ViewGene
Locus Name:
GeneID:
Location:

GeneID:647
TrivialName: PA14_66920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66920
PAO1 Ortholog Locus:PA5065
Sequence Length:1602
Protein Length:533
Start: 5975461
Stop: 5977062
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08450.1|AE004919_11 (AE004919) ubiquinone biosynthetic protein UbiB [Pseudomonas aeruginosa PAO1]
Identities = 532/533 (99%), Positives = 532/533 (99%)
Sequence:
ATGAAGCTCCTCGCTTTCCGCCGCCTCCTGCGCATCCAGCGCGTCGTCATCCGCTACCGCCTGGACGACCTG
ATCCTCGAACTGCCGATGCTGCCCTGGTGGCTGCGCCTGCTCGGTGCCACCCTGCCCTGGCGCTGGCTGCCG
CGGCGCAAGCTGGAACTGACCCGCGGCGCGCGCCTGCGCCTGGCGCTGCAGGACCTCGGACCGATCTTCATC
AAGTTCGGCCAGATTCTCTCCACCCGCCGCGACCTGCTGCCCGACGACATCGCCAACGAACTGGCCTGGCTG
CAGGACAAGGTGCCGCCCTTCCCGCCGGAGCTGGCGGTCAAGCGCATCGAGGAACAACTGGGCGCGAAGATC
GAACAGGTCTTCGCCCGCTTCGAACGCGAACCGCTGGCCTCTGCCTCGGTGGCCCAGGTCCACGCCGCGCGG
CTGAAGAGCGGCGAGGAAGTGGTGGTGAAGGTGATCCGGCCGAACCTGGAACCGGTGATCCGCTCCGACATC
GCCTGGCTGTTCATCCTCGCCCGCCTCGCCGAGCGGGTCTCCAGCGAGGCGCGGCGCCTGCACCCCGTGGAA
GTGGTCAGCGACTACGAGAAAACCATCGTCGACGAACTCGACCTGCTCCGCGAGGCAGCCAACGCCTCGCAG
CTGCGGCGCAACTTCGAAGGCTCGCCGCTGCTCTACGTGCCACAGGTCTACTGGGACTGGTGCCGGCCCAAG
GTGCTGGTGATGGAGCGCATCTACGGCATCCCGGTGACCGACCTGGAAACCCTGCGCGACCAGCGCACCGAC
TTCAAGGCACTGGCCGAGCGCGGCGTGGAGATCTTCTTCACCCAGGTGTTCCGCGACAGCTTCTTCCATGCC
GACATGCACCCCGGCAACATCTTCGTCAGCACCCGCGCGCCCTGGAGCCCGCAATACATCGCGGTGGACTGC
GGCATCGTCGGCAGCCTGACCGACGAGGACCAGGACTACCTGGCGCGCAACCTGATCGCCTTCTTCAAGCGC
GACTACCGCAAGGTCGCCCAACTGCATATCGACTCCGGCTGGGTCCCGGCGGAAACCAAGGTCAACGACTTC
GAGGCGGCGATCCGTACCGTCTGCGAACCGATCTTCGAGAAGCCGCTGAAGGACATCTCCTTCGGCCAGGTC
CTGCTGCGCCTGTTCCAGACCGCGCGTCGTTTCAACATGGAGATCCAGCCGCAACTGGTGCTGCTGCAGAAG
ACCCTGCTGAACATCGAAGGCCTCGGCCGCCAGCTCTACCCGGAACTCGACCTGTGGGCCACCGCCCAGCCG
TTCCTGGAGCGCTGGATGCGCGAACGGGTCAGCCCGAAGCAGTTGCTGCGCAATTTCCAGCAACAGGTCGAG
CAGGTGCCGCACCTGTCGCAAATGGCCCGCGACACCCTCGAACGCCTGTCACAACCGCACGCCCACAATGCG
CCGCCGCCGGAGTGGAAAGGTTCCCGCCACGACTGGCTCGGGCGCCTGGTGGGCGCGGTGCTGCTGGTCGGC
GCCGCCGAGGTCGGCCTGGGTCAGCAGCTGGAAGCCTGGCCGGCGTGGGTGATGCTGGCCGGCGGGGTGTTC
CTGATCCTGCGCCGCTAG
Translation:
MKLLAFRRLLRIQRVVIRYRLDDLILELPMLPWWLRLLGATLPWRWLPRRKLELTRGARLRLALQDLGPIFI
KFGQILSTRRDLLPDDIANELAWLQDKVPPFPPELAVKRIEEQLGAKIEQVFARFEREPLASASVAQVHAAR
LKSGEEVVVKVIRPNLEPVIRSDIAWLFILARLAERVSSEARRLHPVEVVSDYEKTIVDELDLLREAANASQ
LRRNFEGSPLLYVPQVYWDWCRPKVLVMERIYGIPVTDLETLRDQRTDFKALAERGVEIFFTQVFRDSFFHA
DMHPGNIFVSTRAPWSPQYIAVDCGIVGSLTDEDQDYLARNLIAFFKRDYRKVAQLHIDSGWVPAETKVNDF
EAAIRTVCEPIFEKPLKDISFGQVLLRLFQTARRFNMEIQPQLVLLQKTLLNIEGLGRQLYPELDLWATAQP
FLERWMRERVSPKQLLRNFQQQVEQVPHLSQMARDTLERLSQPHAHNAPPPEWKGSRHDWLGRLVGAVLLVG
AAEVGLGQQLEAWPAWVMLAGGVFLILRR*
AnnotationID:656GeneID:647
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 19:07:26
Gene Name:ubiB
Confidence Code:2
GeneProduct:ubiquinone biosynthesis protein UbiB
Cell Localization:()
Synonyms:yigR,aarF
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0661
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58578.1| (AE016874) ubiquinone biosynthesis protein UbiB [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 539

