ViewGene
Locus Name:
GeneID:
Location:

GeneID:660
TrivialName: PA14_70110
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70110
PAO1 Ortholog Locus:PA5310
Sequence Length:1590
Protein Length:529
Start: 6252441
Stop: 6254030
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08695.1|AE004943_11 (AE004943) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 525/529 (99%), Positives = 526/529 (99%)
Sequence:
ATGAGCCGGTCGTTCGAGTTTCCACGGGAGATGTCCTTGCTTCGCCGATTCCTCCTGCTGGCCCTCCTGGCC
CTCTCCGGCTGCGCCAGCACTCCTCCGCCGCAACCGTCGTCGGCACTGCCCGCCGAGGGTACCTGGCTGGCC
AGGCAGGCCGAAATCCAGGGACGCGACCACCCCGGCCAGTCCGGCTTCCACCTCCTGGGCGCCAGCGAGGAT
GCCTTCGTCGCCCGCGCGGCGCTGATTCGCGCAGCCCAGCGCAGCCTCGACATCCAGTACTACATCGTCCAC
GACGGACTGACCACGCGCGCCCTCGCCTACGAGTTGCTCAAGGCAGCCGACCGTGGCGTGCGGGTACGCATC
CTCATCGACGACACCGCCAGCGATGGCTGGGACTACGAGATCGGTGTGCTCTCCGCCCATCCGAACATCCAG
GTGCGCCTGTTCAACCCGTTGCACCTGGGCCGCGCCACCGGCATCACGCGAGGCGTCGGCCGCCTGTTCAAC
CTGTCCCAGCAACACCGGCGCATGCACAACAAGCTGTGGCTGGCCGACGGCACGGCGGCCATCGTCGGCGGA
CGCAACCTCGGCGACGAGTATTTCAACGCCAAGCCGGAGATGAACTTCACCGACCTCGACCTGCTCGGCGTC
GGCCCCATCGCCAACCAGCTCTCGCACAGCTTCGACCAGTACTGGAACAGCGCCATCAGCCGGCCGATCGAG
GATTTCCTCTGGCGCGCGCCCTATCCCGGCGAACTGGAGTCGGCCCGACGCAAGTTGCAGCGCTACCTGCGC
AAGGAGTCGGTGAAGGAGTCCGGCTACATCCGCCACCTGTTCGACCACGGCGACCAGCCACGCCTGGGCAAC
TGGCTGGAGAATCTCACCTGGGCACGCGCCGAGGCGATCTGGGATGCGCCGCTGAAAGTATTGAGCCGCGGC
GAGCCCGACTCGCACCTATTGCTCAGTCCGCACCTGGCGGGCCTGTTCAAGGGCGTGCAGAAGGAACTGATC
CTGGTCTCGGCGTATTTCGTTCCGGCCAAGGACGGTCTCAACTACCTCACCGGCAAGGCCGACAGCGGCGTC
CGCGTGCGCCTGCTGACCAACTCCCTGGAAGCCACCGACGTGCCCGCCGTGCATGCCGGCTACGCACCCTAT
CGGATGGCGCTGCTGGAACATGGCGTGAAGCTCTACGAGCTGCGCGCCAATCCCGACCAGCCGCTGAGCGGC
GCGCCCTGGCGCCTGCACGGATCGAGCAGCGCCAGCCTGCACAGCAAGGCGATGGTCTTCGACAGGCGCAAG
GTATTCATCGGTTCGTTCAACTTCGATCCGCGCTCGATCCTGTGGAACACCGAAGTCGGGGTCATCGTCGAC
AGTCCGCTGCTGGCCGAGCAGGTCCGCCAACTGGCGCTGGAGGGCATGGCCCCGAGCGTCAGCTACCAGGTT
CGCATCGACCGCAGCGGCAGCCGGCCGAAGCTGGTCTGGATCGACGAACGCGACGGCCGGGCCCAGGTCCTG
CGCCACGAGCCGGGTAGCCTGTGGCGACGCCTGAACGCCTGGGTCGCGGGAATGATCGGACTGGAAAAGATG
CTCTGA
Translation:
MSRSFEFPREMSLLRRFLLLALLALSGCASTPPPQPSSALPAEGTWLARQAEIQGRDHPGQSGFHLLGASED
AFVARAALIRAAQRSLDIQYYIVHDGLTTRALAYELLKAADRGVRVRILIDDTASDGWDYEIGVLSAHPNIQ
VRLFNPLHLGRATGITRGVGRLFNLSQQHRRMHNKLWLADGTAAIVGGRNLGDEYFNAKPEMNFTDLDLLGV
GPIANQLSHSFDQYWNSAISRPIEDFLWRAPYPGELESARRKLQRYLRKESVKESGYIRHLFDHGDQPRLGN
WLENLTWARAEAIWDAPLKVLSRGEPDSHLLLSPHLAGLFKGVQKELILVSAYFVPAKDGLNYLTGKADSGV
RVRLLTNSLEATDVPAVHAGYAPYRMALLEHGVKLYELRANPDQPLSGAPWRLHGSSSASLHSKAMVFDRRK
VFIGSFNFDPRSILWNTEVGVIVDSPLLAEQVRQLALEGMAPSVSYQVRIDRSGSRPKLVWIDERDGRAQVL
RHEPGSLWRRLNAWVAGMIGLEKML*
AnnotationID:669GeneID:660
AnnotatorUID: diggins
Modification Date/Time: 2005-03-16 13:01:52
Gene Name:
Confidence Code:3
GeneProduct:putative cardiolipin synthase
Cell Localization:()
Synonyms:PA5310
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:putative phospholipase D
Functional Category Confidence Code:5
COGs:COG1502,cd00138
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70841.1|AE016728_10 (AE016793) phospholipase D family protein [Pseudomonas putida
           KT2440]
          Length = 517

