ViewGene
Locus Name:
GeneID:
Location:

GeneID:661
TrivialName: PA14_46880
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_46880
PAO1 Ortholog Locus:PA1345
Sequence Length:1590
Protein Length:529
Start: 4175940
Stop: 4174351
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04734.1|AE004564_3 (AE004564) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 526/529 (99%), Positives = 527/529 (99%)
Sequence:
ATGTCAGCAGCCTCGTTAGCCACCCTCGACGGGTCGCTCCGCATGACCCCCGTCGAGCAACCCCGACCGCCG
GCTCTCGCCGACCTCGGCAGCCGCCTGATGATCGTCGTCGAGCGACGCGAGGACTGGGCCTCCTACCTGCCC
AGCGAGGAAGTACTCACCGCCCAGGAATACCTCGAGCAATCGGCGCGCGACCACGGCGGCCGCCGCGTGCAG
GTGATCAACCTGTGCCGCGGCTACAAGTACCTCGGCCTCGGCTACTACTGTTCGCTGCTGGCCGAGGCCCGC
GGCCACCGGGTGATCCCGTCGCTGCGCTGCATCAGCGAACTGGCTCGCAAGTCGGTCTACGGGCTGGCCCTG
GGCGGCCTCGACAAGGCCCTGGACAAGGCGCTGTGCCGTACGCCCTATGGCGCCACCGACGGCTTCACCCTG
ACCCTGTATTTCGGCCATACCGAGTTCGAGCCACTGGCCGACCTCGCTCGCCAGTTGTTCGAAGCCTTTCCC
TGCCCGATCCTGTTGGTGGAGTTCCGTCGCCAGCAGGGCTGGCATATCGAGGGCGTGCGCCCTGGCGTGCTG
AACCGCTTGCGCAGCGACCAGGAGGATGTCTTCGCCGATGCCCTCGACGGTTTCAGCCGGCGCGCCTGGCGC
ATGCCGCGTTCGCGGCGGGTGGCACGCTACGACATGGCGATCCTGCACGATCCCGACGAAGCGCTGCCGCCG
TCCAACCCGCAGGCGCTGGCCAACTTCGTACGGGTCGGCGCCAGCCTCGGCATCGACGTCGAACTGATCGGA
CGCAAGGACTACGCGCGCCTGGCCGAGTTCGACGCCCTGCTGATCCGCGAGACCACCCGGGTCGACCACCAT
ACCTATCGCTTCGCCGAGAAGGCCGAGCGCGAAGGCCTGGTGGTGATGGACGACCCGGCATCGATCCTGCGC
TGCACCAACAAGGTCTACCTGGCCGACCTGCTCGGTTGCCGGCAACTGGGCATGCCGCTCACCGAGATCCTC
TACAAGGAACGCCCGCAGGACTGGCAGCGGGTGGCCCGGCGCCTGGGCTTCCCGCTGGTGCTGAAGATTCCC
GATGGCTGCTTCTCCCGTGGCGTGGTCAAGGTCAACGACGACAGCGAACTGCTGGCGGCGGCGAGCGAGCTG
TTCGAGCGCTCGGTCCTGTTGCTGGCCCAGGAGTTCTTCTACACCGAGTACGACTGGCGCATCGGCGTGCTC
GACCGCCAACCGCTGTACGCCTGCCAGTACTTCATGTCGCGCGGCCACTGGCAGATCTACAACCACAAGGCC
GACGGCCAGGACGTCAACGGCGAGTGCCGCGCCGTGCCGCTGGAGCAGGTGCCGCCGGCGGTGCTGGAGCTG
GCCCTGGAGGCCGCCGGGCGGATCGGCGACGGCCTCTACGGGGTGGACCTGAAGCAGGCCGGCGACAAGGTG
CTGGTCATCGAGGTCAACGACAACCCGAATATCGATGCCGGCATCGAGGACGGCCAGCTCGGCGACGAACTC
TACCGGCGCATCCTCCAGGCCTTCGTCCAGCGCCTGGAACTCAAGCACCGTGGCCAGTTGAGCAAGGCGCTG
CGCTGA
Translation:
MSAASLATLDGSLRMTPVEQPRPPALADLGSRLMIVVERREDWASYLPSEEVLTAQEYLEQSARDHGGRRVQ
VINLCRGYKYLGLGYYCSLLAEARGHRVIPSLRCISELARKSVYGLALGGLDKALDKALCRTPYGATDGFTL
TLYFGHTEFEPLADLARQLFEAFPCPILLVEFRRQQGWHIEGVRPGVLNRLRSDQEDVFADALDGFSRRAWR
MPRSRRVARYDMAILHDPDEALPPSNPQALANFVRVGASLGIDVELIGRKDYARLAEFDALLIRETTRVDHH
TYRFAEKAEREGLVVMDDPASILRCTNKVYLADLLGCRQLGMPLTEILYKERPQDWQRVARRLGFPLVLKIP
DGCFSRGVVKVNDDSELLAAASELFERSVLLLAQEFFYTEYDWRIGVLDRQPLYACQYFMSRGHWQIYNHKA
DGQDVNGECRAVPLEQVPPAVLELALEAAGRIGDGLYGVDLKQAGDKVLVIEVNDNPNIDAGIEDGQLGDEL
YRRILQAFVQRLELKHRGQLSKALR*
AnnotationID:670GeneID:661
AnnotatorUID: danlee
Modification Date/Time: 2006-02-03 17:05:39
Gene Name:
Confidence Code:3
GeneProduct:possible glutathione synthase
Cell Localization:()
Synonyms:PA1345
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:ribosomal protein S6 modification enzyme
Functional Category Confidence Code:5
COGs:COG0189
Secondary Functional Category(ies):Translation, ribosomal structure and biogenesis
EC Number:6.3.2.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68564.1|AE016488_8 (AE016785) ribosomal protein S6 modification protein-related
           protein [Pseudomonas putida KT2440]
          Length = 526

