ViewGene
Locus Name:
GeneID:
Location:

GeneID:6449
TrivialName: PA14_15460
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_15460
PAO1 Ortholog Locus:
Sequence Length:1686
Protein Length:561
Start: 1310388
Stop: 1308703
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: emb|CAA77323.1| (Z00027) merA protein (mercuric reductase) [Pseudomonas aeruginosa]
Identities = 559/561 (99%), Positives = 560/561 (99%)
Sequence:
ATGACCCATCTAAAAATCACCGGCATGACTTGCGACTCGTGCGCGGCGCACGTCAAGGAAGCGCTGGAAAAA
GTGCCAGGCGTGCAGTCGGCGGTGGTGTCCTATCCGAAGGGCACAGCGCAACTCGCCATCGTGCCGGGCACA
TCGCCGGACGCGCTGACTGCCGCCGTGGCCGGACTGGGCTACAAGGCAACGCTAGCCGATGCGCCACTGGCG
GACAACCGCGTCGGACTGCTCGACAAGGTGCGGGGATGGATGGCCGCCGCCGAAAAGCACAGTGGCAACGAG
CCCCCGGTGCAGGTAGCGGTCATTGGCAGCGGTGGAGCCGCGATGGCGGCGGCGCTGAAGGCCGTCGAGCAA
GGCGCGCAGGTCACGCTGATCGAGCGCGGCACCATCGGCGGCACCTGCGTCAATGTCGGCTGTGTGCCGTCC
AAGATCATGATCCGCGCCGCCCACATCGCCCATCTGCGCCGGGAAAGCCCGTTCGATGGCGGTATTGCGGCA
ACTGTGCCTACGATTGACCGCAGTAAGCTGCTGGCCCAGCAGCAGGCCCGCGTCGACGAACTGCGGCACGCC
AAGTACGAAGGCATCCTGGGCGGTAATCCGGCCATCACCGTTGTGCACGGTGAGGCGCGCTTCAAGGACGAC
CAGAGCCTTACCGTCCGTTTGAACGAGGGTGGCGAGCGCGTCGTGATGTTCGACCGCTGCCTGGTCGCCGCG
GGTGCCAGCCCGGCGGTCCCGCCGATTCCGGGCTTGAAAGAGTCACCCTACTGGACTTCCACCGAGGCCCTG
GCGAGCGACACCATTCCCGAACGCCTTGCCGTAATCGGCTCGTCGGTGGTGGCGCTGGAGCTGGCGCAAGCC
TTTGCCCGGCTGGGCAGCAAGGTCACGGTCCTGGCGCGCAATACCTTGTTCTTCCGTGAAGACCCGGCCATC
GGCGAGGCGGTGACAGCCGCTTTCCGTGCCGAGGGCATCGAGGTGCTGGAGCACACGCAAGCCAGCCAGGTC
GCCCATATGGACGGTGAATTCGTGCTGACCACCACGCACGGTGAATTGCGCGCCGACAAACTGCTGGTTGCC
ACCGGTCGGACACCGAACACGCGCAGCCTCGCGCTGGACGCAGCGGGGGTCACTGTCAATGCGCAAGGTGCC
ATCGTCATCGACCAAGGCATGCGCACGAGCAACCCGAACATCTACGCGGCCGGCGACTGCACCGACCAGCCG
CAGTTCGTCTATGTGGCGGCAGCGGCCGGCACCCGTGCCGCGATCAACATGACCGGCGGCGATGCGGCGCTC
GACCTGACCGCAATGCCGGCCGTGGTGTTCACCGATCCGCAAGTGGCGACCGTGGGCTACAGCGAGGCGGAA
GCCCACCACGACGGGATCGAGACCGACAGCCGCACCTTGACCTTGGACAACGTGCCGCGTGCGCTCGCCAAC
TTCGACACACGCGGCTTCATCAAGTTGGTTATCGAGGAAGGCAGCCATCGGCTGATCGGCGTACAGGCGGTC
GCGCCGGAAGCGGGTGAACTGATCCAGACGGCGGCTCTGGCCATTCGCAACCGCATGACGGTGCAGGAACTG
GCCGACCAGTTGTTCCCCTACCTGACGATGGTCGAGGGGTTGAAGCTCGCGGCGCAGACCTTCAACAAGGAT
GTGAAGCAGCTTTCCTGCTGCGCCGGGTGA
Translation:
MTHLKITGMTCDSCAAHVKEALEKVPGVQSAVVSYPKGTAQLAIVPGTSPDALTAAVAGLGYKATLADAPLA
DNRVGLLDKVRGWMAAAEKHSGNEPPVQVAVIGSGGAAMAAALKAVEQGAQVTLIERGTIGGTCVNVGCVPS
KIMIRAAHIAHLRRESPFDGGIAATVPTIDRSKLLAQQQARVDELRHAKYEGILGGNPAITVVHGEARFKDD
QSLTVRLNEGGERVVMFDRCLVAAGASPAVPPIPGLKESPYWTSTEALASDTIPERLAVIGSSVVALELAQA
FARLGSKVTVLARNTLFFREDPAIGEAVTAAFRAEGIEVLEHTQASQVAHMDGEFVLTTTHGELRADKLLVA
TGRTPNTRSLALDAAGVTVNAQGAIVIDQGMRTSNPNIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAAL
DLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRTLTLDNVPRALANFDTRGFIKLVIEEGSHRLIGVQAV
APEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG*
AnnotationID:6450GeneID:6449
AnnotatorUID: danlee
Modification Date/Time: 2005-10-17 16:04:16
Gene Name:merA
Confidence Code:2
GeneProduct:Mercuric reductase MerA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(1) Adaptation, protection
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1249, pfam00070
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gi|68526838|gb|EAN49808.1|  FAD-dependent pyridine nucleotide-disulphide oxidoreductase:Pyridine 
nucleotide-disulphide oxidoreductase dimerisation region:Heavy metal transport/detoxification protein 
[Ralstonia metallidurans CH34]
     Length=561
     Score = 1090 bits (2819),  Expect = 0.0
     Identities = 560/561 (99%), Positives = 561/561 (100%), Gaps = 0/561 (0%)