 Score =  826 bits (2134), Expect = 0.0
 Identities = 413/541 (76%), Positives = 464/541 (85%), Gaps = 10/541 (1%)

>gb|AAL22816.1| (AE008885) putative regulator in ubiquinone biosynthesis
           [Salmonella typhimurium LT2]
          Length = 546

 Score =  559 bits (1440), Expect = e-158
 Identities = 304/545 (55%), Positives = 378/545 (69%), Gaps = 39/545 (7%)

>gb|AAO70853.1| (AE016845) ubiquinone biosynthesis protein [Salmonella enterica
           subsp. enterica serovar Typhi Ty2]
          Length = 546

 Score =  559 bits (1440), Expect = e-158
 Identities = 304/545 (55%), Positives = 378/545 (69%), Gaps = 39/545 (7%)






 
Structural Features:
COG0661, AarF, Predicted unusual protein kinase
CD-Length = 517 residues,  99.8% aligned
Score =  517 bits (1332), Expect = 1e-147
Identities = 240/520 (46%), Positives = 324/520 (62%), Gaps = 14/520 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:1297




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:18439




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35000




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:647_cdd.html


    		




 
URL:

















    ReferenceID:35826




  
Author/Investigator(s):
        Poon WW, Davis DE, Ha HT, Jonassen T, Rather PN, Clarke CF.





  
Title:
        Identification of Escherichia coli ubiB, a gene required for the first monooxygenase step in ubiquinone biosynthesis.




  
PubMed:
        10960098


MedLine:
        




    
Source:
J Bacteriol. 2000 Sep;182(18):5139-46.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10960098









           
Homologs By Global Alignment 

           

       



     
Gene ID:647
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
1098
                           PA5065_tr
                           translation of PA5065
                           533
                           99.81
                           99.81
                           0
                           2757.0
     

     
     
41685
                           gb|AAG08450.1|AE004919_11
                           (AE004919) ubiquinone biosynthetic protein UbiB [Pseudomonas aeruginosa PAO1]
                           533
                           99.81
                           99.81
                           0
                           2757.0
     

     
     
41686
                           gb|AAO58578.1|
                           (AE016874) ubiquinone biosynthesis protein UbiB [Pseudomonas syringae pv. tomato str. DC3000]
                           541
                           76.34
                           85.76
                           10
                           2143.0
     

     
     
41691
                           gb|AAN83217.1|AE016770_17
                           (AE016770) Probable ubiquinone biosynthesis protein ubiB [Escherichia coli CFT073]
                           568
                           54.92
                           69.19
                           57
                           1469.0
     

     
     
