ViewGene
Locus Name:
GeneID:
Location:

GeneID:6494
TrivialName: PA14_13940
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_13940
PAO1 Ortholog Locus:
Sequence Length:1680
Protein Length:559
Start: 1196245
Stop: 1197924
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07253.1|AE004804_1 (AE004804) pyocin protein [Pseudomonas aeruginosa PAO1]
Identities = 555/559 (99%), Positives = 557/559 (99%)
Sequence:
ATGGAGGCGCAAGCCCAGCAGGTCACTCAACAGCTCGGTGCCGACTTCAACGCGATCACCACTCCGACCGCC
ACCAAGGTCCAGCAACGTGTGAAAGCAGTGGATGCTAGTCTGTCTCAGGTTTCCACCCAGGTCAGTGGTGCA
GTCGCCTCGGCAACCCAGGCGGTGCAGGTCAAGACAGCGCAAGCCCAGCAACAAGCCAACAGCCAGATTTCG
TCCAGCCAGAACCTGATCAGCGCGGCATTTCGGAATCAGATTGCCTTGGCAGCACAGGCCAGTGGAGAAGCC
CAGGTCACGGCTTTCAACACCAAAGTCAAGCAGATCGTCAACGAAGCCACGGCGTTTTCGACAGCACGAAAA
CAAGCGCTGGCTCAGTTTGCTGCCAATGCGGCCGCCGAGGTCGAAACCGAGGTCAACAGGCTAACCGCCCAG
GTCAAGGCCAGCCCCACCAAGGCGGCTTCGCAAGCTGCACAAGCAACCCTTCGACAATTCACTGAAAGCACC
TATGCCAAGGTGAACACCTATGCCCAGCAGACCGAAGCAGCGCTGCAGGCCAAAGCCAAGGAGGTTTCGGCA
GCGGTAGCCGAGGCACAGCGTGCGGCGACCAACGATTTGCAAAAAACAGCCGATGTAGTACCAGGTCAGATC
ATTCAGGCTGCGAACACCTTGGCGATGCCGGCTACCTCTACGCCGCTGGTTTTGGTGGCCGGGTTTGGTAGT
GCGGCAGTGGAGACGGCTCGCCTGGCGGCTTCCCTAACGAATGCTGTCAATCGCCTGGTTCAGATTGCTGGT
AGTGGACCCGGCGCATACGTTGCCACCTTCGCGGTGCTGTCGCTGTATTCCGACCAGGCTGGTAAAGACAGT
GACAAGGTTCCTGCCGGCGTTCGCAATGCATTGGCACTGGAGGCGTCCGCTCTGGGGCTTCCTGGCACGGCT
GATTTGCAAAGCGTCGCCAAGGCAGGTGGCACGGTTGATATGCCGGTACGACTCACGAGTGCTGCACAAGAG
AGCCCGAGTGGTAAATCGCAGATTGCCGCGATGTTGACCAACGGTGCAACTGTCCCCAAGGGCGTGCCTGTT
CGCGCCGCGACCCTCAATGCTGCGACGGGCCGGTATGAGGTGACGGTTCCCGCAAAGTCCACCGTGCCGAAT
ACACCACCGCTGATCTTGACCTGGACCCCTGCCACCCCTCCAGGAAGCCAGAACCCCTCAAGCACCACTCCG
GTCGTACCGCAGCCGGTTCCGGTGTATGAGGGAGCAACGATCACCCCAGTACAGGCCGAGCCGGAGTCCTAT
CCGGGAGTGCCGTTAGATCTGGACGATCTGATTGTGATCTTCCCGGTGGGCTCGGGGGTTAAGCCAATCTAT
ATAATGTTCAATCATAACCCTCATGATGTTGTTATTGATGATAAGATCAGAGAGCAAATTCCAATTAGAGGG
TGGACTGAGGTTGAAATTAGAGATGTTATCGGAAAAGGTGCAACAGGTAGTTCTTCAGATAAGCGTCGGCCT
AATAAGACGATAGATGGGCTGGGGCGGAATGACTCTGCAACAGTATATGGAAGTCCTCCTAATGGATATATA
GTTGTGAACGATAGGACAGGTGAAATTATTCAAATTGCAGATCGGAACGATTCCGCATGGGCGGTTGACGGG
CGAATCATCTGGAGAAATAAGTAA
Translation:
MEAQAQQVTQQLGADFNAITTPTATKVQQRVKAVDASLSQVSTQVSGAVASATQAVQVKTAQAQQQANSQIS
SSQNLISAAFRNQIALAAQASGEAQVTAFNTKVKQIVNEATAFSTARKQALAQFAANAAAEVETEVNRLTAQ
VKASPTKAASQAAQATLRQFTESTYAKVNTYAQQTEAALQAKAKEVSAAVAEAQRAATNDLQKTADVVPGQI
IQAANTLAMPATSTPLVLVAGFGSAAVETARLAASLTNAVNRLVQIAGSGPGAYVATFAVLSLYSDQAGKDS
DKVPAGVRNALALEASALGLPGTADLQSVAKAGGTVDMPVRLTSAAQESPSGKSQIAAMLTNGATVPKGVPV
RAATLNAATGRYEVTVPAKSTVPNTPPLILTWTPATPPGSQNPSSTTPVVPQPVPVYEGATITPVQAEPESY
PGVPLDLDDLIVIFPVGSGVKPIYIMFNHNPHDVVIDDKIREQIPIRGWTEVEIRDVIGKGATGSSSDKRRP
NKTIDGLGRNDSATVYGSPPNGYIVVNDRTGEIIQIADRNDSAWAVDGRIIWRNK*
AnnotationID:6495GeneID:6494
AnnotatorUID: danlee
Modification Date/Time: 2006-03-20 14:02:36
Gene Name:
Confidence Code:3
GeneProduct:putative S-type pyocin protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam06958
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54566.1| (AE016859) colicin/pyosin nuclease family protein [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 459