 Score =  630 bits (1626), Expect = e-180
 Identities = 318/513 (61%), Positives = 384/513 (74%), Gaps = 6/513 (1%)

>gb|AAO53649.1| (AE016856) phospholipase D family protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 542

 Score =  615 bits (1586), Expect = e-175
 Identities = 310/522 (59%), Positives = 382/522 (73%), Gaps = 13/522 (2%)








 
Structural Features:
COG1502, Cls,
Phosphatidylserine/phosphatidylglycerophosphate/
cardiolipin synthases and related enzymes 
[Lipid metabolism]
CD-Length = 438 residues,  98.6% aligned
Score =  231 bits (590), Expect = 1e-61
Identities = 144/501 (28%), Positives = 203/501 (40%), Gaps = 76/501 (15%)

cd00138, PLDc, Phospholipase D
CD-Length = 176 residues,  84.7% aligned
Score = 75.2 bits (184), Expect = 2e-14
Identities = 37/165 (22%), Positives = 61/165 (36%), Gaps = 18/165 (10%)







   
Genomic Context:






   
Comment:


















    ReferenceID:1323




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18465




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34795




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:660_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:660
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1114
                           PA5310_tr
                           translation of PA5310
                           529
                           99.24
                           99.43
                           0
                           2733.0
     

     
     
41880
                           gb|AAG08695.1|AE004943_11
                           (AE004943) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           529
                           99.24
                           99.43
                           0
                           2733.0
     

     
     
41881
                           gb|AAN70841.1|AE016728_10
                           (AE016793) phospholipase D family protein [Pseudomonas putida KT2440]
                           534
                           60.48
                           72.84
                           22
                           1634.0
     

     
     
41882
                           gb|AAO53649.1|
                           (AE016856) phospholipase D family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           554
                           56.31
                           69.85
                           37
                           1600.0
     

     
     
41883
                           emb|CAE43360.1|
                           (BX640420) putative phospholipase D protein [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           556
                           41.54
                           55.21
                           59
                           950.0
     

     






      

          
GC ORFID: 43662How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5310Glimmer Score: 