 Score =  718 bits (1854), Expect = 0.0
 Identities = 347/501 (69%), Positives = 411/501 (82%), Gaps = 2/501 (0%)

>gb|AAO10191.1|AE016802_234 (AE016802) Glutathione synthase; Ribosomal protein S6 modification
           enzyme [Vibrio vulnificus CMCP6]
          Length = 483

 Score =  437 bits (1125), Expect = e-122
 Identities = 225/493 (45%), Positives = 320/493 (64%), Gaps = 17/493 (3%)

>gb|AAO76581.1| (AE016932) ribosomal protein S6 modification protein-related
           protein [Bacteroides thetaiotaomicron VPI-5482]
          Length = 488

 Score =  404 bits (1037), Expect = e-111
 Identities = 206/490 (42%), Positives = 306/490 (62%), Gaps = 4/490 (0%)








 
Structural Features:
COG0189, RimK, Glutathione synthase/Ribosomal protein S6 modification enzyme 
(glutaminyl transferase) 
[Coenzyme metabolism / Translation, ribosomal structure and biogenesis]
CD-Length = 318 residues, 100.0% aligned
Score =  260 bits (665), Expect = 3e-70
Identities = 96/326 (29%), Positives = 146/326 (44%), Gaps = 12/326 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1325




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18467




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37448




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:661_cdd.html


    		




 
URL:

















    ReferenceID:38174




  
Author/Investigator(s):
        Okumura N, Masamoto K, Wada H.





  
Title:
        The gshB gene in the cyanobacterium Synechococcus sp. PCC 7942 encodes a functional glutathione synthetase.




  
PubMed:
        9308172


MedLine:
        




    
Source:
Microbiology. 1997 Sep;143 ( Pt 9):2883-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9308172&query_hl=26

















    ReferenceID:38175




  
Author/Investigator(s):
        Kang WK, Icho T, Isono S, Kitakawa M, Isono K.





  
Title:
        Characterization of the gene rimK responsible for the addition of glutamic acid residues to the C-terminus of ribosomal protein S6 in Escherichia coli K12.




  
PubMed:
        2570347


MedLine:
        




    
Source:
Mol Gen Genet. 1989 Jun;217(2-3):281-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2570347&query_hl=29









           
Homologs By Global Alignment 

           

       



     
Gene ID:661
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1115
                           PA1345_tr
                           translation of PA1345
                           529
                           99.43
                           99.62
                           0
                           2732.0
     

     
     
41887
                           gb|AAG04734.1|AE004564_3
                           (AE004564) hypothetical protein PA1345 [Pseudomonas aeruginosa PAO1]
                           529
                           99.43
                           99.62
                           0
                           2732.0
     

     
     
1116
                           PA1944_tr
                           translation of PA1944
                           529
                           66.54
                           78.82
                           34
                           1888.0
     

     
     