41692
                           gb|AAP18850.1|
                           (AE016990) hypothetical protein S3841 [Shigella flexneri 2a str. 2457T]
                           568
                           54.75
                           69.01
                           57
                           1464.0
     

     






      

          
GC ORFID: 43649How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5065Glimmer Score: 

          
Start: 5239145Stop: 5240746

          
Length: 1602


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5065 translation of PA5065Homolog Bit Score 1066.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3796 (ORF: BLASTX 830.0), t_PA5065 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCTCCTCGCTTTCCGCCGCCTCCTGCGCATCCAGCGCGTCGTCATCCGCTACCGCCTGGACGACCTG 
ATCCTCGAACTGCCGATGCTGCCCTGGTGGCTGCGCCTGCTCGGTGCCACCCTGCCCTGGCGCTGGCTGCCG 
CGGCGCAAGCTGGAACTGACCCGCGGCGCGCGCCTGCGCCTGGCGCTGCAGGACCTCGGACCGATCTTCATC 
AAGTTCGGCCAGATTCTCTCCACCCGCCGCGACCTGCTGCCCGACGACATCGCCAACGAACTGGCCTGGCTG 
CAGGACAAGGTGCCGCCCTTCCCGCCGGAGCTGGCGGTCAAGCGCATCGAGGAACAACTGGGCGCGAAGATC 
GAACAGGTCTTCGCCCGCTTCGAACGCGAACCGCTGGCCTCTGCCTCGGTGGCCCAGGTCCACGCCGCGCGG 
CTGAAGAGCGGCGAGGAAGTGGTGGTGAAGGTGATCCGGCCGAACCTGGAACCGGTGATCCGCTCCGACATC 
GCCTGGCTGTTCATCCTCGCCCGCCTCGCCGAGCGGGTCTCCAGCGAGGCGCGGCGCCTGCACCCCGTGGAA 
GTGGTCAGCGACTACGAGAAAACCATCGTCGACGAACTCGACCTGCTCCGCGAGGCAGCCAACGCCTCGCAG 
CTGCGGCGCAACTTCGAAGGCTCGCCGCTGCTCTACGTGCCACAGGTCTACTGGGACTGGTGCCGGCCCAAG 
GTGCTGGTGATGGAGCGCATCTACGGCATCCCGGTGACCGACCTGGAAACCCTGCGCGACCAGCGCACCGAC 
TTCAAGGCACTGGCCGAGCGCGGCGTGGAGATCTTCTTCACCCAGGTGTTCCGCGACAGCTTCTTCCATGCC 
GACATGCACCCCGGCAACATCTTCGTCAGCACCCGCGCGCCCTGGAGCCCGCAATACATCGCGGTGGACTGC 
GGCATCGTCGGCAGCCTGACCGACGAGGACCAGGACTACCTGGCGCGCAACCTGATCGCCTTCTTCAAGCGC 
GACTACCGCAAGGTCGCCCAACTGCATATCGACTCCGGCTGGGTCCCGGCGGAAACCAAGGTCAACGACTTC 
GAGGCGGCGATCCGTACCGTCTGCGAACCGATCTTCGAGAAGCCGCTGAAGGACATCTCCTTCGGCCAGGTC 
CTGCTGCGCCTGTTCCAGACCGCGCGTCGTTTCAACATGGAGATCCAGCCGCAACTGGTGCTGCTGCAGAAG 
ACCCTGCTGAACATCGAAGGCCTCGGCCGCCAGCTCTACCCGGAACTCGACCTGTGGGCCACCGCCCAGCCG 
TTCCTGGAGCGCTGGATGCGCGAACGGGTCAGCCCGAAGCAGTTGCTGCGCAATTTCCAGCAACAGGTCGAG 
CAGGTGCCGCACCTGTCGCAAATGGCCCGCGACACCCTCGAACGCCTGTCACAACCGCACGCCCACAATGCG 
CCGCCGCCGGAGTGGAAAGGTTCCCGCCACGACTGGCTCGGGCGCCTGGTGGGCGCGGTGCTGCTGGTCGGC 
GCCGCCGAGGTCGGCCTGGGTCAGCAGCTGGAAGCCTGGCCGGCGTGGGTGATGCTGGCCGGCGGGGTGTTC 
CTGATCCTGCGCCGCTAG