATCCAG
ACCGCCAGCCTGCCGCCGCGGGCGCCGGTGTACCTGTCGATTCCCTACGACGACTGGGCGCAACCGGCGCCG
GCCGGCGTCGAACACCTGGCCGCGCGCCAGGTCAGCGGCGCCGCGCTGCCGGCGCCGGCATTGCTGGCCGAA
CTCGGCGAGCGCCTGTCGCGCTCGCGCAACCCGGTGCTGGTGCTCGGTCCGGACGTCGACGGGGCGAATGCC
AACGGCCTGGCCGTGCAACTGGCGGAGAAGCTGCGGATGCCGGCCTGGGTCGCGCCGTCGGCCTCGCGTTGT
CCGTTCCCCACCCGCCATGCCTGCTTCCGCGGCGTGCTGCCGGCGGCCATCGCCGGGATCAGCCGGCTGCTG
GACGGCCACGACCTGATCCTGGTGGTCGGCGCGCCGGTGTTCCGCTATCACCAGTTCGCCCCCGGCGATTAC
CTGCCGGCCGGCGCCGAGCTGGTGCAGGTCACCTGCGATCCCGGCGAAGCGGCACGGGCGCCGATGGGCGAC
GCGTTGGTCGGCGACATCGCGCTGACCCTCGAGGCACTGCTCGAACAGGTCCGCCCCAGTGCGCGGCCGTTG
CCCGAGGCGCTGCCGCGACCGCCGGCGCTGGCCGAGGAGGGCGGACCGCTGCGGCCGGAAACCGTGTTCGAC
GTGATCGATGCGCTGGCGCCGCGCGACGCCATCTTCGTCAAGGAGTCGACTTCCACCGTGACCGCCTTCTGG
CAGCGCGTGGAGATGCGCGAGCCGGGCAGCTACTTCTTCCCGGCCGCCGGCGGACTCGGCTTCGGCCTGCCG
GCGGCGGTCGGCGCGCAACTGGCGCAGCCGCGGCGGCAGGTGATCGGGATCATCGGCGACGGCTCGGCCAAC
TATGGCATCACCGCACTCTGGAGCGCCGCGCAATACCGTGTGCCGGCGGTGTTCATCATCCTCAAGAACGGT
ACCTACGGCGCCCTGCGCTGGTTCGCCGGGGTGCTCGAGGTGCCCGATGCGCCGGGGCTCGACGTGCCCGGC
CTGGACTTCTGCGCGATCGCCCGCGGCTATGGCGTCGAGGCGCTCCACGCGGCCACCCGCGAAGAGCTGGAA
AGCGCCTTGAAGCATGCCCTGGCCGCTGATCGCCCGGTTTTGATCGAGGTGCCGACGCAAACCATCGAACCC
TGA
Translation:
MKTVHSASYEILRRHGLTTVFGNPGSNELPFLKDFPEDFRYILGLHEGAVVGMADGFALASGRPAFVNLHAA
AGTGNGMGALTNAWYSHSPLVITAGQQVRSMIGVEAMLANVDAGQLPKPLVKWSHEPACAQDVPRALSQAIQ
TASLPPRAPVYLSIPYDDWAQPAPAGVEHLAARQVSGAALPAPALLAELGERLSRSRNPVLVLGPDVDGANA
NGLAVQLAEKLRMPAWVAPSASRCPFPTRHACFRGVLPAAIAGISRLLDGHDLILVVGAPVFRYHQFAPGDY
LPAGAELVQVTCDPGEAARAPMGDALVGDIALTLEALLEQVRPSARPLPEALPRPPALAEEGGPLRPETVFD
VIDALAPRDAIFVKESTSTVTAFWQRVEMREPGSYFFPAAGGLGFGLPAAVGAQLAQPRRQVIGIIGDGSAN
YGITALWSAAQYRVPAVFIILKNGTYGALRWFAGVLEVPDAPGLDVPGLDFCAIARGYGVEALHAATREELE
SALKHALAADRPVLIEVPTQTIEP*
AnnotationID:672GeneID:663
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 07:07:58
Gene Name:mdlC
Confidence Code:2
GeneProduct:benzoylformate decarboxylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0028
Secondary Functional Category(ies):
EC Number:4.1.1.7
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC15502.1| (AY143338) benzoylformate decarboxylase [Pseudomonas putida]
          Length = 528

 Score =  691 bits (1782), Expect = 0.0
 Identities = 331/523 (63%), Positives = 402/523 (76%)

>emb|CAB76356.1| (AL939131) putative decarboxylase. [Streptomyces coelicolor A3(2)]
          Length = 526

 Score =  586 bits (1511), Expect = e-166
 Identities = 304/528 (57%), Positives = 363/528 (68%), Gaps = 3/528 (0%)








 
Structural Features:
COG0028, IlvB, Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, 
pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, 
phosphonopyruvate decarboxylase] 
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 550 residues,  97.3% aligned
Score =  361 bits (929), Expect = 8e-101
Identities = 169/539 (31%), Positives = 246/539 (45%), Gaps = 15/539 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1329




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18471




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35158




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:663_cdd.html


    		




 
URL:

















    ReferenceID:35935




  
Author/Investigator(s):
        Tsou AY, Ransom SC, Gerlt JA, Buechter DD, Babbitt PC, Kenyon GL.