41888
                           gb|AAG05332.1|AE004621_3
                           (AE004621) hypothetical protein PA1944 [Pseudomonas aeruginosa PAO1]
                           529
                           66.54
                           78.82
                           34
                           1888.0
     

     
     
41889
                           gb|AAN68564.1|AE016488_8
                           (AE016785) ribosomal protein S6 modification protein-related protein [Pseudomonas putida KT2440]
                           532
                           66.16
                           79.32
                           9
                           1852.0
     

     






      

          
GC ORFID: 43663How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1345Glimmer Score: 

          
Start: 777969Stop: 779558

          
Length: 1590


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1345 translation of PA1345Homolog Bit Score 1056.0

          
Other Homologs:  t_PA1944 (ORF: BLASTX 734.0), t_PA1345 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCAGCAGCCTCGTTAGCCACCCTCGACGGGTCGCTCCGCATGACCCCCGTCGAGCAACCCCGACCGCCG 
GCTCTCGCCGACCTCGGCAGCCGCCTGATGATCGTCGTCGAGCGACGCGAGGACTGGGCCTCCTACCTGCCC 
AGCGAGGAAGTACTCACCGCCCAGGAATACCTCGAGCAATCGGCGCGCGACCACGGCGGCCGCCGCGTGCAG 
GTGATCAACCTGTGCCGCGGCTACAAGTACCTCGGCCTCGGCTACTACTGTTCGCTGCTGGCCGAGGCCCGC 
GGCCACCGGGTGATCCCGTCGCTGCGCTGCATCAGCGAACTGGCTCGCAAGTCGGTCTACGGGCTGGCCCTG 
GGCGGCCTCGACAAGGCCCTGGACAAGGCGCTGTGCCGTACGCCCTATGGCGCCACCGACGGCTTCACCCTG 
ACCCTGTATTTCGGCCATACCGAGTTCGAGCCACTGGCCGACCTCGCTCGCCAGTTGTTCGAAGCCTTTCCC 
TGCCCGATCCTGTTGGTGGAGTTCCGTCGCCAGCAGGGCTGGCATATCGAGGGCGTGCGCCCTGGCGTGCTG 
AACCGCTTGCGCAGCGACCAGGAGGATGTCTTCGCCGATGCCCTCGACGGTTTCAGCCGGCGCGCCTGGCGC 
ATGCCGCGTTCGCGGCGGGTGGCACGCTACGACATGGCGATCCTGCACGATCCCGACGAAGCGCTGCCGCCG 
TCCAACCCGCAGGCGCTGGCCAACTTCGTACGGGTCGGCGCCAGCCTCGGCATCGACGTCGAACTGATCGGA 
CGCAAGGACTACGCGCGCCTGGCCGAGTTCGACGCCCTGCTGATCCGCGAGACCACCCGGGTCGACCACCAT 
ACCTATCGCTTCGCCGAGAAGGCCGAGCGCGAAGGCCTGGTGGTGATGGACGACCCGGCATCGATCCTGCGC 
TGCACCAACAAGGTCTACCTGGCCGACCTGCTCGGTTGCCGGCAACTGGGCATGCCGCTCACCGAGATCCTC 
TACAAGGAACGCCCGCAGGACTGGCAGCGGGTGGCCCGGCGCCTGGGCTTCCCGCTGGTGCTGAAGATTCCC 
GATGGCTGCTTCTCCCGTGGCGTGGTCAAGGTCAACGACGACAGCGAACTGCTGGCGGCGGCGAGCGAGCTG 
TTCGAGCGCTCGGTCCTGTTGCTGGCCCAGGAGTTCTTCTACACCGAGTACGACTGGCGCATCGGCGTGCTC 
GACCGCCAACCGCTGTACGCCTGCCAGTACTTCATGTCGCGCGGCCACTGGCAGATCTACAACCACAAGGCC 
GACGGCCAGGACGTCAACGGCGAGTGCCGCGCCGTGCCGCTGGAGCAGGTGCCGCCGGCGGTGCTGGAGCTG 
GCCCTGGAGGCCGCCGGGCGGATCGGCGACGGCCTCTACGGGGTGGACCTGAAGCAGGCCGGCGACAAGGTG 
CTGGTCATCGAGGTCAACGACAACCCGAATATCGATGCCGGCATCGAGGACGGCCAGCTCGGCGACGAACTC 
TACCGGCGCATCCTCCAGGCCTTCGTCCAGCGCCTGGAACTCAAGCACCGTGGCCAGTTGAGCAAGGCGCTG 
CGCTGA