ViewGene
Locus Name:
GeneID:
Location:

GeneID:66
TrivialName: PA14_31890
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31890
PAO1 Ortholog Locus:PA2527
Sequence Length:3132
Protein Length:1043
Start: 2773332
Stop: 2776463
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05915.1|AE004680_3 (AE004680) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
Identities = 1043/1043 (100%), Positives = 1043/1043 (100%)
Sequence:
ATGAACCCGTCCCGCCCGTTCATCCTGCGGCCGGTCGCGACCACCCTGCTGATGGTGGCGATCCTGCTCTCG
GGCCTGATCGCCTACCGCTTCCTGCCGATCTCGGCGTTGCCGGAAGTGGACTACCCGACCATCCAGGTGGTC
ACCCTGTACCCCGGTGCCAGCCCGGAGATCATGACCTCGTCGATCACCGCGCCGCTGGAGAACCAGCTCGGG
CAGATTCCGGGGCTCAACGAGATGTCTTCCAGCAGTTCCGGCGGCGCCTCGGTGATCACCCTGCAATTCAGC
CTGCAGAGCAACCTCGATGTCGCCGAGCAGGAAGTCCAGGCGGCGATCAACGCCGCGCAGAGCCTGCTGCCC
AACGACCTGCCGAACCAGCCGGTGTTCAGCAAGGTGAACCCGGCGGACGCACCGATCCTGACCCTGGCGGTG
ATGTCCGATGGCATGCCGCTGCCGCAGATCCAGGACCTGGTGGATACCCGCCTGGCACAGAAGATCTCGCAG
ATCTCCGGGGTCGGCCTGGTCAGCATCAGCGGCGGCCAGCGTCCGGCGGTGCGGGTGCGCGCCAACCCGACG
GCGCTGGCGGCGGCCGGGCTGAGCCTGGAGGACCTGCGCAGCACGGTGACCAGCAACAACCTCAACGGCCCC
AAGGGCAGCTTCGACGGCCCGACCCGTGCCTCGACCCTGGACGCCAACGACCAGTTGCGCTCGGCCGACGCC
TACCGCGACCTGATCATCGCCTACAAGAACGGCTCGCCGCTGCGCATCCGCGACGTCGCCAGCGTCGAGGAC
GACGCCGAGAACGTGCGCCTGGCCGCCTGGGCCAACAACCTGCCGGCGGTGGTGCTGAACATCCAGCGCCAG
CCGGGGGCCAACGTGATCGAGGTGGTCGACCGGATCAAGGCGCTGCTGCCGCAGCTGCAATCGACCCTGCCG
GGGAATCTCGACGTGCAGGTGCTGACCGACCGCACCACCACCATCCGCGCCTCGGTCAAGGACGTGCAGTTC
GAGCTGGCGCTGGCGGTGGCGCTGGTGGTGATGGTCACCTTCCTGTTCCTGCGCAACGTCTACGCCACCCTG
ATTCCCAGCTTCGCCGTGCCGCTGTCGCTGATCGGCACCTTCGGCGTGATGTACCTGTCCGGCTTCTCGATC
AACAACCTGACCCTGATGGCGCTGACCATCGCCACCGGCTTCGTGGTCGACGACGCGATCGTCATGGTGGAG
AACATCGCCCGCTACCTGGAGCAGGGCGACTCGCCGCTGGAAGCGGCGCTCAAGGGCTCGAAGCAGATCGGC
TTCACCATCATCTCGCTGACCTTCTCGCTGATCGCCGTGCTGATCCCGCTGCTGTTCATGGGCGACGTCGCC
GGGCGGCTGTTCCGCGAGTTCGCCATCACCCTGGCGGTGGCGATCCTGATTTCCGGCTTCGTCTCCCTGACC
CTTACGCCGATGCTCAGCGCCAAGCTGCTGCGCCACATCGACGAGGACCAGCAGGGCCGCTTCGCGCGCGCC
GCGGGGCGGGTCATCGATGGCCTGATCGCACAATACGCCAAGGCCCTGCGGGTGGTCCTGCGGCACCAGCCG
CTGACCCTGCTGGTGGCCATCGCCACCCTGGCGCTGACCGCGCTGCTCTACCTGGCCATGCCCAAGGGCTTC
TTCCCGGTGCAGGACACCGGAGTGATCCAGGGCGTCGCCGAAGCGCCGCAGTCGATCTCCTTCCAGGCCATG
TCCGAGCGCCAGCGCGCCCTTGCCGAGGTGGTGCTGAAGGACCCGGCGGTGGCCAGCCTGTCCTCCTACATC
GGCGTCGACGGCAGCAACCCGACCCTCAACACCGGCCGCCTGCTGATCAACCTCAAGCCGCACAGCGAGCGC
GACGTCACCGCCAGCGAAGTGATCCAGCGCCTGCAGCCCGAACTCGACCATCTGCCCGGGATCAAGCTGTAC
ATGCAGCCGGTGCAGGACCTGACCATCGAGGACCGGGTCGCCCGCACCCAGTACCAGTTCACCTTGCAGGAC
GCCGACCCGGACGTGCTCGCCGAGTGGGTGCCGAAGCTGGTGGCGCGGCTGCAGGAGTTGCCGCAGCTCGCC
GACGTCGCCAGCGACTGGCAGGACAAGGGCTTGCAGGCCTACCTGAACATCGACCGCGACACCGCATCGCGC
