ViewGene
Locus Name:
GeneID:
Location:

GeneID:658
TrivialName: PA14_46120
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_46120
PAO1 Ortholog Locus:PA1417
Sequence Length:1602
Protein Length:533
Start: 4101481
Stop: 4103082
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04806.1|AE004571_5 (AE004571) probable decarboxylase [Pseudomonas aeruginosa PAO1]
Identities = 531/533 (99%), Positives = 531/533 (99%)
Sequence:
ATGACCACCTGCGGCGAATTCCTCGTCAAGCAACTCGAAGCCTGGGGCGTCGAGACCGTCTTCGGCATCCCC
GGCGTACATACCGTAGAACTCTACCGCGGCCTGCCCGGCAGCCGTATCCGCCATGTCACCCCGCGCCACGAG
CAGGGCGCCGGCTTCATGGCGGACGGCTACGCACGGGTCACCGGCAAGCCCGGCGTGTGCTTCATCATCACC
GGCCCGGGGATGACCAACATCCTCACCGCCATGGCCCAGGCCTACGCCGACTCGATTCCGATGCTGGTGATC
TCCAGCGTCAACGAACGCGCGCGCCTGGCCCACGGCAACGGCTACCTGCACGAGCTGCCGAACCAGCGCAAC
CTGGTAGGCAACGTCTGCGCCTTCAGCCATACCCTGATGAGCGCCGAGGAGCTGCCGGCGGTGCTCGCCCGC
GCCTTCGCCGTGTTCGACAGCGAGCGGCCGCGGCCGGTGCACATCGAGCTGCCGCTCGACGTCATCACCGCG
CCCGCCGAACACCTGGCCCTGCGCCCGCGCACCGTCGCCAGCCGCCCGGCGCCGGCACTCGCGCCCTTGCGC
GAGGCCGCGGCGCGCCTGCGCAACGCGAAGAAGCCACTGCTGCTGCTCGGCGGCGGCTGCGTCGAGGCCGCC
GCCGAAGCGCGTGCCCTGGCCGCCGCGCTGGACGCGCCGACCGCCCTGACGATCAACGCCAAGGGCCTGCTA
CCGGCCGACCACCCGCTGCTGCTGGGCAGCAACCAGTCCTGCGTGCCGGTACGCGAGCTGGCCGCCGAGGCC
GACGTGGTGCTGGCCATCGGCACCGAGCTGGGCGAAACCGACTACGACGTGGTGTTCGACGGCGGCTTCGCC
CTCGATGGCGAGTTGATCCGCATCGACATCGACCCGCAGCAACTGATGCGCAACTACACGCCTAGCCTGGCC
ATCCACAGCGACGCCCGGCTGGCCATGCGCGTGCTGCTGGCCGAACTGCCGGGCGGCGAGGCCTCGCCGGAC
AGCCCCGGGGCCCGGCGCGCCGCCGCCGTGCGCCAGCGCCTGGCCGAAGACTTCGCCGGCTGGTCGCACTAT
CGCCAGCTGTTCGCCGCGATCCTCGCCGAGTGGCCGGACGCCCGCTTCGTCGGCGACTCGACCCAGACCGTC
TACAGCGGCAACCACCTGGTCGATCTCGACGAGCCGCGCCGCTGGTTCAACGCCTCCACCGGCTACGGCACC
CTCGGCTATGGCCTGCCCGCCGCCATCGGCGCCAAGCTCGGCGAGCCGGGACGCCCGGTGGTCAGCCTGATG
GGCGACGGCGGCCTGCAGTTCACCCTGCCGGAACTGGCCAGCGCGGTGGAAGCCAAGGTCGGCATCGTCGTG
CTGCTGTGGAACAACCACGGCTATGGCGAGATCAAGCGCTACATGGAGCGGCGCGAGATCACTCCGCTCGGG
GTCGATATCTACACCCCGGACTTCCTCGCCATCGCCCGCGGCTTCGGCTGCGCCGCCGAACGCGCGCGCGAT
CTCGAGCACCTGCGCGAACTGCTGCGCGGCGCCCCGGCGGACCGTCCGCTGATCGTCGAGGTGCTGCAAGCC
GCGCCCTTCCATCCCTGA
Translation:
MTTCGEFLVKQLEAWGVETVFGIPGVHTVELYRGLPGSRIRHVTPRHEQGAGFMADGYARVTGKPGVCFIIT
GPGMTNILTAMAQAYADSIPMLVISSVNERARLAHGNGYLHELPNQRNLVGNVCAFSHTLMSAEELPAVLAR
AFAVFDSERPRPVHIELPLDVITAPAEHLALRPRTVASRPAPALAPLREAAARLRNAKKPLLLLGGGCVEAA
AEARALAAALDAPTALTINAKGLLPADHPLLLGSNQSCVPVRELAAEADVVLAIGTELGETDYDVVFDGGFA
LDGELIRIDIDPQQLMRNYTPSLAIHSDARLAMRVLLAELPGGEASPDSPGARRAAAVRQRLAEDFAGWSHY
RQLFAAILAEWPDARFVGDSTQTVYSGNHLVDLDEPRRWFNASTGYGTLGYGLPAAIGAKLGEPGRPVVSLM
GDGGLQFTLPELASAVEAKVGIVVLLWNNHGYGEIKRYMERREITPLGVDIYTPDFLAIARGFGCAAERARD
LEHLRELLRGAPADRPLIVEVLQAAPFHP*
AnnotationID:667GeneID:658
AnnotatorUID: diggins
Modification Date/Time: 2005-05-26 18:06:26
Gene Name:
Confidence Code:3
GeneProduct:putative decarboxylase
Cell Localization:()
Synonyms:ilvG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:putative acetolactate synthase
Functional Category Confidence Code:5
COGs:COG0028,COG3960,COG3961
Secondary Functional Category(ies):coenzyme metabolism
EC Number:2.2.1.6
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67017.1|AE016330_6 (AE016779) acetolactate synthase, large subunit, putative
           [Pseudomonas putida KT2440]
          Length = 545