  
Title:
        Mandelate pathway of Pseudomonas putida: sequence relationships involving mandelate racemase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase and expression of benzoylformate decarboxylase in Escherichia coli.




  
PubMed:
        2271624


MedLine:
        




    
Source:
Biochemistry. 1990 Oct 23;29(42):9856-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2271624









           
Homologs By Global Alignment 

           

       



     
Gene ID:663
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1118
                           PA4901_tr
                           translation of PA4901
                           528
                           99.62
                           99.81
                           0
                           2727.0
     

     
     
41897
                           gb|AAG08286.1|AE004903_4
                           (AE004903) benzoylformate decarboxylase [Pseudomonas aeruginosa PAO1]
                           528
                           99.62
                           99.81
                           0
                           2727.0
     

     
     
41898
                           gb|AAC15502.1|
                           (AY143338) benzoylformate decarboxylase [Pseudomonas putida]
                           530
                           62.64
                           76.22
                           4
                           1782.0
     

     
     
41899
                           emb|CAB76356.1|
                           (AL939131) putative decarboxylase. [Streptomyces coelicolor A3(2)]
                           529
                           57.46
                           68.62
                           4
                           1515.0
     

     
     
41900
                           dbj|BAC51681.1|
                           (AP005958) benzoylformate decarboxylase [Bradyrhizobium japonicum USDA 110]
                           548
                           44.34
                           57.29
                           26
                           1120.0
     

     






      

          
GC ORFID: 43665How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4901Glimmer Score: 

          
Start: 5035413Stop: 5033827

          
Length: 1587


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4901 translation of PA4901Homolog Bit Score 1055.0

          
Other Homologs:  t_PA4901 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAACCGTCCATTCCGCGTCTTACGAGATCCTCCGTCGCCACGGCCTGACCACCGTCTTCGGCAACCCC 
GGCTCCAACGAGCTGCCGTTCCTCAAGGACTTCCCCGAGGATTTCCGCTACATCCTCGGCCTGCACGAGGGC 
GCGGTGGTCGGCATGGCCGATGGCTTCGCCCTGGCCAGCGGCCGCCCGGCCTTCGTCAACCTGCATGCGGCG 
GCGGGCACCGGCAACGGCATGGGCGCGCTGACCAACGCCTGGTACTCCCATAGCCCCCTGGTCATCACCGCC 
GGGCAGCAGGTGCGCTCGATGATCGGGGTCGAGGCGATGCTCGCCAATGTCGATGCCGGACAACTGCCCAAG 
CCGCTGGTGAAATGGAGCCACGAGCCGGCCTGCGCCCAGGATGTGCCGCGCGCGTTGAGCCAGGCGATCCAG 
ACCGCCAGCCTGCCGCCGCGGGCGCCGGTGTACCTGTCGATTCCCTACGACGACTGGGCGCAACCGGCGCCG 
GCCGGCGTCGAACACCTGGCCGCGCGCCAGGTCAGCGGCGCCGCGCTGCCGGCGCCGGCATTGCTGGCCGAA 
CTCGGCGAGCGCCTGTCGCGCTCGCGCAACCCGGTGCTGGTGCTCGGTCCGGACGTCGACGGGGCGAATGCC 
AACGGCCTGGCCGTGCAACTGGCGGAGAAGCTGCGGATGCCGGCCTGGGTCGCGCCGTCGGCCTCGCGTTGT 
CCGTTCCCCACCCGCCATGCCTGCTTCCGCGGCGTGCTGCCGGCGGCCATCGCCGGGATCAGCCGGCTGCTG 
GACGGCCACGACCTGATCCTGGTGGTCGGCGCGCCGGTGTTCCGCTATCACCAGTTCGCCCCCGGCGATTAC 
CTGCCGGCCGGCGCCGAGCTGGTGCAGGTCACCTGCGATCCCGGCGAAGCGGCACGGGCGCCGATGGGCGAC 
GCGTTGGTCGGCGACATCGCGCTGACCCTCGAGGCACTGCTCGAACAGGTCCGCCCCAGTGCGCGGCCGTTG 
CCCGAGGCGCTGCCGCGACCGCCGGCGCTGGCCGAGGAGGGCGGACCGCTGCGGCCGGAAACCGTGTTCGAC 
GTGATCGATGCGCTGGCGCCGCGCGACGCCATCTTCGTCAAGGAGTCGACTTCCACCGTGACCGCCTTCTGG 
CAGCGCGTGGAGATGCGCGAGCCGGGCAGCTACTTCTTCCCGGCCGCCGGCGGACTCGGCTTCGGCCTGCCG 
GCGGCGGTCGGCGCGCAACTGGCGCAGCCGCGGCGGCAGGTGATCGGGATCATCGGCGACGGCTCGGCCAAC 
TATGGCATCACCGCACTCTGGAGCGCCGCGCAATACCGTGTGCCGGCGGTGTTCATCATCCTCAAGAACGGT 
ACCTACGGCGCCCTGCGCTGGTTCGCCGGGGTGCTCGAGGTGCCCGATGCGCCGGGGCTCGACGTGCCCGGC 
CTGGACTTCTGCGCGATCGCCCGCGGCTATGGCGTCGAGGCGCTCCACGCGGCCACCCGCGAAGAGCTGGAA 
AGCGCCTTGAAGCATGCCCTGGCCGCTGATCGCCCGGTTTTGATCGAGGTGCCGACGCAAACCATCGAACCC 
TGA