CTCGGCGTGAAGCTCTCCGACATCGACAGCGTGCTCTACAACGCCTTCGGCCAGCGGCTGATCTCGACCATC
TTCACCCAGGCCACCCAGTACCGCGTGGTGCTGGAGGTGGCGCCGCAGTTCCAGCTCGGCCCGCAGGCCCTG
GAGCAGCTCTACGTGCCGTCCAGCGACGGCACCCAGGTGCGCCTGTCGAGCCTGGCGAAGGTGGAGGAGCGG
CATACCCTGCTGGCGATCAACCATATCGCCCAGTTCCCCTCGGCGACCCTGTCGTTCAACCTGGCCAAGGGT
TACTCCCTGGGCGAGGCGGTCGAGGCGATCCGTGGCGTCGAGGCCAGCCTGGAGCTGCCGCTGAGCATGCAG
GGCAGCTTCCGCGGCGCGGCGCTGGCCTTCGAGGCCTCGCTGTCGAACACGCTGCTGCTGATCCTCGCCTCG
GTGGTGACCATGTACATCGTCCTGGGCATCCTCTACGAGAGCTTCATCCATCCGGTGACCATCCTCTCGACC
CTGCCCTCGGCCGGGGTCGGCGCGCTGCTGGCGCTGATGCTGGCGGGGCAGGAGATCGGCATCGTGGCGATC
ATCGGCATCATCCTGCTGATCGGCATCGTCAAGAAGAACGCGATCATGATGATCGACTTCGCCCTCGACGCC
GAGCGCAACGAAGGCAAGCCGCCCCATGAGGCGATCTACCAGGCCTGCCTGCTGCGCTTCCGGCCGATCCTG
ATGACCACCATGGCCGCGCTGCTCGGCGCGCTGCCGCTGATGCTCGCCGGCGGCGCCGGCGCCGAGCTGCGC
CAGCCGCTGGGCATCACCATGGTCGGTGGCCTGCTGCTGAGCCAGGTCCTGACCCTGTTCACCACCCCGGTG
ATCTATCTCTACTTCGACCGCCTGGCCCGTCGCTGGGCGGCCTGGCGCAAGCAGCGCGGGCTGGACCTGAAC
ACCGAGGCCGGGTTCGACGGGGATGCCGGGCGATGA
Translation:
MNPSRPFILRPVATTLLMVAILLSGLIAYRFLPISALPEVDYPTIQVVTLYPGASPEIMTSSITAPLENQLG
QIPGLNEMSSSSSGGASVITLQFSLQSNLDVAEQEVQAAINAAQSLLPNDLPNQPVFSKVNPADAPILTLAV
MSDGMPLPQIQDLVDTRLAQKISQISGVGLVSISGGQRPAVRVRANPTALAAAGLSLEDLRSTVTSNNLNGP
KGSFDGPTRASTLDANDQLRSADAYRDLIIAYKNGSPLRIRDVASVEDDAENVRLAAWANNLPAVVLNIQRQ
PGANVIEVVDRIKALLPQLQSTLPGNLDVQVLTDRTTTIRASVKDVQFELALAVALVVMVTFLFLRNVYATL
IPSFAVPLSLIGTFGVMYLSGFSINNLTLMALTIATGFVVDDAIVMVENIARYLEQGDSPLEAALKGSKQIG
FTIISLTFSLIAVLIPLLFMGDVAGRLFREFAITLAVAILISGFVSLTLTPMLSAKLLRHIDEDQQGRFARA
AGRVIDGLIAQYAKALRVVLRHQPLTLLVAIATLALTALLYLAMPKGFFPVQDTGVIQGVAEAPQSISFQAM
SERQRALAEVVLKDPAVASLSSYIGVDGSNPTLNTGRLLINLKPHSERDVTASEVIQRLQPELDHLPGIKLY
MQPVQDLTIEDRVARTQYQFTLQDADPDVLAEWVPKLVARLQELPQLADVASDWQDKGLQAYLNIDRDTASR
LGVKLSDIDSVLYNAFGQRLISTIFTQATQYRVVLEVAPQFQLGPQALEQLYVPSSDGTQVRLSSLAKVEER
HTLLAINHIAQFPSATLSFNLAKGYSLGEAVEAIRGVEASLELPLSMQGSFRGAALAFEASLSNTLLLILAS
VVTMYIVLGILYESFIHPVTILSTLPSAGVGALLALMLAGQEIGIVAIIGIILLIGIVKKNAIMMIDFALDA
ERNEGKPPHEAIYQACLLRFRPILMTTMAALLGALPLMLAGGAGAELRQPLGITMVGGLLLSQVLTLFTTPV
IYLYFDRLARRWAAWRKQRGLDLNTEAGFDGDAGR*
AnnotationID:76GeneID:66
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 18:06:14
Gene Name:
Confidence Code:3
GeneProduct:putative RND efflux transporter
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putative drug efflux pump transmembrane protein
Functional Category Confidence Code:5
COGs:COG0841,pfam00873,COG3696
Secondary Functional Category(ies):membrane proteins
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD18349.1| (AL646083) PROBABLE DRUG EFFLUX PUMP TRANSMEMBRANE PROTEIN [Ralstonia
            solanacearum]
          Length = 1041