 Score =  612 bits (1577), Expect = e-174
 Identities = 333/543 (61%), Positives = 382/543 (70%), Gaps = 23/543 (4%)

>emb|CAC45139.1| (AL591784) PUTATIVE ACETOLACTATE SYNTHASE ISOZYME I LARGE SUBUNIT
           PROTEIN [Sinorhizobium meliloti]
          Length = 537

 Score =  485 bits (1249), Expect = e-136
 Identities = 275/535 (51%), Positives = 339/535 (63%), Gaps = 22/535 (4%)

>dbj|BAB51695.1| (AP003006) acetohydroxy acid synthase [Mesorhizobium loti]
          Length = 526

 Score =  473 bits (1218), Expect = e-132
 Identities = 270/529 (51%), Positives = 336/529 (63%), Gaps = 10/529 (1%)






 
Structural Features:
COG0028, IlvB, Thiamine pyrophosphate-requiring enzymes 
[acetolactate synthase, pyruvate dehydrogenase (cytochrome), 
glyoxylate carboligase, phosphonopyruvate decarboxylase]
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 550 residues,  96.0% aligned
Score =  440 bits (1134), Expect = 1e-124
Identities = 206/535 (38%), Positives = 278/535 (51%), Gaps = 17/535 (3%)

COG3960, COG3960, Glyoxylate carboligase.
CD-Length = 592 residues,  91.4% aligned
Score =  199 bits (508), Expect = 5e-52
Identities = 162/551 (29%), Positives = 245/551 (44%), Gaps = 46/551 (8%)

COG3961, COG3961, Pyruvate decarboxylase and related 
thiamine pyrophosphate-requiring enzymes 
[Carbohydrate transport and metabolism / Coenzyme metabolism]
CD-Length = 557 residues,  95.2% aligned
Score =  159 bits (404), Expect = 7e-40
Identities = 146/561 (26%), Positives = 225/561 (40%), Gaps = 67/561 (11%)






   
Genomic Context:






   
Comment:
other possible gene name: ilvB


















    ReferenceID:1319




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18461




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37432




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:658_cdd.html


    		




 
URL:

















    ReferenceID:38176




  
Author/Investigator(s):
        Cox JL, Cox BJ, Fidanza V, Calhoun DH.





  
Title:
        The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12.




  
PubMed:
        3315862


MedLine:
        




    
Source:
Gene. 1987;56(2-3):185-98.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3315862&query_hl=33

















    ReferenceID:38177




  
Author/Investigator(s):
        Hauser CA, Hatfield GW.





  
Title:
        Nucleotide sequence of the ilvB multivalent attenuator region of Escherichia coli K12.