 Score =  118 bits (296), Expect = 9e-26
 Identities = 108/340 (31%), Positives = 158/340 (46%), Gaps = 41/340 (12%)








 
Structural Features:
pfam06958, Pyocin_S, S-type Pyocin. S-type pyocins cause cell death by 
DNA breakdown due to endonuclease activity.
CD-Length = 166 residues,  95.2% aligned
Score =  173 bits (440), Expect = 4e-44
Identities = 87/165 (52%), Positives = 104/165 (63%), Gaps = 10/165 (6%)






   
Genomic Context:






   
Comment:
568/573 (99%) identical in C-terminal 573 amino acids compared to PAO1 PA3866.
Missing 191 N-terminal amino acids of PAO1 PA3866.


















    ReferenceID:12787




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30092




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38106




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:6494_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6494
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
7995
                           PA3866_tr
                           translation of PA3866
                           764
                           72.64
                           72.90
                           205
                           2743.0
     

     
     
199905
                           gb|AAG07253.1|AE004804_1
                           (AE004804) pyocin protein [Pseudomonas aeruginosa PAO1]
                           764
                           72.64
                           72.90
                           205
                           2743.0
     

     
     
199906
                           emb|CAA54958.1|
                           (X77996) killer protein of pyocin S3 [Pseudomonas aeruginosa]
                           801
                           56.92
                           59.55
                           277
                           2131.0
     

     
     
199907
                           dbj|BAA02201.1|
                           (D12707) killer protein of pyocin S1 [Pseudomonas aeruginosa]
                           716
                           26.95
                           38.82
                           255
                           683.0
     

     
     
199909
                           gb|AAA98051.1|
                           (M30445) structural protein [Plasmid ColE5-099]
                           596
                           11.07
                           13.75
                           458
                           254.0
     

     






      

          
GC ORFID: 49496How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 3757693Stop: 3756014

          
Length: 1680


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3866 translation of PA3866Homolog Bit Score 1061.0

          
Other Homologs:  t_PA3866 translation of PA3866 (BLASTP 1061)