 Score = 1456 bits (3770), Expect = 0.0
 Identities = 749/1027 (72%), Positives = 880/1027 (85%)

>emb|CAE39241.1| (BX640435) AcrB/AcrD/AcrF family protein [Bordetella parapertussis]
          Length = 1033

 Score = 1367 bits (3539), Expect = 0.0
 Identities = 705/1021 (69%), Positives = 853/1021 (83%), Gaps = 2/1021 (0%)

>dbj|BAC06608.1| (AB089188) multidrug transporter [Escherichia coli]
          Length = 1040

 Score = 1336 bits (3458), Expect = 0.0
 Identities = 685/1025 (66%), Positives = 841/1025 (82%), Gaps = 4/1025 (0%)






 
Structural Features:
COG0841, AcrB, Cation/multidrug efflux pump [Defense mechanisms]
CD-Length = 1009 residues, 100.0% aligned
Score =  962 bits (2489), Expect = 0.0
Identities = 401/1018 (39%), Positives = 595/1018 (58%), Gaps = 12/1018 (1%)

pfam00873, ACR_tran, AcrB/AcrD/AcrF family. 
CD-Length = 1017 residues,  99.9% aligned
Score =  908 bits (2347), Expect = 0.0
Identities = 391/1020 (38%), Positives = 586/1020 (57%), Gaps = 13/1020 (1%)

COG3696, COG3696, Putative silver efflux pump 
[Inorganic ion transport and metabolism]
CD-Length = 1027 residues,  98.2% aligned
Score =  469 bits (1208), Expect = 8e-133
Identities = 286/1020 (28%), Positives = 481/1020 (47%), Gaps = 25/1020 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:135




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17277




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33089




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:66_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:66
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
135
                           PA2527_tr
                           translation of PA2527
                           1043
                           99.99
                           99.99
                           0
                           5120.0
     

     
     
13254
                           gb|AAG05915.1|AE004680_3
                           (AE004680) probable RND efflux transporter [Pseudomonas aeruginosa PAO1]
                           1043
                           99.99
                           99.99
                           0
                           5120.0
     

     
     