          
Start: 5516130Stop: 5517719

          
Length: 1590


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5310 translation of PA5310Homolog Bit Score 1057.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3928 (ORF: BLASTX 314.0), t_PA5310 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCGGTCGTTCGAGTTTCCACGGGAGATGTCCTTGCTTCGCCGATTCCTCCTGCTGGCCCTCCTGGCC 
CTCTCCGGCTGCGCCAGCACTCCTCCGCCGCAACCGTCGTCGGCACTGCCCGCCGAGGGTACCTGGCTGGCC 
AGGCAGGCCGAAATCCAGGGACGCGACCACCCCGGCCAGTCCGGCTTCCACCTCCTGGGCGCCAGCGAGGAT 
GCCTTCGTCGCCCGCGCGGCGCTGATTCGCGCAGCCCAGCGCAGCCTCGACATCCAGTACTACATCGTCCAC 
GACGGACTGACCACGCGCGCCCTCGCCTACGAGTTGCTCAAGGCAGCCGACCGTGGCGTGCGGGTACGCATC 
CTCATCGACGACACCGCCAGCGATGGCTGGGACTACGAGATCGGTGTGCTCTCCGCCCATCCGAACATCCAG 
GTGCGCCTGTTCAACCCGTTGCACCTGGGCCGCGCCACCGGCATCACGCGAGGCGTCGGCCGCCTGTTCAAC 
CTGTCCCAGCAACACCGGCGCATGCACAACAAGCTGTGGCTGGCCGACGGCACGGCGGCCATCGTCGGCGGA 
CGCAACCTCGGCGACGAGTATTTCAACGCCAAGCCGGAGATGAACTTCACCGACCTCGACCTGCTCGGCGTC 
GGCCCCATCGCCAACCAGCTCTCGCACAGCTTCGACCAGTACTGGAACAGCGCCATCAGCCGGCCGATCGAG 
GATTTCCTCTGGCGCGCGCCCTATCCCGGCGAACTGGAGTCGGCCCGACGCAAGTTGCAGCGCTACCTGCGC 
AAGGAGTCGGTGAAGGAGTCCGGCTACATCCGCCACCTGTTCGACCACGGCGACCAGCCACGCCTGGGCAAC 
TGGCTGGAGAATCTCACCTGGGCACGCGCCGAGGCGATCTGGGATGCGCCGCTGAAAGTATTGAGCCGCGGC 
GAGCCCGACTCGCACCTATTGCTCAGTCCGCACCTGGCGGGCCTGTTCAAGGGCGTGCAGAAGGAACTGATC 
CTGGTCTCGGCGTATTTCGTTCCGGCCAAGGACGGTCTCAACTACCTCACCGGCAAGGCCGACAGCGGCGTC 
CGCGTGCGCCTGCTGACCAACTCCCTGGAAGCCACCGACGTGCCCGCCGTGCATGCCGGCTACGCACCCTAT 
CGGATGGCGCTGCTGGAACATGGCGTGAAGCTCTACGAGCTGCGCGCCAATCCCGACCAGCCGCTGAGCGGC 
GCGCCCTGGCGCCTGCACGGATCGAGCAGCGCCAGCCTGCACAGCAAGGCGATGGTCTTCGACAGGCGCAAG 
GTATTCATCGGTTCGTTCAACTTCGATCCGCGCTCGATCCTGTGGAACACCGAAGTCGGGGTCATCGTCGAC 
AGTCCGCTGCTGGCCGAGCAGGTCCGCCAACTGGCGCTGGAGGGCATGGCCCCGAGCGTCAGCTACCAGGTT 
CGCATCGACCGCAGCGGCAGCCGGCCGAAGCTGGTCTGGATCGACGAACGCGACGGCCGGGCCCAGGTCCTG 
CGCCACGAGCCGGGTAGCCTGTGGCGACGCCTGAACGCCTGGGTCGCGGGAATGATCGGACTGGAAAAGATG 
CTCTGA