          
GC ORF Sequence

           
ATGGAGGCGCAAGCCCAGCAGGTCACTCAACAGCTCGGTGCCGACTTCAACGCGATCACCACTCCGACCGCC 
ACCAAGGTCCAGCAACGTGTGAAAGCAGTGGATGCTAGTCTGTCTCAGGTTTCCACCCAGGTCAGTGGTGCA 
GTCGCCTCGGCAACCCAGGCGGTGCAGGTCAAGACAGCGCAAGCCCAGCAACAAGCCAACAGCCAGATTTCG 
TCCAGCCAGAACCTGATCAGCGCGGCATTTCGGAATCAGATTGCCTTGGCAGCACAGGCCAGTGGAGAAGCC 
CAGGTCACGGCTTTCAACACCAAAGTCAAGCAGATCGTCAACGAAGCCACGGCGTTTTCGACAGCACGAAAA 
CAAGCGCTGGCTCAGTTTGCTGCCAATGCGGCCGCCGAGGTCGAAACCGAGGTCAACAGGCTAACCGCCCAG 
GTCAAGGCCAGCCCCACCAAGGCGGCTTCGCAAGCTGCACAAGCAACCCTTCGACAATTCACTGAAAGCACC 
TATGCCAAGGTGAACACCTATGCCCAGCAGACCGAAGCAGCGCTGCAGGCCAAAGCCAAGGAGGTTTCGGCA 
GCGGTAGCCGAGGCACAGCGTGCGGCGACCAACGATTTGCAAAAAACAGCCGATGTAGTACCAGGTCAGATC 
ATTCAGGCTGCGAACACCTTGGCGATGCCGGCTACCTCTACGCCGCTGGTTTTGGTGGCCGGGTTTGGTAGT 
GCGGCAGTGGAGACGGCTCGCCTGGCGGCTTCCCTAACGAATGCTGTCAATCGCCTGGTTCAGATTGCTGGT 
AGTGGACCCGGCGCATACGTTGCCACCTTCGCGGTGCTGTCGCTGTATTCCGACCAGGCTGGTAAAGACAGT 
GACAAGGTTCCTGCCGGCGTTCGCAATGCATTGGCACTGGAGGCGTCCGCTCTGGGGCTTCCTGGCACGGCT 
GATTTGCAAAGCGTCGCCAAGGCAGGTGGCACGGTTGATATGCCGGTACGACTCACGAGTGCTGCACAAGAG 
AGCCCGAGTGGTAAATCGCAGATTGCCGCGATGTTGACCAACGGTGCAACTGTCCCCAAGGGCGTGCCTGTT 
CGCGCCGCGACCCTCAATGCTGCGACGGGCCGGTATGAGGTGACGGTTCCCGCAAAGTCCACCGTGCCGAAT 
ACACCACCGCTGATCTTGACCTGGACCCCTGCCACCCCTCCAGGAAGCCAGAACCCCTCAAGCACCACTCCG 
GTCGTACCGCAGCCGGTTCCGGTGTATGAGGGAGCAACGATCACCCCAGTACAGGCCGAGCCGGAGTCCTAT 
CCGGGAGTGCCGTTAGATCTGGACGATCTGATTGTGATCTTCCCGGTGGGCTCGGGGGTTAAGCCAATCTAT 
ATAATGTTCAATCATAACCCTCATGATGTTGTTATTGATGATAAGATCAGAGAGCAAATTCCAATTAGAGGG 
TGGACTGAGGTTGAAATTAGAGATGTTATCGGAAAAGGTGCAACAGGTAGTTCTTCAGATAAGCGTCGGCCT 
AATAAGACGATAGATGGGCTGGGGCGGAATGACTCTGCAACAGTATATGGAAGTCCTCCTAATGGATATATA 
GTTGTGAACGATAGGACAGGTGAAATTATTCAAATTGCAGATCGGAACGATTCCGCATGGGCGGTTGACGGG 
CGAATCATCTGGAGAAATAAGTAA