gi|43718|emb|CAA77323.1|  merA protein (mercuric reductase) [Pseudomonas aeruginosa]
gi|56068723|emb|CAI11285.1|  mercuric-ion reductase fad flavoprotein [Ralstonia metallidurans CH34]
gi|13449208|ref|NP_085424.1|  Tn501, mercuric reductase [Shigella flexneri]
gi|126988|sp|P00392|MERA_PSEAE  Mercuric reductase (Hg(II) reductase)
     Length=561
     Score = 1088 bits (2815),  Expect = 0.0
     Identities = 559/561 (99%), Positives = 560/561 (99%), Gaps = 0/561 (0%)






 
Structural Features:
COG1249, Lpd, Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) 
component, and related enzymes [Energy production and conversion].
             CD-Length = 454 residues,  94.3% aligned
             Score =  361 bits (928), Expect = 1e-100

pfam00070, Pyr_redox, Pyridine nucleotide-disulphide oxidoreductase. 
             CD-Length = 288 residues,  93.1% aligned
             Score =  144 bits (364), Expect = 3e-35

N-Terminal Match:
cd00371, HMA, Heavy-metal-associated domain (HMA) is a conserved domain of approximately 
30 amino acid residues found in a number of proteins that transport or detoxify heavy metals.
             CD-Length = 63 residues,  98.4% aligned
             Score = 71.8 bits (176), Expect = 2e-13






   
Genomic Context:






   
Comment:


















    ReferenceID:12707




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:30003




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:6449
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
199381
                           emb|CAA77323.1|
                           (Z00027) merA protein (mercuric reductase) [Pseudomonas aeruginosa]
                           561
                           99.64
                           99.82
                           0
                           2815.0
     

     
     
199382
                           gb|AAK18580.1|AF348706_269
                           (AF348706) Tn501, mercuric reductase [Shigella flexneri]
                           561
                           99.64
                           99.82
                           0
                           2815.0
     

     
     
199383
                           emb|CAD97551.1|
                           (AJ564903) mercuric reductase [uncultured bacterium]
                           561
                           99.46
                           99.64
                           0
                           2811.0
     

     
     
199384
                           gb|AAC38220.1|
                           (U80214) MerA [Pseudomonas stutzeri]
                           561
                           98.39
                           99.10
                           0
                           2778.0
     

     
     
199388
                           emb|CAD09750.1|
                           (AL513383) putative mercuric reductase [Salmonella enterica subsp. enterica serovar Typhi]
                           561
                           92.69
                           95.18
                           0
                           2603.0
     