13255
                           emb|CAD18349.1|
                           (AL646083) PROBABLE DRUG EFFLUX PUMP TRANSMEMBRANE PROTEIN [Ralstonia solanacearum]
                           1044
                           71.93
                           84.57
                           4
                           3766.0
     

     
     
13256
                           gb|AAQ60403.1|
                           (AE016919) probable drug efflux pump transmembrane protein [Chromobacterium violaceum ATCC 12472]
                           1043
                           71.62
                           85.04
                           10
                           3750.0
     

     
     
13258
                           emb|CAE39241.1|
                           (BX640435) AcrB/AcrD/AcrF family protein [Bordetella parapertussis]
                           1048
                           67.65
                           81.77
                           20
                           3537.0
     

     






      

          
GC ORFID: 43068How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2527Glimmer Score: 

          
Start: 2180594Stop: 2177463

          
Length: 3132


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2527 translation of PA2527Homolog Bit Score 1976.0

          
Other Homologs:  t_PA2526 (ORF: BLASTX 998.0), psyr_15may02_Scaffold1_revised_gene1404 (ORF: BLASTX 909.0), t_PA2527 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACCCGTCCCGCCCGTTCATCCTGCGGCCGGTCGCGACCACCCTGCTGATGGTGGCGATCCTGCTCTCG 
GGCCTGATCGCCTACCGCTTCCTGCCGATCTCGGCGTTGCCGGAAGTGGACTACCCGACCATCCAGGTGGTC 
ACCCTGTACCCCGGTGCCAGCCCGGAGATCATGACCTCGTCGATCACCGCGCCGCTGGAGAACCAGCTCGGG 
CAGATTCCGGGGCTCAACGAGATGTCTTCCAGCAGTTCCGGCGGCGCCTCGGTGATCACCCTGCAATTCAGC 
CTGCAGAGCAACCTCGATGTCGCCGAGCAGGAAGTCCAGGCGGCGATCAACGCCGCGCAGAGCCTGCTGCCC 
AACGACCTGCCGAACCAGCCGGTGTTCAGCAAGGTGAACCCGGCGGACGCACCGATCCTGACCCTGGCGGTG 
ATGTCCGATGGCATGCCGCTGCCGCAGATCCAGGACCTGGTGGATACCCGCCTGGCACAGAAGATCTCGCAG 
ATCTCCGGGGTCGGCCTGGTCAGCATCAGCGGCGGCCAGCGTCCGGCGGTGCGGGTGCGCGCCAACCCGACG 
GCGCTGGCGGCGGCCGGGCTGAGCCTGGAGGACCTGCGCAGCACGGTGACCAGCAACAACCTCAACGGCCCC 
AAGGGCAGCTTCGACGGCCCGACCCGTGCCTCGACCCTGGACGCCAACGACCAGTTGCGCTCGGCCGACGCC 
TACCGCGACCTGATCATCGCCTACAAGAACGGCTCGCCGCTGCGCATCCGCGACGTCGCCAGCGTCGAGGAC 
GACGCCGAGAACGTGCGCCTGGCCGCCTGGGCCAACAACCTGCCGGCGGTGGTGCTGAACATCCAGCGCCAG 