  
PubMed:
        6346263


MedLine:
        




    
Source:
Nucleic Acids Res. 1983 Jan 11;11(1):127-39.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6346263









           
Homologs By Global Alignment 

           

       



     
Gene ID:658
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1111
                           PA1417_tr
                           translation of PA1417
                           533
                           99.62
                           99.62
                           0
                           2742.0
     

     
     
41874
                           gb|AAG04806.1|AE004571_5
                           (AE004571) probable decarboxylase [Pseudomonas aeruginosa PAO1]
                           533
                           99.62
                           99.62
                           0
                           2742.0
     

     
     
41875
                           gb|AAN67017.1|AE016330_6
                           (AE016779) acetolactate synthase, large subunit, putative [Pseudomonas putida KT2440]
                           554
                           61.01
                           69.85
                           30
                           1594.0
     

     
     
41877
                           dbj|BAB51695.1|
                           (AP003006) acetohydroxy acid synthase [Mesorhizobium loti]
                           545
                           49.72
                           61.65
                           31
                           1234.0
     

     
     
41876
                           emb|CAC45139.1|
                           (AL591784) PUTATIVE ACETOLACTATE SYNTHASE ISOZYME I LARGE SUBUNIT PROTEIN [Sinorhizobium meliloti]
                           556
                           49.46
                           61.51
                           42
                           1260.0
     

     






      

          
GC ORFID: 43660How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1417Glimmer Score: 

          
Start: 852428Stop: 850827

          
Length: 1602


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1417 translation of PA1417Homolog Bit Score 1060.0

          
Other Homologs:  t_PA1417 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCACCTGCGGCGAATTCCTCGTCAAGCAACTCGAAGCCTGGGGCGTCGAGACCGTCTTCGGCATCCCC 
GGCGTACATACCGTAGAACTCTACCGCGGCCTGCCCGGCAGCCGTATCCGCCATGTCACCCCGCGCCACGAG 
CAGGGCGCCGGCTTCATGGCGGACGGCTACGCACGGGTCACCGGCAAGCCCGGCGTGTGCTTCATCATCACC 
GGCCCGGGGATGACCAACATCCTCACCGCCATGGCCCAGGCCTACGCCGACTCGATTCCGATGCTGGTGATC 
TCCAGCGTCAACGAACGCGCGCGCCTGGCCCACGGCAACGGCTACCTGCACGAGCTGCCGAACCAGCGCAAC 
CTGGTAGGCAACGTCTGCGCCTTCAGCCATACCCTGATGAGCGCCGAGGAGCTGCCGGCGGTGCTCGCCCGC 
GCCTTCGCCGTGTTCGACAGCGAGCGGCCGCGGCCGGTGCACATCGAGCTGCCGCTCGACGTCATCACCGCG 
CCCGCCGAACACCTGGCCCTGCGCCCGCGCACCGTCGCCAGCCGCCCGGCGCCGGCACTCGCGCCCTTGCGC 
GAGGCCGCGGCGCGCCTGCGCAACGCGAAGAAGCCACTGCTGCTGCTCGGCGGCGGCTGCGTCGAGGCCGCC 
GCCGAAGCGCGTGCCCTGGCCGCCGCGCTGGACGCGCCGACCGCCCTGACGATCAACGCCAAGGGCCTGCTA 
CCGGCCGACCACCCGCTGCTGCTGGGCAGCAACCAGTCCTGCGTGCCGGTACGCGAGCTGGCCGCCGAGGCC 
GACGTGGTGCTGGCCATCGGCACCGAGCTGGGCGAAACCGACTACGACGTGGTGTTCGACGGCGGCTTCGCC 
CTCGATGGCGAGTTGATCCGCATCGACATCGACCCGCAGCAACTGATGCGCAACTACACGCCTAGCCTGGCC 
ATCCACAGCGACGCCCGGCTGGCCATGCGCGTGCTGCTGGCCGAACTGCCGGGCGGCGAGGCCTCGCCGGAC 
AGCCCCGGGGCCCGGCGCGCCGCCGCCGTGCGCCAGCGCCTGGCCGAAGACTTCGCCGGCTGGTCGCACTAT 
CGCCAGCTGTTCGCCGCGATCCTCGCCGAGTGGCCGGACGCCCGCTTCGTCGGCGACTCGACCCAGACCGTC 
TACAGCGGCAACCACCTGGTCGATCTCGACGAGCCGCGCCGCTGGTTCAACGCCTCCACCGGCTACGGCACC 
CTCGGCTATGGCCTGCCCGCCGCCATCGGCGCCAAGCTCGGCGAGCCGGGACGCCCGGTGGTCAGCCTGATG 
GGCGACGGCGGCCTGCAGTTCACCCTGCCGGAACTGGCCAGCGCGGTGGAAGCCAAGGTCGGCATCGTCGTG 
CTGCTGTGGAACAACCACGGCTATGGCGAGATCAAGCGCTACATGGAGCGGCGCGAGATCACTCCGCTCGGG 
GTCGATATCTACACCCCGGACTTCCTCGCCATCGCCCGCGGCTTCGGCTGCGCCGCCGAACGCGCGCGCGAT 
CTCGAGCACCTGCGCGAACTGCTGCGCGGCGCCCCGGCGGACCGTCCGCTGATCGTCGAGGTGCTGCAAGCC 
GCGCCCTTCCATCCCTGA