     






      

          
GC ORFID: 49451How Found: Glimmer

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: Glimmer Score: 

          
Start: 3643549Stop: 3645234

          
Length: 1686


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: Homolog Bit Score 

          
Other Homologs:  

          
GC ORF Sequence

           
ATGACCCATCTAAAAATCACCGGCATGACTTGCGACTCGTGCGCGGCGCACGTCAAGGAAGCGCTGGAAAAA 
GTGCCAGGCGTGCAGTCGGCGGTGGTGTCCTATCCGAAGGGCACAGCGCAACTCGCCATCGTGCCGGGCACA 
TCGCCGGACGCGCTGACTGCCGCCGTGGCCGGACTGGGCTACAAGGCAACGCTAGCCGATGCGCCACTGGCG 
GACAACCGCGTCGGACTGCTCGACAAGGTGCGGGGATGGATGGCCGCCGCCGAAAAGCACAGTGGCAACGAG 
CCCCCGGTGCAGGTAGCGGTCATTGGCAGCGGTGGAGCCGCGATGGCGGCGGCGCTGAAGGCCGTCGAGCAA 
GGCGCGCAGGTCACGCTGATCGAGCGCGGCACCATCGGCGGCACCTGCGTCAATGTCGGCTGTGTGCCGTCC 
AAGATCATGATCCGCGCCGCCCACATCGCCCATCTGCGCCGGGAAAGCCCGTTCGATGGCGGTATTGCGGCA 
ACTGTGCCTACGATTGACCGCAGTAAGCTGCTGGCCCAGCAGCAGGCCCGCGTCGACGAACTGCGGCACGCC 
AAGTACGAAGGCATCCTGGGCGGTAATCCGGCCATCACCGTTGTGCACGGTGAGGCGCGCTTCAAGGACGAC 
CAGAGCCTTACCGTCCGTTTGAACGAGGGTGGCGAGCGCGTCGTGATGTTCGACCGCTGCCTGGTCGCCGCG 
GGTGCCAGCCCGGCGGTCCCGCCGATTCCGGGCTTGAAAGAGTCACCCTACTGGACTTCCACCGAGGCCCTG 
GCGAGCGACACCATTCCCGAACGCCTTGCCGTAATCGGCTCGTCGGTGGTGGCGCTGGAGCTGGCGCAAGCC 
TTTGCCCGGCTGGGCAGCAAGGTCACGGTCCTGGCGCGCAATACCTTGTTCTTCCGTGAAGACCCGGCCATC 
GGCGAGGCGGTGACAGCCGCTTTCCGTGCCGAGGGCATCGAGGTGCTGGAGCACACGCAAGCCAGCCAGGTC 
GCCCATATGGACGGTGAATTCGTGCTGACCACCACGCACGGTGAATTGCGCGCCGACAAACTGCTGGTTGCC 
ACCGGTCGGACACCGAACACGCGCAGCCTCGCGCTGGACGCAGCGGGGGTCACTGTCAATGCGCAAGGTGCC 
ATCGTCATCGACCAAGGCATGCGCACGAGCAACCCGAACATCTACGCGGCCGGCGACTGCACCGACCAGCCG 
CAGTTCGTCTATGTGGCGGCAGCGGCCGGCACCCGTGCCGCGATCAACATGACCGGCGGCGATGCGGCGCTC 
GACCTGACCGCAATGCCGGCCGTGGTGTTCACCGATCCGCAAGTGGCGACCGTGGGCTACAGCGAGGCGGAA 
GCCCACCACGACGGGATCGAGACCGACAGCCGCACCTTGACCTTGGACAACGTGCCGCGTGCGCTCGCCAAC 
TTCGACACACGCGGCTTCATCAAGTTGGTTATCGAGGAAGGCAGCCATCGGCTGATCGGCGTACAGGCGGTC 
GCGCCGGAAGCGGGTGAACTGATCCAGACGGCGGCTCTGGCCATTCGCAACCGCATGACGGTGCAGGAACTG 
GCCGACCAGTTGTTCCCCTACCTGACGATGGTCGAGGGGTTGAAGCTCGCGGCGCAGACCTTCAACAAGGAT 
GTGAAGCAGCTTTCCTGCTGCGCCGGGTGA