CCGGGGGCCAACGTGATCGAGGTGGTCGACCGGATCAAGGCGCTGCTGCCGCAGCTGCAATCGACCCTGCCG 
GGGAATCTCGACGTGCAGGTGCTGACCGACCGCACCACCACCATCCGCGCCTCGGTCAAGGACGTGCAGTTC 
GAGCTGGCGCTGGCGGTGGCGCTGGTGGTGATGGTCACCTTCCTGTTCCTGCGCAACGTCTACGCCACCCTG 
ATTCCCAGCTTCGCCGTGCCGCTGTCGCTGATCGGCACCTTCGGCGTGATGTACCTGTCCGGCTTCTCGATC 
AACAACCTGACCCTGATGGCGCTGACCATCGCCACCGGCTTCGTGGTCGACGACGCGATCGTCATGGTGGAG 
AACATCGCCCGCTACCTGGAGCAGGGCGACTCGCCGCTGGAAGCGGCGCTCAAGGGCTCGAAGCAGATCGGC 
TTCACCATCATCTCGCTGACCTTCTCGCTGATCGCCGTGCTGATCCCGCTGCTGTTCATGGGCGACGTCGCC 
GGGCGGCTGTTCCGCGAGTTCGCCATCACCCTGGCGGTGGCGATCCTGATTTCCGGCTTCGTCTCCCTGACC 
CTTACGCCGATGCTCAGCGCCAAGCTGCTGCGCCACATCGACGAGGACCAGCAGGGCCGCTTCGCGCGCGCC 
GCGGGGCGGGTCATCGATGGCCTGATCGCACAATACGCCAAGGCCCTGCGGGTGGTCCTGCGGCACCAGCCG 
CTGACCCTGCTGGTGGCCATCGCCACCCTGGCGCTGACCGCGCTGCTCTACCTGGCCATGCCCAAGGGCTTC 
TTCCCGGTGCAGGACACCGGAGTGATCCAGGGCGTCGCCGAAGCGCCGCAGTCGATCTCCTTCCAGGCCATG 
TCCGAGCGCCAGCGCGCCCTTGCCGAGGTGGTGCTGAAGGACCCGGCGGTGGCCAGCCTGTCCTCCTACATC 
GGCGTCGACGGCAGCAACCCGACCCTCAACACCGGCCGCCTGCTGATCAACCTCAAGCCGCACAGCGAGCGC 
GACGTCACCGCCAGCGAAGTGATCCAGCGCCTGCAGCCCGAACTCGACCATCTGCCCGGGATCAAGCTGTAC 
ATGCAGCCGGTGCAGGACCTGACCATCGAGGACCGGGTCGCCCGCACCCAGTACCAGTTCACCTTGCAGGAC 
GCCGACCCGGACGTGCTCGCCGAGTGGGTGCCGAAGCTGGTGGCGCGGCTGCAGGAGTTGCCGCAGCTCGCC 
GACGTCGCCAGCGACTGGCAGGACAAGGGCTTGCAGGCCTACCTGAACATCGACCGCGACACCGCATCGCGC 
CTCGGCGTGAAGCTCTCCGACATCGACAGCGTGCTCTACAACGCCTTCGGCCAGCGGCTGATCTCGACCATC 
TTCACCCAGGCCACCCAGTACCGCGTGGTGCTGGAGGTGGCGCCGCAGTTCCAGCTCGGCCCGCAGGCCCTG 
GAGCAGCTCTACGTGCCGTCCAGCGACGGCACCCAGGTGCGCCTGTCGAGCCTGGCGAAGGTGGAGGAGCGG 
CATACCCTGCTGGCGATCAACCATATCGCCCAGTTCCCCTCGGCGACCCTGTCGTTCAACCTGGCCAAGGGT 
TACTCCCTGGGCGAGGCGGTCGAGGCGATCCGTGGCGTCGAGGCCAGCCTGGAGCTGCCGCTGAGCATGCAG 
GGCAGCTTCCGCGGCGCGGCGCTGGCCTTCGAGGCCTCGCTGTCGAACACGCTGCTGCTGATCCTCGCCTCG 
GTGGTGACCATGTACATCGTCCTGGGCATCCTCTACGAGAGCTTCATCCATCCGGTGACCATCCTCTCGACC 
CTGCCCTCGGCCGGGGTCGGCGCGCTGCTGGCGCTGATGCTGGCGGGGCAGGAGATCGGCATCGTGGCGATC 
ATCGGCATCATCCTGCTGATCGGCATCGTCAAGAAGAACGCGATCATGATGATCGACTTCGCCCTCGACGCC 
GAGCGCAACGAAGGCAAGCCGCCCCATGAGGCGATCTACCAGGCCTGCCTGCTGCGCTTCCGGCCGATCCTG 
ATGACCACCATGGCCGCGCTGCTCGGCGCGCTGCCGCTGATGCTCGCCGGCGGCGCCGGCGCCGAGCTGCGC 
CAGCCGCTGGGCATCACCATGGTCGGTGGCCTGCTGCTGAGCCAGGTCCTGACCCTGTTCACCACCCCGGTG 
ATCTATCTCTACTTCGACCGCCTGGCCCGTCGCTGGGCGGCCTGGCGCAAGCAGCGCGGGCTGGACCTGAAC 
ACCGAGGCCGGGTTCGACGGGGATGCCGGGCGATGA